This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.88 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 781,420 (26.7%) Reads that were too short: 286,046 (9.8%) Reads written (passing filters): 495,374 (16.9%) Total basepairs processed: 881,011,047 bp Total written (filtered): 84,241,589 bp (9.6%) === Adapter 1 === Sequence: AAGACA; Type: regular 5'; Length: 6; Trimmed: 781420 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 931 714.6 0 931 7 36992 714.6 0 36992 8 58 714.6 0 58 9 39 714.6 0 39 10 9 714.6 0 9 11 100 714.6 0 100 12 53 714.6 0 53 13 18 714.6 0 18 14 19 714.6 0 19 15 18 714.6 0 18 16 12 714.6 0 12 17 12 714.6 0 12 18 12 714.6 0 12 19 23 714.6 0 23 20 28 714.6 0 28 21 13 714.6 0 13 22 9 714.6 0 9 23 20 714.6 0 20 24 17 714.6 0 17 25 14 714.6 0 14 26 21 714.6 0 21 27 16 714.6 0 16 28 14 714.6 0 14 29 34 714.6 0 34 30 22 714.6 0 22 31 12 714.6 0 12 32 19 714.6 0 19 33 27 714.6 0 27 34 18 714.6 0 18 35 14 714.6 0 14 36 18 714.6 0 18 37 18 714.6 0 18 38 32 714.6 0 32 39 22 714.6 0 22 40 12 714.6 0 12 41 25 714.6 0 25 42 12 714.6 0 12 43 14 714.6 0 14 44 23 714.6 0 23 45 20 714.6 0 20 46 15 714.6 0 15 47 21 714.6 0 21 48 7 714.6 0 7 49 25 714.6 0 25 50 28 714.6 0 28 51 16 714.6 0 16 52 15 714.6 0 15 53 18 714.6 0 18 54 22 714.6 0 22 55 29 714.6 0 29 56 25 714.6 0 25 57 130 714.6 0 130 58 18 714.6 0 18 59 13 714.6 0 13 60 24 714.6 0 24 61 21 714.6 0 21 62 19 714.6 0 19 63 21 714.6 0 21 64 16 714.6 0 16 65 31 714.6 0 31 66 10 714.6 0 10 67 13 714.6 0 13 68 13 714.6 0 13 69 22 714.6 0 22 70 30 714.6 0 30 71 42 714.6 0 42 72 67 714.6 0 67 73 19 714.6 0 19 74 71 714.6 0 71 75 44 714.6 0 44 76 86 714.6 0 86 77 446 714.6 0 446 78 1119 714.6 0 1119 79 2235 714.6 0 2235 80 5191 714.6 0 5191 81 4110 714.6 0 4110 82 4623 714.6 0 4623 83 5847 714.6 0 5847 84 4994 714.6 0 4994 85 4297 714.6 0 4297 86 5562 714.6 0 5562 87 5702 714.6 0 5702 88 2829 714.6 0 2829 89 1495 714.6 0 1495 90 1509 714.6 0 1509 91 2703 714.6 0 2703 92 6023 714.6 0 6023 93 2688 714.6 0 2688 94 3886 714.6 0 3886 95 2954 714.6 0 2954 96 2071 714.6 0 2071 97 1821 714.6 0 1821 98 1535 714.6 0 1535 99 1419 714.6 0 1419 100 4497 714.6 0 4497 101 5442 714.6 0 5442 102 3471 714.6 0 3471 103 3072 714.6 0 3072 104 2138 714.6 0 2138 105 1475 714.6 0 1475 106 1170 714.6 0 1170 107 1009 714.6 0 1009 108 2623 714.6 0 2623 109 3597 714.6 0 3597 110 4066 714.6 0 4066 111 3229 714.6 0 3229 112 3729 714.6 0 3729 113 2873 714.6 0 2873 114 2376 714.6 0 2376 115 2731 714.6 0 2731 116 4396 714.6 0 4396 117 4865 714.6 0 4865 118 5308 714.6 0 5308 119 4667 714.6 0 4667 120 