This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 77.32 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 68,798 (2.4%) Reads that were too short: 32,744 (1.1%) Reads written (passing filters): 36,054 (1.2%) Total basepairs processed: 881,011,047 bp Total written (filtered): 7,153,557 bp (0.8%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 68798 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 111 714.6 0 111 7 2009 714.6 0 2009 8 6342 714.6 0 6342 9 41 714.6 0 41 10 30 714.6 0 30 11 33 714.6 0 33 12 27 714.6 0 27 13 30 714.6 0 30 14 35 714.6 0 35 15 26 714.6 0 26 16 26 714.6 0 26 17 29 714.6 0 29 18 33 714.6 0 33 19 26 714.6 0 26 20 74 714.6 0 74 21 67 714.6 0 67 22 63 714.6 0 63 23 369 714.6 0 369 24 23 714.6 0 23 25 35 714.6 0 35 26 36 714.6 0 36 27 31 714.6 0 31 28 31 714.6 0 31 29 24 714.6 0 24 30 32 714.6 0 32 31 253 714.6 0 253 32 60 714.6 0 60 33 58 714.6 0 58 34 73 714.6 0 73 35 53 714.6 0 53 36 55 714.6 0 55 37 34 714.6 0 34 38 37 714.6 0 37 39 74 714.6 0 74 40 64 714.6 0 64 41 90 714.6 0 90 42 28 714.6 0 28 43 87 714.6 0 87 44 28 714.6 0 28 45 25 714.6 0 25 46 34 714.6 0 34 47 29 714.6 0 29 48 27 714.6 0 27 49 22 714.6 0 22 50 21 714.6 0 21 51 31 714.6 0 31 52 59 714.6 0 59 53 46 714.6 0 46 54 37 714.6 0 37 55 34 714.6 0 34 56 29 714.6 0 29 57 75 714.6 0 75 58 37 714.6 0 37 59 29 714.6 0 29 60 54 714.6 0 54 61 43 714.6 0 43 62 26 714.6 0 26 63 23 714.6 0 23 64 37 714.6 0 37 65 49 714.6 0 49 66 27 714.6 0 27 67 28 714.6 0 28 68 32 714.6 0 32 69 24 714.6 0 24 70 50 714.6 0 50 71 36 714.6 0 36 72 30 714.6 0 30 73 40 714.6 0 40 74 53 714.6 0 53 75 45 714.6 0 45 76 56 714.6 0 56 77 58 714.6 0 58 78 95 714.6 0 95 79 80 714.6 0 80 80 112 714.6 0 112 81 123 714.6 0 123 82 154 714.6 0 154 83 114 714.6 0 114 84 105 714.6 0 105 85 230 714.6 0 230 86 341 714.6 0 341 87 130 714.6 0 130 88 67 714.6 0 67 89 94 714.6 0 94 90 66 714.6 0 66 91 157 714.6 0 157 92 321 714.6 0 321 93 174 714.6 0 174 94 144 714.6 0 144 95 166 714.6 0 166 96 135 714.6 0 135 97 149 714.6 0 149 98 181 714.6 0 181 99 229 714.6 0 229 100 314 714.6 0 314 101 222 714.6 0 222 102 240 714.6 0 240 103 334 714.6 0 334 104 203 714.6 0 203 105 58 714.6 0 58 106 37 714.6 0 37 107 36 714.6 0 36 108 27 714.6 0 27 109 72 714.6 0 72 110 118 714.6 0 118 111 197 714.6 0 197 112 142 714.6 0 142 113 161 714.6 0 161 114 111 714.6 0 111 115 136 714.6 0 136 116 219 714.6 0 219 117 208 714.6 0 208 118 221 714.6 0 221 119 164 714.6 0 164 120 103 714.6 0 103 121 81 714.6 0 81 122 100 714.6 0 100 123 255 714.6 0 255 124 351 714.6 0 351 125 271 714.6 0 271 126 138 714.6 0 138 127 113 714.6 0 113 128 119 714.6 0 119 129 120 714.6 0 120 130 147 714.6 0 147 131 204 714.6 0 204 132 284 714.6 0 284 133 337 714.6 0 337 134 159 714.6 0 159 135 166 714.6 0 166 136 152 714.6 0 152 137 242 714.6 0 242 138 356 714.6 0 356 139 287 714.6 0 287 140 175 714.6 0 175 141 141 714.6 0 141 142 321 714.6 0 321 143 501 714.6 0 501 144 543 714.6 0 543 145 433 714.6 0 433 146 156 714.6 0 156 147 121 714.6 0 121 148 125 714.6 0 125 149 160 714.6 0 160 150 183 714.6 0 183 151 157 714.6 0 157 152 185 714.6 0 185 153 148 714.6 0 148 154 150 714.6 0 150 155 114 714.6 0 114 156 145 714.6 0 145 157 163 714.6 0 163 158 265 714.6 0 265 159 237 714.6 0 237 160 160 714.6 0 160 161 147 714.6 0 147 162 255 714.6 0 255 163 178 714.6 0 178 164 173 714.6 0 173 165 138 714.6 0 138 166 152 714.6 0 152 167 216 714.6 0 216 168 234 714.6 0 234 169 276 714.6 0 276 170 372 714.6 0 372 171 399 714.6 0 399 172 297 714.6 0 297 173 140 714.6 0 140 174 117 714.6 0 117 175 86 714.6 0 86 176 97 714.6 0 97 177 282 714.6 0 282 178 348 