This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.36 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 125,567 (4.3%) Reads that were too short: 54,171 (1.9%) Reads written (passing filters): 71,396 (2.4%) Total basepairs processed: 881,011,047 bp Total written (filtered): 12,740,610 bp (1.4%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 125567 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1193 714.6 0 1193 7 9591 714.6 0 9591 8 3436 714.6 0 3436 9 43 714.6 0 43 10 21 714.6 0 21 11 16 714.6 0 16 12 21 714.6 0 21 13 15 714.6 0 15 14 21 714.6 0 21 15 13 714.6 0 13 16 16 714.6 0 16 17 9 714.6 0 9 18 20 714.6 0 20 19 22 714.6 0 22 20 23 714.6 0 23 21 18 714.6 0 18 22 34 714.6 0 34 23 13 714.6 0 13 24 12 714.6 0 12 25 20 714.6 0 20 26 18 714.6 0 18 27 21 714.6 0 21 28 20 714.6 0 20 29 18 714.6 0 18 30 20 714.6 0 20 31 8 714.6 0 8 32 18 714.6 0 18 33 8 714.6 0 8 34 48 714.6 0 48 35 25 714.6 0 25 36 21 714.6 0 21 37 15 714.6 0 15 38 33 714.6 0 33 39 23 714.6 0 23 40 17 714.6 0 17 41 14 714.6 0 14 42 22 714.6 0 22 43 31 714.6 0 31 44 6 714.6 0 6 45 16 714.6 0 16 46 20 714.6 0 20 47 14 714.6 0 14 48 18 714.6 0 18 49 12 714.6 0 12 50 18 714.6 0 18 51 9 714.6 0 9 52 9 714.6 0 9 53 66 714.6 0 66 54 44 714.6 0 44 55 19 714.6 0 19 56 18 714.6 0 18 57 19 714.6 0 19 58 9 714.6 0 9 59 12 714.6 0 12 60 25 714.6 0 25 61 13 714.6 0 13 62 8 714.6 0 8 63 16 714.6 0 16 64 16 714.6 0 16 65 11 714.6 0 11 66 16 714.6 0 16 67 20 714.6 0 20 68 15 714.6 0 15 69 16 714.6 0 16 70 24 714.6 0 24 71 10 714.6 0 10 72 10 714.6 0 10 73 7 714.6 0 7 74 10 714.6 0 10 75 7 714.6 0 7 76 8 714.6 0 8 77 10 714.6 0 10 78 13 714.6 0 13 79 16 714.6 0 16 80 19 714.6 0 19 81 47 714.6 0 47 82 53 714.6 0 53 83 74 714.6 0 74 84 55 714.6 0 55 85 53 714.6 0 53 86 51 714.6 0 51 87 77 714.6 0 77 88 74 714.6 0 74 89 78 714.6 0 78 90 54 714.6 0 54 91 138 714.6 0 138 92 76 714.6 0 76 93 262 714.6 0 262 94 231 714.6 0 231 95 132 714.6 0 132 96 63 714.6 0 63 97 75 714.6 0 75 98 108 714.6 0 108 99 76 714.6 0 76 100 39 714.6 0 39 101 136 714.6 0 136 102 138 714.6 0 138 103 249 714.6 0 249 104 210 714.6 0 210 105 208 714.6 0 208 106 99 714.6 0 99 107 298 714.6 0 298 108 93 714.6 0 93 109 115 714.6 0 115 110 82 714.6 0 82 111 470 714.6 0 470 112 408 714.6 0 408 113 530 714.6 0 530 114 432 714.6 0 432 115 278 714.6 0 278 116 109 714.6 0 109 117 93 714.6 0 93 118 104 714.6 0 104 119 101 714.6 0 101 120 152 714.6 0 152 121 123 714.6 0 123 122 136 714.6 0 136 123 177 714.6 0 177 124 217 714.6 0 217 125 1094 714.6 0 1094 126 2156 714.6 0 2156 127 804 714.6 0 804 128 196 714.6 0 196 129 200 714.6 0 200 130 367 714.6 0 367 131 172 714.6 0 172 132 334 714.6 0 334 133 268 714.6 0 268 134 369 714.6 0 369 135 197 714.6 0 197 136 171 714.6 0 171 137 205 714.6 0 205 138 288 714.6 0 288 139 329 714.6 0 329 140 306 714.6 0 306 141 238 714.6 0 238 142 197 714.6 0 197 143 236 714.6 0 236 144 678 714.6 0 678 145 623 714.6 0 623 146 580 714.6 0 580 147 363 714.6 0 363 148 226 714.6 0 226 149 290 714.6 0 290 150 684 714.6 0 684 151 1266 714.6 0 1266 152 681 714.6 0 681 153 347 714.6 0 347 154 426 714.6 0 426 155 1565 714.6 0 1565 156 639 714.6 0 639 157 776 714.6 0 776 158 714 714.6 0 714 159 926 714.6 0 926 160 6365 714.6 0 6365 161 4919 714.6 0 4919 162 3043 714.6 0 3043 163 411 714.6 0 411 164 716 714.6 0 716 165 658 714.6 0 658 166 422 714.6 0 422 167 273 714.6 0 273 168 286 714.6 0 286 169 337 714.6 0 337 170 308 714.6 0 308 171 285 714.6 0 285 172 241 714.6 0 241 173 277 714.6 0 277 174 265 714.6 0 265 175 298 714.6 0 298 176 296 714.6 0 296 177 416 714.6 0 416 178 376 714.6 0 376 179 441 714.6 0 441 180 1195 714.6 0 1195 181 1210 714.6 0 1210 182 1126 714.6 0 1126 183 409 714.6 0 409 184 271 714.6 0 271 185 333 714.6 0 333 186 688 714.6 0 688 187 365 714.6 0 365 188 