4762 714.6 0 4762 121 4613 714.6 0 4613 122 4504 714.6 0 4504 123 3569 714.6 0 3569 124 3401 714.6 0 3401 125 3780 714.6 0 3780 126 3166 714.6 0 3166 127 2509 714.6 0 2509 128 2883 714.6 0 2883 129 3638 714.6 0 3638 130 3272 714.6 0 3272 131 5265 714.6 0 5265 132 3578 714.6 0 3578 133 2902 714.6 0 2902 134 2831 714.6 0 2831 135 3008 714.6 0 3008 136 3461 714.6 0 3461 137 3820 714.6 0 3820 138 4458 714.6 0 4458 139 4321 714.6 0 4321 140 3273 714.6 0 3273 141 3145 714.6 0 3145 142 4044 714.6 0 4044 143 3684 714.6 0 3684 144 3282 714.6 0 3282 145 4162 714.6 0 4162 146 4061 714.6 0 4061 147 3943 714.6 0 3943 148 3662 714.6 0 3662 149 3035 714.6 0 3035 150 3645 714.6 0 3645 151 3386 714.6 0 3386 152 4088 714.6 0 4088 153 3967 714.6 0 3967 154 3267 714.6 0 3267 155 3575 714.6 0 3575 156 3128 714.6 0 3128 157 3420 714.6 0 3420 158 3674 714.6 0 3674 159 3552 714.6 0 3552 160 3655 714.6 0 3655 161 2994 714.6 0 2994 162 3262 714.6 0 3262 163 2850 714.6 0 2850 164 2872 714.6 0 2872 165 2314 714.6 0 2314 166 4324 714.6 0 4324 167 5264 714.6 0 5264 168 4184 714.6 0 4184 169 3354 714.6 0 3354 170 4111 714.6 0 4111 171 4912 714.6 0 4912 172 4352 714.6 0 4352 173 2916 714.6 0 2916 174 2711 714.6 0 2711 175 3796 714.6 0 3796 176 3379 714.6 0 3379 177 3727 714.6 0 3727 178 3875 714.6 0 3875 179 3215 714.6 0 3215 180 4288 714.6 0 4288 181 4565 714.6 0 4565 182 21054 714.6 0 21054 183 5564 714.6 0 5564 184 4342 714.6 0 4342 185 4350 714.6 0 4350 186 3065 714.6 0 3065 187 3542 714.6 0 3542 188 3603 714.6 0 3603 189 3178 714.6 0 3178 190 2939 714.6 0 2939 191 3091 714.6 0 3091 192 2914 714.6 0 2914 193 2820 714.6 0 2820 194 2705 714.6 0 2705 195 2913 714.6 0 2913 196 2664 714.6 0 2664 197 3726 714.6 0 3726 198 4607 714.6 0 4607 199 3548 714.6 0 3548 200 4200 714.6 0 4200 201 2381 714.6 0 2381 202 2654 714.6 0 2654 203 3256 714.6 0 3256 204 3268 714.6 0 3268 205 3336 714.6 0 3336 206 3147 714.6 0 3147 207 2898 714.6 0 2898 208 2639 714.6 0 2639 209 2397 714.6 0 2397 210 2749 714.6 0 2749 211 2859 714.6 0 2859 212 2872 714.6 0 2872 213 4525 714.6 0 4525 214 4003 714.6 0 4003 215 3146 714.6 0 3146 216 2723 714.6 0 2723 217 2758 714.6 0 2758 218 3653 714.6 0 3653 219 3844 714.6 0 3844 220 2737 714.6 0 2737 221 2254 714.6 0 2254 222 2566 714.6 0 2566 223 3107 714.6 0 3107 224 3090 714.6 0 3090 225 2261 714.6 0 2261 226 2370 714.6 0 2370 227 2339 714.6 0 2339 228 3006 714.6 0 3006 229 2801 714.6 0 2801 230 2310 714.6 0 2310 231 2294 714.6 0 2294 232 2612 714.6 0 2612 233 2574 714.6 0 2574 234 2398 714.6 0 2398 235 2560 714.6 0 2560 236 2069 714.6 0 2069 237 2387 