This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.06 s (25 us/read; 2.37 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 66,449 (2.3%) Reads that were too short: 21,211 (0.7%) Reads written (passing filters): 45,238 (1.5%) Total basepairs processed: 881,011,047 bp Total written (filtered): 6,352,577 bp (0.7%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 66449 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 175 714.6 0 175 7 29 714.6 0 29 8 115 714.6 0 115 9 1578 714.6 0 1578 10 13 714.6 0 13 11 17 714.6 0 17 12 21 714.6 0 21 13 13 714.6 0 13 14 19 714.6 0 19 15 14 714.6 0 14 16 16 714.6 0 16 17 20 714.6 0 20 18 16 714.6 0 16 19 20 714.6 0 20 20 15 714.6 0 15 21 27 714.6 0 27 22 26 714.6 0 26 23 19 714.6 0 19 24 12 714.6 0 12 25 14 714.6 0 14 26 21 714.6 0 21 27 12 714.6 0 12 28 23 714.6 0 23 29 28 714.6 0 28 30 25 714.6 0 25 31 19 714.6 0 19 32 55 714.6 0 55 33 52 714.6 0 52 34 17 714.6 0 17 35 13 714.6 0 13 36 11 714.6 0 11 37 23 714.6 0 23 38 25 714.6 0 25 39 26 714.6 0 26 40 25 714.6 0 25 41 23 714.6 0 23 42 18 714.6 0 18 43 22 714.6 0 22 44 27 714.6 0 27 45 18 714.6 0 18 46 16 714.6 0 16 47 29 714.6 0 29 48 20 714.6 0 20 49 24 714.6 0 24 50 19 714.6 0 19 51 22 714.6 0 22 52 16 714.6 0 16 53 18 714.6 0 18 54 23 714.6 0 23 55 26 714.6 0 26 56 15 714.6 0 15 57 24 714.6 0 24 58 17 714.6 0 17 59 17 714.6 0 17 60 21 714.6 0 21 61 16 714.6 0 16 62 20 714.6 0 20 63 11 714.6 0 11 64 18 714.6 0 18 65 21 714.6 0 21 66 25 714.6 0 25 67 19 714.6 0 19 68 22 714.6 0 22 69 91 714.6 0 91 70 22 714.6 0 22 71 27 714.6 0 27 72 13 714.6 0 13 73 20 714.6 0 20 74 27 714.6 0 27 75 16 714.6 0 16 76 21 714.6 0 21 77 21 714.6 0 21 78 14 714.6 0 14 79 26 714.6 0 26 80 86 714.6 0 86 81 41 714.6 0 41 82 35 714.6 0 35 83 21 714.6 0 21 84 23 714.6 0 23 85 23 714.6 0 23 86 24 714.6 0 24 87 21 714.6 0 21 88 37 714.6 0 37 89 18 714.6 0 18 90 20 714.6 0 20 91 17 714.6 0 17 92 15 714.6 0 15 93 22 714.6 0 22 94 28 714.6 0 28 95 48 714.6 0 48 96 123 714.6 0 123 97 251 714.6 0 251 98 73 714.6 0 73 99 49 714.6 0 49 100 59 714.6 0 59 101 59 714.6 0 59 102 43 714.6 0 43 103 46 714.6 0 46 104 34 714.6 0 34 105 33 714.6 0 33 106 16 714.6 0 16 107 37 714.6 0 37 108 233 714.6 0 233 109 18 714.6 0 18 110 28 714.6 0 28 111 44 714.6 0 44 112 34 714.6 0 34 113 61 714.6 0 61 114 67 714.6 0 67 115 42 714.6 0 42 116 43 714.6 0 43 117 369 714.6 0 369 118 270 714.6 0 270 119 236 714.6 0 236 120 54 714.6 0 54 121 39 714.6 0 39 122 64 714.6 0 64 123 42 714.6 0 42 124 45 714.6 0 45 125 55 714.6 0 55 126 66 714.6 0 66 127 102 714.6 0 102 128 90 714.6 0 90 129 72 714.6 0 72 130 67 714.6 0 67 131 157 714.6 0 157 132 97 714.6 0 97 133 179 714.6 0 179 134 119 714.6 0 119 135 90 714.6 0 90 136 135 714.6 0 135 137 103 714.6 0 103 138 88 714.6 0 88 139 115 714.6 0 115 140 107 714.6 0 107 141 84 714.6 0 84 142 67 714.6 0 67 143 74 714.6 0 74 144 59 714.6 0 59 145 47 714.6 0 47 146 91 714.6 0 91 147 101 714.6 0 101 148 65 714.6 0 65 149 54 714.6 0 54 150 50 714.6 0 50 151 37 714.6 0 37 152 68 714.6 0 68 153 46 714.6 0 46 154 56 714.6 0 56 155 73 714.6 0 73 156 195 714.6 0 195 157 151 714.6 0 151 158 312 714.6 0 312 159 86 714.6 0 86 160 79 714.6 0 79 161 145 714.6 0 145 162 111 714.6 0 111 163 127 714.6 0 127 164 138 714.6 0 138 165 277 714.6 0 277 166 176 714.6 0 176 167 168 714.6 0 168 168 207 714.6 0 207 169 231 714.6 0 231 170 165 714.6 0 165 171 201 714.6 0 201 172 175 714.6 0 175 173 158 714.6 0 158 174 201 714.6 0 201 175 411 714.6 0 411 176 538 