This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.96 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 264,897 (9.1%) Reads that were too short: 57,591 (2.0%) Reads written (passing filters): 207,306 (7.1%) Total basepairs processed: 881,011,047 bp Total written (filtered): 33,508,075 bp (3.8%) === Adapter 1 === Sequence: CACTCT; Type: regular 5'; Length: 6; Trimmed: 264897 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 316 714.6 0 316 7 3250 714.6 0 3250 8 199 714.6 0 199 9 13194 714.6 0 13194 10 76 714.6 0 76 11 17 714.6 0 17 12 47 714.6 0 47 13 24 714.6 0 24 14 26 714.6 0 26 15 22 714.6 0 22 16 15 714.6 0 15 17 25 714.6 0 25 18 20 714.6 0 20 19 16 714.6 0 16 20 26 714.6 0 26 21 27 714.6 0 27 22 39 714.6 0 39 23 19 714.6 0 19 24 16 714.6 0 16 25 20 714.6 0 20 26 21 714.6 0 21 27 18 714.6 0 18 28 29 714.6 0 29 29 21 714.6 0 21 30 22 714.6 0 22 31 27 714.6 0 27 32 25 714.6 0 25 33 25 714.6 0 25 34 22 714.6 0 22 35 38 714.6 0 38 36 25 714.6 0 25 37 45 714.6 0 45 38 21 714.6 0 21 39 36 714.6 0 36 40 27 714.6 0 27 41 23 714.6 0 23 42 19 714.6 0 19 43 74 714.6 0 74 44 31 714.6 0 31 45 25 714.6 0 25 46 428 714.6 0 428 47 141 714.6 0 141 48 94 714.6 0 94 49 71 714.6 0 71 50 40 714.6 0 40 51 23 714.6 0 23 52 24 714.6 0 24 53 25 714.6 0 25 54 44 714.6 0 44 55 59 714.6 0 59 56 55 714.6 0 55 57 29 714.6 0 29 58 28 714.6 0 28 59 24 714.6 0 24 60 17 714.6 0 17 61 27 714.6 0 27 62 27 714.6 0 27 63 19 714.6 0 19 64 24 714.6 0 24 65 19 714.6 0 19 66 22 714.6 0 22 67 56 714.6 0 56 68 24 714.6 0 24 69 32 714.6 0 32 70 29 714.6 0 29 71 33 714.6 0 33 72 41 714.6 0 41 73 101 714.6 0 101 74 97 714.6 0 97 75 83 714.6 0 83 76 84 714.6 0 84 77 95 714.6 0 95 78 54 714.6 0 54 79 120 714.6 0 120 80 89 714.6 0 89 81 112 714.6 0 112 82 166 714.6 0 166 83 105 714.6 0 105 84 138 714.6 0 138 85 111 714.6 0 111 86 190 714.6 0 190 87 226 714.6 0 226 88 351 714.6 0 351 89 437 714.6 0 437 90 377 714.6 0 377 91 295 714.6 0 295 92 500 714.6 0 500 93 226 714.6 0 226 94 671 714.6 0 671 95 748 714.6 0 748 96 467 714.6 0 467 97 203 714.6 0 203 98 126 714.6 0 126 99 140 714.6 0 140 100 89 714.6 0 89 101 67 714.6 0 67 102 163 714.6 0 163 103 114 714.6 0 114 104 578 714.6 0 578 105 700 714.6 0 700 106 1150 714.6 0 1150 107 419 714.6 0 419 108 661 714.6 0 661 109 252 714.6 0 252 110 573 714.6 0 573 111 394 714.6 0 394 112 1196 714.6 0 1196 113 930 714.6 0 930 114 459 714.6 0 459 115 186 714.6 0 186 116 127 714.6 0 127 117 101 714.6 0 101 118 154 714.6 0 154 119 238 714.6 0 238 120 278 714.6 0 278 121 293 714.6 0 293 122 278 714.6 0 278 123 217 714.6 0 217 124 315 714.6 0 315 125 353 714.6 0 353 126 516 714.6 0 516 127 1356 714.6 0 1356 128 1491 714.6 0 1491 129 864 714.6 0 864 130 382 714.6 0 382 131 346 714.6 0 346 132 208 714.6 0 208 133 363 714.6 0 363 134 760 714.6 0 760 135 1297 714.6 0 1297 136 528 714.6 0 528 137 272 714.6 0 272 138 507 714.6 0 507 139 592 714.6 0 592 140 650 714.6 0 650 141 564 714.6 0 564 142 638 714.6 0 638 143 653 714.6 0 653 144 1069 714.6 0 1069 145 1970 714.6 0 1970 146 21885 714.6 0 21885 147 16883 714.6 0 16883 148 6014 714.6 0 6014 149 669 714.6 0 669 150 517 714.6 0 517 151 1353 714.6 0 1353 152 2325 714.6 0 2325 153 1330 714.6 0 1330 154 1504 714.6 0 1504 155 948 714.6 0 948 156 1089 714.6 0 1089 157 910 714.6 0 910 158 1113 714.6 0 1113 159 2100 714.6 0 2100 160 2766 714.6 0 2766 161 33055 714.6 0 33055 162 31675 714.6 0 31675 163 9599 714.6 0 9599 164 770 714.6 0 770 165 818 714.6 0 818 166 700 714.6 0 700 167 688 714.6 0 688 168 379 714.6 0 379 169 319 714.6 0 319 170 242 714.6 0 242 171 295 714.6 0 295 172 519 714.6 0 519 173 530 714.6 0 530 174 808 714.6 0 808 175 892 714.6 0 892 176 653 714.6 0 653 177 547 714.6 0 547 178 355 714.6 0 355 179 522 714.6 0 522 180 536 714.6 0 536 181 1152 714.6 0 1152 182 986 714.6 0 986 183 1126 714.6 0 1126 184 584 714.6 0 584 185 435 714.6 0 435 186 347 714.6 0 347 187 447 714.6 0 447 188 380 714.6 0 380 189 398 714.6 0 398 190 484 714.6 0 484 191 400 714.6 0 400 192 760 714.6 0 760 193 410 714.6 0 410 194 422 714.6 0 422 195 238 714.6 0 238 196 372 714.6 