This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAACAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.59 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 482,685 (16.5%) Reads that were too short: 197,030 (6.7%) Reads written (passing filters): 285,655 (9.8%) Total basepairs processed: 881,011,047 bp Total written (filtered): 55,785,071 bp (6.3%) === Adapter 1 === Sequence: TAACAT; Type: regular 5'; Length: 6; Trimmed: 482685 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 931 714.6 0 931 7 27793 714.6 0 27793 8 383 714.6 0 383 9 20570 714.6 0 20570 10 108 714.6 0 108 11 8 714.6 0 8 12 13 714.6 0 13 13 30 714.6 0 30 14 12 714.6 0 12 15 4 714.6 0 4 16 10 714.6 0 10 17 21 714.6 0 21 18 14 714.6 0 14 19 10 714.6 0 10 20 14 714.6 0 14 21 19 714.6 0 19 22 7 714.6 0 7 23 14 714.6 0 14 24 11 714.6 0 11 25 7 714.6 0 7 26 13 714.6 0 13 27 15 714.6 0 15 28 14 714.6 0 14 29 10 714.6 0 10 30 15 714.6 0 15 31 10 714.6 0 10 32 14 714.6 0 14 33 15 714.6 0 15 34 13 714.6 0 13 35 17 714.6 0 17 36 19 714.6 0 19 37 22 714.6 0 22 38 14 714.6 0 14 39 5 714.6 0 5 40 15 714.6 0 15 41 12 714.6 0 12 42 20 714.6 0 20 43 5 714.6 0 5 44 9 714.6 0 9 45 9 714.6 0 9 46 8 714.6 0 8 47 5 714.6 0 5 48 8 714.6 0 8 49 17 714.6 0 17 50 10 714.6 0 10 51 7 714.6 0 7 52 14 714.6 0 14 53 15 714.6 0 15 54 174 714.6 0 174 55 38347 714.6 0 38347 56 549 714.6 0 549 57 35 714.6 0 35 58 13 714.6 0 13 59 14 714.6 0 14 60 13 714.6 0 13 61 84 714.6 0 84 62 29 714.6 0 29 63 14 714.6 0 14 64 17 714.6 0 17 65 49 714.6 0 49 66 49 714.6 0 49 67 30 714.6 0 30 68 9 714.6 0 9 69 25 714.6 0 25 70 77 714.6 0 77 71 69 714.6 0 69 72 97 714.6 0 97 73 172 714.6 0 172 74 344 714.6 0 344 75 382 714.6 0 382 76 1269 714.6 0 1269 77 793 714.6 0 793 78 478 714.6 0 478 79 541 714.6 0 541 80 926 714.6 0 926 81 1664 714.6 0 1664 82 1618 714.6 0 1618 83 1027 714.6 0 1027 84 1025 714.6 0 1025 85 1131 714.6 0 1131 86 2058 714.6 0 2058 87 1309 714.6 0 1309 88 1191 714.6 0 1191 89 1767 714.6 0 1767 90 858 714.6 0 858 91 777 714.6 0 777 92 843 714.6 0 843 93 1199 714.6 0 1199 94 1013 714.6 0 1013 95 1159 714.6 0 1159 96 682 714.6 0 682 97 1347 714.6 0 1347 98 1076 714.6 0 1076 99 1012 714.6 0 1012 100 950 714.6 0 950 101 1123 714.6 0 1123 102 2381 714.6 0 2381 103 1742 714.6 0 1742 104 1327 714.6 0 1327 105 1235 714.6 0 1235 106 1632 714.6 0 1632 107 1844 714.6 0 1844 108 1155 714.6 0 1155 109 2046 714.6 0 2046 110 2101 714.6 0 2101 111 1832 714.6 0 1832 112 1472 714.6 0 1472 113 1852 714.6 0 1852 114 1374 714.6 0 1374 115 1285 714.6 0 1285 116 1015 714.6 0 1015 117 1097 714.6 0 1097 118 2086 714.6 0 2086 119 2225 714.6 0 2225 120 1291 714.6 0 1291 121 894 714.6 0 894 122 1317 714.6 0 1317 123 1601 714.6 0 1601 124 1679 714.6 0 1679 125 2073 714.6 0 2073 126 2190 714.6 0 2190 127 1973 714.6 0 1973 128 1445 714.6 0 1445 129 1146 714.6 0 1146 130 1194 714.6 0 1194 131 1297 714.6 0 1297 132 1665 714.6 0 1665 133 2982 714.6 0 2982 134 2158 714.6 0 2158 135 1368 714.6 0 1368 136 1338 714.6 0 1338 137 1417 714.6 0 1417 138 2546 714.6 0 2546 139 2874 714.6 0 2874 140 