This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGCAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.95 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 253,688 (8.7%) Reads that were too short: 69,017 (2.4%) Reads written (passing filters): 184,671 (6.3%) Total basepairs processed: 881,011,047 bp Total written (filtered): 39,102,650 bp (4.4%) === Adapter 1 === Sequence: AAGCAG; Type: regular 5'; Length: 6; Trimmed: 253688 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2082 714.6 0 2082 7 63402 714.6 0 63402 8 284 714.6 0 284 9 3429 714.6 0 3429 10 87 714.6 0 87 11 126 714.6 0 126 12 91 714.6 0 91 13 99 714.6 0 99 14 119 714.6 0 119 15 104 714.6 0 104 16 92 714.6 0 92 17 122 714.6 0 122 18 82 714.6 0 82 19 91 714.6 0 91 20 113 714.6 0 113 21 112 714.6 0 112 22 122 714.6 0 122 23 107 714.6 0 107 24 96 714.6 0 96 25 196 714.6 0 196 26 236 714.6 0 236 27 94 714.6 0 94 28 89 714.6 0 89 29 101 714.6 0 101 30 100 714.6 0 100 31 65 714.6 0 65 32 103 714.6 0 103 33 106 714.6 0 106 34 78 714.6 0 78 35 90 714.6 0 90 36 118 714.6 0 118 37 87 714.6 0 87 38 103 714.6 0 103 39 93 714.6 0 93 40 71 714.6 0 71 41 115 714.6 0 115 42 87 714.6 0 87 43 82 714.6 0 82 44 116 714.6 0 116 45 96 714.6 0 96 46 91 714.6 0 91 47 82 714.6 0 82 48 83 714.6 0 83 49 87 714.6 0 87 50 103 714.6 0 103 51 148 714.6 0 148 52 96 714.6 0 96 53 122 714.6 0 122 54 76 714.6 0 76 55 85 714.6 0 85 56 105 714.6 0 105 57 82 714.6 0 82 58 95 714.6 0 95 59 101 714.6 0 101 60 105 714.6 0 105 61 242 714.6 0 242 62 113 714.6 0 113 63 308 714.6 0 308 64 67 714.6 0 67 65 93 714.6 0 93 66 96 714.6 0 96 67 91 714.6 0 91 68 79 714.6 0 79 69 110 714.6 0 110 70 118 714.6 0 118 71 205 714.6 0 205 72 94 714.6 0 94 73 70 714.6 0 70 74 123 714.6 0 123 75 98 714.6 0 98 76 103 714.6 0 103 77 193 714.6 0 193 78 359 714.6 0 359 79 227 714.6 0 227 80 412 714.6 0 412 81 563 714.6 0 563 82 767 714.6 0 767 83 1352 714.6 0 1352 84 629 714.6 0 629 85 273 714.6 0 273 86 187 714.6 0 187 87 159 714.6 0 159 88 176 714.6 0 176 89 195 714.6 0 195 90 137 714.6 0 137 91 311 714.6 0 311 92 417 714.6 0 417 93 381 714.6 0 381 94 668 714.6 0 668 95 455 714.6 0 455 96 280 714.6 0 280 97 471 714.6 0 471 98 336 714.6 0 336 99 460 714.6 0 460 100 453 714.6 0 453 101 504 714.6 0 504 102 398 714.6 0 398 103 239 714.6 0 239 104 194 714.6 0 194 105 280 714.6 0 280 106 250 714.6 0 250 107 261 714.6 0 261 108 328 714.6 0 328 109 512 714.6 0 512 110 545 714.6 0 545 111 515 714.6 0 515 112 3971 714.6 0 3971 113 5673 714.6 0 5673 114 9021 714.6 0 9021 115 420 714.6 0 420 116 473 714.6 0 473 117 665 714.6 0 665 118 792 714.6 0 792 119 1019 714.6 0 1019 120 1082 714.6 0 1082 121 1447 714.6 0 1447 122 1718 714.6 0 1718 123 477 714.6 0 477 124 500 714.6 0 500 125 672 714.6 0 672 126 557 714.6 0 557 127 864 714.6 0 864 128 522 