714.6 0 538 177 1060 714.6 0 1060 178 2164 714.6 0 2164 179 18903 714.6 0 18903 180 1416 714.6 0 1416 181 1691 714.6 0 1691 182 1332 714.6 0 1332 183 404 714.6 0 404 184 2014 714.6 0 2014 185 143 714.6 0 143 186 86 714.6 0 86 187 110 714.6 0 110 188 109 714.6 0 109 189 89 714.6 0 89 190 80 714.6 0 80 191 60 714.6 0 60 192 67 714.6 0 67 193 94 714.6 0 94 194 196 714.6 0 196 195 317 714.6 0 317 196 523 714.6 0 523 197 356 714.6 0 356 198 274 714.6 0 274 199 92 714.6 0 92 200 172 714.6 0 172 201 210 714.6 0 210 202 265 714.6 0 265 203 195 714.6 0 195 204 125 714.6 0 125 205 82 714.6 0 82 206 57 714.6 0 57 207 77 714.6 0 77 208 103 714.6 0 103 209 76 714.6 0 76 210 50 714.6 0 50 211 60 714.6 0 60 212 74 714.6 0 74 213 120 714.6 0 120 214 111 714.6 0 111 215 121 714.6 0 121 216 112 714.6 0 112 217 111 714.6 0 111 218 53 714.6 0 53 219 85 714.6 0 85 220 86 714.6 0 86 221 96 714.6 0 96 222 59 714.6 0 59 223 78 714.6 0 78 224 119 714.6 0 119 225 127 714.6 0 127 226 84 714.6 0 84 227 70 714.6 0 70 228 95 714.6 0 95 229 153 714.6 0 153 230 86 714.6 0 86 231 68 714.6 0 68 232 66 714.6 0 66 233 63 714.6 0 63 234 92 714.6 0 92 235 90 714.6 0 90 236 110 714.6 0 110 237 97 714.6 0 97 238 180 714.6 0 180 239 1492 714.6 0 1492 240 311 714.6 0 311 241 526 714.6 0 526 242 75 714.6 0 75 243 91 714.6 0 91 244 70 714.6 0 70 245 93 714.6 0 93 246 92 714.6 0 92 247 73 714.6 0 73 248 125 714.6 0 125 249 148 714.6 0 148 250 109 714.6 0 109 251 61 714.6 0 61 252 67 714.6 0 67 253 93 714.6 0 93 254 103 714.6 0 103 255 78 714.6 0 78 256 103 714.6 0 103 257 147 714.6 0 147 258 204 714.6 0 204 259 505 714.6 0 505 260 794 714.6 0 794 261 979 714.6 0 979 262 156 714.6 0 156 263 420 714.6 0 420 264 971 714.6 0 971 265 1213 714.6 0 1213 266 1264 714.6 0 1264 267 845 714.6 0 845 268 108 714.6 0 108 269 68 714.6 0 68 270 54 714.6 0 54 271 26 714.6 0 26 272 32 714.6 0 32 273 41 714.6 0 41 274 62 714.6 0 62 275 99 714.6 0 99 276 76 714.6 0 76 277 65 714.6 0 65 278 58 714.6 0 58 279 93 714.6 0 93 280 100 714.6 0 100 281 102 714.6 0 102 282 57 714.6 0 57 283 53 714.6 0 53 284 49 714.6 0 49 285 52 714.6 0 52 286 63 714.6 0 63 287 106 714.6 0 106 288 149 714.6 0 149 289 105 714.6 0 105 290 51 714.6 0 51 291 215 714.6 0 215 292 796 714.6 0 796 293 1388 714.6 0 1388 294 849 714.6 0 849 295 836 714.6 0 836 296 199 714.6 0 199 297 98 714.6 0 98 298 46 714.6 0 46 299 73 714.6 0 73 300 93 714.6 0 93 301 45 714.6 0 45 Finished in 1.53 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 45,238 Reads with adapters: 1,617 (3.6%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 1,613 (3.6%) Total basepairs processed: 6,352,577 bp Total written (filtered): 438,124 bp (6.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1617 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 1 0.0 3 0 0 0 0 1 19 18 0.0 3 0 17 1 20 1583 0.0 4 1561 18 1 0 3 21 4 0.0 4 1 1 0 1 1 22 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (37 us/read; 1.61 M reads/minute). === Summary === Total reads processed: 1,613 Reads with adapters: 46 (2.9%) Reads written (passing filters): 1,613 (100.0%) Total basepairs processed: 438,124 bp Total written (filtered): 436,640 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 46 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 10.9% C: 2.2% G: 87.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 1 6.3 0 1 9 4 0.0 0 1 3 10 22 0.0 1 1 21 11 14 0.0 1 0 14 12 1 0.0 1 0 1 259 2 0.0 1 2 270 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.