2277 714.6 0 2277 141 1309 714.6 0 1309 142 1021 714.6 0 1021 143 1429 714.6 0 1429 144 2552 714.6 0 2552 145 2371 714.6 0 2371 146 1956 714.6 0 1956 147 1602 714.6 0 1602 148 1251 714.6 0 1251 149 1813 714.6 0 1813 150 1576 714.6 0 1576 151 2333 714.6 0 2333 152 2572 714.6 0 2572 153 1933 714.6 0 1933 154 2020 714.6 0 2020 155 1438 714.6 0 1438 156 1896 714.6 0 1896 157 2485 714.6 0 2485 158 1739 714.6 0 1739 159 2164 714.6 0 2164 160 1681 714.6 0 1681 161 1584 714.6 0 1584 162 1334 714.6 0 1334 163 1325 714.6 0 1325 164 2244 714.6 0 2244 165 1924 714.6 0 1924 166 1478 714.6 0 1478 167 1161 714.6 0 1161 168 1197 714.6 0 1197 169 1148 714.6 0 1148 170 1214 714.6 0 1214 171 1397 714.6 0 1397 172 2238 714.6 0 2238 173 1803 714.6 0 1803 174 1244 714.6 0 1244 175 888 714.6 0 888 176 1161 714.6 0 1161 177 1310 714.6 0 1310 178 1163 714.6 0 1163 179 1860 714.6 0 1860 180 2174 714.6 0 2174 181 2087 714.6 0 2087 182 1352 714.6 0 1352 183 1157 714.6 0 1157 184 1483 714.6 0 1483 185 1893 714.6 0 1893 186 1599 714.6 0 1599 187 1300 714.6 0 1300 188 1581 714.6 0 1581 189 1952 714.6 0 1952 190 2103 714.6 0 2103 191 1445 714.6 0 1445 192 1234 714.6 0 1234 193 1171 714.6 0 1171 194 1395 714.6 0 1395 195 1038 714.6 0 1038 196 1544 714.6 0 1544 197 1794 714.6 0 1794 198 1019 714.6 0 1019 199 1821 714.6 0 1821 200 1598 714.6 0 1598 201 1918 714.6 0 1918 202 2123 714.6 0 2123 203 1269 714.6 0 1269 204 1238 714.6 0 1238 205 1732 714.6 0 1732 206 2265 714.6 0 2265 207 1913 714.6 0 1913 208 1701 714.6 0 1701 209 1547 714.6 0 1547 210 2010 714.6 0 2010 211 3462 714.6 0 3462 212 5054 714.6 0 5054 213 3699 714.6 0 3699 214 2623 714.6 0 2623 215 1553 714.6 0 1553 216 1418 714.6 0 1418 217 1333 714.6 0 1333 218 1785 714.6 0 1785 219 2190 714.6 0 2190 220 2225 714.6 0 2225 221 1411 714.6 0 1411 222 1589 714.6 0 1589 223 1798 714.6 0 1798 224 2114 714.6 0 2114 225 1856 714.6 0 1856 226 1606 714.6 0 1606 227 1330 714.6 0 1330 228 1349 714.6 0 1349 229 1529 714.6 0 1529 230 1589 714.6 0 1589 231 1671 714.6 0 1671 232 1421 714.6 0 1421 233 1725 714.6 0 1725 234 1879 714.6 0 1879 235 1556 714.6 0 1556 236 1684 714.6 0 1684 237 1494 714.6 0 1494 238 2430 714.6 0 2430 239 2169 714.6 0 2169 240 2064 714.6 0 2064 241 2310 714.6 0 2310 242 2151 714.6 0 2151 243 1728 714.6 0 1728 244 1368 714.6 0 1368 245 1854 714.6 0 1854 246 1652 714.6 0 1652 247 1577 714.6 0 1577 248 991 714.6 0 991 249 1784 714.6 0 1784 250 1969 714.6 0 1969 251 2500 714.6 0 2500 252 2475 714.6 0 2475 253 1289 714.6 0 1289 254 1416 714.6 0 1416 255 1583 714.6 0 1583 256 1567 714.6 0 1567 257 1540 714.6 0 1540 258 1329 714.6 0 1329 259 1641 714.6 0 1641 260 2069 714.6 0 2069 261 2411 714.6 0 2411 262 2395 714.6 0 2395 263 1615 714.6 0 1615 264 1449 714.6 0 1449 265 1697 714.6 0 1697 266 1704 714.6 0 1704 267 2360 714.6 0 2360 268 1454 714.6 0 1454 269 1740 714.6 0 1740 270 2529 714.6 0 2529 271 3316 714.6 0 3316 272 2971 714.6 0 2971 273 1605 714.6 0 1605 274 2153 714.6 0 2153 275 1632 714.6 0 1632 276 1786 714.6 0 1786 277 2947 714.6 0 2947 278 2450 714.6 0 2450 279 1909 714.6 