This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.42 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 279,179 (9.5%) Reads that were too short: 17,940 (0.6%) Reads written (passing filters): 261,239 (8.9%) Total basepairs processed: 881,011,047 bp Total written (filtered): 59,875,861 bp (6.8%) === Adapter 1 === Sequence: TCCAGC; Type: regular 5'; Length: 6; Trimmed: 279179 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 869 714.6 0 869 7 1669 714.6 0 1669 8 60311 714.6 0 60311 9 4279 714.6 0 4279 10 68 714.6 0 68 11 49 714.6 0 49 12 71 714.6 0 71 13 53 714.6 0 53 14 59 714.6 0 59 15 60 714.6 0 60 16 53 714.6 0 53 17 66 714.6 0 66 18 79 714.6 0 79 19 56 714.6 0 56 20 77 714.6 0 77 21 65 714.6 0 65 22 71 714.6 0 71 23 92 714.6 0 92 24 83 714.6 0 83 25 74 714.6 0 74 26 80 714.6 0 80 27 62 714.6 0 62 28 85 714.6 0 85 29 81 714.6 0 81 30 79 714.6 0 79 31 91 714.6 0 91 32 88 714.6 0 88 33 61 714.6 0 61 34 101 714.6 0 101 35 86 714.6 0 86 36 123 714.6 0 123 37 98 714.6 0 98 38 148 714.6 0 148 39 83 714.6 0 83 40 90 714.6 0 90 41 78 714.6 0 78 42 114 714.6 0 114 43 125 714.6 0 125 44 117 714.6 0 117 45 169 714.6 0 169 46 218 714.6 0 218 47 317 714.6 0 317 48 630 714.6 0 630 49 1638 714.6 0 1638 50 387 714.6 0 387 51 585 714.6 0 585 52 1189 714.6 0 1189 53 554 714.6 0 554 54 455 714.6 0 455 55 701 714.6 0 701 56 738 714.6 0 738 57 771 714.6 0 771 58 792 714.6 0 792 59 1123 714.6 0 1123 60 1110 714.6 0 1110 61 1809 714.6 0 1809 62 32968 714.6 0 32968 63 29786 714.6 0 29786 64 47867 714.6 0 47867 65 531 714.6 0 531 66 64 714.6 0 64 67 61 714.6 0 61 68 60 714.6 0 60 69 64 714.6 0 64 70 65 714.6 0 65 71 90 714.6 0 90 72 162 714.6 0 162 73 202 714.6 0 202 74 88 714.6 0 88 75 93 714.6 0 93 76 834 714.6 0 834 77 1001 714.6 0 1001 78 356 714.6 0 356 79 69 714.6 0 69 80 75 714.6 0 75 81 88 714.6 0 88 82 81 714.6 0 81 83 117 714.6 0 117 84 107 714.6 0 107 85 182 714.6 0 182 86 106 714.6 0 106 87 126 714.6 0 126 88 74 714.6 0 74 89 90 714.6 0 90 90 85 714.6 0 85 91 97 714.6 0 97 92 108 714.6 0 108 93 268 714.6 0 268 94 186 714.6 0 186 95 81 714.6 0 81 96 49 714.6 0 49 97 65 714.6 0 65 98 57 714.6 0 57 99 52 714.6 0 52 100 55 714.6 0 55 101 106 714.6 0 106 102 131 714.6 0 131 103 78 714.6 0 78 104 89 714.6 0 89 105 193 714.6 0 193 106 111 714.6 0 111 107 176 714.6 0 176 108 216 714.6 0 216 109 110 714.6 0 110 110 67 714.6 0 67 111 123 714.6 0 123 112 104 714.6 0 104 113 62 714.6 0 62 114 85 714.6 0 85 115 99 714.6 0 99 116 104 714.6 0 104 117 101 714.6 0 101 118 98 714.6 0 98 119 88 714.6 0 88 120 93 714.6 0 93 121 71 714.6 0 71 122 80 714.6 0 80 123 97 714.6 0 97 124 115 714.6 0 115 125 119 714.6 0 119 126 134 714.6 0 134 127 150 714.6 0 150 128 130 714.6 0 130 129 122 714.6 0 122 130 88 714.6 0 88 131 98 714.6 0 98 132 131 714.6 0 131 133 154 714.6 0 154 134 162 714.6 0 162 135 125 714.6 0 125 136 111 714.6 0 111 137 136 714.6 0 136 138 134 714.6 0 134 139 113 714.6 0 113 140 