This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGCAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 78.18 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 303,383 (10.4%) Reads that were too short: 80,730 (2.8%) Reads written (passing filters): 222,653 (7.6%) Total basepairs processed: 881,011,047 bp Total written (filtered): 45,451,242 bp (5.2%) === Adapter 1 === Sequence: AAGCAG; Type: regular 5'; Length: 6; Trimmed: 303383 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1712 714.6 0 1712 7 60539 714.6 0 60539 8 270 714.6 0 270 9 3734 714.6 0 3734 10 103 714.6 0 103 11 111 714.6 0 111 12 85 714.6 0 85 13 84 714.6 0 84 14 59 714.6 0 59 15 80 714.6 0 80 16 75 714.6 0 75 17 107 714.6 0 107 18 85 714.6 0 85 19 79 714.6 0 79 20 83 714.6 0 83 21 125 714.6 0 125 22 141 714.6 0 141 23 170 714.6 0 170 24 217 714.6 0 217 25 366 714.6 0 366 26 259 714.6 0 259 27 84 714.6 0 84 28 88 714.6 0 88 29 94 714.6 0 94 30 79 714.6 0 79 31 78 714.6 0 78 32 93 714.6 0 93 33 102 714.6 0 102 34 58 714.6 0 58 35 84 714.6 0 84 36 89 714.6 0 89 37 66 714.6 0 66 38 93 714.6 0 93 39 102 714.6 0 102 40 84 714.6 0 84 41 82 714.6 0 82 42 64 714.6 0 64 43 82 714.6 0 82 44 86 714.6 0 86 45 92 714.6 0 92 46 79 714.6 0 79 47 83 714.6 0 83 48 82 714.6 0 82 49 89 714.6 0 89 50 97 714.6 0 97 51 96 714.6 0 96 52 73 714.6 0 73 53 86 714.6 0 86 54 81 714.6 0 81 55 138 714.6 0 138 56 86 714.6 0 86 57 74 714.6 0 74 58 81 714.6 0 81 59 76 714.6 0 76 60 97 714.6 0 97 61 259 714.6 0 259 62 438 714.6 0 438 63 417 714.6 0 417 64 84 714.6 0 84 65 64 714.6 0 64 66 77 714.6 0 77 67 75 714.6 0 75 68 87 714.6 0 87 69 135 714.6 0 135 70 295 714.6 0 295 71 896 714.6 0 896 72 299 714.6 0 299 73 515 714.6 0 515 74 409 714.6 0 409 75 213 714.6 0 213 76 467 714.6 0 467 77 772 714.6 0 772 78 1000 714.6 0 1000 79 664 714.6 0 664 80 1317 714.6 0 1317 81 1003 714.6 0 1003 82 1877 714.6 0 1877 83 3565 714.6 0 3565 84 1912 714.6 0 1912 85 1216 714.6 0 1216 86 1199 714.6 0 1199 87 564 714.6 0 564 88 464 714.6 0 464 89 1099 714.6 0 1099 90 524 714.6 0 524 91 669 714.6 0 669 92 1306 714.6 0 1306 93 800 714.6 0 800 94 606 714.6 0 606 95 791 714.6 0 791 96 471 714.6 0 471 97 442 714.6 0 442 98 419 714.6 0 419 99 540 714.6 0 540 100 548 714.6 0 548 101 569 714.6 0 569 102 485 714.6 0 485 103 325 714.6 0 325 104 263 714.6 0 263 105 349 714.6 0 349 106 313 714.6 0 313 107 346 714.6 0 346 108 500 714.6 0 500 109 685 714.6 0 685 110 918 714.6 0 918 111 884 714.6 0 884 112 4227 714.6 0 4227 113 5901 714.6 0 5901 114 8860 714.6 0 8860 115 654 714.6 0 654 116 736 714.6 0 736 117 890 714.6 0 890 118 1179 714.6 0 1179 119 1318 714.6 0 1318 120 1419 714.6 0 1419 121 1805 714.6 0 1805 122 1926 714.6 0 1926 123 708 714.6 0 708 124 765 714.6 0 765 125 973 714.6 0 973 126 836 714.6 0 836 127 1131 714.6 0 1131 128 758 714.6 0 758 129 653 714.6 0 653 130 743 714.6 0 743 131 769 714.6 0 769 132 859 714.6 0 859 133 709 714.6 0 709 134 793 714.6 0 793 135 825 714.6 0 825 136 1094 714.6 0 1094 137 772 714.6 0 772 138 790 714.6 0 790 139 963 714.6 0 963 140 833 714.6 0 833 141 867 714.6 0 867 142 1266 714.6 0 1266 143 1434 714.6 0 1434 144 1252 714.6 0 1252 145 947 714.6 0 947 146 906 714.6 0 906 147 935 714.6 0 935 