This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.17 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 69,236 (2.4%) Reads that were too short: 20,113 (0.7%) Reads written (passing filters): 49,123 (1.7%) Total basepairs processed: 881,011,047 bp Total written (filtered): 12,237,738 bp (1.4%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 69236 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 840 714.6 0 840 7 14822 714.6 0 14822 8 14228 714.6 0 14228 9 62 714.6 0 62 10 39 714.6 0 39 11 49 714.6 0 49 12 28 714.6 0 28 13 40 714.6 0 40 14 45 714.6 0 45 15 39 714.6 0 39 16 37 714.6 0 37 17 49 714.6 0 49 18 46 714.6 0 46 19 43 714.6 0 43 20 56 714.6 0 56 21 35 714.6 0 35 22 43 714.6 0 43 23 35 714.6 0 35 24 36 714.6 0 36 25 32 714.6 0 32 26 41 714.6 0 41 27 46 714.6 0 46 28 42 714.6 0 42 29 39 714.6 0 39 30 74 714.6 0 74 31 604 714.6 0 604 32 1706 714.6 0 1706 33 63 714.6 0 63 34 32 714.6 0 32 35 34 714.6 0 34 36 33 714.6 0 33 37 48 714.6 0 48 38 54 714.6 0 54 39 50 714.6 0 50 40 40 714.6 0 40 41 40 714.6 0 40 42 29 714.6 0 29 43 32 714.6 0 32 44 34 714.6 0 34 45 34 714.6 0 34 46 38 714.6 0 38 47 32 714.6 0 32 48 44 714.6 0 44 49 25 714.6 0 25 50 41 714.6 0 41 51 35 714.6 0 35 52 31 714.6 0 31 53 45 714.6 0 45 54 58 714.6 0 58 55 43 714.6 0 43 56 23 714.6 0 23 57 26 714.6 0 26 58 34 714.6 0 34 59 37 714.6 0 37 60 27 714.6 0 27 61 30 714.6 0 30 62 46 714.6 0 46 63 56 714.6 0 56 64 402 714.6 0 402 65 142 714.6 0 142 66 187 714.6 0 187 67 25 714.6 0 25 68 39 714.6 0 39 69 21 714.6 0 21 70 28 714.6 0 28 71 29 714.6 0 29 72 10 714.6 0 10 73 27 714.6 0 27 74 28 714.6 0 28 75 21 714.6 0 21 76 22 714.6 0 22 77 21 714.6 0 21 78 21 714.6 0 21 79 19 714.6 0 19 80 26 714.6 0 26 81 34 714.6 0 34 82 24 714.6 0 24 83 78 714.6 0 78 84 85 714.6 0 85 85 84 714.6 0 84 86 46 714.6 0 46 87 35 714.6 0 35 88 34 714.6 0 34 89 63 714.6 0 63 90 54 714.6 0 54 91 42 714.6 0 42 92 97 714.6 0 97 93 107 714.6 0 107 94 62 714.6 0 62 95 64 714.6 0 64 96 92 714.6 0 92 97 31 714.6 0 31 98 42 714.6 0 42 99 63 714.6 0 63 100 61 714.6 0 61 101 118 714.6 0 118 102 75 714.6 0 75 103 67 714.6 0 67 104 40 714.6 0 40 105 36 714.6 0 36 106 29 714.6 0 29 107 17 714.6 0 17 108 30 714.6 0 30 109 31 714.6 0 31 110 52 714.6 0 52 111 44 714.6 0 44 112 48 714.6 0 48 113 41 714.6 0 41 114 49 714.6 0 49 115 36 714.6 0 36 116 71 714.6 0 71 117 112 714.6 0 112 118 73 714.6 0 73 119 31 714.6 0 31 120 48 714.6 0 48 121 64 714.6 0 64 122 67 714.6 0 67 123 94 714.6 0 94 124 68 714.6 0 68 125 62 714.6 0 62 126 69 714.6 0 69 127 51 714.6 0 51 128 74 714.6 0 74 129 80 714.6 0 80 130 93 714.6 0 93 131 136 714.6 0 136 132 125 714.6 0 125 133 98 714.6 0 98 134 68 714.6 0 68 135 110 714.6 0 110 136 150 714.6 0 150 137 248 714.6 0 248 138 127 714.6 0 127 139 105 714.6 0 105 140 92 714.6 0 92 141 84 714.6 0 84 142 82 714.6 0 82 143 97 714.6 0 97 144 107 714.6 0 107 145 106 714.6 0 106 146 99 714.6 0 99 147 243 714.6 0 243 148 183 714.6 0 183 149 161 714.6 0 161 150 126 714.6 0 126 151 112 714.6 0 112 152 135 714.6 0 135 153 106 714.6 0 106 154 81 714.6 0 81 155 146 714.6 0 146 156 201 714.6 0 201 157 206 714.6 0 206 158 296 714.6 0 296 159 198 714.6 0 198 160 176 714.6 0 176 161 176 714.6 0 176 162 149 714.6 0 149 163 131 714.6 0 131 164 94 714.6 0 94 165 65 714.6 0 65 166 127 714.6 0 127 167 249 714.6 0 249 168 234 714.6 0 234 169 142 714.6 0 142 170 167 714.6 0 167 171 163 714.6 0 163 172 129 714.6 0 129 173 158 714.6 0 158 174 89 714.6 0 89 175 289 714.6 0 289 176 586 714.6 0 586 177 358 714.6 0 358 178 192 714.6 0 192 179 123 714.6 0 123 180 87 714.6 0 87 181 147 714.6 0 147 182 136 714.6 0 136 183 177 714.6 0 177 184 191 714.6 0 191 185 136 714.6 0 136 186 198 714.6 0 198 187 162 714.6 0 162 188 