This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACTTCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 78.83 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 215,859 (7.4%) Reads that were too short: 100,989 (3.5%) Reads written (passing filters): 114,870 (3.9%) Total basepairs processed: 881,011,047 bp Total written (filtered): 22,529,644 bp (2.6%) === Adapter 1 === Sequence: ACTTCA; Type: regular 5'; Length: 6; Trimmed: 215859 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 416 714.6 0 416 7 21597 714.6 0 21597 8 174 714.6 0 174 9 6397 714.6 0 6397 10 79 714.6 0 79 11 36 714.6 0 36 12 27 714.6 0 27 13 26 714.6 0 26 14 38 714.6 0 38 15 88 714.6 0 88 16 46 714.6 0 46 17 39 714.6 0 39 18 48 714.6 0 48 19 43 714.6 0 43 20 36 714.6 0 36 21 23 714.6 0 23 22 37 714.6 0 37 23 39 714.6 0 39 24 31 714.6 0 31 25 41 714.6 0 41 26 39 714.6 0 39 27 25 714.6 0 25 28 46 714.6 0 46 29 34 714.6 0 34 30 37 714.6 0 37 31 27 714.6 0 27 32 31 714.6 0 31 33 39 714.6 0 39 34 43 714.6 0 43 35 41 714.6 0 41 36 36 714.6 0 36 37 53 714.6 0 53 38 49 714.6 0 49 39 42 714.6 0 42 40 34 714.6 0 34 41 50 714.6 0 50 42 22 714.6 0 22 43 31 714.6 0 31 44 25 714.6 0 25 45 30 714.6 0 30 46 28 714.6 0 28 47 42 714.6 0 42 48 43 714.6 0 43 49 31 714.6 0 31 50 31 714.6 0 31 51 34 714.6 0 34 52 42 714.6 0 42 53 35 714.6 0 35 54 37 714.6 0 37 55 35 714.6 0 35 56 59 714.6 0 59 57 525 714.6 0 525 58 434 714.6 0 434 59 113 714.6 0 113 60 26 714.6 0 26 61 36 714.6 0 36 62 45 714.6 0 45 63 48 714.6 0 48 64 50 714.6 0 50 65 30 714.6 0 30 66 30 714.6 0 30 67 31 714.6 0 31 68 44 714.6 0 44 69 31 714.6 0 31 70 30 714.6 0 30 71 47 714.6 0 47 72 171 714.6 0 171 73 124 714.6 0 124 74 252 714.6 0 252 75 205 714.6 0 205 76 181 714.6 0 181 77 152 714.6 0 152 78 177 714.6 0 177 79 317 714.6 0 317 80 279 714.6 0 279 81 190 714.6 0 190 82 388 714.6 0 388 83 410 714.6 0 410 84 559 714.6 0 559 85 495 714.6 0 495 86 463 714.6 0 463 87 385 714.6 0 385 88 1731 714.6 0 1731 89 1371 714.6 0 1371 90 1817 714.6 0 1817 91 1387 714.6 0 1387 92 1185 714.6 0 1185 93 225 714.6 0 225 94 303 714.6 0 303 95 354 714.6 0 354 96 299 714.6 0 299 97 193 714.6 0 193 98 276 714.6 0 276 99 358 714.6 0 358 100 352 714.6 0 352 101 532 714.6 0 532 102 520 714.6 0 520 103 376 714.6 0 376 104 570 714.6 0 570 105 416 714.6 0 416 106 226 714.6 0 226 107 231 714.6 0 231 108 566 714.6 0 566 109 621 714.6 0 621 110 374 714.6 0 374 111 334 714.6 0 334 112 358 714.6 0 358 113 369 714.6 0 369 114 321 714.6 0 321 115 622 714.6 0 622 116 636 714.6 0 636 117 395 714.6 0 395 118 201 714.6 0 201 119 184 714.6 0 184 120 277 714.6 0 277 121 694 714.6 0 694 122 1837 714.6 0 1837 123 1112 714.6 0 1112 124 570 714.6 0 570 125 410 714.6 0 410 126 369 714.6 0 369 127 481 714.6 0 481 128 457 714.6 0 457 129 412 714.6 0 412 130 735 714.6 0 735 131 784 714.6 0 784 132 467 714.6 0 467 133 312 714.6 0 312 134 480 714.6 0 480 135 617 714.6 0 617 136 739 714.6 0 739 137 636 714.6 0 636 138 526 714.6 0 526 139 366 714.6 0 366 140 694 714.6 0 694 141 2585 714.6 0 2585 142 2288 714.6 0 2288 143 1102 714.6 0 1102 144 380 714.6 0 380 145 426 714.6 0 426 146 610 714.6 0 610 147 957 714.6 0 957 148 1657 714.6 0 1657 149 782 714.6 0 782 150 647 714.6 0 647 151 1331 714.6 0 1331 152 1816 714.6 0 1816 153 1016 714.6 0 1016 154 808 714.6 0 808 155 764 714.6 0 764 156 718 714.6 0 718 157 1632 714.6 0 1632 158 1192 714.6 0 1192 159 723 714.6 0 723 160 713 714.6 0 713 161 871 714.6 0 871 162 1409 714.6 0 1409 163 858 714.6 0 858 164 470 714.6 0 470 165 415 714.6 0 415 166 755 714.6 0 755 167 919 714.6 0 919 168 722 714.6 0 722 169 668 714.6 0 668 170 669 714.6 0 669 171 427 714.6 0 427 172 287 714.6 0 287 173 223 714.6 0 223 174 370 714.6 0 370 175 824 714.6 0 824 176 858 714.6 0 858 177 566 714.6 0 566 178 437 714.6 0 437 179 395 714.6 0 395 180 377 714.6 0 377 181 423 714.6 0 423 182 1211 714.6 0 1211 183 1404 714.6 0 1404 184 866 714.6 0 866 185 603 714.6 0 603 186 720 714.6 0 720 187 994 714.6 0 994 188 761 714.6 0 761 189 484 714.6 0 484 190 359 714.6 0 359 191 425 