165 714.6 0 165 141 156 714.6 0 156 142 250 714.6 0 250 143 276 714.6 0 276 144 2154 714.6 0 2154 145 1834 714.6 0 1834 146 1439 714.6 0 1439 147 191 714.6 0 191 148 102 714.6 0 102 149 237 714.6 0 237 150 277 714.6 0 277 151 201 714.6 0 201 152 157 714.6 0 157 153 160 714.6 0 160 154 181 714.6 0 181 155 270 714.6 0 270 156 1245 714.6 0 1245 157 1194 714.6 0 1194 158 518 714.6 0 518 159 288 714.6 0 288 160 298 714.6 0 298 161 390 714.6 0 390 162 532 714.6 0 532 163 1050 714.6 0 1050 164 22483 714.6 0 22483 165 5518 714.6 0 5518 166 2535 714.6 0 2535 167 354 714.6 0 354 168 261 714.6 0 261 169 692 714.6 0 692 170 965 714.6 0 965 171 1000 714.6 0 1000 172 671 714.6 0 671 173 523 714.6 0 523 174 251 714.6 0 251 175 151 714.6 0 151 176 111 714.6 0 111 177 178 714.6 0 178 178 175 714.6 0 175 179 334 714.6 0 334 180 269 714.6 0 269 181 360 714.6 0 360 182 255 714.6 0 255 183 174 714.6 0 174 184 247 714.6 0 247 185 305 714.6 0 305 186 339 714.6 0 339 187 181 714.6 0 181 188 204 714.6 0 204 189 842 714.6 0 842 190 1239 714.6 0 1239 191 1284 714.6 0 1284 192 425 714.6 0 425 193 123 714.6 0 123 194 84 714.6 0 84 195 134 714.6 0 134 196 122 714.6 0 122 197 162 714.6 0 162 198 160 714.6 0 160 199 129 714.6 0 129 200 118 714.6 0 118 201 122 714.6 0 122 202 94 714.6 0 94 203 128 714.6 0 128 204 210 714.6 0 210 205 139 714.6 0 139 206 190 714.6 0 190 207 202 714.6 0 202 208 185 714.6 0 185 209 128 714.6 0 128 210 114 714.6 0 114 211 116 714.6 0 116 212 172 714.6 0 172 213 192 714.6 0 192 214 181 714.6 0 181 215 164 714.6 0 164 216 380 714.6 0 380 217 425 714.6 0 425 218 400 714.6 0 400 219 167 714.6 0 167 220 98 714.6 0 98 221 147 714.6 0 147 222 94 714.6 0 94 223 97 714.6 0 97 224 130 714.6 0 130 225 228 714.6 0 228 226 321 714.6 0 321 227 301 714.6 0 301 228 342 714.6 0 342 229 380 714.6 0 380 230 435 714.6 0 435 231 158 714.6 0 158 232 126 714.6 0 126 233 145 714.6 0 145 234 127 714.6 0 127 235 105 714.6 0 105 236 158 714.6 0 158 237 191 714.6 0 191 238 130 714.6 0 130 239 115 714.6 0 115 240 96 714.6 0 96 241 95 714.6 0 95 242 55 714.6 0 55 243 90 714.6 0 90 244 121 714.6 0 121 245 222 714.6 0 222 246 194 714.6 0 194 247 189 714.6 0 189 248 163 714.6 0 163 249 99 714.6 0 99 250 123 714.6 0 123 251 79 714.6 0 79 252 88 714.6 0 88 253 75 714.6 0 75 254 165 714.6 0 165 255 174 714.6 0 174 256 165 714.6 0 165 257 159 714.6 0 159 258 106 714.6 0 106 259 152 714.6 0 152 260 547 714.6 0 547 261 569 714.6 0 569 262 244 714.6 0 244 263 117 714.6 0 117 264 129 714.6 0 129 265 153 714.6 0 153 266 219 714.6 0 219 267 280 714.6 0 280 268 207 714.6 0 207 269 120 714.6 0 120 270 116 714.6 0 116 271 110 714.6 0 110 272 137 714.6 0 137 273 94 714.6 0 94 274 73 714.6 0 73 275 115 714.6 0 115 276 181 714.6 0 181 277 170 714.6 0 170 278 124 714.6 0 124 279 93 714.6 0 93 280 171 714.6 0 171 281 230 714.6 0 230 282 112 714.6 0 112 283 76 714.6 0 76 284 145 714.6 0 145 285 145 714.6 0 145 286 163 714.6 0 163 