148 1466 714.6 0 1466 149 1287 714.6 0 1287 150 865 714.6 0 865 151 1328 714.6 0 1328 152 1078 714.6 0 1078 153 1012 714.6 0 1012 154 1111 714.6 0 1111 155 848 714.6 0 848 156 718 714.6 0 718 157 836 714.6 0 836 158 798 714.6 0 798 159 745 714.6 0 745 160 748 714.6 0 748 161 834 714.6 0 834 162 780 714.6 0 780 163 913 714.6 0 913 164 974 714.6 0 974 165 910 714.6 0 910 166 1408 714.6 0 1408 167 1243 714.6 0 1243 168 1318 714.6 0 1318 169 1350 714.6 0 1350 170 1137 714.6 0 1137 171 1893 714.6 0 1893 172 2545 714.6 0 2545 173 1859 714.6 0 1859 174 1340 714.6 0 1340 175 1263 714.6 0 1263 176 902 714.6 0 902 177 994 714.6 0 994 178 1041 714.6 0 1041 179 925 714.6 0 925 180 1013 714.6 0 1013 181 958 714.6 0 958 182 920 714.6 0 920 183 1968 714.6 0 1968 184 2232 714.6 0 2232 185 1422 714.6 0 1422 186 932 714.6 0 932 187 919 714.6 0 919 188 968 714.6 0 968 189 918 714.6 0 918 190 1156 714.6 0 1156 191 2606 714.6 0 2606 192 2372 714.6 0 2372 193 1963 714.6 0 1963 194 1461 714.6 0 1461 195 930 714.6 0 930 196 722 714.6 0 722 197 1213 714.6 0 1213 198 1213 714.6 0 1213 199 1678 714.6 0 1678 200 919 714.6 0 919 201 668 714.6 0 668 202 730 714.6 0 730 203 964 714.6 0 964 204 861 714.6 0 861 205 837 714.6 0 837 206 766 714.6 0 766 207 736 714.6 0 736 208 689 714.6 0 689 209 728 714.6 0 728 210 732 714.6 0 732 211 877 714.6 0 877 212 729 714.6 0 729 213 809 714.6 0 809 214 730 714.6 0 730 215 893 714.6 0 893 216 878 714.6 0 878 217 815 714.6 0 815 218 967 714.6 0 967 219 989 714.6 0 989 220 970 714.6 0 970 221 516 714.6 0 516 222 530 714.6 0 530 223 956 714.6 0 956 224 851 714.6 0 851 225 856 714.6 0 856 226 727 714.6 0 727 227 798 714.6 0 798 228 918 714.6 0 918 229 909 714.6 0 909 230 752 714.6 0 752 231 618 714.6 0 618 232 733 714.6 0 733 233 733 714.6 0 733 234 812 714.6 0 812 235 900 714.6 0 900 236 868 714.6 0 868 237 505 714.6 0 505 238 586 714.6 0 586 239 965 714.6 0 965 240 706 714.6 0 706 241 558 714.6 0 558 242 660 714.6 0 660 243 927 714.6 0 927 244 786 714.6 0 786 245 821 714.6 0 821 246 868 714.6 0 868 247 833 714.6 0 833 248 445 714.6 0 445 249 490 714.6 0 490 250 458 714.6 0 458 251 893 714.6 0 893 252 2289 714.6 0 2289 253 1286 714.6 0 1286 254 759 714.6 0 759 255 715 714.6 0 715 256 763 714.6 0 763 257 711 714.6 0 711 258 485 714.6 0 485 259 363 714.6 0 363 260 365 714.6 0 365 261 543 714.6 0 543 262 984 714.6 0 984 263 1016 714.6 0 1016 264 919 714.6 0 919 265 1029 714.6 0 1029 266 703 714.6 0 703 267 921 714.6 0 921 268 1461 714.6 0 1461 269 922 714.6 0 922 270 671 714.6 0 671 271 756 714.6 0 756 272 785 714.6 0 785 273 646 714.6 0 646 274 609 714.6 0 609 275 529 714.6 0 529 276 361 714.6 0 361 277 596 714.6 0 596 278 1161 714.6 0 1161 279 974 714.6 0 974 280 778 714.6 0 778 281 603 714.6 0 603 282 590 714.6 0 590 283 1073 714.6 0 1073 284 1001 714.6 0 1001 285 722 714.6 0 722 286 818 714.6 0 818 287 684 714.6 0 684 288 468 714.6 0 468 289 747 714.6 0 747 290 982 714.6 0 982 291 1005 714.6 0 1005 292 1152 714.6 0 1152 293 1169 714.6 0 1169 294 683 714.6 0 683 295 675 714.6 0 675 296 732 714.6 0 732 297 955 714.6 0 955 298 2184 714.6 0 2184 299 784 714.6 0 784 300 764 714.6 0 764 301 161 714.6 0 161 Finished in 7.98 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 222,653 Reads with adapters: 4,312 (1.9%) Reads that were too short: 304 (0.1%) Reads written (passing filters): 4,008 (1.8%) Total basepairs processed: 45,451,242 bp Total written (filtered): 1,066,092 bp (2.