This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.18 s (25 us/read; 2.37 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 43,400 (1.5%) Reads that were too short: 10,760 (0.4%) Reads written (passing filters): 32,640 (1.1%) Total basepairs processed: 881,011,047 bp Total written (filtered): 8,226,564 bp (0.9%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 43400 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2358 714.6 0 2358 7 1924 714.6 0 1924 8 14655 714.6 0 14655 9 93 714.6 0 93 10 81 714.6 0 81 11 36 714.6 0 36 12 53 714.6 0 53 13 37 714.6 0 37 14 58 714.6 0 58 15 46 714.6 0 46 16 52 714.6 0 52 17 209 714.6 0 209 18 59 714.6 0 59 19 55 714.6 0 55 20 56 714.6 0 56 21 32 714.6 0 32 22 28 714.6 0 28 23 46 714.6 0 46 24 42 714.6 0 42 25 44 714.6 0 44 26 46 714.6 0 46 27 58 714.6 0 58 28 44 714.6 0 44 29 58 714.6 0 58 30 83 714.6 0 83 31 40 714.6 0 40 32 34 714.6 0 34 33 80 714.6 0 80 34 35 714.6 0 35 35 37 714.6 0 37 36 55 714.6 0 55 37 38 714.6 0 38 38 45 714.6 0 45 39 50 714.6 0 50 40 48 714.6 0 48 41 35 714.6 0 35 42 99 714.6 0 99 43 54 714.6 0 54 44 17 714.6 0 17 45 36 714.6 0 36 46 50 714.6 0 50 47 55 714.6 0 55 48 55 714.6 0 55 49 57 714.6 0 57 50 33 714.6 0 33 51 55 714.6 0 55 52 42 714.6 0 42 53 88 714.6 0 88 54 46 714.6 0 46 55 38 714.6 0 38 56 49 714.6 0 49 57 63 714.6 0 63 58 46 714.6 0 46 59 41 714.6 0 41 60 70 714.6 0 70 61 68 714.6 0 68 62 56 714.6 0 56 63 59 714.6 0 59 64 37 714.6 0 37 65 46 714.6 0 46 66 40 714.6 0 40 67 58 714.6 0 58 68 45 714.6 0 45 69 34 714.6 0 34 70 38 714.6 0 38 71 49 714.6 0 49 72 46 714.6 0 46 73 286 714.6 0 286 74 326 714.6 0 326 75 620 714.6 0 620 76 40 714.6 0 40 77 235 714.6 0 235 78 329 714.6 0 329 79 301 714.6 0 301 80 30 714.6 0 30 81 32 714.6 0 32 82 41 714.6 0 41 83 31 714.6 0 31 84 45 714.6 0 45 85 43 714.6 0 43 86 63 714.6 0 63 87 37 714.6 0 37 88 39 714.6 0 39 89 38 714.6 0 38 90 49 714.6 0 49 91 35 714.6 0 35 92 59 714.6 0 59 93 64 714.6 0 64 94 58 714.6 0 58 95 39 714.6 0 39 96 43 714.6 0 43 97 46 714.6 0 46 98 43 714.6 0 43 99 48 714.6 0 48 100 38 714.6 0 38 101 26 714.6 0 26 102 24 714.6 0 24 103 35 714.6 0 35 104 32 714.6 0 32 105 32 714.6 0 32 106 65 714.6 0 65 107 224 714.6 0 224 108 33 714.6 0 33 109 33 714.6 0 33 110 44 714.6 0 44 111 28 714.6 0 28 112 41 714.6 0 41 113 45 714.6 0 45 114 55 714.6 0 55 115 65 714.6 0 65 116 47 714.6 0 47 117 47 714.6 0 47 118 44 714.6 0 44 119 33 714.6 0 33 120 65 714.6 0 65 121 55 714.6 0 55 122 39 714.6 0 39 123 27 714.6 0 27 124 48 714.6 0 48 125 57 714.6 0 57 126 39 714.6 0 39 127 106 714.6 0 106 128 405 714.6 0 405 129 249 714.6 0 249 130 117 714.6 0 117 131 59 714.6 0 59 132 53 714.6 0 53 133 78 714.6 0 78 134 190 714.6 0 190 135 163 714.6 0 163 136 257 714.6 0 257 137 131 714.6 0 131 138 54 714.6 0 54 139 65 714.6 0 65 140 66 714.6 0 66 141 31 714.6 0 31 142 41 714.6 0 41 143 48 714.6 0 48 144 76 714.6 0 76 145 56 714.6 0 56 146 281 714.6 0 281 147 134 714.6 0 134 148 408 714.6 0 408 149 39 714.6 0 39 150 78 714.6 0 78 151 36 714.6 0 36 152 42 714.6 0 42 153 38 714.6 0 38 154 33 714.6 0 33 155 95 714.6 0 95 156 93 714.6 0 93 157 60 714.6 0 60 158 41 714.6 0 41 159 81 714.6 0 81 160 64 714.6 0 64 161 52 714.6 0 52 162 30 714.6 0 30 163 41 714.6 0 41 164 55 714.6 0 55 165 69 714.6 0 69 166 81 714.6 0 81 167 50 714.6 0 50 168 59 714.6 0 59 169 44 714.6 0 44 170 54 714.6 0 54 171 39 714.6 0 39 172 29 714.6 0 29 173 36 714.6 0 36 174 42 714.6 0 42 175 34 714.6 0 34 176 37 714.6 0 37 177 41 714.6 0 41 178 41 714.6 0 41 179 51 714.6 0 51 180 57 714.6 0 57 181 60 714.6 0 60 182 53 714.6 0 53 183 46 714.6 0 46 184 112 714.6 0 112 185 125 714.6 0 125 186 136 714.6 0 136 187 45 714.6 0 45 188 