This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGAGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 72.84 s (25 us/read; 2.41 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 54,528 (1.9%) Reads that were too short: 20,076 (0.7%) Reads written (passing filters): 34,452 (1.2%) Total basepairs processed: 881,011,047 bp Total written (filtered): 7,784,305 bp (0.9%) === Adapter 1 === Sequence: TTGAGT; Type: regular 5'; Length: 6; Trimmed: 54528 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 156 714.6 0 156 7 271 714.6 0 271 8 10420 714.6 0 10420 9 2601 714.6 0 2601 10 76 714.6 0 76 11 54 714.6 0 54 12 65 714.6 0 65 13 67 714.6 0 67 14 59 714.6 0 59 15 53 714.6 0 53 16 73 714.6 0 73 17 57 714.6 0 57 18 114 714.6 0 114 19 75 714.6 0 75 20 68 714.6 0 68 21 81 714.6 0 81 22 53 714.6 0 53 23 57 714.6 0 57 24 54 714.6 0 54 25 55 714.6 0 55 26 72 714.6 0 72 27 59 714.6 0 59 28 57 714.6 0 57 29 86 714.6 0 86 30 96 714.6 0 96 31 61 714.6 0 61 32 73 714.6 0 73 33 61 714.6 0 61 34 58 714.6 0 58 35 52 714.6 0 52 36 88 714.6 0 88 37 78 714.6 0 78 38 61 714.6 0 61 39 47 714.6 0 47 40 55 714.6 0 55 41 67 714.6 0 67 42 59 714.6 0 59 43 59 714.6 0 59 44 68 714.6 0 68 45 56 714.6 0 56 46 51 714.6 0 51 47 81 714.6 0 81 48 63 714.6 0 63 49 54 714.6 0 54 50 56 714.6 0 56 51 63 714.6 0 63 52 68 714.6 0 68 53 55 714.6 0 55 54 76 714.6 0 76 55 43 714.6 0 43 56 55 714.6 0 55 57 59 714.6 0 59 58 55 714.6 0 55 59 275 714.6 0 275 60 224 714.6 0 224 61 87 714.6 0 87 62 70 714.6 0 70 63 79 714.6 0 79 64 61 714.6 0 61 65 51 714.6 0 51 66 70 714.6 0 70 67 57 714.6 0 57 68 57 714.6 0 57 69 66 714.6 0 66 70 72 714.6 0 72 71 67 714.6 0 67 72 130 714.6 0 130 73 88 714.6 0 88 74 66 714.6 0 66 75 73 714.6 0 73 76 185 714.6 0 185 77 198 714.6 0 198 78 325 714.6 0 325 79 91 714.6 0 91 80 96 714.6 0 96 81 125 714.6 0 125 82 119 714.6 0 119 83 97 714.6 0 97 84 136 714.6 0 136 85 147 714.6 0 147 86 126 714.6 0 126 87 101 714.6 0 101 88 98 714.6 0 98 89 60 714.6 0 60 90 83 714.6 0 83 91 97 714.6 0 97 92 151 714.6 0 151 93 195 714.6 0 195 94 188 714.6 0 188 95 117 714.6 0 117 96 119 714.6 0 119 97 120 714.6 0 120 98 273 714.6 0 273 99 122 714.6 0 122 100 132 714.6 0 132 101 88 714.6 0 88 102 79 714.6 0 79 103 60 714.6 0 60 104 71 714.6 0 71 105 56 714.6 0 56 106 82 714.6 0 82 107 59 714.6 0 59 108 68 714.6 0 68 109 101 714.6 0 101 110 125 714.6 0 125 111 91 714.6 0 91 112 162 714.6 0 162 113 132 714.6 0 132 114 234 714.6 0 234 115 102 714.6 0 102 116 103 714.6 0 103 117 124 714.6 0 124 118 121 714.6 0 121 119 113 714.6 0 113 120 82 714.6 0 82 121 95 714.6 0 95 122 71 714.6 0 71 123 93 714.6 0 93 124 101 714.6 0 101 125 106 714.6 0 106 126 100 714.6 0 100 127 105 714.6 0 105 128 131 714.6 0 131 129 176 714.6 0 176 130 198 714.6 0 198 131 165 714.6 0 165 132 258 714.6 0 258 133 169 714.6 0 169 134 161 714.6 0 161 135 114 714.6 0 114 136 117 714.6 0 117 137 103 714.6 0 103 138 230 714.6 0 230 139 196 714.6 0 196 140 143 714.6 0 143 141 106 714.6 0 106 142 132 714.6 0 132 143 144 714.6 0 144 144 160 714.6 0 160 145 142 714.6 0 142 146 111 714.6 0 111 147 143 714.6 0 143 148 127 714.6 0 127 149 82 714.6 0 82 150 101 714.6 0 101 151 116 714.6 0 116 152 138 714.6 0 138 153 142 714.6 0 142 154 100 714.6 0 100 155 104 714.6 0 104 156 125 714.6 0 125 157 137 714.6 0 137 158 110 714.6 0 110 159 196 714.6 0 196 160 141 714.6 0 141 161 102 714.6 0 102 162 106 714.6 0 106 163 105 714.6 0 105 164 129 714.6 0 129 165 126 714.6 0 126 166 126 714.6 0 126 167 112 714.6 0 112 168 118 714.6 0 118 169 100 714.6 0 100 170 100 714.6 0 100 171 120 714.6 0 120 172 114 714.6 0 114 173 97 714.6 0 97 