This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTTGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.52 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 55,039 (1.9%) Reads that were too short: 6,642 (0.2%) Reads written (passing filters): 48,397 (1.7%) Total basepairs processed: 881,011,047 bp Total written (filtered): 8,943,739 bp (1.0%) === Adapter 1 === Sequence: CTTGGT; Type: regular 5'; Length: 6; Trimmed: 55039 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1029 714.6 0 1029 7 2512 714.6 0 2512 8 4222 714.6 0 4222 9 68 714.6 0 68 10 45 714.6 0 45 11 36 714.6 0 36 12 35 714.6 0 35 13 37 714.6 0 37 14 39 714.6 0 39 15 54 714.6 0 54 16 48 714.6 0 48 17 38 714.6 0 38 18 44 714.6 0 44 19 74 714.6 0 74 20 79 714.6 0 79 21 80 714.6 0 80 22 43 714.6 0 43 23 51 714.6 0 51 24 45 714.6 0 45 25 30 714.6 0 30 26 55 714.6 0 55 27 76 714.6 0 76 28 69 714.6 0 69 29 69 714.6 0 69 30 56 714.6 0 56 31 38 714.6 0 38 32 53 714.6 0 53 33 36 714.6 0 36 34 50 714.6 0 50 35 53 714.6 0 53 36 59 714.6 0 59 37 52 714.6 0 52 38 52 714.6 0 52 39 94 714.6 0 94 40 333 714.6 0 333 41 3693 714.6 0 3693 42 85 714.6 0 85 43 43 714.6 0 43 44 50 714.6 0 50 45 57 714.6 0 57 46 41 714.6 0 41 47 54 714.6 0 54 48 37 714.6 0 37 49 44 714.6 0 44 50 49 714.6 0 49 51 39 714.6 0 39 52 52 714.6 0 52 53 47 714.6 0 47 54 51 714.6 0 51 55 57 714.6 0 57 56 54 714.6 0 54 57 59 714.6 0 59 58 44 714.6 0 44 59 46 714.6 0 46 60 49 714.6 0 49 61 29 714.6 0 29 62 44 714.6 0 44 63 47 714.6 0 47 64 47 714.6 0 47 65 56 714.6 0 56 66 45 714.6 0 45 67 47 714.6 0 47 68 62 714.6 0 62 69 47 714.6 0 47 70 43 714.6 0 43 71 63 714.6 0 63 72 50 714.6 0 50 73 72 714.6 0 72 74 60 714.6 0 60 75 53 714.6 0 53 76 133 714.6 0 133 77 223 714.6 0 223 78 142 714.6 0 142 79 51 714.6 0 51 80 52 714.6 0 52 81 61 714.6 0 61 82 54 714.6 0 54 83 50 714.6 0 50 84 58 714.6 0 58 85 73 714.6 0 73 86 68 714.6 0 68 87 55 714.6 0 55 88 58 714.6 0 58 89 41 714.6 0 41 90 48 714.6 0 48 91 81 714.6 0 81 92 85 714.6 0 85 93 205 714.6 0 205 94 239 714.6 0 239 95 140 714.6 0 140 96 246 714.6 0 246 97 456 714.6 0 456 98 382 714.6 0 382 99 88 714.6 0 88 100 41 714.6 0 41 101 42 714.6 0 42 102 63 714.6 0 63 103 48 714.6 0 48 104 37 714.6 0 37 105 30 714.6 0 30 106 35 714.6 0 35 107 40 714.6 0 40 108 51 714.6 0 51 109 38 714.6 0 38 110 52 714.6 0 52 111 51 714.6 0 51 112 36 714.6 0 36 113 39 714.6 0 39 114 83 714.6 0 83 115 87 714.6 0 87 116 73 714.6 0 73 117 54 714.6 0 54 118 38 714.6 0 38 119 33 714.6 0 33 120 38 714.6 0 38 121 39 714.6 0 39 122 42 714.6 0 42 123 48 714.6 0 48 124 41 714.6 0 41 125 36 714.6 0 36 126 56 714.6 0 56 127 43 714.6 0 43 128 100 714.6 0 100 129 62 714.6 0 62 130 278 714.6 0 278 131 203 714.6 0 203 132 177 714.6 0 177 133 37 714.6 0 37 134 56 714.6 0 56 135 61 714.6 0 61 136 47 714.6 0 47 137 58 714.6 0 58 138 36 714.6 0 36 139 39 714.6 0 39 140 54 714.6 0 54 141 53 714.6 0 53 142 56 714.6 0 56 143 66 714.6 0 66 144 117 714.6 0 117 145 48 714.6 0 48 146 38 714.6 0 38 147 35 714.6 0 35 148 33 714.6 0 33 149 47 714.6 0 47 150 37 714.6 0 37 151 68 714.6 0 68 152 105 714.6 0 105 153 45 714.6 0 45 154 61 714.6 0 61 155 52 714.6 0 52 156 52 714.6 0 52 157 56 714.6 0 56 158 61 714.6 0 61 159 47 714.6 0 47 160 35 714.6 0 35 161 48 714.6 0 48 162 93 714.6 0 93 163 87 714.6 0 87 164 155 714.6 0 155 165 136 714.6 0 136 166 222 714.6 0 222 167 329 714.6 0 329 168 801 714.6 0 801 169 19925 714.6 0 19925 170 2207 714.6 0 2207 171 1032 714.6 0 1032 172 47 714.6 0 47 173 46 714.6 0 46 174 60 714.6 0 60 175 40 