This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.20 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 37,779 (1.3%) Reads that were too short: 5,396 (0.2%) Reads written (passing filters): 32,383 (1.1%) Total basepairs processed: 881,011,047 bp Total written (filtered): 7,013,563 bp (0.8%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 37779 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 86 714.6 0 86 7 65 714.6 0 65 8 147 714.6 0 147 9 1811 714.6 0 1811 10 37 714.6 0 37 11 33 714.6 0 33 12 22 714.6 0 22 13 33 714.6 0 33 14 35 714.6 0 35 15 33 714.6 0 33 16 32 714.6 0 32 17 34 714.6 0 34 18 23 714.6 0 23 19 40 714.6 0 40 20 24 714.6 0 24 21 35 714.6 0 35 22 30 714.6 0 30 23 29 714.6 0 29 24 33 714.6 0 33 25 33 714.6 0 33 26 25 714.6 0 25 27 43 714.6 0 43 28 32 714.6 0 32 29 53 714.6 0 53 30 93 714.6 0 93 31 160 714.6 0 160 32 38 714.6 0 38 33 31 714.6 0 31 34 32 714.6 0 32 35 44 714.6 0 44 36 71 714.6 0 71 37 76 714.6 0 76 38 37 714.6 0 37 39 39 714.6 0 39 40 40 714.6 0 40 41 31 714.6 0 31 42 32 714.6 0 32 43 31 714.6 0 31 44 24 714.6 0 24 45 32 714.6 0 32 46 28 714.6 0 28 47 29 714.6 0 29 48 26 714.6 0 26 49 37 714.6 0 37 50 36 714.6 0 36 51 23 714.6 0 23 52 43 714.6 0 43 53 177 714.6 0 177 54 13541 714.6 0 13541 55 982 714.6 0 982 56 1017 714.6 0 1017 57 30 714.6 0 30 58 21 714.6 0 21 59 19 714.6 0 19 60 35 714.6 0 35 61 29 714.6 0 29 62 28 714.6 0 28 63 18 714.6 0 18 64 21 714.6 0 21 65 18 714.6 0 18 66 29 714.6 0 29 67 39 714.6 0 39 68 26 714.6 0 26 69 34 714.6 0 34 70 26 714.6 0 26 71 53 714.6 0 53 72 99 714.6 0 99 73 921 714.6 0 921 74 917 714.6 0 917 75 179 714.6 0 179 76 26 714.6 0 26 77 21 714.6 0 21 78 26 714.6 0 26 79 41 714.6 0 41 80 20 714.6 0 20 81 26 714.6 0 26 82 22 714.6 0 22 83 18 714.6 0 18 84 34 714.6 0 34 85 26 714.6 0 26 86 31 714.6 0 31 87 37 714.6 0 37 88 32 714.6 0 32 89 37 714.6 0 37 90 30 714.6 0 30 91 25 714.6 0 25 92 25 714.6 0 25 93 25 714.6 0 25 94 32 714.6 0 32 95 18 714.6 0 18 96 19 714.6 0 19 97 28 714.6 0 28 98 16 714.6 0 16 99 27 714.6 0 27 100 30 714.6 0 30 101 34 714.6 0 34 102 24 714.6 0 24 103 23 714.6 0 23 104 26 714.6 0 26 105 22 714.6 0 22 106 17 714.6 0 17 107 13 714.6 0 13 108 20 714.6 0 20 109 29 714.6 0 29 110 38 714.6 0 38 111 26 714.6 0 26 112 24 714.6 0 24 113 18 714.6 0 18 114 21 714.6 0 21 115 26 714.6 0 26 116 25 714.6 0 25 117 17 714.6 0 17 118 27 714.6 0 27 119 22 714.6 0 22 120 18 714.6 0 18 121 30 714.6 0 30 122 51 714.6 0 51 123 23 714.6 0 23 124 28 714.6 0 28 125 30 714.6 0 30 126 26 714.6 0 26 127 21 714.6 0 21 128 34 714.6 0 34 129 38 714.6 0 38 130 46 714.6 0 46 131 24 714.6 0 24 132 37 714.6 0 37 133 34 714.6 0 34 134 30 714.6 0 30 135 53 714.6 0 53 136 68 714.6 0 68 137 100 714.6 0 100 138 118 714.6 0 118 139 43 714.6 0 43 140 22 714.6 0 22 141 40 714.6 0 40 142 49 714.6 0 49 143 41 714.6 0 41 144 40 714.6 0 40 145 53 714.6 0 53 146 159 714.6 0 159 147 904 714.6 0 904 148 832 714.6 0 832 149 42 714.6 0 42 150 52 714.6 0 52 151 23 714.6 0 23 152 27 714.6 0 27 153 32 714.6 0 32 154 45 714.6 0 45 155 77 714.6 0 77 156 60 714.6 0 60 157 77 714.6 0 77 158 242 714.6 0 242 159 235 714.6 0 235 160 169 714.6 0 169 161 70 714.6 0 70 162 69 714.6 0 69 163 30 714.6 0 30 164 21 714.6 0 21 165 36 714.6 0 36 166 103 714.6 0 103 167 95 714.6 0 95 168 41 714.6 0 41 169 74 714.6 0 74 170 166 714.6 0 166 171 58 714.6 0 58 172 39 714.6 0 39 173 57 714.6 0 57 174 357 714.6 0 357 175 318 714.6 0 318 176 133 714.6 0 133 177 60 714.6 0 60 178 47 714.6 0 47 179 78 714.6 0 78 180 765 714.6 0 765 181 793 714.6 0 793 182 77 714.6 0 77 183 70 714.6 0 70 184 60 714.6 