174 96 714.6 0 96 175 90 714.6 0 90 176 107 714.6 0 107 177 112 714.6 0 112 178 119 714.6 0 119 179 110 714.6 0 110 180 148 714.6 0 148 181 134 714.6 0 134 182 133 714.6 0 133 183 95 714.6 0 95 184 135 714.6 0 135 185 146 714.6 0 146 186 117 714.6 0 117 187 108 714.6 0 108 188 125 714.6 0 125 189 100 714.6 0 100 190 100 714.6 0 100 191 126 714.6 0 126 192 146 714.6 0 146 193 142 714.6 0 142 194 91 714.6 0 91 195 150 714.6 0 150 196 112 714.6 0 112 197 262 714.6 0 262 198 429 714.6 0 429 199 220 714.6 0 220 200 110 714.6 0 110 201 97 714.6 0 97 202 96 714.6 0 96 203 76 714.6 0 76 204 96 714.6 0 96 205 149 714.6 0 149 206 124 714.6 0 124 207 138 714.6 0 138 208 110 714.6 0 110 209 106 714.6 0 106 210 124 714.6 0 124 211 180 714.6 0 180 212 164 714.6 0 164 213 138 714.6 0 138 214 138 714.6 0 138 215 128 714.6 0 128 216 117 714.6 0 117 217 143 714.6 0 143 218 155 714.6 0 155 219 135 714.6 0 135 220 126 714.6 0 126 221 134 714.6 0 134 222 77 714.6 0 77 223 195 714.6 0 195 224 237 714.6 0 237 225 174 714.6 0 174 226 115 714.6 0 115 227 121 714.6 0 121 228 90 714.6 0 90 229 128 714.6 0 128 230 124 714.6 0 124 231 204 714.6 0 204 232 161 714.6 0 161 233 225 714.6 0 225 234 145 714.6 0 145 235 124 714.6 0 124 236 158 714.6 0 158 237 214 714.6 0 214 238 162 714.6 0 162 239 155 714.6 0 155 240 192 714.6 0 192 241 143 714.6 0 143 242 135 714.6 0 135 243 102 714.6 0 102 244 103 714.6 0 103 245 184 714.6 0 184 246 168 714.6 0 168 247 184 714.6 0 184 248 99 714.6 0 99 249 130 714.6 0 130 250 98 714.6 0 98 251 62 714.6 0 62 252 79 714.6 0 79 253 114 714.6 0 114 254 159 714.6 0 159 255 111 714.6 0 111 256 157 714.6 0 157 257 113 714.6 0 113 258 141 714.6 0 141 259 113 714.6 0 113 260 96 714.6 0 96 261 86 714.6 0 86 262 90 714.6 0 90 263 90 714.6 0 90 264 120 714.6 0 120 265 196 714.6 0 196 266 212 714.6 0 212 267 269 714.6 0 269 268 281 714.6 0 281 269 103 714.6 0 103 270 89 714.6 0 89 271 54 714.6 0 54 272 132 714.6 0 132 273 111 714.6 0 111 274 109 714.6 0 109 275 115 714.6 0 115 276 90 714.6 0 90 277 103 714.6 0 103 278 79 714.6 0 79 279 107 714.6 0 107 280 116 714.6 0 116 281 161 714.6 0 161 282 131 714.6 0 131 283 105 714.6 0 105 284 148 714.6 0 148 285 150 714.6 0 150 286 181 714.6 0 181 287 161 714.6 0 161 288 219 714.6 0 219 289 191 714.6 0 191 290 223 714.6 0 223 291 222 714.6 0 222 292 228 714.6 0 228 293 230 714.6 0 230 294 250 714.6 0 250 295 247 714.6 0 247 296 230 714.6 0 230 297 245 714.6 0 245 298 674 714.6 0 674 299 1874 714.6 0 1874 300 2761 714.6 0 2761 301 824 714.6 0 824 Finished in 1.45 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 34,452 Reads with adapters: 10,477 (30.4%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 10,468 (30.4%) Total basepairs processed: 7,784,305 bp Total written (filtered): 2,887,050 bp (37.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10477 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 1 0.0 3 0 0 0 1 16 83 0.0 3 0 75 3 5 17 10291 0.0 3 9929 305 48 9 18 34 0.0 3 1 24 3 6 19 13 0.0 3 0 0 7 6 20 27 0.0 3 0 0 1 26 26 1 0.0 3 1 28 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 189 2 0.0 3 0 0 0 2 276 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.37 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 10,468 Reads with adapters: 107 (1.0%) Reads written (passing filters): 10,468 (100.0%) Total basepairs processed: 2,887,050 bp Total written (filtered): 2,886,727 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 107 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 54.2% C: 13.1% G: 27.1% T: 5.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 105 163.6 0 105 4 2 40.9 0 2