This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.97 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 84,425 (2.9%) Reads that were too short: 29,172 (1.0%) Reads written (passing filters): 55,253 (1.9%) Total basepairs processed: 881,011,047 bp Total written (filtered): 8,405,591 bp (1.0%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 84425 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 187 714.6 0 187 7 54 714.6 0 54 8 137 714.6 0 137 9 1564 714.6 0 1564 10 24 714.6 0 24 11 24 714.6 0 24 12 16 714.6 0 16 13 25 714.6 0 25 14 21 714.6 0 21 15 23 714.6 0 23 16 31 714.6 0 31 17 29 714.6 0 29 18 34 714.6 0 34 19 37 714.6 0 37 20 46 714.6 0 46 21 242 714.6 0 242 22 56 714.6 0 56 23 35 714.6 0 35 24 29 714.6 0 29 25 40 714.6 0 40 26 1551 714.6 0 1551 27 309 714.6 0 309 28 30 714.6 0 30 29 34 714.6 0 34 30 39 714.6 0 39 31 27 714.6 0 27 32 37 714.6 0 37 33 139 714.6 0 139 34 73 714.6 0 73 35 42 714.6 0 42 36 30 714.6 0 30 37 27 714.6 0 27 38 36 714.6 0 36 39 29 714.6 0 29 40 31 714.6 0 31 41 33 714.6 0 33 42 32 714.6 0 32 43 29 714.6 0 29 44 48 714.6 0 48 45 32 714.6 0 32 46 26 714.6 0 26 47 46 714.6 0 46 48 23 714.6 0 23 49 22 714.6 0 22 50 32 714.6 0 32 51 23 714.6 0 23 52 31 714.6 0 31 53 41 714.6 0 41 54 27 714.6 0 27 55 32 714.6 0 32 56 23 714.6 0 23 57 29 714.6 0 29 58 37 714.6 0 37 59 31 714.6 0 31 60 41 714.6 0 41 61 26 714.6 0 26 62 26 714.6 0 26 63 34 714.6 0 34 64 41 714.6 0 41 65 50 714.6 0 50 66 30 714.6 0 30 67 28 714.6 0 28 68 38 714.6 0 38 69 107 714.6 0 107 70 34 714.6 0 34 71 37 714.6 0 37 72 24 714.6 0 24 73 19 714.6 0 19 74 54 714.6 0 54 75 60 714.6 0 60 76 78 714.6 0 78 77 64 714.6 0 64 78 56 714.6 0 56 79 89 714.6 0 89 80 318 714.6 0 318 81 193 714.6 0 193 82 192 714.6 0 192 83 176 714.6 0 176 84 192 714.6 0 192 85 93 714.6 0 93 86 177 714.6 0 177 87 161 714.6 0 161 88 204 714.6 0 204 89 153 714.6 0 153 90 117 714.6 0 117 91 75 714.6 0 75 92 67 714.6 0 67 93 127 714.6 0 127 94 105 714.6 0 105 95 165 714.6 0 165 96 274 714.6 0 274 97 339 714.6 0 339 98 114 714.6 0 114 99 115 714.6 0 115 100 139 714.6 0 139 101 97 714.6 0 97 102 85 714.6 0 85 103 78 714.6 0 78 104 58 714.6 0 58 105 59 714.6 0 59 106 43 714.6 0 43 107 45 714.6 0 45 108 222 714.6 0 222 109 28 714.6 0 28 110 47 714.6 0 47 111 81 714.6 0 81 112 80 714.6 0 80 113 140 714.6 0 140 114 116 714.6 0 116 115 91 714.6 0 91 116 104 714.6 0 104 117 586 714.6 0 586 118 408 714.6 0 408 119 281 714.6 0 281 120 103 714.6 0 103 121 121 714.6 0 121 122 131 714.6 0 131 123 120 714.6 0 120 124 100 714.6 0 100 125 96 714.6 0 96 126 123 714.6 0 123 127 152 714.6 0 152 128 138 714.6 0 138 129 121 714.6 0 121 130 131 714.6 0 131 131 218 714.6 0 218 132 197 714.6 0 197 133 283 714.6 0 283 134 179 714.6 0 179 135 153 714.6 0 153 136 200 714.6 0 200 137 173 714.6 0 173 138 141 714.6 0 141 139 185 714.6 0 185 140 164 714.6 0 164 141 122 714.6 0 122 142 129 714.6 0 129 143 132 714.6 0 132 144 82 714.6 0 82 145 112 714.6 0 112 146 148 714.6 0 148 147 152 714.6 0 152 148 126 714.6 0 126 149 109 714.6 0 109 150 109 714.6 0 109 151 83 714.6 0 83 152 98 714.6 0 98 153 115 714.6 0 115 154 118 714.6 0 118 155 101 714.6 0 101 156 265 714.6 0 265 157 197 714.6 0 197 158 387 714.6 0 387 159 123 714.6 0 123 160 144 714.6 0 144 161 152 714.6 0 152 162 175 714.6 0 175 163 198 714.6 0 198 164 155 714.6 0 155 165 293 714.6 0 293 166 173 714.6 0 173 167 211 714.6 0 211 168 255 714.6 0 255 169 257 714.6 0 257 170 220 714.6 0 220 171 225 714.6 0 225 172 190 714.6 0 190 173 197 714.6 0 197 174 252 714.6 0 252 175 426 714.6 0 426 176 594 714.6 0 594 177 1260 714.6 0 1260 178 2116 714.6 0 2116 179 17631 714.6 0 17631 180 1435 714.6 0 1435 181 1723 714.6 0 1723 182 1421 714.6 0 1421 183 583 714.6 0 583 