714.6 0 2387 238 2608 714.6 0 2608 239 3132 714.6 0 3132 240 2637 714.6 0 2637 241 2356 714.6 0 2356 242 2626 714.6 0 2626 243 2391 714.6 0 2391 244 2465 714.6 0 2465 245 2358 714.6 0 2358 246 2824 714.6 0 2824 247 2530 714.6 0 2530 248 2724 714.6 0 2724 249 2195 714.6 0 2195 250 2953 714.6 0 2953 251 2736 714.6 0 2736 252 3841 714.6 0 3841 253 2757 714.6 0 2757 254 2644 714.6 0 2644 255 2246 714.6 0 2246 256 2227 714.6 0 2227 257 2136 714.6 0 2136 258 2001 714.6 0 2001 259 1800 714.6 0 1800 260 2001 714.6 0 2001 261 2322 714.6 0 2322 262 3619 714.6 0 3619 263 3496 714.6 0 3496 264 3022 714.6 0 3022 265 2207 714.6 0 2207 266 2452 714.6 0 2452 267 9068 714.6 0 9068 268 7422 714.6 0 7422 269 8971 714.6 0 8971 270 2683 714.6 0 2683 271 2231 714.6 0 2231 272 3225 714.6 0 3225 273 3039 714.6 0 3039 274 2161 714.6 0 2161 275 1888 714.6 0 1888 276 1815 714.6 0 1815 277 2262 714.6 0 2262 278 2574 714.6 0 2574 279 2597 714.6 0 2597 280 1790 714.6 0 1790 281 2529 714.6 0 2529 282 1972 714.6 0 1972 283 2275 714.6 0 2275 284 2148 714.6 0 2148 285 1709 714.6 0 1709 286 2141 714.6 0 2141 287 2015 714.6 0 2015 288 1854 714.6 0 1854 289 1786 714.6 0 1786 290 2591 714.6 0 2591 291 3344 714.6 0 3344 292 3721 714.6 0 3721 293 2615 714.6 0 2615 294 2221 714.6 0 2221 295 1953 714.6 0 1953 296 2382 714.6 0 2382 297 3247 714.6 0 3247 298 3847 714.6 0 3847 299 4474 714.6 0 4474 300 4035 714.6 0 4035 301 3693 714.6 0 3693 Finished in 17.33 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 495,374 Reads with adapters: 38,091 (7.7%) Reads that were too short: 153 (0.0%) Reads written (passing filters): 37,938 (7.7%) Total basepairs processed: 84,241,589 bp Total written (filtered): 10,389,902 bp (12.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 38091 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 6 0.0 3 0 0 0 0 6 18 18 0.0 3 0 0 17 0 1 19 358 0.0 3 0 351 5 2 20 37392 0.0 4 36905 470 14 2 1 21 101 0.0 4 11 88 0 0 2 22 8 0.0 4 0 0 8 23 7 0.0 4 0 0 0 7 24 2 0.0 4 0 0 0 0 2 25 2 0.0 4 2 26 2 0.0 4 1 0 0 0 1 27 2 0.0 4 1 0 0 0 1 28 1 0.0 4 1 29 1 0.0 4 0 0 0 0 1 30 3 0.0 4 1 0 0 0 2 32 3 0.0 4 1 0 0 0 2 33 2 0.0 4 0 0 0 0 2 35 1 0.0 4 0 0 0 0 1 39 2 0.0 4 0 0 0 0 2 40 3 0.0 4 0 0 0 0 3 41 1 0.0 4 0 0 0 0 1 42 2 0.0 4 0 0 0 0 2 43 1 0.0 4 0 0 0 1 45 2 0.0 4 0 0 0 0 2 46 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 48 7 0.0 4 0 0 0 0 7 50 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 52 2 0.0 4 0 0 0 0 2 53 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 59 2 0.0 4 0 0 0 0 2 60 2 0.0 4 0 0 0 1 1 61 