This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 78.38 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 172,254 (5.9%) Reads that were too short: 86,375 (3.0%) Reads written (passing filters): 85,879 (2.9%) Total basepairs processed: 881,011,047 bp Total written (filtered): 14,070,943 bp (1.6%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 172254 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 25 714.6 0 25 7 278 714.6 0 278 8 3702 714.6 0 3702 9 10 714.6 0 10 10 16 714.6 0 16 11 17 714.6 0 17 12 17 714.6 0 17 13 58 714.6 0 58 14 47 714.6 0 47 15 37 714.6 0 37 16 17 714.6 0 17 17 17 714.6 0 17 18 12 714.6 0 12 19 12 714.6 0 12 20 9 714.6 0 9 21 12 714.6 0 12 22 15 714.6 0 15 23 11 714.6 0 11 24 27 714.6 0 27 25 2045 714.6 0 2045 26 143 714.6 0 143 27 15 714.6 0 15 28 10 714.6 0 10 29 11 714.6 0 11 30 7 714.6 0 7 31 7 714.6 0 7 32 17 714.6 0 17 33 16 714.6 0 16 34 14 714.6 0 14 35 5 714.6 0 5 36 17 714.6 0 17 37 14 714.6 0 14 38 8 714.6 0 8 39 12 714.6 0 12 40 7 714.6 0 7 41 15 714.6 0 15 42 7 714.6 0 7 43 16 714.6 0 16 44 10 714.6 0 10 45 23 714.6 0 23 46 9 714.6 0 9 47 10 714.6 0 10 48 12 714.6 0 12 49 9 714.6 0 9 50 16 714.6 0 16 51 11 714.6 0 11 52 12 714.6 0 12 53 10 714.6 0 10 54 14 714.6 0 14 55 18 714.6 0 18 56 23 714.6 0 23 57 13 714.6 0 13 58 8 714.6 0 8 59 19 714.6 0 19 60 13 714.6 0 13 61 17 714.6 0 17 62 142 714.6 0 142 63 20 714.6 0 20 64 7 714.6 0 7 65 12 714.6 0 12 66 17 714.6 0 17 67 14 714.6 0 14 68 11 714.6 0 11 69 13 714.6 0 13 70 14 714.6 0 14 71 13 714.6 0 13 72 8 714.6 0 8 73 15 714.6 0 15 74 20 714.6 0 20 75 29 714.6 0 29 76 12 714.6 0 12 77 22 714.6 0 22 78 28 714.6 0 28 79 61 714.6 0 61 80 122 714.6 0 122 81 263 714.6 0 263 82 971 714.6 0 971 83 969 714.6 0 969 84 392 714.6 0 392 85 309 714.6 0 309 86 167 714.6 0 167 87 218 714.6 0 218 88 908 714.6 0 908 89 624 714.6 0 624 90 431 714.6 0 431 91 228 714.6 0 228 92 143 714.6 0 143 93 261 714.6 0 261 94 458 714.6 0 458 95 286 714.6 0 286 96 332 714.6 0 332 97 227 714.6 0 227 98 514 714.6 0 514 99 629 714.6 0 629 100 295 714.6 0 295 101 198 714.6 0 198 102 569 714.6 0 569 103 1083 714.6 0 1083 104 464 714.6 0 464 105 293 714.6 0 293 106 346 714.6 0 346 107 396 714.6 0 396 108 103 714.6 0 103 109 116 714.6 0 116 110 278 714.6 0 278 111 710 714.6 0 710 112 846 714.6 0 846 113 451 714.6 0 451 114 590 714.6 0 590 115 325 714.6 0 325 116 235 714.6 0 235 117 243 714.6 0 243 118 575 714.6 0 575 119 822 714.6 0 822 120 909 714.6 0 909 121 510 714.6 0 510 122 499 714.6 0 499 123 690 714.6 0 690 124 704 714.6 0 704 125 441 714.6 0 441 126 427 714.6 0 427 127 605 714.6 0 605 128 469 714.6 0 469 129 524 714.6 0 524 130 562 714.6 0 562 131 470 714.6 0 470 132 505 714.6 0 505 133 537 714.6 0 537 134 822 714.6 0 822 135 511 714.6 0 511 136 446 714.6 0 446 137 502 714.6 0 502 138 614 714.6 0 614 139 1207 714.6 0 1207 140 1522 714.6 0 1522 141 959 714.6 0 959 142 526 714.6 0 526 143 481 714.6 0 481 144 741 714.6 0 741 145 678 714.6 0 678 146 618 714.6 0 618 147 2538 714.6 0 2538 148 672 714.6 0 672 149 489 714.6 0 489 150 484 714.6 0 484 151 525 714.6 0 525 152 633 714.6 0 633 153 716 714.6 0 716 154 570 714.6 0 570 155 563 714.6 0 563 156 491 714.6 0 491 157 632 714.6 0 632 158 701 714.6 0 701 159 859 714.6 0 859 160 1911 714.6 0 1911 161 2006 714.6 0 2006 162 1165 714.6 0 1165 163 587 714.6 0 587 164 660 714.6 0 660 165 558 714.6 0 558 166 412 714.6 0 412 167 461 714.6 0 461 168 750 714.6 0 750 169 730 714.6 0 730 170 483 714.6 0 483 171 435 714.6 0 435 172 545 714.6 0 545 173 815 714.6 0 815 174 582 714.6 0 582 175 526 714.6 0 526 176 602 714.6 0 602 177 723 714.6 0 723 178 595 714.6 0 595 179 704 714.6 0 704 180 874 714.6 0 874 181 794 714.6 0 794 182 1005 714.6 0 1005 183 673 714.6 0 673 184 663 714.6 0 663 185 801 714.6 0 801 186 856 714.6 0 856 187 2013 714.6 0 2013 188 1509 714.6 