714.6 0 522 129 491 714.6 0 491 130 512 714.6 0 512 131 653 714.6 0 653 132 553 714.6 0 553 133 510 714.6 0 510 134 605 714.6 0 605 135 607 714.6 0 607 136 1016 714.6 0 1016 137 636 714.6 0 636 138 590 714.6 0 590 139 798 714.6 0 798 140 622 714.6 0 622 141 570 714.6 0 570 142 845 714.6 0 845 143 1080 714.6 0 1080 144 928 714.6 0 928 145 680 714.6 0 680 146 631 714.6 0 631 147 696 714.6 0 696 148 744 714.6 0 744 149 755 714.6 0 755 150 579 714.6 0 579 151 690 714.6 0 690 152 767 714.6 0 767 153 606 714.6 0 606 154 745 714.6 0 745 155 673 714.6 0 673 156 566 714.6 0 566 157 697 714.6 0 697 158 601 714.6 0 601 159 596 714.6 0 596 160 555 714.6 0 555 161 691 714.6 0 691 162 579 714.6 0 579 163 664 714.6 0 664 164 741 714.6 0 741 165 669 714.6 0 669 166 1076 714.6 0 1076 167 1027 714.6 0 1027 168 847 714.6 0 847 169 1030 714.6 0 1030 170 911 714.6 0 911 171 1542 714.6 0 1542 172 2180 714.6 0 2180 173 1670 714.6 0 1670 174 932 714.6 0 932 175 982 714.6 0 982 176 750 714.6 0 750 177 853 714.6 0 853 178 778 714.6 0 778 179 764 714.6 0 764 180 856 714.6 0 856 181 798 714.6 0 798 182 819 714.6 0 819 183 1982 714.6 0 1982 184 2345 714.6 0 2345 185 1279 714.6 0 1279 186 692 714.6 0 692 187 726 714.6 0 726 188 741 714.6 0 741 189 642 714.6 0 642 190 848 714.6 0 848 191 1949 714.6 0 1949 192 1661 714.6 0 1661 193 1378 714.6 0 1378 194 1104 714.6 0 1104 195 803 714.6 0 803 196 691 714.6 0 691 197 997 714.6 0 997 198 867 714.6 0 867 199 1596 714.6 0 1596 200 783 714.6 0 783 201 614 714.6 0 614 202 654 714.6 0 654 203 833 714.6 0 833 204 665 714.6 0 665 205 623 714.6 0 623 206 609 714.6 0 609 207 594 714.6 0 594 208 616 714.6 0 616 209 584 714.6 0 584 210 609 714.6 0 609 211 756 714.6 0 756 212 669 714.6 0 669 213 663 714.6 0 663 214 641 714.6 0 641 215 816 714.6 0 816 216 917 714.6 0 917 217 694 714.6 0 694 218 792 714.6 0 792 219 808 714.6 0 808 220 756 714.6 0 756 221 538 714.6 0 538 222 527 714.6 0 527 223 762 714.6 0 762 224 716 714.6 0 716 225 733 714.6 0 733 226 619 714.6 0 619 227 657 714.6 0 657 228 835 714.6 0 835 229 796 714.6 0 796 230 673 714.6 0 673 231 583 714.6 0 583 232 630 714.6 0 630 233 590 714.6 0 590 234 648 714.6 0 648 235 858 714.6 0 858 236 762 714.6 0 762 237 632 714.6 0 632 238 573 714.6 0 573 239 657 714.6 0 657 240 612 714.6 0 612 241 472 714.6 0 472 242 517 714.6 0 517 243 754 714.6 0 754 244 649 714.6 0 649 245 738 714.6 0 738 246 652 714.6 0 652 247 655 714.6 0 655 248 356 714.6 0 356 249 361 714.6 0 361 250 327 714.6 0 327 251 725 714.6 0 725 252 1737 714.6 0 1737 253 992 714.6 0 992 254 622 714.6 0 622 255 736 714.6 0 736 256 733 714.6 0 733 257 697 714.6 0 697 258 557 714.6 0 557 259 574 714.6 0 574 260 441 714.6 0 441 261 507 714.6 0 507 262 684 714.6 0 684 263 717 714.6 0 717 264 652 714.6 0 652 265 778 714.6 0 778 266 517 714.6 