This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AACGCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.48 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 41,392 (1.4%) Reads that were too short: 23,909 (0.8%) Reads written (passing filters): 17,483 (0.6%) Total basepairs processed: 881,011,047 bp Total written (filtered): 3,120,069 bp (0.4%) === Adapter 1 === Sequence: AACGCG; Type: regular 5'; Length: 6; Trimmed: 41392 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 59 714.6 0 59 7 88 714.6 0 88 8 39 714.6 0 39 9 78 714.6 0 78 10 47 714.6 0 47 11 66 714.6 0 66 12 66 714.6 0 66 13 67 714.6 0 67 14 61 714.6 0 61 15 57 714.6 0 57 16 77 714.6 0 77 17 54 714.6 0 54 18 70 714.6 0 70 19 51 714.6 0 51 20 62 714.6 0 62 21 68 714.6 0 68 22 55 714.6 0 55 23 62 714.6 0 62 24 54 714.6 0 54 25 55 714.6 0 55 26 54 714.6 0 54 27 55 714.6 0 55 28 54 714.6 0 54 29 44 714.6 0 44 30 65 714.6 0 65 31 62 714.6 0 62 32 58 714.6 0 58 33 64 714.6 0 64 34 59 714.6 0 59 35 68 714.6 0 68 36 41 714.6 0 41 37 51 714.6 0 51 38 66 714.6 0 66 39 46 714.6 0 46 40 63 714.6 0 63 41 55 714.6 0 55 42 52 714.6 0 52 43 65 714.6 0 65 44 103 714.6 0 103 45 67 714.6 0 67 46 109 714.6 0 109 47 63 714.6 0 63 48 59 714.6 0 59 49 55 714.6 0 55 50 47 714.6 0 47 51 56 714.6 0 56 52 53 714.6 0 53 53 62 714.6 0 62 54 59 714.6 0 59 55 77 714.6 0 77 56 45 714.6 0 45 57 53 714.6 0 53 58 61 714.6 0 61 59 53 714.6 0 53 60 47 714.6 0 47 61 47 714.6 0 47 62 71 714.6 0 71 63 43 714.6 0 43 64 52 714.6 0 52 65 64 714.6 0 64 66 54 714.6 0 54 67 61 714.6 0 61 68 48 714.6 0 48 69 43 714.6 0 43 70 46 714.6 0 46 71 82 714.6 0 82 72 78 714.6 0 78 73 50 714.6 0 50 74 47 714.6 0 47 75 55 714.6 0 55 76 37 714.6 0 37 77 49 714.6 0 49 78 54 714.6 0 54 79 61 714.6 0 61 80 68 714.6 0 68 81 86 714.6 0 86 82 80 714.6 0 80 83 70 714.6 0 70 84 77 714.6 0 77 85 67 714.6 0 67 86 50 714.6 0 50 87 39 714.6 0 39 88 52 714.6 0 52 89 61 714.6 0 61 90 64 714.6 0 64 91 80 714.6 0 80 92 117 714.6 0 117 93 145 714.6 0 145 94 134 714.6 0 134 95 64 714.6 0 64 96 75 714.6 0 75 97 71 714.6 0 71 98 53 714.6 0 53 99 80 714.6 0 80 100 95 714.6 0 95 101 77 714.6 0 77 102 60 714.6 0 60 103 34 714.6 0 34 104 50 714.6 0 50 105 48 714.6 0 48 106 48 714.6 0 48 107 51 714.6 0 51 108 67 714.6 0 67 109 70 714.6 0 70 110 74 714.6 0 74 111 90 714.6 0 90 112 67 714.6 0 67 113 81 714.6 0 81 114 72 714.6 0 72 115 107 714.6 0 107 116 87 714.6 0 87 117 115 714.6 0 115 118 81 714.6 0 81 119 94 714.6 0 94 120 82 714.6 0 82 121 101 714.6 0 101 122 118 714.6 0 118 123 105 714.6 0 105 124 200 714.6 0 200 125 144 714.6 0 144 126 106 714.6 0 106 127 140 714.6 0 140 128 172 714.6 0 172 129 106 714.6 0 106 130 86 714.6 0 86 131 112 714.6 0 112 132 103 714.6 0 103 133 74 714.6 0 74 134 144 714.6 0 144 135 152 714.6 0 152 136 143 714.6 0 143 137 98 714.6 0 98 138 81 714.6 0 81 139 85 714.6 0 85 140 92 714.6 0 92 141 88 714.6 0 88 142 57 714.6 0 57 143 87 714.6 0 87 144 122 714.6 0 122 145 121 714.6 0 121 146 95 714.6 0 95 147 72 714.6 0 72 148 62 714.6 0 62 149 75 714.6 0 75 150 99 714.6 0 99 151 85 714.6 0 85 152 73 714.6 0 73 153 94 714.6 0 94 154 106 714.6 0 106 155 87 714.6 0 87 156 94 714.6 0 94 157 104 714.6 0 104 158 101 714.6 0 101 159 127 714.6 0 127 160 81 714.6 0 81 161 77 714.6 0 77 162 85 714.6 0 85 163 100 714.6 0 100 164 126 714.6 0 126 165 98 714.6 0 98 166 111 714.6 0 111 167 118 714.6 0 118 168 95 714.6 0 95 169 103 714.6 0 