This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.62 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 74,814 (2.6%) Reads that were too short: 27,478 (0.9%) Reads written (passing filters): 47,336 (1.6%) Total basepairs processed: 881,011,047 bp Total written (filtered): 9,170,625 bp (1.0%) === Adapter 1 === Sequence: GCGAGA; Type: regular 5'; Length: 6; Trimmed: 74814 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 124 714.6 0 124 7 4909 714.6 0 4909 8 4372 714.6 0 4372 9 52 714.6 0 52 10 44 714.6 0 44 11 107 714.6 0 107 12 38 714.6 0 38 13 39 714.6 0 39 14 33 714.6 0 33 15 37 714.6 0 37 16 35 714.6 0 35 17 35 714.6 0 35 18 42 714.6 0 42 19 28 714.6 0 28 20 46 714.6 0 46 21 36 714.6 0 36 22 32 714.6 0 32 23 34 714.6 0 34 24 40 714.6 0 40 25 57 714.6 0 57 26 42 714.6 0 42 27 38 714.6 0 38 28 40 714.6 0 40 29 41 714.6 0 41 30 63 714.6 0 63 31 103 714.6 0 103 32 211 714.6 0 211 33 43 714.6 0 43 34 55 714.6 0 55 35 35 714.6 0 35 36 43 714.6 0 43 37 56 714.6 0 56 38 49 714.6 0 49 39 55 714.6 0 55 40 79 714.6 0 79 41 55 714.6 0 55 42 77 714.6 0 77 43 60 714.6 0 60 44 64 714.6 0 64 45 65 714.6 0 65 46 62 714.6 0 62 47 65 714.6 0 65 48 80 714.6 0 80 49 75 714.6 0 75 50 346 714.6 0 346 51 668 714.6 0 668 52 604 714.6 0 604 53 67 714.6 0 67 54 77 714.6 0 77 55 63 714.6 0 63 56 61 714.6 0 61 57 66 714.6 0 66 58 68 714.6 0 68 59 299 714.6 0 299 60 71 714.6 0 71 61 97 714.6 0 97 62 57 714.6 0 57 63 52 714.6 0 52 64 67 714.6 0 67 65 74 714.6 0 74 66 50 714.6 0 50 67 59 714.6 0 59 68 74 714.6 0 74 69 83 714.6 0 83 70 93 714.6 0 93 71 57 714.6 0 57 72 89 714.6 0 89 73 90 714.6 0 90 74 915 714.6 0 915 75 1122 714.6 0 1122 76 459 714.6 0 459 77 82 714.6 0 82 78 188 714.6 0 188 79 163 714.6 0 163 80 242 714.6 0 242 81 144 714.6 0 144 82 190 714.6 0 190 83 202 714.6 0 202 84 218 714.6 0 218 85 385 714.6 0 385 86 188 714.6 0 188 87 274 714.6 0 274 88 106 714.6 0 106 89 169 714.6 0 169 90 82 714.6 0 82 91 176 714.6 0 176 92 127 714.6 0 127 93 177 714.6 0 177 94 245 714.6 0 245 95 208 714.6 0 208 96 157 714.6 0 157 97 160 714.6 0 160 98 142 714.6 0 142 99 88 714.6 0 88 100 84 714.6 0 84 101 61 714.6 0 61 102 99 714.6 0 99 103 81 714.6 0 81 104 63 714.6 0 63 105 50 714.6 0 50 106 38 714.6 0 38 107 26 714.6 0 26 108 29 714.6 0 29 109 61 714.6 0 61 110 40 714.6 0 40 111 84 714.6 0 84 112 124 714.6 0 124 113 102 714.6 0 102 114 302 714.6 0 302 115 215 714.6 0 215 116 297 714.6 0 297 117 169 714.6 0 169 118 213 714.6 0 213 119 189 714.6 0 189 120 159 714.6 0 159 121 142 714.6 0 142 122 182 714.6 0 182 123 187 714.6 0 187 124 165 714.6 0 165 125 182 714.6 0 182 126 426 714.6 0 426 127 282 714.6 0 282 128 166 714.6 0 166 129 187 714.6 0 187 130 244 714.6 0 244 131 214 714.6 0 214 132 292 714.6 0 292 133 211 714.6 0 211 134 239 714.6 0 239 135 629 714.6 0 629 136 451 714.6 0 451 137 653 714.6 0 653 138 178 714.6 0 178 139 163 714.6 0 163 140 167 714.6 0 167 141 173 714.6 0 173 142 179 714.6 0 179 143 144 714.6 0 144 144 269 714.6 0 269 145 186 714.6 0 186 146 231 714.6 0 231 147 174 714.6 0 174 148 179 714.6 0 179 149 136 714.6 0 136 150 139 714.6 0 139 151 111 714.6 0 111 152 112 714.6 0 112 153 150 714.6 0 150 154 166 714.6 0 166 155 165 714.6 0 165 156 138 714.6 0 138 157 189 714.6 0 189 158 170 714.6 0 170 159 179 714.6 0 179 160 186 714.6 0 186 161 166 714.6 0 166 162 175 714.6 0 175 163 157 714.6 0 157 164 216 714.6 0 216 165 323 714.6 0 323 166 283 714.6 0 283 167 213 714.6 0 213 168 306 714.6 0 306 169 350 714.6 0 350 170 199 714.6 0 199 171 234 714.6 0 234 172 217 714.6 0 217 173 224 714.6 0 224 174 256 714.6 0 256 175 213 714.6 0 213 176 167 714.6 0 167 177 183 714.6 0 183 178 182 714.6 0 182 179 191 714.6 0 191 180 220 714.6 0 220 181 265 714.6 0 265 182 233 714.6 0 233 183 248 714.6 0 248 184 213 714.6 0 213 185 293 714.6 0 293 186 281 714.6 0 281 187 235 714.6 0 235 188 164 714.6 0 164 189 175 714.6 0 175 190 184 714.6 0 184 191 270 714.6 0 270 192 343 714.6 0 343 193 563 