714.6 0 425 192 416 714.6 0 416 193 498 714.6 0 498 194 380 714.6 0 380 195 525 714.6 0 525 196 683 714.6 0 683 197 587 714.6 0 587 198 608 714.6 0 608 199 386 714.6 0 386 200 254 714.6 0 254 201 258 714.6 0 258 202 560 714.6 0 560 203 783 714.6 0 783 204 697 714.6 0 697 205 689 714.6 0 689 206 637 714.6 0 637 207 650 714.6 0 650 208 1019 714.6 0 1019 209 2101 714.6 0 2101 210 1759 714.6 0 1759 211 1286 714.6 0 1286 212 1436 714.6 0 1436 213 13821 714.6 0 13821 214 10318 714.6 0 10318 215 4733 714.6 0 4733 216 779 714.6 0 779 217 657 714.6 0 657 218 677 714.6 0 677 219 455 714.6 0 455 220 471 714.6 0 471 221 1886 714.6 0 1886 222 3035 714.6 0 3035 223 2216 714.6 0 2216 224 795 714.6 0 795 225 527 714.6 0 527 226 741 714.6 0 741 227 708 714.6 0 708 228 548 714.6 0 548 229 484 714.6 0 484 230 357 714.6 0 357 231 539 714.6 0 539 232 609 714.6 0 609 233 729 714.6 0 729 234 782 714.6 0 782 235 917 714.6 0 917 236 588 714.6 0 588 237 747 714.6 0 747 238 387 714.6 0 387 239 352 714.6 0 352 240 410 714.6 0 410 241 290 714.6 0 290 242 620 714.6 0 620 243 1168 714.6 0 1168 244 2026 714.6 0 2026 245 1153 714.6 0 1153 246 624 714.6 0 624 247 424 714.6 0 424 248 279 714.6 0 279 249 153 714.6 0 153 250 139 714.6 0 139 251 346 714.6 0 346 252 719 714.6 0 719 253 977 714.6 0 977 254 869 714.6 0 869 255 977 714.6 0 977 256 1039 714.6 0 1039 257 2284 714.6 0 2284 258 2207 714.6 0 2207 259 746 714.6 0 746 260 234 714.6 0 234 261 299 714.6 0 299 262 430 714.6 0 430 263 782 714.6 0 782 264 1107 714.6 0 1107 265 923 714.6 0 923 266 692 714.6 0 692 267 663 714.6 0 663 268 408 714.6 0 408 269 248 714.6 0 248 270 429 714.6 0 429 271 881 714.6 0 881 272 784 714.6 0 784 273 1001 714.6 0 1001 274 589 714.6 0 589 275 341 714.6 0 341 276 266 714.6 0 266 277 407 714.6 0 407 278 690 714.6 0 690 279 1037 714.6 0 1037 280 545 714.6 0 545 281 461 714.6 0 461 282 565 714.6 0 565 283 538 714.6 0 538 284 1071 714.6 0 1071 285 1322 714.6 0 1322 286 820 714.6 0 820 287 342 714.6 0 342 288 179 714.6 0 179 289 200 714.6 0 200 290 276 714.6 0 276 291 715 714.6 0 715 292 719 714.6 0 719 293 443 714.6 0 443 294 381 714.6 0 381 295 383 714.6 0 383 296 253 714.6 0 253 297 297 714.6 0 297 298 366 714.6 0 366 299 415 714.6 0 415 300 290 714.6 0 290 301 272 714.6 0 272 Finished in 4.14 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 114,870 Reads with adapters: 6,691 (5.8%) Reads that were too short: 46 (0.0%) Reads written (passing filters): 6,645 (5.8%) Total basepairs processed: 22,529,644 bp Total written (filtered): 1,822,133 bp (8.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6691 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 10 0.0 3 0 0 6 4 16 67 0.0 3 0 61 4 2 17 6487 0.0 3 6253 201 23 10 18 25 0.0 3 3 20 2 19 9 0.0 3 0 1 4 4 20 7 0.0 3 0 0 0 7 21 1 0.0 3 0 0 0 1 26 1 0.0 3 1 27 1 0.0 3 1 31 3 0.0 3 0 0 0 3 32 1 0.0 3 0 0 0 1 33 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 37 2 0.0 3 0 0 0 2 38 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 45 5 0.0 3 0 0 0 5 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 75 3 0.0 3 0 0 0 3 77 1 0.0 3 0 0 0 1 83 3 0.0 3 0 0 0 3 84 3 0.0 3 0 0 0 3 85 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 107 3 0.0 3 0 0 1 2 108 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 3 0.0 3 0 0 0 3 124 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 0 2 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 160 2 0.0 3 0 0 0 2 166 2 0.0 3 0 0 0 2 167 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 3 0.0 3 0 0 0 3 283 1 0.0 3 0 0 0 1 288 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.23 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 6,645 Reads with adapters: 485 (7.3%) Reads written (passing filters): 6,645 (100.0%) Total basepairs processed: 1,822,133 bp Total written (filtered): 1,820,673 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 485 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 27.6% C: 10.9% G: 58.8% T: 2.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 480 103.8 0 480 4 5 26.0 0 5