0 1909 280 1492 714.6 0 1492 281 1458 714.6 0 1458 282 1964 714.6 0 1964 283 2357 714.6 0 2357 284 1784 714.6 0 1784 285 1390 714.6 0 1390 286 1917 714.6 0 1917 287 1682 714.6 0 1682 288 2138 714.6 0 2138 289 2517 714.6 0 2517 290 1673 714.6 0 1673 291 1997 714.6 0 1997 292 2703 714.6 0 2703 293 3450 714.6 0 3450 294 3937 714.6 0 3937 295 2258 714.6 0 2258 296 1912 714.6 0 1912 297 1803 714.6 0 1803 298 2109 714.6 0 2109 299 2372 714.6 0 2372 300 2639 714.6 0 2639 301 1158 714.6 0 1158 Finished in 9.74 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 285,655 Reads with adapters: 21,354 (7.5%) Reads that were too short: 96 (0.0%) Reads written (passing filters): 21,258 (7.4%) Total basepairs processed: 55,785,071 bp Total written (filtered): 5,831,118 bp (10.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 21354 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 12 0.0 3 0 0 12 16 226 0.0 3 0 214 7 5 17 20826 0.0 3 20185 551 67 23 18 47 0.0 3 1 42 1 3 19 19 0.0 3 1 1 7 10 20 22 0.0 3 1 0 0 21 21 2 0.0 3 0 0 0 2 22 3 0.0 3 0 0 0 3 23 2 0.0 3 0 0 0 2 25 2 0.0 3 0 0 0 2 26 2 0.0 3 0 0 0 2 27 4 0.0 3 0 0 0 4 29 1 0.0 3 0 0 0 1 30 2 0.0 3 0 0 0 2 32 2 0.0 3 0 0 0 2 33 2 0.0 3 0 0 0 2 34 2 0.0 3 1 0 0 1 35 1 0.0 3 1 36 3 0.0 3 0 0 0 3 39 1 0.0 3 0 0 0 1 41 2 0.0 3 0 0 0 2 42 1 0.0 3 0 0 0 1 44 3 0.0 3 0 0 0 3 45 3 0.0 3 0 0 1 2 46 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 50 3 0.0 3 0 0 1 2 53 3 0.0 3 0 0 0 3 55 8 0.0 3 0 0 0 8 56 1 0.0 3 0 0 1 58 5 0.0 3 0 0 1 4 59 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 69 3 0.0 3 0 0 0 3 71 2 0.0 3 0 0 0 2 72 3 0.0 3 0 0 0 3 75 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 83 2 0.0 3 0 0 0 2 84 1 0.0 3 0 0 0 1 85 2 0.0 3 0 0 0 2 87 2 0.0 3 0 0 0 2 90 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 92 2 0.0 3 0 0 0 2 93 3 0.0 3 0 0 0 3 95 3 0.0 3 0 0 0 3 96 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 114 3 0.0 3 0 0 0 3 115 2 0.0 3 0 0 0 2 118 2 0.0 3 0 0 0 2 119 2 0.0 3 0 0 0 2 120 2 0.0 3 0 0 0 2 122 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 132 2 0.0 3 0 0 0 2 133 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 151 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 5 0.0 3 0 0 0 5 167 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 199 2 0.0 3 0 0 0 2 217 1 0.0 3 0 0 0 1 218 3 0.0 3 0 0 0 3 221 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 237 2 0.0 3 0 0 0 2 239 1 0.0 3 0 0 0 1 270 3 0.0 3 0 0 0 3 280 1 0.0 3 0 0 0 1 281 4 0.0 3 0 0 0 4 282 13 0.0 3 0 0 0 13 283 2 0.0 3 0 0 0 2 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.75 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 21,258 Reads with adapters: 756 (3.6%) Reads written (passing filters): 21,258 (100.0%) Total basepairs processed: 5,831,118 bp Total written (filtered): 5,828,838 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 756 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 34.1% C: 10.4% G: 50.4% T: 5.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 745 332.2 0 745 4 10 83.0 0 10 5 1 20.8 0 1