714.6 0 563 194 212 714.6 0 212 195 469 714.6 0 469 196 329 714.6 0 329 197 881 714.6 0 881 198 2621 714.6 0 2621 199 650 714.6 0 650 200 1068 714.6 0 1068 201 305 714.6 0 305 202 166 714.6 0 166 203 152 714.6 0 152 204 401 714.6 0 401 205 264 714.6 0 264 206 207 714.6 0 207 207 197 714.6 0 197 208 155 714.6 0 155 209 163 714.6 0 163 210 155 714.6 0 155 211 190 714.6 0 190 212 214 714.6 0 214 213 232 714.6 0 232 214 209 714.6 0 209 215 246 714.6 0 246 216 444 714.6 0 444 217 586 714.6 0 586 218 978 714.6 0 978 219 404 714.6 0 404 220 321 714.6 0 321 221 296 714.6 0 296 222 211 714.6 0 211 223 130 714.6 0 130 224 150 714.6 0 150 225 212 714.6 0 212 226 189 714.6 0 189 227 222 714.6 0 222 228 236 714.6 0 236 229 288 714.6 0 288 230 193 714.6 0 193 231 166 714.6 0 166 232 143 714.6 0 143 233 124 714.6 0 124 234 138 714.6 0 138 235 168 714.6 0 168 236 191 714.6 0 191 237 366 714.6 0 366 238 227 714.6 0 227 239 325 714.6 0 325 240 251 714.6 0 251 241 232 714.6 0 232 242 129 714.6 0 129 243 116 714.6 0 116 244 127 714.6 0 127 245 208 714.6 0 208 246 342 714.6 0 342 247 1369 714.6 0 1369 248 3436 714.6 0 3436 249 1593 714.6 0 1593 250 458 714.6 0 458 251 78 714.6 0 78 252 86 714.6 0 86 253 86 714.6 0 86 254 171 714.6 0 171 255 201 714.6 0 201 256 259 714.6 0 259 257 294 714.6 0 294 258 351 714.6 0 351 259 195 714.6 0 195 260 109 714.6 0 109 261 56 714.6 0 56 262 74 714.6 0 74 263 87 714.6 0 87 264 125 714.6 0 125 265 153 714.6 0 153 266 202 714.6 0 202 267 266 714.6 0 266 268 315 714.6 0 315 269 160 714.6 0 160 270 172 714.6 0 172 271 109 714.6 0 109 272 107 714.6 0 107 273 136 714.6 0 136 274 212 714.6 0 212 275 251 714.6 0 251 276 144 714.6 0 144 277 87 714.6 0 87 278 91 714.6 0 91 279 164 714.6 0 164 280 209 714.6 0 209 281 265 714.6 0 265 282 135 714.6 0 135 283 136 714.6 0 136 284 128 714.6 0 128 285 168 714.6 0 168 286 239 714.6 0 239 287 336 714.6 0 336 288 198 714.6 0 198 289 198 714.6 0 198 290 235 714.6 0 235 291 373 714.6 0 373 292 249 714.6 0 249 293 239 714.6 0 239 294 305 714.6 0 305 295 250 714.6 0 250 296 173 714.6 0 173 297 171 714.6 0 171 298 209 714.6 0 209 299 194 714.6 0 194 300 172 714.6 0 172 301 235 714.6 0 235 Finished in 1.81 s (38 us/read; 1.57 M reads/minute). === Summary === Total reads processed: 47,336 Reads with adapters: 5,024 (10.6%) Reads that were too short: 140 (0.3%) Reads written (passing filters): 4,884 (10.3%) Total basepairs processed: 9,170,625 bp Total written (filtered): 1,340,817 bp (14.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5024 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 38 0.0 3 0 30 4 4 17 4730 0.0 3 4599 115 13 3 18 21 0.0 3 1 9 6 5 19 14 0.0 3 0 0 7 7 20 3 0.0 3 0 0 0 3 26 3 0.0 3 0 0 0 3 27 4 0.0 3 0 0 0 4 35 2 0.0 3 0 0 0 2 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 1 38 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 5 0.0 3 0 0 0 5 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 62 3 0.0 3 0 0 0 3 63 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 78 3 0.0 3 0 0 0 3 81 1 0.0 3 0 0 0 1 87 28 0.0 3 0 0 0 28 88 1 0.0 3 0 0 0 1 89 5 0.0 3 0 0 0 5 90 16 0.0 3 0 1 1 14 92 3 0.0 3 0 0 0 3 93 2 0.0 3 0 0 0 2 95 4 0.0 3 0 0 0 4 96 1 0.0 3 0 0 0 1 97 12 0.0 3 0 0 0 12 100 2 0.0 3 0 0 0 2 103 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 8 0.0 3 0 0 0 8 118 4 0.0 3 0 0 0 4 120 8 0.0 3 0 0 1 7 121 4 0.0 3 0 0 0 4 123 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 217 56 0.0 3 0 0 4 52 218 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.18 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 4,884 Reads with adapters: 405 (8.3%) Reads written (passing filters): 4,884 (100.0%) Total basepairs processed: 1,340,817 bp Total written (filtered): 1,339,600 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 405 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 25.4% C: 59.3% G: 11.4% T: 4.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 403 76.3 0 403 4 2 19.1 0 2