0 517 267 935 714.6 0 935 268 1481 714.6 0 1481 269 872 714.6 0 872 270 523 714.6 0 523 271 644 714.6 0 644 272 793 714.6 0 793 273 640 714.6 0 640 274 577 714.6 0 577 275 493 714.6 0 493 276 432 714.6 0 432 277 495 714.6 0 495 278 667 714.6 0 667 279 565 714.6 0 565 280 850 714.6 0 850 281 573 714.6 0 573 282 492 714.6 0 492 283 1013 714.6 0 1013 284 879 714.6 0 879 285 529 714.6 0 529 286 600 714.6 0 600 287 645 714.6 0 645 288 524 714.6 0 524 289 512 714.6 0 512 290 817 714.6 0 817 291 1060 714.6 0 1060 292 1036 714.6 0 1036 293 1027 714.6 0 1027 294 594 714.6 0 594 295 493 714.6 0 493 296 686 714.6 0 686 297 826 714.6 0 826 298 960 714.6 0 960 299 882 714.6 0 882 300 910 714.6 0 910 301 142 714.6 0 142 Finished in 7.71 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 184,671 Reads with adapters: 65,274 (35.3%) Reads that were too short: 50 (0.0%) Reads written (passing filters): 65,224 (35.3%) Total basepairs processed: 39,102,650 bp Total written (filtered): 17,870,948 bp (45.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 65274 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 35 0.0 3 0 0 0 2 33 18 16 0.0 3 0 0 15 0 1 19 551 0.0 3 0 536 10 4 1 20 64391 0.0 4 63553 812 24 2 21 170 0.0 4 29 140 0 1 22 18 0.0 4 2 0 6 1 9 23 21 0.0 4 0 0 0 20 1 24 1 0.0 4 0 0 0 0 1 27 1 0.0 4 0 0 0 1 41 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 1 46 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 51 3 0.0 4 0 0 0 1 2 52 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 61 2 0.0 4 0 0 0 0 2 63 2 0.0 4 0 0 0 0 2 66 1 0.0 4 0 0 0 0 1 67 2 0.0 4 0 0 0 0 2 69 2 0.0 4 0 0 0 0 2 73 2 0.0 4 0 0 0 0 2 75 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 95 2 0.0 4 0 0 0 0 2 96 1 0.0 4 0 0 0 0 1 97 3 0.0 4 0 0 0 0 3 98 2 0.0 4 0 0 0 0 2 103 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 108 2 0.0 4 0 0 0 0 2 109 2 0.0 4 0 0 0 1 1 110 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 120 2 0.0 4 0 0 0 0 2 125 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 5 0.0 4 0 0 0 0 5 128 2 0.0 4 0 0 0 1 1 161 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 208 2 0.0 4 0 0 0 0 2 213 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.25 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 65,224 Reads with adapters: 3,779 (5.8%) Reads written (passing filters): 65,224 (100.0%) Total basepairs processed: 17,870,948 bp Total written (filtered): 17,827,895 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3779 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.9% C: 0.9% G: 97.9% T: 0.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 16 1019.1 0 16 4 2 254.8 0 2 5 1 63.7 0 1 10 4 0.1 1 0 4 11 3733 0.0 1 7 3726 12 16 0.0 1 0 16 210 3 0.0 1 1 2 265 3 0.0 1 3 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.