297 714.6 0 297 189 413 714.6 0 413 190 475 714.6 0 475 191 399 714.6 0 399 192 215 714.6 0 215 193 143 714.6 0 143 194 115 714.6 0 115 195 197 714.6 0 197 196 236 714.6 0 236 197 254 714.6 0 254 198 233 714.6 0 233 199 266 714.6 0 266 200 281 714.6 0 281 201 421 714.6 0 421 202 247 714.6 0 247 203 139 714.6 0 139 204 218 714.6 0 218 205 214 714.6 0 214 206 247 714.6 0 247 207 239 714.6 0 239 208 324 714.6 0 324 209 291 714.6 0 291 210 382 714.6 0 382 211 516 714.6 0 516 212 1325 714.6 0 1325 213 1638 714.6 0 1638 214 2317 714.6 0 2317 215 366 714.6 0 366 216 509 714.6 0 509 217 549 714.6 0 549 218 529 714.6 0 529 219 297 714.6 0 297 220 372 714.6 0 372 221 427 714.6 0 427 222 405 714.6 0 405 223 376 714.6 0 376 224 427 714.6 0 427 225 723 714.6 0 723 226 488 714.6 0 488 227 493 714.6 0 493 228 384 714.6 0 384 229 501 714.6 0 501 230 405 714.6 0 405 231 315 714.6 0 315 232 311 714.6 0 311 233 375 714.6 0 375 234 331 714.6 0 331 235 278 714.6 0 278 236 405 714.6 0 405 237 500 714.6 0 500 238 478 714.6 0 478 239 744 714.6 0 744 240 746 714.6 0 746 241 805 714.6 0 805 242 517 714.6 0 517 243 678 714.6 0 678 244 499 714.6 0 499 245 766 714.6 0 766 246 594 714.6 0 594 247 518 714.6 0 518 248 308 714.6 0 308 249 300 714.6 0 300 250 560 714.6 0 560 251 378 714.6 0 378 252 245 714.6 0 245 253 383 714.6 0 383 254 734 714.6 0 734 255 776 714.6 0 776 256 1102 714.6 0 1102 257 1115 714.6 0 1115 258 648 714.6 0 648 259 364 714.6 0 364 260 573 714.6 0 573 261 535 714.6 0 535 262 515 714.6 0 515 263 458 714.6 0 458 264 560 714.6 0 560 265 469 714.6 0 469 266 1400 714.6 0 1400 267 826 714.6 0 826 268 666 714.6 0 666 269 551 714.6 0 551 270 619 714.6 0 619 271 496 714.6 0 496 272 488 714.6 0 488 273 980 714.6 0 980 274 892 714.6 0 892 275 754 714.6 0 754 276 1094 714.6 0 1094 277 789 714.6 0 789 278 614 714.6 0 614 279 434 714.6 0 434 280 500 714.6 0 500 281 779 714.6 0 779 282 1171 714.6 0 1171 283 420 714.6 0 420 284 555 714.6 0 555 285 576 714.6 0 576 286 393 714.6 0 393 287 624 714.6 0 624 288 597 714.6 0 597 289 946 714.6 0 946 290 418 714.6 0 418 291 261 714.6 0 261 292 160 714.6 0 160 293 181 714.6 0 181 294 157 714.6 0 157 295 149 714.6 0 149 296 132 714.6 0 132 297 197 714.6 0 197 298 301 714.6 0 301 299 257 714.6 0 257 300 313 714.6 0 313 301 250 714.6 0 250 Finished in 2.74 s (38 us/read; 1.56 M reads/minute). === Summary === Total reads processed: 71,396 Reads with adapters: 10,683 (15.0%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 10,664 (14.9%) Total basepairs processed: 12,740,610 bp Total written (filtered): 2,920,936 bp (22.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 10683 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 5 0.0 3 0 0 3 0 2 19 155 0.0 3 0 151 2 1 1 20 9806 0.0 4 9668 133 3 1 1 21 679 0.0 4 641 37 1 22 4 0.0 4 1 1 2 23 2 0.0 4 0 0 0 2 29 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 62 5 0.0 4 0 0 0 1 4 64 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 1 105 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 107 2 0.0 4 0 0 0 1 1 111 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 1 119 1 0.0 4 0 0 0 0 1 128 7 0.0 4 0 0 0 0 7 131 1 0.0 4 0 0 0 1 159 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.36 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 10,664 Reads with adapters: 168 (1.6%) Reads written (passing filters): 10,664 (100.0%) Total basepairs processed: 2,920,936 bp Total written (filtered): 2,917,408 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 168 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.4% C: 0.0% G: 94.0% T: 0.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 166.6 0 1 4 1 41.7 0 1 5 1 10.4 0 1 11 157 0.0 1 0 157 12 1 0.0 1 0 1 211 1 0.0 1 0 1 261 6 0.0 1 6 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.