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 64 2 0.0 4 0 0 0 0 2 65 2 0.0 4 0 0 0 0 2 67 1 0.0 4 0 0 0 0 1 68 2 0.0 4 0 0 0 0 2 69 2 0.0 4 0 0 0 0 2 70 1 0.0 4 0 0 0 0 1 71 3 0.0 4 0 0 0 1 2 73 2 0.0 4 0 0 0 0 2 74 1 0.0 4 0 0 0 0 1 76 2 0.0 4 0 0 0 0 2 80 3 0.0 4 0 0 0 0 3 81 2 0.0 4 0 0 0 0 2 82 1 0.0 4 0 0 0 0 1 83 2 0.0 4 0 0 0 1 1 84 2 0.0 4 0 0 0 0 2 85 2 0.0 4 0 0 0 0 2 86 2 0.0 4 0 0 0 0 2 89 2 0.0 4 0 0 0 0 2 90 2 0.0 4 0 0 0 0 2 91 1 0.0 4 0 0 0 0 1 92 3 0.0 4 0 0 0 0 3 93 1 0.0 4 0 0 0 0 1 94 2 0.0 4 0 0 0 0 2 96 4 0.0 4 0 0 0 0 4 97 2 0.0 4 0 0 0 0 2 98 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 100 2 0.0 4 0 0 0 0 2 101 2 0.0 4 0 0 0 0 2 102 2 0.0 4 0 0 0 0 2 103 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 106 2 0.0 4 0 0 0 0 2 107 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 111 2 0.0 4 0 0 0 0 2 112 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 114 3 0.0 4 0 0 0 0 3 116 1 0.0 4 0 0 0 0 1 117 3 0.0 4 0 0 0 0 3 118 1 0.0 4 0 0 0 0 1 119 2 0.0 4 0 0 0 0 2 120 4 0.0 4 0 0 0 0 4 121 1 0.0 4 0 0 0 0 1 122 2 0.0 4 0 0 0 0 2 125 2 0.0 4 0 0 0 0 2 126 1 0.0 4 0 0 0 0 1 128 2 0.0 4 0 0 0 0 2 132 1 0.0 4 0 0 0 0 1 133 4 0.0 4 0 0 0 0 4 135 2 0.0 4 0 0 0 0 2 137 2 0.0 4 0 0 0 0 2 138 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 140 3 0.0 4 0 0 0 1 2 141 3 0.0 4 0 0 0 0 3 142 2 0.0 4 0 0 0 0 2 144 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 147 3 0.0 4 0 0 0 0 3 148 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 155 2 0.0 4 0 0 0 0 2 156 1 0.0 4 0 0 0 0 1 157 3 0.0 4 0 0 0 0 3 158 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 160 2 0.0 4 0 0 0 0 2 161 1 0.0 4 0 0 0 0 1 162 2 0.0 4 0 0 0 0 2 163 1 0.0 4 0 0 0 1 164 3 0.0 4 0 0 0 0 3 166 1 0.0 4 0 0 0 0 1 167 2 0.0 4 0 0 0 0 2 169 1 0.0 4 0 0 0 0 1 171 2 0.0 4 0 0 0 0 2 174 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.30 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 37,938 Reads with adapters: 447 (1.2%) Reads written (passing filters): 37,938 (100.0%) Total basepairs processed: 10,389,902 bp Total written (filtered): 10,367,667 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 447 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 19.2% C: 1.1% G: 78.7% T: 0.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 10 592.8 0 10 4 4 148.2 0 4 5 3 37.0 0 3 6 1 9.3 0 1 9 1 0.1 0 0 1 10 2 0.0 1 0 2 11 313 0.0 1 0 313 12 34 0.0 1 0 34 14 2 0.0 1 0 2 160 1 0.0 1 0 1 180 14 0.0 1 11 3 181 1 0.0 1 1 185 1 0.0 1 1 207 7 0.0 1 5 2 210 1 0.0 1 1 234 3 0.0 1 3 261 17 0.0 1 17 262 1 0.0 1 1 263 30 0.0 1 30 264 1 0.0 1 1