This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTGCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.55 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 113,366 (3.9%) Reads that were too short: 50,557 (1.7%) Reads written (passing filters): 62,809 (2.1%) Total basepairs processed: 881,011,047 bp Total written (filtered): 15,874,498 bp (1.8%) === Adapter 1 === Sequence: CGTGCG; Type: regular 5'; Length: 6; Trimmed: 113366 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1778 714.6 0 1778 7 1232 714.6 0 1232 8 23360 714.6 0 23360 9 80 714.6 0 80 10 49 714.6 0 49 11 57 714.6 0 57 12 40 714.6 0 40 13 55 714.6 0 55 14 47 714.6 0 47 15 52 714.6 0 52 16 45 714.6 0 45 17 55 714.6 0 55 18 49 714.6 0 49 19 56 714.6 0 56 20 412 714.6 0 412 21 46 714.6 0 46 22 28 714.6 0 28 23 51 714.6 0 51 24 42 714.6 0 42 25 44 714.6 0 44 26 42 714.6 0 42 27 38 714.6 0 38 28 60 714.6 0 60 29 49 714.6 0 49 30 42 714.6 0 42 31 44 714.6 0 44 32 44 714.6 0 44 33 38 714.6 0 38 34 46 714.6 0 46 35 50 714.6 0 50 36 46 714.6 0 46 37 53 714.6 0 53 38 34 714.6 0 34 39 46 714.6 0 46 40 26 714.6 0 26 41 46 714.6 0 46 42 61 714.6 0 61 43 38 714.6 0 38 44 42 714.6 0 42 45 36 714.6 0 36 46 44 714.6 0 44 47 49 714.6 0 49 48 63 714.6 0 63 49 39 714.6 0 39 50 577 714.6 0 577 51 74 714.6 0 74 52 45 714.6 0 45 53 34 714.6 0 34 54 48 714.6 0 48 55 83 714.6 0 83 56 76 714.6 0 76 57 52 714.6 0 52 58 64 714.6 0 64 59 69 714.6 0 69 60 119 714.6 0 119 61 139 714.6 0 139 62 302 714.6 0 302 63 89 714.6 0 89 64 147 714.6 0 147 65 232 714.6 0 232 66 104 714.6 0 104 67 96 714.6 0 96 68 161 714.6 0 161 69 154 714.6 0 154 70 140 714.6 0 140 71 149 714.6 0 149 72 178 714.6 0 178 73 209 714.6 0 209 74 451 714.6 0 451 75 8460 714.6 0 8460 76 9652 714.6 0 9652 77 9082 714.6 0 9082 78 65 714.6 0 65 79 17 714.6 0 17 80 20 714.6 0 20 81 23 714.6 0 23 82 13 714.6 0 13 83 23 714.6 0 23 84 16 714.6 0 16 85 12 714.6 0 12 86 19 714.6 0 19 87 22 714.6 0 22 88 16 714.6 0 16 89 17 714.6 0 17 90 16 714.6 0 16 91 46 714.6 0 46 92 17 714.6 0 17 93 15 714.6 0 15 94 26 714.6 0 26 95 32 714.6 0 32 96 11 714.6 0 11 97 10 714.6 0 10 98 19 714.6 0 19 99 17 714.6 0 17 100 14 714.6 0 14 101 14 714.6 0 14 102 16 714.6 0 16 103 19 714.6 0 19 104 24 714.6 0 24 105 24 714.6 0 24 106 16 714.6 0 16 107 18 714.6 0 18 108 13 714.6 0 13 109 31 714.6 0 31 110 20 714.6 0 20 111 11 714.6 0 11 112 23 714.6 0 23 113 22 714.6 0 22 114 15 714.6 0 15 115 27 714.6 0 27 116 15 714.6 0 15 117 22 714.6 0 22 118 28 714.6 0 28 119 14 714.6 0 14 120 26 714.6 0 26 121 16 714.6 0 16 122 17 714.6 0 17 123 29 714.6 0 29 124 25 714.6 0 25 125 42 714.6 0 42 126 36 714.6 0 36 127 184 714.6 0 184 128 22 714.6 0 22 129 46 714.6 0 46 130 21 714.6 0 21 131 12 714.6 0 12 132 18 714.6 0 18 133 19 714.6 0 19 134 59 714.6 0 59 135 33 714.6 0 33 136 25 714.6 0 25 137 60 714.6 0 60 138 33 714.6 0 33 139 9 714.6 0 9 140 17 714.6 0 17 141 16 714.6 0 16 142 17 714.6 0 17 143 25 714.6 0 25 144 12 714.6 0 12 145 12 714.6 0 12 146 19 714.6 0 19 147 22 714.6 0 22 148 9 714.6 0 9 149 12 714.6 0 12 150 18 714.6 0 18 151 18 714.6 0 18 152 13 714.6 0 13 153 21 714.6 0 21 154 24 714.6 0 24 155 13 714.6 0 13 156 17 714.6 0 17 157 11 714.6 0 11 158 26 714.6 0 26 159 19 714.6 0 19 160 15 714.6 0 15 161 28 714.6 0 28 162 17 714.6 0 17 163 20 714.6 0 20 164 28 714.6 0 28 165 16 714.6 0 16 166 33 714.6 0 33 167 38 714.6 0 38 168 37 714.6 0 37 169 27 714.6 0 27 170 24 714.6 0 24 171 24 714.6 0 24 172 20 714.6 0 20 173 10 714.6 0 10 174 16 714.6 0 16 175 25 714.6 0 25 176 13 714.6 0 13 177 11 714.6 0 11 178 25 714.6 0 25 179 23 714.6 0 23 180 25 714.6 0 25 181 23 714.6 0 23 182 18 714.6 0 18 183 58 714.6 0 58 184 