0 372 197 414 714.6 0 414 198 349 714.6 0 349 199 350 714.6 0 350 200 406 714.6 0 406 201 492 714.6 0 492 202 821 714.6 0 821 203 463 714.6 0 463 204 391 714.6 0 391 205 455 714.6 0 455 206 490 714.6 0 490 207 467 714.6 0 467 208 459 714.6 0 459 209 416 714.6 0 416 210 346 714.6 0 346 211 309 714.6 0 309 212 378 714.6 0 378 213 476 714.6 0 476 214 560 714.6 0 560 215 628 714.6 0 628 216 585 714.6 0 585 217 578 714.6 0 578 218 637 714.6 0 637 219 613 714.6 0 613 220 502 714.6 0 502 221 637 714.6 0 637 222 663 714.6 0 663 223 750 714.6 0 750 224 455 714.6 0 455 225 504 714.6 0 504 226 661 714.6 0 661 227 477 714.6 0 477 228 616 714.6 0 616 229 488 714.6 0 488 230 581 714.6 0 581 231 626 714.6 0 626 232 731 714.6 0 731 233 634 714.6 0 634 234 542 714.6 0 542 235 396 714.6 0 396 236 354 714.6 0 354 237 341 714.6 0 341 238 549 714.6 0 549 239 838 714.6 0 838 240 1508 714.6 0 1508 241 2311 714.6 0 2311 242 2243 714.6 0 2243 243 1395 714.6 0 1395 244 860 714.6 0 860 245 505 714.6 0 505 246 349 714.6 0 349 247 292 714.6 0 292 248 194 714.6 0 194 249 309 714.6 0 309 250 384 714.6 0 384 251 767 714.6 0 767 252 990 714.6 0 990 253 818 714.6 0 818 254 982 714.6 0 982 255 847 714.6 0 847 256 660 714.6 0 660 257 383 714.6 0 383 258 264 714.6 0 264 259 198 714.6 0 198 260 205 714.6 0 205 261 281 714.6 0 281 262 412 714.6 0 412 263 439 714.6 0 439 264 406 714.6 0 406 265 545 714.6 0 545 266 441 714.6 0 441 267 987 714.6 0 987 268 871 714.6 0 871 269 856 714.6 0 856 270 873 714.6 0 873 271 825 714.6 0 825 272 563 714.6 0 563 273 359 714.6 0 359 274 440 714.6 0 440 275 376 714.6 0 376 276 475 714.6 0 475 277 1354 714.6 0 1354 278 1593 714.6 0 1593 279 1039 714.6 0 1039 280 436 714.6 0 436 281 557 714.6 0 557 282 572 714.6 0 572 283 755 714.6 0 755 284 483 714.6 0 483 285 345 714.6 0 345 286 323 714.6 0 323 287 183 714.6 0 183 288 308 714.6 0 308 289 362 714.6 0 362 290 487 714.6 0 487 291 515 714.6 0 515 292 478 714.6 0 478 293 257 714.6 0 257 294 283 714.6 0 283 295 218 714.6 0 218 296 188 714.6 0 188 297 196 714.6 0 196 298 136 714.6 0 136 299 178 714.6 0 178 300 148 714.6 0 148 301 167 714.6 0 167 Finished in 6.76 s (33 us/read; 1.84 M reads/minute). === Summary === Total reads processed: 207,306 Reads with adapters: 13,724 (6.6%) Reads that were too short: 140 (0.1%) Reads written (passing filters): 13,584 (6.6%) Total basepairs processed: 33,508,075 bp Total written (filtered): 3,729,722 bp (11.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 13724 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 4 0.0 3 0 0 4 16 135 0.0 3 0 124 8 3 17 13342 0.0 3 12933 356 40 13 18 34 0.0 3 2 27 4 1 19 14 0.0 3 1 0 6 7 20 13 0.0 3 1 0 0 12 23 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 44 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 51 9 0.0 3 0 0 0 9 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 55 5 0.0 3 0 0 0 5 56 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 3 0.0 3 0 0 0 3 67 15 0.0 3 0 0 0 15 68 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 82 2 0.0 3 0 0 0 2 85 2 0.0 3 0 0 0 2 87 6 0.0 3 0 0 0 6 90 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 97 2 0.0 3 0 0 0 2 103 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 125 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 127 14 0.0 3 0 0 0 14 128 22 0.0 3 0 0 2 20 129 3 0.0 3 0 0 0 3 130 1 0.0 3 0 0 0 1 131 19 0.0 3 0 0 0 19 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 134 5 0.0 3 0 0 0 5 136 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 140 2 0.0 3 0 0 0 2 141 5 0.0 3 0 0 0 5 142 9 0.0 3 0 0 0 9 143 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.48 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 13,584 Reads with adapters: 830 (6.1%) Reads written (passing filters): 13,584 (100.0%) Total basepairs processed: 3,729,722 bp Total written (filtered): 3,727,226 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 830 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 14.7% C: 6.5% G: 75.8% T: 3.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 824 212.2 0 824 4 6 53.1 0 6