This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.85 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 236,463 (8.1%) Reads that were too short: 122,943 (4.2%) Reads written (passing filters): 113,520 (3.9%) Total basepairs processed: 881,011,047 bp Total written (filtered): 23,100,520 bp (2.6%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 236463 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 854 714.6 0 854 7 644 714.6 0 644 8 35422 714.6 0 35422 9 4641 714.6 0 4641 10 67 714.6 0 67 11 40 714.6 0 40 12 38 714.6 0 38 13 36 714.6 0 36 14 40 714.6 0 40 15 29 714.6 0 29 16 30 714.6 0 30 17 41 714.6 0 41 18 45 714.6 0 45 19 38 714.6 0 38 20 33 714.6 0 33 21 39 714.6 0 39 22 23 714.6 0 23 23 30 714.6 0 30 24 22 714.6 0 22 25 26 714.6 0 26 26 25 714.6 0 25 27 18 714.6 0 18 28 40 714.6 0 40 29 28 714.6 0 28 30 45 714.6 0 45 31 25 714.6 0 25 32 38 714.6 0 38 33 31 714.6 0 31 34 19 714.6 0 19 35 25 714.6 0 25 36 28 714.6 0 28 37 51 714.6 0 51 38 92 714.6 0 92 39 63 714.6 0 63 40 28 714.6 0 28 41 44 714.6 0 44 42 37 714.6 0 37 43 35 714.6 0 35 44 281 714.6 0 281 45 49 714.6 0 49 46 31 714.6 0 31 47 35 714.6 0 35 48 56 714.6 0 56 49 43 714.6 0 43 50 42 714.6 0 42 51 87 714.6 0 87 52 31 714.6 0 31 53 41 714.6 0 41 54 136 714.6 0 136 55 37 714.6 0 37 56 117 714.6 0 117 57 28 714.6 0 28 58 46 714.6 0 46 59 31 714.6 0 31 60 24 714.6 0 24 61 26 714.6 0 26 62 44 714.6 0 44 63 25 714.6 0 25 64 42 714.6 0 42 65 34 714.6 0 34 66 36 714.6 0 36 67 30 714.6 0 30 68 43 714.6 0 43 69 21 714.6 0 21 70 17 714.6 0 17 71 111 714.6 0 111 72 59 714.6 0 59 73 770 714.6 0 770 74 408 714.6 0 408 75 442 714.6 0 442 76 73 714.6 0 73 77 165 714.6 0 165 78 185 714.6 0 185 79 140 714.6 0 140 80 70 714.6 0 70 81 164 714.6 0 164 82 228 714.6 0 228 83 196 714.6 0 196 84 209 714.6 0 209 85 339 714.6 0 339 86 318 714.6 0 318 87 198 714.6 0 198 88 208 714.6 0 208 89 113 714.6 0 113 90 197 714.6 0 197 91 261 714.6 0 261 92 200 714.6 0 200 93 330 714.6 0 330 94 566 714.6 0 566 95 378 714.6 0 378 96 327 714.6 0 327 97 373 714.6 0 373 98 386 714.6 0 386 99 373 714.6 0 373 100 111 714.6 0 111 101 77 714.6 0 77 102 97 714.6 0 97 103 125 714.6 0 125 104 74 714.6 0 74 105 59 714.6 0 59 106 107 714.6 0 107 107 198 714.6 0 198 108 206 714.6 0 206 109 188 714.6 0 188 110 187 714.6 0 187 111 163 714.6 0 163 112 198 714.6 0 198 113 217 714.6 0 217 114 294 714.6 0 294 115 470 714.6 0 470 116 339 714.6 0 339 117 261 714.6 0 261 118 237 714.6 0 237 119 389 714.6 0 389 120 461 714.6 0 461 121 391 714.6 0 391 122 279 714.6 0 279 123 268 714.6 0 268 124 345 714.6 0 345 125 493 714.6 0 493 126 505 714.6 0 505 127 628 714.6 0 628 128 496 714.6 0 496 129 501 714.6 0 501 130 423 714.6 0 423 131 335 714.6 0 335 132 253 714.6 0 253 133 375 714.6 0 375 134 783 714.6 0 783 135 855 714.6 0 855 136 751 714.6 0 751 137 710 714.6 0 710 138 408 714.6 0 408 139 342 714.6 0 342 140 366 714.6 0 366 141 491 714.6 0 491 142 340 714.6 0 340 143 347 714.6 0 347 144 339 714.6 0 339 145 1032 714.6 0 1032 146 1046 714.6 0 1046 147 952 714.6 0 952 148 525 714.6 0 525 149 426 714.6 0 426 150 391 714.6 0 391 151 500 714.6 0 500 152 513 714.6 0 513 153 670 714.6 0 670 154 781 714.6 0 781 155 764 714.6 0 764 156 695 714.6 0 695 157 756 714.6 0 756 158 674 714.6 0 674 159 563 714.6 0 563 160 576 714.6 0 576 161 639 714.6 0 639 162 517 714.6 0 517 163 447 714.6 0 447 164 615 714.6 0 615 165 1182 714.6 0 1182 166 1096 714.6 0 1096 167 659 714.6 0 659 168 460 714.6 0 460 169 449 714.6 0 449 170 425 714.6 0 425 171 458 714.6 0 458 172 498 714.6 0 498 173 2044 714.6 0 2044 174 2932 714.6 0 2932 175 1623 714.6 0 1623 176 856 714.6 0 856 177 470 714.6 0 470 178 451 714.6 0 451 179 636 714.6 0 636 180 761 714.6 0 761 181 2690 714.6 0 2690 182 1139 714.6 0 1139 183 1159 714.6 0 1159 184 974 714.6 0 974 185 794 714.6 0 794 186 1238 714.6 0 1238 187 797 714.6 0 797 188 473 714.6 0 473 189 473 714.6 0 473 190 646 714.6 0 646 191 791 714.6 0 791 192 1077 714.6 0 1077 193 1397 714.6 0 1397 194 677 714.6 0 677 195 329 