103 170 108 714.6 0 108 171 148 714.6 0 148 172 163 714.6 0 163 173 120 714.6 0 120 174 106 714.6 0 106 175 96 714.6 0 96 176 137 714.6 0 137 177 151 714.6 0 151 178 202 714.6 0 202 179 170 714.6 0 170 180 140 714.6 0 140 181 109 714.6 0 109 182 227 714.6 0 227 183 195 714.6 0 195 184 175 714.6 0 175 185 80 714.6 0 80 186 95 714.6 0 95 187 76 714.6 0 76 188 168 714.6 0 168 189 231 714.6 0 231 190 276 714.6 0 276 191 186 714.6 0 186 192 143 714.6 0 143 193 76 714.6 0 76 194 87 714.6 0 87 195 91 714.6 0 91 196 225 714.6 0 225 197 400 714.6 0 400 198 306 714.6 0 306 199 111 714.6 0 111 200 68 714.6 0 68 201 60 714.6 0 60 202 135 714.6 0 135 203 171 714.6 0 171 204 130 714.6 0 130 205 94 714.6 0 94 206 84 714.6 0 84 207 80 714.6 0 80 208 87 714.6 0 87 209 97 714.6 0 97 210 82 714.6 0 82 211 115 714.6 0 115 212 144 714.6 0 144 213 126 714.6 0 126 214 125 714.6 0 125 215 140 714.6 0 140 216 136 714.6 0 136 217 221 714.6 0 221 218 148 714.6 0 148 219 270 714.6 0 270 220 404 714.6 0 404 221 799 714.6 0 799 222 395 714.6 0 395 223 282 714.6 0 282 224 176 714.6 0 176 225 176 714.6 0 176 226 170 714.6 0 170 227 153 714.6 0 153 228 135 714.6 0 135 229 104 714.6 0 104 230 103 714.6 0 103 231 79 714.6 0 79 232 111 714.6 0 111 233 107 714.6 0 107 234 129 714.6 0 129 235 127 714.6 0 127 236 274 714.6 0 274 237 198 714.6 0 198 238 194 714.6 0 194 239 144 714.6 0 144 240 119 714.6 0 119 241 93 714.6 0 93 242 118 714.6 0 118 243 147 714.6 0 147 244 554 714.6 0 554 245 554 714.6 0 554 246 850 714.6 0 850 247 290 714.6 0 290 248 95 714.6 0 95 249 73 714.6 0 73 250 106 714.6 0 106 251 173 714.6 0 173 252 214 714.6 0 214 253 166 714.6 0 166 254 136 714.6 0 136 255 166 714.6 0 166 256 136 714.6 0 136 257 85 714.6 0 85 258 79 714.6 0 79 259 60 714.6 0 60 260 71 714.6 0 71 261 108 714.6 0 108 262 98 714.6 0 98 263 141 714.6 0 141 264 153 714.6 0 153 265 138 714.6 0 138 266 107 714.6 0 107 267 156 714.6 0 156 268 205 714.6 0 205 269 127 714.6 0 127 270 188 714.6 0 188 271 194 714.6 0 194 272 158 714.6 0 158 273 115 714.6 0 115 274 107 714.6 0 107 275 108 714.6 0 108 276 86 714.6 0 86 277 92 714.6 0 92 278 121 714.6 0 121 279 100 714.6 0 100 280 95 714.6 0 95 281 79 714.6 0 79 282 131 714.6 0 131 283 222 714.6 0 222 284 167 714.6 0 167 285 135 714.6 0 135 286 142 714.6 0 142 287 300 714.6 0 300 288 1250 714.6 0 1250 289 2234 714.6 0 2234 290 4205 714.6 0 4205 291 628 714.6 0 628 292 110 714.6 0 110 293 73 714.6 0 73 294 36 714.6 0 36 295 35 714.6 0 35 296 121 714.6 0 121 297 87 714.6 0 87 298 86 714.6 0 86 299 141 714.6 0 141 300 160 714.6 0 160 301 40 714.6 0 40 Finished in 0.67 s (38 us/read; 1.57 M reads/minute). === Summary === Total reads processed: 17,483 Reads with adapters: 20 (0.1%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 14 (0.1%) Total basepairs processed: 3,120,069 bp Total written (filtered): 3,520 bp (0.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 20 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 20 3 0.0 4 3 21 7 0.0 4 7 22 1 0.0 4 1 32 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 1 92 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (714 us/read; 0.08 M reads/minute). === Summary === Total reads processed: 14 Reads with adapters: 0 (0.0%) Reads written (passing filters): 14 (100.0%) Total basepairs processed: 3,520 bp Total written (filtered): 3,520 bp (100.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 0 times.