85 714.6 0 85 185 24 714.6 0 24 186 23 714.6 0 23 187 28 714.6 0 28 188 45 714.6 0 45 189 29 714.6 0 29 190 12 714.6 0 12 191 23 714.6 0 23 192 30 714.6 0 30 193 32 714.6 0 32 194 44 714.6 0 44 195 61 714.6 0 61 196 26 714.6 0 26 197 60 714.6 0 60 198 47 714.6 0 47 199 52 714.6 0 52 200 17 714.6 0 17 201 26 714.6 0 26 202 21 714.6 0 21 203 37 714.6 0 37 204 39 714.6 0 39 205 42 714.6 0 42 206 12 714.6 0 12 207 23 714.6 0 23 208 21 714.6 0 21 209 17 714.6 0 17 210 32 714.6 0 32 211 44 714.6 0 44 212 29 714.6 0 29 213 43 714.6 0 43 214 46 714.6 0 46 215 32 714.6 0 32 216 36 714.6 0 36 217 61 714.6 0 61 218 47 714.6 0 47 219 63 714.6 0 63 220 56 714.6 0 56 221 65 714.6 0 65 222 35 714.6 0 35 223 195 714.6 0 195 224 217 714.6 0 217 225 403 714.6 0 403 226 70 714.6 0 70 227 39 714.6 0 39 228 48 714.6 0 48 229 28 714.6 0 28 230 29 714.6 0 29 231 48 714.6 0 48 232 76 714.6 0 76 233 116 714.6 0 116 234 212 714.6 0 212 235 428 714.6 0 428 236 346 714.6 0 346 237 609 714.6 0 609 238 8662 714.6 0 8662 239 8303 714.6 0 8303 240 2706 714.6 0 2706 241 161 714.6 0 161 242 160 714.6 0 160 243 169 714.6 0 169 244 145 714.6 0 145 245 184 714.6 0 184 246 218 714.6 0 218 247 409 714.6 0 409 248 4390 714.6 0 4390 249 7413 714.6 0 7413 250 9379 714.6 0 9379 251 1645 714.6 0 1645 252 23 714.6 0 23 253 21 714.6 0 21 254 43 714.6 0 43 255 28 714.6 0 28 256 19 714.6 0 19 257 13 714.6 0 13 258 13 714.6 0 13 259 36 714.6 0 36 260 56 714.6 0 56 261 127 714.6 0 127 262 39 714.6 0 39 263 75 714.6 0 75 264 21 714.6 0 21 265 20 714.6 0 20 266 28 714.6 0 28 267 16 714.6 0 16 268 11 714.6 0 11 269 17 714.6 0 17 270 10 714.6 0 10 271 15 714.6 0 15 272 23 714.6 0 23 273 15 714.6 0 15 274 20 714.6 0 20 275 16 714.6 0 16 276 17 714.6 0 17 277 27 714.6 0 27 278 32 714.6 0 32 279 54 714.6 0 54 280 135 714.6 0 135 281 51 714.6 0 51 282 203 714.6 0 203 283 428 714.6 0 428 284 414 714.6 0 414 285 30 714.6 0 30 286 37 714.6 0 37 287 34 714.6 0 34 288 16 714.6 0 16 289 14 714.6 0 14 290 14 714.6 0 14 291 18 714.6 0 18 292 34 714.6 0 34 293 20 714.6 0 20 294 62 714.6 0 62 295 127 714.6 0 127 296 116 714.6 0 116 297 134 714.6 0 134 298 20 714.6 0 20 299 38 714.6 0 38 300 69 714.6 0 69 301 99 714.6 0 99 Finished in 3.03 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 62,809 Reads with adapters: 26,128 (41.6%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 26,125 (41.6%) Total basepairs processed: 15,874,498 bp Total written (filtered): 7,133,457 bp (44.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 26128 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 5 0.0 3 0 0 0 1 4 18 17 0.0 3 0 0 16 1 19 367 0.0 3 0 361 6 20 24286 0.0 4 23972 303 11 21 90 0.0 4 17 70 1 0 2 22 1337 0.0 4 1319 14 4 23 10 0.0 4 2 2 0 5 1 24 2 0.0 4 0 0 1 0 1 25 1 0.0 4 0 0 0 1 37 1 0.0 4 1 44 1 0.0 4 1 59 3 0.0 4 0 0 0 0 3 61 1 0.0 4 1 84 1 0.0 4 0 0 0 0 1 89 1 0.0 4 1 111 2 0.0 4 0 0 0 1 1 159 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.90 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 26,125 Reads with adapters: 1,651 (6.3%) Reads written (passing filters): 26,125 (100.0%) Total basepairs processed: 7,133,457 bp Total written (filtered): 7,022,315 bp (98.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1651 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 17.9% C: 0.8% G: 75.2% T: 6.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 408.2 0 1 4 2 102.1 0 2 5 2 25.5 0 2 9 1 0.1 0 0 1 10 39 0.0 1 1 38 11 1219 0.0 1 3 1216 210 1 0.0 1 1 224 85 0.0 1 76 9 225 5 0.0 1 5 226 1 0.0 1 1 258 1 0.0 1 1 259 189 0.0 1 163 26 260 25 0.0 1 21 4 261 3 0.0 1 3 262 67 0.0 1 60 7 263 5 0.0 1 2 3 264 1 0.0 1 1 271 1 0.0 1 1 272 3 0.0 1 3