This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.09 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 49,338 (1.7%) Reads that were too short: 4,373 (0.1%) Reads written (passing filters): 44,965 (1.5%) Total basepairs processed: 881,011,047 bp Total written (filtered): 11,837,758 bp (1.3%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 49338 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2068 714.6 0 2068 7 1570 714.6 0 1570 8 14729 714.6 0 14729 9 10238 714.6 0 10238 10 75 714.6 0 75 11 41 714.6 0 41 12 41 714.6 0 41 13 41 714.6 0 41 14 47 714.6 0 47 15 47 714.6 0 47 16 51 714.6 0 51 17 42 714.6 0 42 18 36 714.6 0 36 19 50 714.6 0 50 20 32 714.6 0 32 21 38 714.6 0 38 22 50 714.6 0 50 23 38 714.6 0 38 24 27 714.6 0 27 25 38 714.6 0 38 26 38 714.6 0 38 27 37 714.6 0 37 28 44 714.6 0 44 29 46 714.6 0 46 30 38 714.6 0 38 31 35 714.6 0 35 32 40 714.6 0 40 33 42 714.6 0 42 34 110 714.6 0 110 35 61 714.6 0 61 36 36 714.6 0 36 37 51 714.6 0 51 38 63 714.6 0 63 39 32 714.6 0 32 40 44 714.6 0 44 41 39 714.6 0 39 42 42 714.6 0 42 43 46 714.6 0 46 44 51 714.6 0 51 45 23 714.6 0 23 46 65 714.6 0 65 47 5098 714.6 0 5098 48 808 714.6 0 808 49 134 714.6 0 134 50 257 714.6 0 257 51 34 714.6 0 34 52 26 714.6 0 26 53 26 714.6 0 26 54 34 714.6 0 34 55 44 714.6 0 44 56 24 714.6 0 24 57 20 714.6 0 20 58 36 714.6 0 36 59 30 714.6 0 30 60 30 714.6 0 30 61 27 714.6 0 27 62 23 714.6 0 23 63 23 714.6 0 23 64 27 714.6 0 27 65 36 714.6 0 36 66 23 714.6 0 23 67 26 714.6 0 26 68 37 714.6 0 37 69 19 714.6 0 19 70 22 714.6 0 22 71 27 714.6 0 27 72 21 714.6 0 21 73 20 714.6 0 20 74 19 714.6 0 19 75 18 714.6 0 18 76 28 714.6 0 28 77 31 714.6 0 31 78 28 714.6 0 28 79 24 714.6 0 24 80 34 714.6 0 34 81 47 714.6 0 47 82 59 714.6 0 59 83 64 714.6 0 64 84 72 714.6 0 72 85 75 714.6 0 75 86 40 714.6 0 40 87 80 714.6 0 80 88 56 714.6 0 56 89 49 714.6 0 49 90 54 714.6 0 54 91 41 714.6 0 41 92 34 714.6 0 34 93 37 714.6 0 37 94 42 714.6 0 42 95 58 714.6 0 58 96 165 714.6 0 165 97 75 714.6 0 75 98 56 714.6 0 56 99 34 714.6 0 34 100 40 714.6 0 40 101 34 714.6 0 34 102 58 714.6 0 58 103 97 714.6 0 97 104 69 714.6 0 69 105 66 714.6 0 66 106 20 714.6 0 20 107 14 714.6 0 14 108 16 714.6 0 16 109 18 714.6 0 18 110 22 714.6 0 22 111 32 714.6 0 32 112 29 714.6 0 29 113 38 714.6 0 38 114 64 714.6 0 64 115 34 714.6 0 34 116 24 714.6 0 24 117 36 714.6 0 36 118 56 714.6 0 56 119 49 714.6 0 49 120 40 714.6 0 40 121 33 714.6 0 33 122 26 714.6 0 26 123 28 714.6 0 28 124 32 714.6 0 32 125 24 714.6 0 24 126 30 714.6 0 30 127 49 714.6 0 49 128 51 714.6 0 51 129 37 714.6 0 37 130 21 714.6 0 21 131 31 714.6 0 31 132 49 714.6 0 49 133 59 714.6 0 59 134 34 714.6 0 34 135 31 714.6 0 31 136 47 714.6 0 47 137 40 714.6 0 40 138 40 714.6 0 40 139 37 714.6 0 37 140 60 714.6 0 60 141 43 714.6 0 43 142 44 714.6 0 44 143 51 714.6 0 51 144 63 714.6 0 63 145 77 714.6 0 77 146 562 714.6 0 562 147 1937 714.6 0 1937 148 113 714.6 0 113 149 41 714.6 0 41 150 39 714.6 0 39 151 25 714.6 0 25 152 17 714.6 0 17 153 23 714.6 0 23 154 25 714.6 0 25 155 20 714.6 0 20 156 24 714.6 0 24 157 22 714.6 0 22 158 36 714.6 0 36 159 33 714.6 0 33 160 32 714.6 0 32 161 24 714.6 0 24 162 22 714.6 0 22 163 24 714.6 0 24 164 21 714.6 0 21 165 26 714.6 0 26 166 19 714.6 0 19 167 22 714.6 0 22 168 43 714.6 0 43 169 23 714.6 0 23 170 39 714.6 0 39 171 20 714.6 0 20 172 23 714.6 0 23 173 30 714.6 0 30 174 15 714.6 0 15 175 23 714.6 0 23 176 32 714.6 0 32 177 40 714.6 0 40 178 42 714.6 0 42 179 35 714.6 0 35 180 33 714.6 0 33 181 51 714.6 0 51 182 35 714.6 0 35 183 34 714.6 0 