This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.23 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 210,162 (7.2%) Reads that were too short: 116,905 (4.0%) Reads written (passing filters): 93,257 (3.2%) Total basepairs processed: 881,011,047 bp Total written (filtered): 20,613,685 bp (2.3%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 210162 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 618 714.6 0 618 7 713 714.6 0 713 8 36506 714.6 0 36506 9 4705 714.6 0 4705 10 50 714.6 0 50 11 46 714.6 0 46 12 31 714.6 0 31 13 49 714.6 0 49 14 53 714.6 0 53 15 34 714.6 0 34 16 44 714.6 0 44 17 59 714.6 0 59 18 494 714.6 0 494 19 86 714.6 0 86 20 28 714.6 0 28 21 41 714.6 0 41 22 43 714.6 0 43 23 33 714.6 0 33 24 35 714.6 0 35 25 39 714.6 0 39 26 30 714.6 0 30 27 17 714.6 0 17 28 33 714.6 0 33 29 30 714.6 0 30 30 37 714.6 0 37 31 49 714.6 0 49 32 35 714.6 0 35 33 31 714.6 0 31 34 27 714.6 0 27 35 35 714.6 0 35 36 33 714.6 0 33 37 43 714.6 0 43 38 59 714.6 0 59 39 45 714.6 0 45 40 32 714.6 0 32 41 45 714.6 0 45 42 41 714.6 0 41 43 71 714.6 0 71 44 250 714.6 0 250 45 116 714.6 0 116 46 3506 714.6 0 3506 47 402 714.6 0 402 48 55 714.6 0 55 49 32 714.6 0 32 50 33 714.6 0 33 51 31 714.6 0 31 52 56 714.6 0 56 53 45 714.6 0 45 54 136 714.6 0 136 55 34 714.6 0 34 56 112 714.6 0 112 57 33 714.6 0 33 58 34 714.6 0 34 59 34 714.6 0 34 60 27 714.6 0 27 61 29 714.6 0 29 62 47 714.6 0 47 63 25 714.6 0 25 64 35 714.6 0 35 65 33 714.6 0 33 66 48 714.6 0 48 67 31 714.6 0 31 68 37 714.6 0 37 69 35 714.6 0 35 70 34 714.6 0 34 71 120 714.6 0 120 72 47 714.6 0 47 73 38 714.6 0 38 74 38 714.6 0 38 75 41 714.6 0 41 76 44 714.6 0 44 77 39 714.6 0 39 78 36 714.6 0 36 79 59 714.6 0 59 80 25 714.6 0 25 81 61 714.6 0 61 82 127 714.6 0 127 83 67 714.6 0 67 84 108 714.6 0 108 85 164 714.6 0 164 86 111 714.6 0 111 87 73 714.6 0 73 88 80 714.6 0 80 89 88 714.6 0 88 90 96 714.6 0 96 91 116 714.6 0 116 92 115 714.6 0 115 93 115 714.6 0 115 94 321 714.6 0 321 95 193 714.6 0 193 96 225 714.6 0 225 97 227 714.6 0 227 98 180 714.6 0 180 99 439 714.6 0 439 100 163 714.6 0 163 101 83 714.6 0 83 102 138 714.6 0 138 103 111 714.6 0 111 104 81 714.6 0 81 105 41 714.6 0 41 106 92 714.6 0 92 107 106 714.6 0 106 108 171 714.6 0 171 109 119 714.6 0 119 110 171 714.6 0 171 111 99 714.6 0 99 112 142 714.6 0 142 113 94 714.6 0 94 114 147 714.6 0 147 115 276 714.6 0 276 116 219 714.6 0 219 117 172 714.6 0 172 118 172 714.6 0 172 119 253 714.6 0 253 120 287 714.6 0 287 121 248 714.6 0 248 122 148 714.6 0 148 123 172 714.6 0 172 124 231 714.6 0 231 125 362 714.6 0 362 126 227 714.6 0 227 127 313 714.6 0 313 128 220 714.6 0 220 129 251 714.6 0 251 130 256 714.6 0 256 131 235 714.6 0 235 132 166 714.6 0 166 133 220 714.6 0 220 134 544 714.6 0 544 135 751 714.6 0 751 136 549 714.6 0 549 137 505 714.6 0 505 138 292 714.6 0 292 139 220 714.6 0 220 140 250 714.6 0 250 141 252 714.6 0 252 142 205 714.6 0 205 143 206 714.6 0 206 144 224 714.6 0 224 145 715 714.6 0 715 146 670 714.6 0 670 147 653 714.6 0 653 148 280 714.6 0 280 149 222 714.6 0 222 150 222 714.6 0 222 151 265 714.6 0 265 152 302 714.6 0 302 153 381 714.6 0 381 154 428 714.6 0 428 155 417 714.6 0 417 156 380 714.6 0 380 157 455 714.6 0 455 158 389 714.6 0 389 159 365 714.6 0 365 160 304 714.6 0 304 161 277 714.6 0 277 162 270 714.6 0 270 163 242 714.6 0 242 164 319 714.6 0 319 165 920 714.6 0 920 166 758 714.6 0 758 167 436 714.6 0 436 168 295 714.6 0 295 169 260 714.6 0 260 170 285 714.6 0 285 171 262 714.6 0 262 172 338 714.6 0 338 173 1488 714.6 0 1488 174 2485 714.6 0 2485 175 1188 714.6 0 1188 176 624 714.6 0 624 177 242 714.6 0 242 178 261 714.6 0 261 179 363 714.6 0 363 180 501 714.6 0 501 181 1918 714.6 0 1918 182 793 714.6 0 793 183 752 714.6 0 752 184 700 714.6 0 700 185 405 714.6 0 405 186 796 714.6 