287 670 714.6 0 670 288 925 714.6 0 925 289 282 714.6 0 282 290 92 714.6 0 92 291 59 714.6 0 59 292 54 714.6 0 54 293 108 714.6 0 108 294 178 714.6 0 178 295 140 714.6 0 140 296 150 714.6 0 150 297 131 714.6 0 131 298 106 714.6 0 106 299 59 714.6 0 59 300 52 714.6 0 52 301 69 714.6 0 69 Finished in 10.36 s (40 us/read; 1.51 M reads/minute). === Summary === Total reads processed: 261,239 Reads with adapters: 62,800 (24.0%) Reads that were too short: 322 (0.1%) Reads written (passing filters): 62,478 (23.9%) Total basepairs processed: 59,875,861 bp Total written (filtered): 17,154,039 bp (28.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 62800 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 24 0.0 3 0 0 15 9 16 474 0.0 3 0 421 34 19 17 61091 0.0 3 58418 2270 316 87 18 144 0.0 3 7 115 9 13 19 126 0.0 3 0 1 67 58 20 43 0.0 3 0 0 0 43 26 1 0.0 3 0 0 0 1 28 4 0.0 3 0 0 0 4 29 2 0.0 3 0 0 0 2 32 1 0.0 3 1 34 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 8 0.0 3 0 0 0 8 40 2 0.0 3 0 0 0 2 41 3 0.0 3 0 0 0 3 47 9 0.0 3 0 0 0 9 49 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 3 0.0 3 0 0 0 3 62 1 0.0 3 0 0 0 1 64 4 0.0 3 0 0 0 4 65 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 89 3 0.0 3 0 0 0 3 90 3 0.0 3 0 0 0 3 91 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 104 6 0.0 3 0 0 0 6 105 6 0.0 3 0 0 0 6 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 25 0.0 3 0 0 0 25 110 1 0.0 3 0 0 0 1 112 6 0.0 3 0 0 0 6 114 6 0.0 3 0 0 0 6 115 1 0.0 3 0 0 0 1 116 3 0.0 3 0 0 0 3 117 5 0.0 3 0 0 0 5 118 1 0.0 3 0 0 0 1 123 5 0.0 3 0 0 0 5 124 3 0.0 3 0 0 0 3 125 1 0.0 3 0 0 0 1 127 5 0.0 3 0 0 0 5 128 11 0.0 3 0 0 0 11 129 357 0.0 3 0 0 1 356 130 3 0.0 3 0 0 0 3 131 14 0.0 3 0 0 0 14 132 85 0.0 3 0 0 1 84 133 2 0.0 3 0 0 0 2 135 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 153 5 0.0 3 0 0 1 4 155 11 0.0 3 0 0 2 9 156 2 0.0 3 0 0 0 2 164 2 0.0 3 0 0 0 2 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 1 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 180 3 0.0 3 0 0 0 3 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 189 20 0.0 3 0 0 0 20 192 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 205 3 0.0 3 0 0 0 3 206 8 0.0 3 0 0 0 8 207 1 0.0 3 0 0 1 215 2 0.0 3 0 0 0 2 216 2 0.0 3 0 0 0 2 218 2 0.0 3 0 0 0 2 220 4 0.0 3 0 0 0 4 221 4 0.0 3 0 0 0 4 230 14 0.0 3 0 0 0 14 231 160 0.0 3 0 0 5 155 232 5 0.0 3 0 0 0 5 234 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.17 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 62,478 Reads with adapters: 701 (1.1%) Reads written (passing filters): 62,478 (100.0%) Total basepairs processed: 17,154,039 bp Total written (filtered): 17,151,911 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 701 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 59.1% C: 19.5% G: 15.8% T: 5.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 681 976.2 0 681 4 15 244.1 0 15 5 5 61.0 0 5