714.6 0 348 179 323 714.6 0 323 180 205 714.6 0 205 181 152 714.6 0 152 182 133 714.6 0 133 183 231 714.6 0 231 184 331 714.6 0 331 185 256 714.6 0 256 186 193 714.6 0 193 187 375 714.6 0 375 188 319 714.6 0 319 189 323 714.6 0 323 190 161 714.6 0 161 191 140 714.6 0 140 192 168 714.6 0 168 193 201 714.6 0 201 194 272 714.6 0 272 195 160 714.6 0 160 196 152 714.6 0 152 197 183 714.6 0 183 198 351 714.6 0 351 199 326 714.6 0 326 200 140 714.6 0 140 201 74 714.6 0 74 202 94 714.6 0 94 203 100 714.6 0 100 204 200 714.6 0 200 205 219 714.6 0 219 206 225 714.6 0 225 207 196 714.6 0 196 208 151 714.6 0 151 209 139 714.6 0 139 210 274 714.6 0 274 211 480 714.6 0 480 212 335 714.6 0 335 213 277 714.6 0 277 214 695 714.6 0 695 215 852 714.6 0 852 216 639 714.6 0 639 217 211 714.6 0 211 218 205 714.6 0 205 219 306 714.6 0 306 220 182 714.6 0 182 221 113 714.6 0 113 222 136 714.6 0 136 223 1162 714.6 0 1162 224 692 714.6 0 692 225 521 714.6 0 521 226 214 714.6 0 214 227 197 714.6 0 197 228 241 714.6 0 241 229 222 714.6 0 222 230 146 714.6 0 146 231 111 714.6 0 111 232 87 714.6 0 87 233 100 714.6 0 100 234 115 714.6 0 115 235 190 714.6 0 190 236 237 714.6 0 237 237 190 714.6 0 190 238 300 714.6 0 300 239 192 714.6 0 192 240 309 714.6 0 309 241 163 714.6 0 163 242 92 714.6 0 92 243 120 714.6 0 120 244 112 714.6 0 112 245 237 714.6 0 237 246 469 714.6 0 469 247 510 714.6 0 510 248 258 714.6 0 258 249 84 714.6 0 84 250 52 714.6 0 52 251 67 714.6 0 67 252 78 714.6 0 78 253 128 714.6 0 128 254 157 714.6 0 157 255 183 714.6 0 183 256 293 714.6 0 293 257 443 714.6 0 443 258 353 714.6 0 353 259 248 714.6 0 248 260 140 714.6 0 140 261 72 714.6 0 72 262 101 714.6 0 101 263 222 714.6 0 222 264 363 714.6 0 363 265 530 714.6 0 530 266 254 714.6 0 254 267 330 714.6 0 330 268 255 714.6 0 255 269 164 714.6 0 164 270 136 714.6 0 136 271 250 714.6 0 250 272 1491 714.6 0 1491 273 3694 714.6 0 3694 274 2659 714.6 0 2659 275 154 714.6 0 154 276 106 714.6 0 106 277 122 714.6 0 122 278 218 714.6 0 218 279 256 714.6 0 256 280 212 714.6 0 212 281 161 714.6 0 161 282 58 714.6 0 58 283 132 714.6 0 132 284 213 714.6 0 213 285 569 714.6 0 569 286 589 714.6 0 589 287 384 714.6 0 384 288 132 714.6 0 132 289 80 714.6 0 80 290 52 714.6 0 52 291 89 714.6 0 89 292 136 714.6 0 136 293 249 714.6 0 249 294 193 714.6 0 193 295 150 714.6 0 150 296 128 714.6 0 128 297 120 714.6 0 120 298 212 714.6 0 212 299 599 714.6 0 599 300 834 714.6 0 834 301 633 714.6 0 633 Finished in 1.31 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 36,054 Reads with adapters: 1,876 (5.2%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 1,858 (5.2%) Total basepairs processed: 7,153,557 bp Total written (filtered): 512,076 bp (7.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1876 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 13 0.0 3 0 8 3 2 17 1806 0.0 3 1743 50 7 6 18 18 0.0 3 11 5 2 20 2 0.0 3 0 0 0 2 21 1 0.0 3 1 29 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 76 3 0.0 3 0 0 0 3 83 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 2 0.0 3 0 0 0 2 196 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 250 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.07 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 1,858 Reads with adapters: 79 (4.3%) Reads written (passing filters): 1,858 (100.0%) Total basepairs processed: 512,076 bp Total written (filtered): 511,838 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 79 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 15.2% C: 49.4% G: 16.5% T: 19.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 78 29.0 0 78 4 1 7.3 0 1