34 184 29 714.6 0 29 185 33 714.6 0 33 186 37 714.6 0 37 187 32 714.6 0 32 188 25 714.6 0 25 189 27 714.6 0 27 190 30 714.6 0 30 191 31 714.6 0 31 192 41 714.6 0 41 193 68 714.6 0 68 194 40 714.6 0 40 195 137 714.6 0 137 196 156 714.6 0 156 197 98 714.6 0 98 198 53 714.6 0 53 199 30 714.6 0 30 200 30 714.6 0 30 201 16 714.6 0 16 202 18 714.6 0 18 203 15 714.6 0 15 204 21 714.6 0 21 205 24 714.6 0 24 206 41 714.6 0 41 207 34 714.6 0 34 208 32 714.6 0 32 209 20 714.6 0 20 210 27 714.6 0 27 211 29 714.6 0 29 212 29 714.6 0 29 213 59 714.6 0 59 214 100 714.6 0 100 215 55 714.6 0 55 216 49 714.6 0 49 217 39 714.6 0 39 218 54 714.6 0 54 219 60 714.6 0 60 220 24 714.6 0 24 221 31 714.6 0 31 222 38 714.6 0 38 223 24 714.6 0 24 224 32 714.6 0 32 225 48 714.6 0 48 226 37 714.6 0 37 227 46 714.6 0 46 228 28 714.6 0 28 229 42 714.6 0 42 230 31 714.6 0 31 231 48 714.6 0 48 232 19 714.6 0 19 233 22 714.6 0 22 234 30 714.6 0 30 235 52 714.6 0 52 236 72 714.6 0 72 237 46 714.6 0 46 238 61 714.6 0 61 239 64 714.6 0 64 240 42 714.6 0 42 241 25 714.6 0 25 242 20 714.6 0 20 243 49 714.6 0 49 244 80 714.6 0 80 245 117 714.6 0 117 246 95 714.6 0 95 247 174 714.6 0 174 248 45 714.6 0 45 249 10 714.6 0 10 250 20 714.6 0 20 251 8 714.6 0 8 252 26 714.6 0 26 253 30 714.6 0 30 254 49 714.6 0 49 255 49 714.6 0 49 256 75 714.6 0 75 257 67 714.6 0 67 258 39 714.6 0 39 259 44 714.6 0 44 260 91 714.6 0 91 261 19 714.6 0 19 262 25 714.6 0 25 263 31 714.6 0 31 264 26 714.6 0 26 265 34 714.6 0 34 266 42 714.6 0 42 267 46 714.6 0 46 268 41 714.6 0 41 269 22 714.6 0 22 270 23 714.6 0 23 271 18 714.6 0 18 272 19 714.6 0 19 273 24 714.6 0 24 274 27 714.6 0 27 275 27 714.6 0 27 276 14 714.6 0 14 277 16 714.6 0 16 278 23 714.6 0 23 279 82 714.6 0 82 280 55 714.6 0 55 281 57 714.6 0 57 282 42 714.6 0 42 283 27 714.6 0 27 284 55 714.6 0 55 285 97 714.6 0 97 286 75 714.6 0 75 287 100 714.6 0 100 288 70 714.6 0 70 289 33 714.6 0 33 290 28 714.6 0 28 291 39 714.6 0 39 292 46 714.6 0 46 293 77 714.6 0 77 294 37 714.6 0 37 295 33 714.6 0 33 296 36 714.6 0 36 297 35 714.6 0 35 298 136 714.6 0 136 299 23 714.6 0 23 300 27 714.6 0 27 301 30 714.6 0 30 Finished in 2.07 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 44,965 Reads with adapters: 16,366 (36.4%) Reads that were too short: 26 (0.1%) Reads written (passing filters): 16,340 (36.3%) Total basepairs processed: 11,837,758 bp Total written (filtered): 4,501,409 bp (38.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 16366 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 9 0.0 3 0 0 7 2 16 111 0.0 3 0 94 10 7 17 14294 0.0 3 13701 512 61 20 18 89 0.0 3 3 57 23 6 19 1747 0.0 3 1642 56 21 28 20 24 0.0 3 1 2 1 20 21 1 0.0 3 0 0 1 22 3 0.0 3 0 0 0 3 25 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 29 14 0.0 3 0 0 0 14 30 1 0.0 3 0 0 0 1 33 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 45 17 0.0 3 0 0 0 17 48 3 0.0 3 0 0 0 3 54 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 123 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 217 12 0.0 3 0 0 1 11 233 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.58 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 16,340 Reads with adapters: 217 (1.3%) Reads written (passing filters): 16,340 (100.0%) Total basepairs processed: 4,501,409 bp Total written (filtered): 4,500,722 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 217 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 41.9% C: 10.1% G: 33.2% T: 14.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 184 255.3 0 184 4 30 63.8 0 30 5 3 16.0 0 3