714.6 0 40 176 40 714.6 0 40 177 32 714.6 0 32 178 39 714.6 0 39 179 55 714.6 0 55 180 54 714.6 0 54 181 37 714.6 0 37 182 55 714.6 0 55 183 47 714.6 0 47 184 56 714.6 0 56 185 43 714.6 0 43 186 47 714.6 0 47 187 32 714.6 0 32 188 44 714.6 0 44 189 41 714.6 0 41 190 39 714.6 0 39 191 39 714.6 0 39 192 87 714.6 0 87 193 135 714.6 0 135 194 81 714.6 0 81 195 49 714.6 0 49 196 43 714.6 0 43 197 34 714.6 0 34 198 39 714.6 0 39 199 31 714.6 0 31 200 45 714.6 0 45 201 32 714.6 0 32 202 58 714.6 0 58 203 113 714.6 0 113 204 91 714.6 0 91 205 72 714.6 0 72 206 64 714.6 0 64 207 36 714.6 0 36 208 56 714.6 0 56 209 37 714.6 0 37 210 41 714.6 0 41 211 67 714.6 0 67 212 68 714.6 0 68 213 58 714.6 0 58 214 46 714.6 0 46 215 49 714.6 0 49 216 69 714.6 0 69 217 51 714.6 0 51 218 57 714.6 0 57 219 38 714.6 0 38 220 40 714.6 0 40 221 41 714.6 0 41 222 63 714.6 0 63 223 65 714.6 0 65 224 74 714.6 0 74 225 48 714.6 0 48 226 42 714.6 0 42 227 50 714.6 0 50 228 63 714.6 0 63 229 45 714.6 0 45 230 50 714.6 0 50 231 83 714.6 0 83 232 64 714.6 0 64 233 64 714.6 0 64 234 45 714.6 0 45 235 66 714.6 0 66 236 96 714.6 0 96 237 133 714.6 0 133 238 130 714.6 0 130 239 66 714.6 0 66 240 42 714.6 0 42 241 120 714.6 0 120 242 37 714.6 0 37 243 46 714.6 0 46 244 46 714.6 0 46 245 38 714.6 0 38 246 57 714.6 0 57 247 23 714.6 0 23 248 19 714.6 0 19 249 41 714.6 0 41 250 23 714.6 0 23 251 31 714.6 0 31 252 32 714.6 0 32 253 43 714.6 0 43 254 62 714.6 0 62 255 61 714.6 0 61 256 98 714.6 0 98 257 47 714.6 0 47 258 85 714.6 0 85 259 32 714.6 0 32 260 36 714.6 0 36 261 63 714.6 0 63 262 45 714.6 0 45 263 41 714.6 0 41 264 35 714.6 0 35 265 62 714.6 0 62 266 40 714.6 0 40 267 52 714.6 0 52 268 41 714.6 0 41 269 49 714.6 0 49 270 56 714.6 0 56 271 212 714.6 0 212 272 400 714.6 0 400 273 309 714.6 0 309 274 856 714.6 0 856 275 33 714.6 0 33 276 28 714.6 0 28 277 41 714.6 0 41 278 34 714.6 0 34 279 38 714.6 0 38 280 29 714.6 0 29 281 34 714.6 0 34 282 36 714.6 0 36 283 42 714.6 0 42 284 31 714.6 0 31 285 31 714.6 0 31 286 36 714.6 0 36 287 28 714.6 0 28 288 37 714.6 0 37 289 48 714.6 0 48 290 51 714.6 0 51 291 36 714.6 0 36 292 47 714.6 0 47 293 47 714.6 0 47 294 46 714.6 0 46 295 25 714.6 0 25 296 36 714.6 0 36 297 47 714.6 0 47 298 33 714.6 0 33 299 21 714.6 0 21 300 24 714.6 0 24 301 24 714.6 0 24 Finished in 1.73 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 48,397 Reads with adapters: 3,236 (6.7%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 3,219 (6.7%) Total basepairs processed: 8,943,739 bp Total written (filtered): 887,969 bp (9.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3236 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 18 0.0 3 0 16 1 1 17 2405 0.0 3 2342 56 6 1 18 4 0.0 3 0 3 1 19 14 0.0 3 0 11 2 1 20 756 0.0 3 730 16 6 4 21 1 0.0 3 0 1 22 1 0.0 3 0 0 1 23 3 0.0 3 0 0 0 3 31 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 81 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 8 0.0 3 0 0 0 8 129 3 0.0 3 0 0 0 3 137 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.12 s (37 us/read; 1.61 M reads/minute). === Summary === Total reads processed: 3,219 Reads with adapters: 192 (6.0%) Reads written (passing filters): 3,219 (100.0%) Total basepairs processed: 887,969 bp Total written (filtered): 887,386 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 192 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 43.2% C: 38.5% G: 13.5% T: 4.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 189 50.3 0 189 4 1 12.6 0 1 5 1 3.1 0 1 7 1 0.2 0 1