This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 78.79 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 66,923 (2.3%) Reads that were too short: 24,398 (0.8%) Reads written (passing filters): 42,525 (1.5%) Total basepairs processed: 881,011,047 bp Total written (filtered): 8,986,326 bp (1.0%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 66923 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 175 714.6 0 175 7 2000 714.6 0 2000 8 6822 714.6 0 6822 9 79 714.6 0 79 10 35 714.6 0 35 11 41 714.6 0 41 12 43 714.6 0 43 13 37 714.6 0 37 14 48 714.6 0 48 15 36 714.6 0 36 16 34 714.6 0 34 17 50 714.6 0 50 18 55 714.6 0 55 19 49 714.6 0 49 20 38 714.6 0 38 21 64 714.6 0 64 22 68 714.6 0 68 23 414 714.6 0 414 24 48 714.6 0 48 25 39 714.6 0 39 26 37 714.6 0 37 27 41 714.6 0 41 28 42 714.6 0 42 29 52 714.6 0 52 30 68 714.6 0 68 31 275 714.6 0 275 32 51 714.6 0 51 33 79 714.6 0 79 34 51 714.6 0 51 35 73 714.6 0 73 36 36 714.6 0 36 37 41 714.6 0 41 38 50 714.6 0 50 39 77 714.6 0 77 40 59 714.6 0 59 41 77 714.6 0 77 42 40 714.6 0 40 43 80 714.6 0 80 44 51 714.6 0 51 45 47 714.6 0 47 46 50 714.6 0 50 47 43 714.6 0 43 48 28 714.6 0 28 49 43 714.6 0 43 50 42 714.6 0 42 51 52 714.6 0 52 52 56 714.6 0 56 53 49 714.6 0 49 54 36 714.6 0 36 55 58 714.6 0 58 56 47 714.6 0 47 57 104 714.6 0 104 58 48 714.6 0 48 59 54 714.6 0 54 60 57 714.6 0 57 61 54 714.6 0 54 62 40 714.6 0 40 63 62 714.6 0 62 64 92 714.6 0 92 65 9178 714.6 0 9178 66 341 714.6 0 341 67 43 714.6 0 43 68 38 714.6 0 38 69 46 714.6 0 46 70 57 714.6 0 57 71 54 714.6 0 54 72 40 714.6 0 40 73 46 714.6 0 46 74 43 714.6 0 43 75 50 714.6 0 50 76 47 714.6 0 47 77 37 714.6 0 37 78 43 714.6 0 43 79 48 714.6 0 48 80 37 714.6 0 37 81 67 714.6 0 67 82 69 714.6 0 69 83 87 714.6 0 87 84 82 714.6 0 82 85 125 714.6 0 125 86 171 714.6 0 171 87 70 714.6 0 70 88 45 714.6 0 45 89 36 714.6 0 36 90 52 714.6 0 52 91 61 714.6 0 61 92 79 714.6 0 79 93 75 714.6 0 75 94 84 714.6 0 84 95 115 714.6 0 115 96 138 714.6 0 138 97 99 714.6 0 99 98 134 714.6 0 134 99 168 714.6 0 168 100 167 714.6 0 167 101 208 714.6 0 208 102 250 714.6 0 250 103 285 714.6 0 285 104 169 714.6 0 169 105 85 714.6 0 85 106 48 714.6 0 48 107 64 714.6 0 64 108 50 714.6 0 50 109 40 714.6 0 40 110 100 714.6 0 100 111 142 714.6 0 142 112 120 714.6 0 120 113 148 714.6 0 148 114 97 714.6 0 97 115 94 714.6 0 94 116 138 714.6 0 138 117 140 714.6 0 140 118 148 714.6 0 148 119 117 714.6 0 117 120 80 714.6 0 80 121 66 714.6 0 66 122 91 714.6 0 91 123 209 714.6 0 209 124 246 714.6 0 246 125 239 714.6 0 239 126 100 714.6 0 100 127 119 714.6 0 119 128 96 714.6 0 96 129 114 714.6 0 114 130 121 714.6 0 121 131 151 714.6 0 151 132 217 714.6 0 217 133 270 714.6 0 270 134 165 714.6 0 165 135 195 714.6 0 195 136 124 714.6 0 124 137 183 714.6 0 183 138 289 714.6 0 289 139 301 714.6 0 301 140 153 714.6 0 153 141 142 714.6 0 142 142 264 714.6 0 264 143 400 714.6 0 400 144 483 714.6 0 483 145 444 714.6 0 444 146 137 714.6 0 137 147 126 714.6 0 126 148 120 714.6 0 120 149 133 714.6 0 133 150 133 714.6 0 133 151 166 714.6 0 166 152 117 714.6 0 117 153 128 714.6 0 128 154 102 714.6 0 102 155 85 714.6 0 85 156 106 714.6 0 106 157 132 714.6 0 132 158 234 714.6 0 234 159 201 714.6 0 201 160 190 714.6 0 190 161 108 714.6 0 108 162 171 714.6 0 171 163 119 714.6 0 119 164 135 714.6 0 135 165 115 714.6 0 115 166 120 714.6 0 120 167 204 714.6 0 204 168 222 714.6 0 222 169 234 714.6 0 234 170 309 714.6 0 309 171 365 714.6 0 365 172 311 714.6 0 311 173 158 714.6 0 158 174 88 714.6 0 88 175 89 714.6 0 89 176 123 714.6 0 123 177 264 714.6 0 264 178 269 714.6 0 269 179 212 714.6 0 212 180 233 714.6 0 233 181 125 714.6 0 125 182 123 714.6 0 123 183 182 714.6 0 182 184 292 714.6 0 292 185 239 714.6 0 239 186 172 714.6 0 172 187 381 714.6 0 381 188 283 714.6 0 283 