354 714.6 0 354 189 159 714.6 0 159 190 109 714.6 0 109 191 70 714.6 0 70 192 94 714.6 0 94 193 101 714.6 0 101 194 108 714.6 0 108 195 107 714.6 0 107 196 86 714.6 0 86 197 84 714.6 0 84 198 74 714.6 0 74 199 119 714.6 0 119 200 137 714.6 0 137 201 76 714.6 0 76 202 60 714.6 0 60 203 69 714.6 0 69 204 116 714.6 0 116 205 134 714.6 0 134 206 124 714.6 0 124 207 102 714.6 0 102 208 104 714.6 0 104 209 114 714.6 0 114 210 139 714.6 0 139 211 123 714.6 0 123 212 103 714.6 0 103 213 153 714.6 0 153 214 163 714.6 0 163 215 181 714.6 0 181 216 299 714.6 0 299 217 191 714.6 0 191 218 170 714.6 0 170 219 191 714.6 0 191 220 185 714.6 0 185 221 248 714.6 0 248 222 265 714.6 0 265 223 417 714.6 0 417 224 260 714.6 0 260 225 310 714.6 0 310 226 181 714.6 0 181 227 234 714.6 0 234 228 217 714.6 0 217 229 206 714.6 0 206 230 153 714.6 0 153 231 115 714.6 0 115 232 170 714.6 0 170 233 191 714.6 0 191 234 142 714.6 0 142 235 161 714.6 0 161 236 212 714.6 0 212 237 218 714.6 0 218 238 231 714.6 0 231 239 226 714.6 0 226 240 175 714.6 0 175 241 246 714.6 0 246 242 192 714.6 0 192 243 324 714.6 0 324 244 282 714.6 0 282 245 322 714.6 0 322 246 157 714.6 0 157 247 355 714.6 0 355 248 288 714.6 0 288 249 213 714.6 0 213 250 81 714.6 0 81 251 53 714.6 0 53 252 125 714.6 0 125 253 293 714.6 0 293 254 349 714.6 0 349 255 359 714.6 0 359 256 285 714.6 0 285 257 201 714.6 0 201 258 192 714.6 0 192 259 190 714.6 0 190 260 127 714.6 0 127 261 108 714.6 0 108 262 205 714.6 0 205 263 279 714.6 0 279 264 283 714.6 0 283 265 330 714.6 0 330 266 199 714.6 0 199 267 223 714.6 0 223 268 416 714.6 0 416 269 210 714.6 0 210 270 175 714.6 0 175 271 87 714.6 0 87 272 139 714.6 0 139 273 283 714.6 0 283 274 269 714.6 0 269 275 270 714.6 0 270 276 170 714.6 0 170 277 161 714.6 0 161 278 148 714.6 0 148 279 160 714.6 0 160 280 292 714.6 0 292 281 215 714.6 0 215 282 180 714.6 0 180 283 208 714.6 0 208 284 214 714.6 0 214 285 264 714.6 0 264 286 248 714.6 0 248 287 373 714.6 0 373 288 316 714.6 0 316 289 146 714.6 0 146 290 84 714.6 0 84 291 125 714.6 0 125 292 234 714.6 0 234 293 359 714.6 0 359 294 237 714.6 0 237 295 115 714.6 0 115 296 82 714.6 0 82 297 54 714.6 0 54 298 94 714.6 0 94 299 132 714.6 0 132 300 226 714.6 0 226 301 113 714.6 0 113 Finished in 2.40 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 49,123 Reads with adapters: 14,838 (30.2%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 14,829 (30.2%) Total basepairs processed: 12,237,738 bp Total written (filtered): 4,048,026 bp (33.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 14838 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 10 0.0 3 0 0 9 1 19 148 0.0 3 0 148 20 14526 0.0 4 14350 168 6 1 1 21 45 0.0 4 5 37 3 22 2 0.0 4 0 1 0 0 1 23 84 0.0 4 79 0 0 3 2 24 2 0.0 4 0 1 0 0 1 31 3 0.0 4 0 0 0 0 3 43 1 0.0 4 0 0 0 0 1 44 1 0.0 4 1 55 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 62 2 0.0 4 1 0 0 0 1 64 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 1 114 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 139 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.52 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 14,829 Reads with adapters: 2,521 (17.0%) Reads written (passing filters): 14,829 (100.0%) Total basepairs processed: 4,048,026 bp Total written (filtered): 4,032,163 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2521 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.6% C: 0.4% G: 98.4% T: 0.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 8 231.7 0 8 4 1682 57.9 0 1682 5 75 14.5 0 75 6 8 3.6 0 8 7 8 0.9 0 8 9 3 0.1 0 0 3 10 17 0.0 1 1 16 11 715 0.0 1 4 711 12 1 0.0 1 0 1 14 2 0.0 1 0 2 259 1 0.0 1 1 271 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.