39 714.6 0 39 189 24 714.6 0 24 190 36 714.6 0 36 191 41 714.6 0 41 192 64 714.6 0 64 193 45 714.6 0 45 194 36 714.6 0 36 195 72 714.6 0 72 196 98 714.6 0 98 197 64 714.6 0 64 198 50 714.6 0 50 199 40 714.6 0 40 200 36 714.6 0 36 201 47 714.6 0 47 202 31 714.6 0 31 203 30 714.6 0 30 204 58 714.6 0 58 205 61 714.6 0 61 206 37 714.6 0 37 207 45 714.6 0 45 208 42 714.6 0 42 209 53 714.6 0 53 210 42 714.6 0 42 211 65 714.6 0 65 212 64 714.6 0 64 213 49 714.6 0 49 214 69 714.6 0 69 215 95 714.6 0 95 216 84 714.6 0 84 217 84 714.6 0 84 218 56 714.6 0 56 219 69 714.6 0 69 220 65 714.6 0 65 221 63 714.6 0 63 222 38 714.6 0 38 223 63 714.6 0 63 224 73 714.6 0 73 225 78 714.6 0 78 226 70 714.6 0 70 227 65 714.6 0 65 228 50 714.6 0 50 229 47 714.6 0 47 230 46 714.6 0 46 231 62 714.6 0 62 232 54 714.6 0 54 233 78 714.6 0 78 234 120 714.6 0 120 235 249 714.6 0 249 236 3100 714.6 0 3100 237 1670 714.6 0 1670 238 687 714.6 0 687 239 46 714.6 0 46 240 44 714.6 0 44 241 41 714.6 0 41 242 28 714.6 0 28 243 29 714.6 0 29 244 47 714.6 0 47 245 50 714.6 0 50 246 74 714.6 0 74 247 65 714.6 0 65 248 74 714.6 0 74 249 34 714.6 0 34 250 45 714.6 0 45 251 23 714.6 0 23 252 48 714.6 0 48 253 68 714.6 0 68 254 135 714.6 0 135 255 104 714.6 0 104 256 75 714.6 0 75 257 36 714.6 0 36 258 35 714.6 0 35 259 27 714.6 0 27 260 25 714.6 0 25 261 28 714.6 0 28 262 27 714.6 0 27 263 39 714.6 0 39 264 43 714.6 0 43 265 41 714.6 0 41 266 54 714.6 0 54 267 46 714.6 0 46 268 25 714.6 0 25 269 28 714.6 0 28 270 37 714.6 0 37 271 26 714.6 0 26 272 76 714.6 0 76 273 44 714.6 0 44 274 36 714.6 0 36 275 24 714.6 0 24 276 36 714.6 0 36 277 33 714.6 0 33 278 41 714.6 0 41 279 41 714.6 0 41 280 70 714.6 0 70 281 68 714.6 0 68 282 141 714.6 0 141 283 78 714.6 0 78 284 56 714.6 0 56 285 87 714.6 0 87 286 58 714.6 0 58 287 32 714.6 0 32 288 13 714.6 0 13 289 26 714.6 0 26 290 15 714.6 0 15 291 36 714.6 0 36 292 26 714.6 0 26 293 33 714.6 0 33 294 38 714.6 0 38 295 38 714.6 0 38 296 78 714.6 0 78 297 122 714.6 0 122 298 62 714.6 0 62 299 27 714.6 0 27 300 28 714.6 0 28 301 38 714.6 0 38 Finished in 1.43 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 32,640 Reads with adapters: 1,704 (5.2%) Reads that were too short: 30 (0.1%) Reads written (passing filters): 1,674 (5.1%) Total basepairs processed: 8,226,564 bp Total written (filtered): 447,608 bp (5.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1704 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 12 0.0 3 0 6 3 3 17 1540 0.0 3 1479 56 2 3 18 8 0.0 3 3 3 2 19 28 0.0 3 0 0 0 28 20 3 0.0 3 0 0 0 3 23 1 0.0 3 0 0 0 1 27 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 39 12 0.0 3 0 0 0 12 43 1 0.0 3 0 0 0 1 45 9 0.0 3 0 0 0 9 47 2 0.0 3 0 0 0 2 48 3 0.0 3 0 0 0 3 49 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 64 5 0.0 3 0 0 1 4 68 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 94 9 0.0 3 0 0 0 9 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 114 6 0.0 3 0 0 0 6 115 4 0.0 3 0 0 0 4 117 5 0.0 3 0 0 1 4 118 3 0.0 3 0 0 0 3 121 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 1 135 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 192 4 0.0 3 0 0 0 4 217 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 3 0.0 3 0 0 0 3 283 3 0.0 3 0 0 0 3 288 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 1,674 Reads with adapters: 154 (9.2%) Reads written (passing filters): 1,674 (100.0%) Total basepairs processed: 447,608 bp Total written (filtered): 447,146 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 154 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 30.5% C: 57.8% G: 7.8% T: 3.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 154 26.2 0 154