0 60 185 54 714.6 0 54 186 81 714.6 0 81 187 414 714.6 0 414 188 254 714.6 0 254 189 83 714.6 0 83 190 295 714.6 0 295 191 34 714.6 0 34 192 52 714.6 0 52 193 29 714.6 0 29 194 44 714.6 0 44 195 37 714.6 0 37 196 18 714.6 0 18 197 16 714.6 0 16 198 15 714.6 0 15 199 27 714.6 0 27 200 34 714.6 0 34 201 22 714.6 0 22 202 30 714.6 0 30 203 39 714.6 0 39 204 104 714.6 0 104 205 98 714.6 0 98 206 48 714.6 0 48 207 35 714.6 0 35 208 33 714.6 0 33 209 25 714.6 0 25 210 23 714.6 0 23 211 21 714.6 0 21 212 30 714.6 0 30 213 31 714.6 0 31 214 46 714.6 0 46 215 55 714.6 0 55 216 41 714.6 0 41 217 48 714.6 0 48 218 47 714.6 0 47 219 29 714.6 0 29 220 38 714.6 0 38 221 35 714.6 0 35 222 46 714.6 0 46 223 43 714.6 0 43 224 42 714.6 0 42 225 41 714.6 0 41 226 35 714.6 0 35 227 91 714.6 0 91 228 209 714.6 0 209 229 75 714.6 0 75 230 54 714.6 0 54 231 56 714.6 0 56 232 41 714.6 0 41 233 39 714.6 0 39 234 34 714.6 0 34 235 46 714.6 0 46 236 34 714.6 0 34 237 57 714.6 0 57 238 94 714.6 0 94 239 45 714.6 0 45 240 31 714.6 0 31 241 27 714.6 0 27 242 31 714.6 0 31 243 26 714.6 0 26 244 28 714.6 0 28 245 32 714.6 0 32 246 32 714.6 0 32 247 32 714.6 0 32 248 41 714.6 0 41 249 38 714.6 0 38 250 34 714.6 0 34 251 121 714.6 0 121 252 375 714.6 0 375 253 152 714.6 0 152 254 133 714.6 0 133 255 64 714.6 0 64 256 29 714.6 0 29 257 47 714.6 0 47 258 51 714.6 0 51 259 30 714.6 0 30 260 59 714.6 0 59 261 114 714.6 0 114 262 195 714.6 0 195 263 119 714.6 0 119 264 68 714.6 0 68 265 38 714.6 0 38 266 21 714.6 0 21 267 28 714.6 0 28 268 38 714.6 0 38 269 23 714.6 0 23 270 13 714.6 0 13 271 13 714.6 0 13 272 41 714.6 0 41 273 48 714.6 0 48 274 39 714.6 0 39 275 33 714.6 0 33 276 41 714.6 0 41 277 54 714.6 0 54 278 104 714.6 0 104 279 53 714.6 0 53 280 38 714.6 0 38 281 39 714.6 0 39 282 64 714.6 0 64 283 100 714.6 0 100 284 31 714.6 0 31 285 61 714.6 0 61 286 61 714.6 0 61 287 26 714.6 0 26 288 38 714.6 0 38 289 16 714.6 0 16 290 28 714.6 0 28 291 39 714.6 0 39 292 43 714.6 0 43 293 25 714.6 0 25 294 24 714.6 0 24 295 66 714.6 0 66 296 29 714.6 0 29 297 37 714.6 0 37 298 27 714.6 0 27 299 50 714.6 0 50 300 68 714.6 0 68 301 52 714.6 0 52 Finished in 1.41 s (44 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 32,383 Reads with adapters: 1,969 (6.1%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 1,957 (6.0%) Total basepairs processed: 7,013,563 bp Total written (filtered): 531,350 bp (7.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1969 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 18 0.0 3 0 0 0 1 17 18 3 0.0 3 0 0 0 0 3 19 32 0.0 3 0 31 0 0 1 20 1848 0.0 4 1818 28 0 0 2 21 6 0.0 4 1 4 0 0 1 22 1 0.0 4 0 1 23 37 0.0 4 35 0 0 2 39 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 81 4 0.0 4 0 0 0 0 4 107 1 0.0 4 0 0 0 0 1 111 2 0.0 4 0 0 0 1 1 125 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 1 173 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 183 2 0.0 4 0 0 0 0 2 187 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 243 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (31 us/read; 1.96 M reads/minute). === Summary === Total reads processed: 1,957 Reads with adapters: 50 (2.6%) Reads written (passing filters): 1,957 (100.0%) Total basepairs processed: 531,350 bp Total written (filtered): 530,382 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 50 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.0% C: 2.0% G: 94.0% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 2 7.6 0 2 5 1 1.9 0 1 9 2 0.0 0 1 1 10 29 0.0 1 0 29 11 14 0.0 1 0 14 223 1 0.0 1 1 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.