0 796 187 455 714.6 0 455 188 254 714.6 0 254 189 241 714.6 0 241 190 340 714.6 0 340 191 620 714.6 0 620 192 806 714.6 0 806 193 838 714.6 0 838 194 486 714.6 0 486 195 213 714.6 0 213 196 215 714.6 0 215 197 320 714.6 0 320 198 545 714.6 0 545 199 385 714.6 0 385 200 278 714.6 0 278 201 368 714.6 0 368 202 465 714.6 0 465 203 489 714.6 0 489 204 647 714.6 0 647 205 464 714.6 0 464 206 479 714.6 0 479 207 452 714.6 0 452 208 343 714.6 0 343 209 313 714.6 0 313 210 319 714.6 0 319 211 369 714.6 0 369 212 497 714.6 0 497 213 424 714.6 0 424 214 658 714.6 0 658 215 636 714.6 0 636 216 540 714.6 0 540 217 535 714.6 0 535 218 626 714.6 0 626 219 775 714.6 0 775 220 611 714.6 0 611 221 622 714.6 0 622 222 575 714.6 0 575 223 539 714.6 0 539 224 518 714.6 0 518 225 3880 714.6 0 3880 226 4671 714.6 0 4671 227 7073 714.6 0 7073 228 566 714.6 0 566 229 375 714.6 0 375 230 983 714.6 0 983 231 971 714.6 0 971 232 1151 714.6 0 1151 233 611 714.6 0 611 234 865 714.6 0 865 235 647 714.6 0 647 236 528 714.6 0 528 237 607 714.6 0 607 238 585 714.6 0 585 239 559 714.6 0 559 240 452 714.6 0 452 241 518 714.6 0 518 242 547 714.6 0 547 243 535 714.6 0 535 244 458 714.6 0 458 245 477 714.6 0 477 246 1085 714.6 0 1085 247 1259 714.6 0 1259 248 886 714.6 0 886 249 205 714.6 0 205 250 667 714.6 0 667 251 1552 714.6 0 1552 252 2579 714.6 0 2579 253 1215 714.6 0 1215 254 1080 714.6 0 1080 255 714 714.6 0 714 256 649 714.6 0 649 257 566 714.6 0 566 258 521 714.6 0 521 259 347 714.6 0 347 260 412 714.6 0 412 261 509 714.6 0 509 262 555 714.6 0 555 263 480 714.6 0 480 264 477 714.6 0 477 265 539 714.6 0 539 266 957 714.6 0 957 267 740 714.6 0 740 268 344 714.6 0 344 269 320 714.6 0 320 270 479 714.6 0 479 271 821 714.6 0 821 272 758 714.6 0 758 273 595 714.6 0 595 274 624 714.6 0 624 275 689 714.6 0 689 276 543 714.6 0 543 277 618 714.6 0 618 278 867 714.6 0 867 279 1035 714.6 0 1035 280 1180 714.6 0 1180 281 1018 714.6 0 1018 282 791 714.6 0 791 283 657 714.6 0 657 284 750 714.6 0 750 285 1265 714.6 0 1265 286 1184 714.6 0 1184 287 664 714.6 0 664 288 411 714.6 0 411 289 475 714.6 0 475 290 1102 714.6 0 1102 291 1250 714.6 0 1250 292 1191 714.6 0 1191 293 619 714.6 0 619 294 522 714.6 0 522 295 588 714.6 0 588 296 751 714.6 0 751 297 892 714.6 0 892 298 1519 714.6 0 1519 299 2357 714.6 0 2357 300 4262 714.6 0 4262 301 28815 714.6 0 28815 Finished in 4.24 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 93,257 Reads with adapters: 4,799 (5.1%) Reads that were too short: 23 (0.0%) Reads written (passing filters): 4,776 (5.1%) Total basepairs processed: 20,613,685 bp Total written (filtered): 1,298,299 bp (6.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4799 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 84 0.0 3 0 0 0 0 84 18 5 0.0 3 0 0 4 0 1 19 55 0.0 3 0 54 0 0 1 20 4592 0.0 4 4515 75 1 0 1 21 15 0.0 4 0 14 0 0 1 22 12 0.0 4 2 1 1 0 8 23 2 0.0 4 0 0 0 1 1 31 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 1 111 2 0.0 4 0 0 1 1 114 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 1 142 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 191 2 0.0 4 0 0 0 1 1 195 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 248 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.17 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 4,776 Reads with adapters: 222 (4.6%) Reads written (passing filters): 4,776 (100.0%) Total basepairs processed: 1,298,299 bp Total written (filtered): 1,295,762 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 222 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.3% C: 0.5% G: 96.4% T: 0.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 74.6 0 1 4 2 18.7 0 2 6 29 1.2 0 29 9 3 0.0 0 0 3 10 94 0.0 1 4 90 11 91 0.0 1 0 91 114 1 0.0 1 0 1 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.