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4312 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 0 1 16 31 0.0 3 0 24 1 6 17 3701 0.0 3 3579 101 15 6 18 29 0.0 3 6 16 2 5 19 4 0.0 3 0 0 1 3 20 4 0.0 3 0 0 0 4 21 1 0.0 3 0 0 0 1 23 5 0.0 3 0 0 1 4 24 66 0.0 3 0 0 1 65 26 5 0.0 3 0 0 0 5 27 8 0.0 3 0 0 0 8 28 5 0.0 3 0 0 0 5 29 1 0.0 3 0 0 0 1 30 2 0.0 3 0 0 0 2 32 2 0.0 3 0 0 0 2 33 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 2 0.0 3 0 0 0 2 39 2 0.0 3 0 0 0 2 41 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 51 13 0.0 3 0 0 0 13 53 2 0.0 3 0 0 0 2 54 4 0.0 3 0 0 0 4 57 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 59 4 0.0 3 0 0 0 4 60 3 0.0 3 0 0 0 3 61 1 0.0 3 0 0 0 1 62 3 0.0 3 0 0 0 3 64 1 0.0 3 0 0 0 1 66 18 0.0 3 0 0 0 18 67 1 0.0 3 0 0 0 1 68 4 0.0 3 0 0 0 4 69 2 0.0 3 0 0 0 2 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 3 0.0 3 0 0 0 3 77 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 79 11 0.0 3 0 0 0 11 81 1 0.0 3 0 0 0 1 82 7 0.0 3 0 0 0 7 83 2 0.0 3 0 0 0 2 84 33 0.0 3 0 0 0 33 85 1 0.0 3 0 0 0 1 87 3 0.0 3 0 0 0 3 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 15 0.0 3 0 0 1 14 104 2 0.0 3 0 0 0 2 105 6 0.0 3 0 0 0 6 107 3 0.0 3 0 0 0 3 108 4 0.0 3 0 0 0 4 109 4 0.0 3 0 0 0 4 110 6 0.0 3 0 0 0 6 111 5 0.0 3 0 0 0 5 112 11 0.0 3 0 0 0 11 113 6 0.0 3 0 0 0 6 115 9 0.0 3 0 0 1 8 116 2 0.0 3 0 0 0 2 118 4 0.0 3 0 0 0 4 119 1 0.0 3 0 0 0 1 121 3 0.0 3 0 0 0 3 122 4 0.0 3 0 0 0 4 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 0 2 133 3 0.0 3 0 0 0 3 134 17 0.0 3 0 0 0 17 136 12 0.0 3 0 0 1 11 138 2 0.0 3 0 0 0 2 139 8 0.0 3 0 0 0 8 140 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 3 0.0 3 0 0 0 3 149 2 0.0 3 0 0 1 1 150 4 0.0 3 0 0 0 4 152 2 0.0 3 0 0 0 2 153 3 0.0 3 0 0 0 3 154 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 162 3 0.0 3 0 0 0 3 163 2 0.0 3 0 0 0 2 165 3 0.0 3 0 0 0 3 167 6 0.0 3 0 0 0 6 168 3 0.0 3 0 0 0 3 169 3 0.0 3 0 0 0 3 171 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 181 43 0.0 3 0 0 0 43 185 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 2 0.0 3 0 0 0 2 210 2 0.0 3 0 1 0 1 217 1 0.0 3 0 0 0 1 218 6 0.0 3 0 0 0 6 222 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 252 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 270 3 0.0 3 0 0 0 3 280 1 0.0 3 0 0 0 1 281 8 0.0 3 0 0 0 8 282 20 0.0 3 0 0 0 20 283 7 0.0 3 0 0 0 7 288 2 0.0 3 0 0 0 2 289 3 0.0 3 0 0 0 3 291 2 0.0 3 0 0 0 2 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (37 us/read; 1.60 M reads/minute). === Summary === Total reads processed: 4,008 Reads with adapters: 219 (5.5%) Reads written (passing filters): 4,008 (100.0%) Total basepairs processed: 1,066,092 bp Total written (filtered): 1,065,432 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 219 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 53.0% C: 10.0% G: 30.6% T: 6.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 216 62.6 0 216 4 3 15.7 0 3