189 267 714.6 0 267 190 144 714.6 0 144 191 123 714.6 0 123 192 145 714.6 0 145 193 150 714.6 0 150 194 251 714.6 0 251 195 172 714.6 0 172 196 184 714.6 0 184 197 190 714.6 0 190 198 350 714.6 0 350 199 309 714.6 0 309 200 121 714.6 0 121 201 69 714.6 0 69 202 84 714.6 0 84 203 88 714.6 0 88 204 169 714.6 0 169 205 165 714.6 0 165 206 203 714.6 0 203 207 188 714.6 0 188 208 132 714.6 0 132 209 104 714.6 0 104 210 221 714.6 0 221 211 380 714.6 0 380 212 280 714.6 0 280 213 196 714.6 0 196 214 637 714.6 0 637 215 732 714.6 0 732 216 747 714.6 0 747 217 176 714.6 0 176 218 165 714.6 0 165 219 202 714.6 0 202 220 140 714.6 0 140 221 104 714.6 0 104 222 101 714.6 0 101 223 664 714.6 0 664 224 685 714.6 0 685 225 438 714.6 0 438 226 167 714.6 0 167 227 165 714.6 0 165 228 212 714.6 0 212 229 182 714.6 0 182 230 128 714.6 0 128 231 103 714.6 0 103 232 74 714.6 0 74 233 94 714.6 0 94 234 102 714.6 0 102 235 135 714.6 0 135 236 147 714.6 0 147 237 180 714.6 0 180 238 254 714.6 0 254 239 224 714.6 0 224 240 335 714.6 0 335 241 125 714.6 0 125 242 76 714.6 0 76 243 87 714.6 0 87 244 88 714.6 0 88 245 169 714.6 0 169 246 314 714.6 0 314 247 512 714.6 0 512 248 231 714.6 0 231 249 72 714.6 0 72 250 49 714.6 0 49 251 47 714.6 0 47 252 56 714.6 0 56 253 107 714.6 0 107 254 150 714.6 0 150 255 160 714.6 0 160 256 210 714.6 0 210 257 282 714.6 0 282 258 300 714.6 0 300 259 181 714.6 0 181 260 147 714.6 0 147 261 82 714.6 0 82 262 107 714.6 0 107 263 269 714.6 0 269 264 340 714.6 0 340 265 570 714.6 0 570 266 174 714.6 0 174 267 268 714.6 0 268 268 195 714.6 0 195 269 137 714.6 0 137 270 62 714.6 0 62 271 153 714.6 0 153 272 1068 714.6 0 1068 273 1374 714.6 0 1374 274 1106 714.6 0 1106 275 168 714.6 0 168 276 155 714.6 0 155 277 182 714.6 0 182 278 217 714.6 0 217 279 208 714.6 0 208 280 143 714.6 0 143 281 90 714.6 0 90 282 56 714.6 0 56 283 151 714.6 0 151 284 192 714.6 0 192 285 303 714.6 0 303 286 380 714.6 0 380 287 468 714.6 0 468 288 189 714.6 0 189 289 67 714.6 0 67 290 45 714.6 0 45 291 59 714.6 0 59 292 129 714.6 0 129 293 271 714.6 0 271 294 220 714.6 0 220 295 127 714.6 0 127 296 81 714.6 0 81 297 58 714.6 0 58 298 74 714.6 0 74 299 190 714.6 0 190 300 408 714.6 0 408 301 996 714.6 0 996 Finished in 1.83 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 42,525 Reads with adapters: 7,035 (16.5%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 7,019 (16.5%) Total basepairs processed: 8,986,326 bp Total written (filtered): 1,914,473 bp (21.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 7035 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 3 19 61 0.0 3 0 59 1 0 1 20 6915 0.0 4 6845 68 2 21 17 0.0 4 3 13 1 22 2 0.0 4 0 0 1 0 1 23 2 0.0 4 0 0 0 2 25 1 0.0 4 0 0 0 0 1 28 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 1 48 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 56 1 0.0 4 1 69 1 0.0 4 0 0 0 0 1 70 3 0.0 4 0 0 0 0 3 77 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 103 2 0.0 4 0 0 0 0 2 108 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 1 113 1 0.0 4 0 0 0 1 133 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 177 2 0.0 4 0 0 0 0 2 199 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.24 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 7,019 Reads with adapters: 347 (4.9%) Reads written (passing filters): 7,019 (100.0%) Total basepairs processed: 1,914,473 bp Total written (filtered): 1,900,196 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 347 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 9.8% C: 5.2% G: 83.6% T: 1.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 6 1 1.7 0 1 10 27 0.0 1 0 27 11 273 0.0 1 1 272 12 1 0.0 1 0 1 13 1 0.0 1 0 1 224 11 0.0 1 8 3 225 2 0.0 1 2 259 1 0.0 1 1 260 30 0.0 1 30 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.