184 1874 714.6 0 1874 185 361 714.6 0 361 186 141 714.6 0 141 187 171 714.6 0 171 188 218 714.6 0 218 189 146 714.6 0 146 190 84 714.6 0 84 191 90 714.6 0 90 192 117 714.6 0 117 193 125 714.6 0 125 194 300 714.6 0 300 195 470 714.6 0 470 196 664 714.6 0 664 197 443 714.6 0 443 198 310 714.6 0 310 199 160 714.6 0 160 200 269 714.6 0 269 201 321 714.6 0 321 202 333 714.6 0 333 203 321 714.6 0 321 204 171 714.6 0 171 205 120 714.6 0 120 206 105 714.6 0 105 207 134 714.6 0 134 208 127 714.6 0 127 209 115 714.6 0 115 210 99 714.6 0 99 211 88 714.6 0 88 212 107 714.6 0 107 213 141 714.6 0 141 214 143 714.6 0 143 215 161 714.6 0 161 216 173 714.6 0 173 217 136 714.6 0 136 218 90 714.6 0 90 219 100 714.6 0 100 220 175 714.6 0 175 221 114 714.6 0 114 222 113 714.6 0 113 223 147 714.6 0 147 224 200 714.6 0 200 225 163 714.6 0 163 226 155 714.6 0 155 227 109 714.6 0 109 228 127 714.6 0 127 229 209 714.6 0 209 230 144 714.6 0 144 231 112 714.6 0 112 232 111 714.6 0 111 233 96 714.6 0 96 234 93 714.6 0 93 235 121 714.6 0 121 236 157 714.6 0 157 237 116 714.6 0 116 238 224 714.6 0 224 239 1821 714.6 0 1821 240 393 714.6 0 393 241 717 714.6 0 717 242 106 714.6 0 106 243 139 714.6 0 139 244 105 714.6 0 105 245 96 714.6 0 96 246 128 714.6 0 128 247 153 714.6 0 153 248 195 714.6 0 195 249 204 714.6 0 204 250 145 714.6 0 145 251 93 714.6 0 93 252 100 714.6 0 100 253 104 714.6 0 104 254 152 714.6 0 152 255 99 714.6 0 99 256 136 714.6 0 136 257 189 714.6 0 189 258 318 714.6 0 318 259 643 714.6 0 643 260 1143 714.6 0 1143 261 1384 714.6 0 1384 262 293 714.6 0 293 263 371 714.6 0 371 264 883 714.6 0 883 265 1201 714.6 0 1201 266 1271 714.6 0 1271 267 755 714.6 0 755 268 161 714.6 0 161 269 145 714.6 0 145 270 84 714.6 0 84 271 38 714.6 0 38 272 44 714.6 0 44 273 53 714.6 0 53 274 115 714.6 0 115 275 182 714.6 0 182 276 129 714.6 0 129 277 70 714.6 0 70 278 73 714.6 0 73 279 135 714.6 0 135 280 135 714.6 0 135 281 152 714.6 0 152 282 82 714.6 0 82 283 65 714.6 0 65 284 70 714.6 0 70 285 54 714.6 0 54 286 117 714.6 0 117 287 198 714.6 0 198 288 162 714.6 0 162 289 156 714.6 0 156 290 94 714.6 0 94 291 490 714.6 0 490 292 2483 714.6 0 2483 293 2287 714.6 0 2287 294 877 714.6 0 877 295 914 714.6 0 914 296 279 714.6 0 279 297 208 714.6 0 208 298 97 714.6 0 97 299 94 714.6 0 94 300 162 714.6 0 162 301 80 714.6 0 80 Finished in 1.72 s (31 us/read; 1.93 M reads/minute). === Summary === Total reads processed: 55,253 Reads with adapters: 304 (0.6%) Reads that were too short: 80 (0.1%) Reads written (passing filters): 224 (0.4%) Total basepairs processed: 8,405,591 bp Total written (filtered): 56,007 bp (0.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 304 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 0 0 2 17 17 0.0 3 11 3 3 18 2 0.0 3 0 0 1 1 19 10 0.0 3 2 4 0 4 20 160 0.0 3 154 5 0 1 22 1 0.0 3 0 0 1 27 2 0.0 3 0 0 0 2 31 1 0.0 3 0 0 0 1 35 6 0.0 3 0 0 0 6 36 1 0.0 3 0 0 0 1 41 3 0.0 3 0 0 0 3 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 50 34 0.0 3 0 0 0 34 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 3 0.0 3 0 0 0 3 61 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 1 100 16 0.0 3 0 0 0 16 102 3 0.0 3 0 0 0 3 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 3 0.0 3 0 0 0 3 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 149 3 0.0 3 0 0 0 3 159 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 224 Reads with adapters: 8 (3.6%) Reads written (passing filters): 224 (100.0%) Total basepairs processed: 56,007 bp Total written (filtered): 55,983 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 8 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.0% C: 37.5% G: 12.5% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 8 3.5 0 8