714.6 0 329 196 374 714.6 0 374 197 508 714.6 0 508 198 811 714.6 0 811 199 613 714.6 0 613 200 464 714.6 0 464 201 502 714.6 0 502 202 684 714.6 0 684 203 728 714.6 0 728 204 866 714.6 0 866 205 639 714.6 0 639 206 703 714.6 0 703 207 781 714.6 0 781 208 592 714.6 0 592 209 545 714.6 0 545 210 512 714.6 0 512 211 646 714.6 0 646 212 777 714.6 0 777 213 690 714.6 0 690 214 823 714.6 0 823 215 791 714.6 0 791 216 705 714.6 0 705 217 822 714.6 0 822 218 891 714.6 0 891 219 1190 714.6 0 1190 220 932 714.6 0 932 221 1009 714.6 0 1009 222 796 714.6 0 796 223 887 714.6 0 887 224 732 714.6 0 732 225 3491 714.6 0 3491 226 4637 714.6 0 4637 227 7042 714.6 0 7042 228 773 714.6 0 773 229 623 714.6 0 623 230 1288 714.6 0 1288 231 2163 714.6 0 2163 232 2460 714.6 0 2460 233 1239 714.6 0 1239 234 2146 714.6 0 2146 235 981 714.6 0 981 236 601 714.6 0 601 237 666 714.6 0 666 238 779 714.6 0 779 239 761 714.6 0 761 240 477 714.6 0 477 241 759 714.6 0 759 242 874 714.6 0 874 243 733 714.6 0 733 244 698 714.6 0 698 245 661 714.6 0 661 246 1335 714.6 0 1335 247 1647 714.6 0 1647 248 1252 714.6 0 1252 249 285 714.6 0 285 250 1113 714.6 0 1113 251 2392 714.6 0 2392 252 3860 714.6 0 3860 253 1959 714.6 0 1959 254 1512 714.6 0 1512 255 1130 714.6 0 1130 256 1081 714.6 0 1081 257 789 714.6 0 789 258 558 714.6 0 558 259 283 714.6 0 283 260 351 714.6 0 351 261 399 714.6 0 399 262 423 714.6 0 423 263 520 714.6 0 520 264 523 714.6 0 523 265 686 714.6 0 686 266 1100 714.6 0 1100 267 965 714.6 0 965 268 667 714.6 0 667 269 471 714.6 0 471 270 640 714.6 0 640 271 840 714.6 0 840 272 938 714.6 0 938 273 812 714.6 0 812 274 656 714.6 0 656 275 454 714.6 0 454 276 459 714.6 0 459 277 738 714.6 0 738 278 1070 714.6 0 1070 279 1376 714.6 0 1376 280 1928 714.6 0 1928 281 1604 714.6 0 1604 282 1076 714.6 0 1076 283 673 714.6 0 673 284 773 714.6 0 773 285 1244 714.6 0 1244 286 995 714.6 0 995 287 620 714.6 0 620 288 524 714.6 0 524 289 549 714.6 0 549 290 845 714.6 0 845 291 1147 714.6 0 1147 292 1167 714.6 0 1167 293 657 714.6 0 657 294 689 714.6 0 689 295 837 714.6 0 837 296 811 714.6 0 811 297 823 714.6 0 823 298 1198 714.6 0 1198 299 1740 714.6 0 1740 300 2265 714.6 0 2265 301 15901 714.6 0 15901 Finished in 4.50 s (40 us/read; 1.51 M reads/minute). === Summary === Total reads processed: 113,520 Reads with adapters: 36,692 (32.3%) Reads that were too short: 44 (0.0%) Reads written (passing filters): 36,648 (32.3%) Total basepairs processed: 23,100,520 bp Total written (filtered): 10,109,374 bp (43.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 36692 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 9 0.0 3 0 0 6 3 16 289 0.0 3 0 254 18 17 17 35955 0.0 3 34716 1041 151 47 18 71 0.0 3 5 58 5 3 19 69 0.0 3 0 3 47 19 20 207 0.0 3 177 5 0 25 21 1 0.0 3 0 0 0 1 23 1 0.0 3 1 28 1 0.0 3 0 0 0 1 31 1 0.0 3 1 32 3 0.0 3 0 0 0 3 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 41 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 45 5 0.0 3 0 0 0 5 46 7 0.0 3 0 0 0 7 48 1 0.0 3 0 0 0 1 49 2 0.0 3 0 0 0 2 51 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 66 3 0.0 3 0 0 0 3 68 1 0.0 3 0 0 0 1 69 3 0.0 3 0 0 0 3 75 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 97 3 0.0 3 0 0 0 3 99 2 0.0 3 0 0 0 2 100 3 0.0 3 0 0 0 3 101 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 116 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 118 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 1 141 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 187 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.28 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 36,648 Reads with adapters: 244 (0.7%) Reads written (passing filters): 36,648 (100.0%) Total basepairs processed: 10,109,374 bp Total written (filtered): 10,108,633 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 244 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 43.4% C: 12.3% G: 33.6% T: 10.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 237 572.6 0 237 4 5 143.2 0 5 5 2 35.8 0 2