0 1509 189 1393 714.6 0 1393 190 762 714.6 0 762 191 573 714.6 0 573 192 532 714.6 0 532 193 585 714.6 0 585 194 476 714.6 0 476 195 536 714.6 0 536 196 773 714.6 0 773 197 876 714.6 0 876 198 533 714.6 0 533 199 480 714.6 0 480 200 622 714.6 0 622 201 1128 714.6 0 1128 202 1401 714.6 0 1401 203 687 714.6 0 687 204 635 714.6 0 635 205 652 714.6 0 652 206 999 714.6 0 999 207 1008 714.6 0 1008 208 915 714.6 0 915 209 732 714.6 0 732 210 829 714.6 0 829 211 790 714.6 0 790 212 763 714.6 0 763 213 657 714.6 0 657 214 818 714.6 0 818 215 665 714.6 0 665 216 718 714.6 0 718 217 630 714.6 0 630 218 759 714.6 0 759 219 879 714.6 0 879 220 908 714.6 0 908 221 1008 714.6 0 1008 222 994 714.6 0 994 223 747 714.6 0 747 224 684 714.6 0 684 225 712 714.6 0 712 226 993 714.6 0 993 227 820 714.6 0 820 228 683 714.6 0 683 229 932 714.6 0 932 230 1077 714.6 0 1077 231 1024 714.6 0 1024 232 838 714.6 0 838 233 829 714.6 0 829 234 847 714.6 0 847 235 661 714.6 0 661 236 495 714.6 0 495 237 618 714.6 0 618 238 823 714.6 0 823 239 1026 714.6 0 1026 240 1138 714.6 0 1138 241 1211 714.6 0 1211 242 1103 714.6 0 1103 243 884 714.6 0 884 244 685 714.6 0 685 245 823 714.6 0 823 246 857 714.6 0 857 247 492 714.6 0 492 248 372 714.6 0 372 249 369 714.6 0 369 250 983 714.6 0 983 251 1219 714.6 0 1219 252 816 714.6 0 816 253 539 714.6 0 539 254 705 714.6 0 705 255 729 714.6 0 729 256 891 714.6 0 891 257 756 714.6 0 756 258 718 714.6 0 718 259 625 714.6 0 625 260 711 714.6 0 711 261 679 714.6 0 679 262 781 714.6 0 781 263 690 714.6 0 690 264 890 714.6 0 890 265 722 714.6 0 722 266 921 714.6 0 921 267 586 714.6 0 586 268 465 714.6 0 465 269 630 714.6 0 630 270 1119 714.6 0 1119 271 1019 714.6 0 1019 272 681 714.6 0 681 273 683 714.6 0 683 274 963 714.6 0 963 275 1291 714.6 0 1291 276 918 714.6 0 918 277 1031 714.6 0 1031 278 1230 714.6 0 1230 279 1078 714.6 0 1078 280 573 714.6 0 573 281 689 714.6 0 689 282 1055 714.6 0 1055 283 1264 714.6 0 1264 284 741 714.6 0 741 285 906 714.6 0 906 286 743 714.6 0 743 287 563 714.6 0 563 288 504 714.6 0 504 289 802 714.6 0 802 290 1042 714.6 0 1042 291 883 714.6 0 883 292 919 714.6 0 919 293 708 714.6 0 708 294 672 714.6 0 672 295 1367 714.6 0 1367 296 779 714.6 0 779 297 716 714.6 0 716 298 1023 714.6 0 1023 299 1146 714.6 0 1146 300 1354 714.6 0 1354 301 3667 714.6 0 3667 Finished in 3.07 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 85,879 Reads with adapters: 3,932 (4.6%) Reads that were too short: 37 (0.0%) Reads written (passing filters): 3,895 (4.5%) Total basepairs processed: 14,070,943 bp Total written (filtered): 1,061,783 bp (7.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 3932 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 1 2 18 3 0.0 3 0 0 1 0 2 19 38 0.0 3 0 34 1 1 2 20 3824 0.0 4 3784 39 1 21 11 0.0 4 2 9 22 3 0.0 4 0 0 0 0 3 23 2 0.0 4 0 0 0 1 1 24 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 58 2 0.0 4 0 0 0 0 2 60 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 1 69 1 0.0 4 0 0 0 0 1 70 3 0.0 4 0 0 0 0 3 72 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 1 125 1 0.0 4 0 0 0 0 1 127 3 0.0 4 0 0 0 0 3 133 2 0.0 4 0 0 0 0 2 136 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 181 2 0.0 4 0 0 0 0 2 205 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 261 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.14 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 3,895 Reads with adapters: 240 (6.2%) Reads written (passing filters): 3,895 (100.0%) Total basepairs processed: 1,061,783 bp Total written (filtered): 1,059,161 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 240 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 99.2% T: 0.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 7 1 0.2 0 1 10 14 0.0 1 0 14 11 225 0.0 1 1 224 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.