This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATAATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 89.93 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 351,458 (10.1%) Reads that were too short: 249,555 (7.2%) Reads written (passing filters): 101,903 (2.9%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 17,550,407 bp (1.7%) === Adapter 1 === Sequence: ATAATT; Type: regular 5'; Length: 6; Trimmed: 351458 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 52 846.0 0 52 7 86 846.0 0 86 8 68 846.0 0 68 9 88 846.0 0 88 10 52 846.0 0 52 11 49 846.0 0 49 12 58 846.0 0 58 13 56 846.0 0 56 14 55 846.0 0 55 15 51 846.0 0 51 16 51 846.0 0 51 17 50 846.0 0 50 18 45 846.0 0 45 19 45 846.0 0 45 20 47 846.0 0 47 21 46 846.0 0 46 22 48 846.0 0 48 23 46 846.0 0 46 24 47 846.0 0 47 25 54 846.0 0 54 26 47 846.0 0 47 27 55 846.0 0 55 28 66 846.0 0 66 29 66 846.0 0 66 30 64 846.0 0 64 31 54 846.0 0 54 32 58 846.0 0 58 33 54 846.0 0 54 34 62 846.0 0 62 35 95 846.0 0 95 36 176 846.0 0 176 37 103 846.0 0 103 38 102 846.0 0 102 39 53 846.0 0 53 40 52 846.0 0 52 41 49 846.0 0 49 42 64 846.0 0 64 43 42 846.0 0 42 44 60 846.0 0 60 45 51 846.0 0 51 46 34 846.0 0 34 47 35 846.0 0 35 48 37 846.0 0 37 49 34 846.0 0 34 50 33 846.0 0 33 51 40 846.0 0 40 52 35 846.0 0 35 53 35 846.0 0 35 54 48 846.0 0 48 55 45 846.0 0 45 56 4311 846.0 0 4311 57 552 846.0 0 552 58 45 846.0 0 45 59 43 846.0 0 43 60 59 846.0 0 59 61 35 846.0 0 35 62 50 846.0 0 50 63 43 846.0 0 43 64 49 846.0 0 49 65 121 846.0 0 121 66 95 846.0 0 95 67 58 846.0 0 58 68 70 846.0 0 70 69 164 846.0 0 164 70 142 846.0 0 142 71 185 846.0 0 185 72 373 846.0 0 373 73 316 846.0 0 316 74 348 846.0 0 348 75 689 846.0 0 689 76 522 846.0 0 522 77 944 846.0 0 944 78 1083 846.0 0 1083 79 451 846.0 0 451 80 969 846.0 0 969 81 868 846.0 0 868 82 521 846.0 0 521 83 407 846.0 0 407 84 479 846.0 0 479 85 285 846.0 0 285 86 436 846.0 0 436 87 511 846.0 0 511 88 593 846.0 0 593 89 457 846.0 0 457 90 756 846.0 0 756 91 349 846.0 0 349 92 516 846.0 0 516 93 519 846.0 0 519 94 472 846.0 0 472 95 521 846.0 0 521 96 675 846.0 0 675 97 921 846.0 0 921 98 2957 846.0 0 2957 99 1376 846.0 0 1376 100 536 846.0 0 536 101 757 846.0 0 757 102 409 846.0 0 409 103 188 846.0 0 188 104 177 846.0 0 177 105 383 846.0 0 383 106 1346 846.0 0 1346 107 1004 846.0 0 1004 108 931 846.0 0 931 109 627 846.0 0 627 110 1019 846.0 0 1019 111 1007 846.0 0 1007 112 964 846.0 0 964 113 698 846.0 0 698 114 722 846.0 0 722 115 452 846.0 0 452 116 332 846.0 0 332 117 308 846.0 0 308 118 325 846.0 0 325 119 1050 846.0 0 1050 120 980 846.0 0 980 121 526 846.0 0 526 122 415 846.0 0 415 123 1285 846.0 0 1285 124 562 846.0 0 562 125 528 846.0 0 528 126 501 846.0 0 501 127 520 846.0 0 520 128 710 846.0 0 710 129 654 846.0 0 654 130 471 846.0 0 471 131 463 846.0 0 463 132 717 846.0 0 717 133 594 846.0 0 594 134 677 846.0 0 677 135 432 846.0 0 432 136 381 846.0 0 381 137 413 846.0 0 413 138 565 846.0 0 565 139 1754 846.0 0 1754 140 1280 846.0 0 1280 141 662 846.0 0 662 142 323 846.0 0 323 143 381 846.0 0 381 144 321 846.0 0 321 145 366 846.0 0 366 146 591 846.0 0 591 147 446 846.0 0 446 148 476 846.0 0 476 149 387 846.0 0 387 150 369 846.0 0 369 151 277 846.0 0 277 152 290 846.0 0 290 153 381 846.0 0 381 154 410 846.0 0 410 155 495 846.0 0 495 156 520 846.0 0 520 157 509 846.0 0 509 158 614 846.0 0 614 159 9791 846.0 0 9791 160 5410 846.0 0 5410 161 6731 846.0 0 6731 162 311 846.0 0 311 163 359 846.0 0 359 164 359 846.0 0 359 165 514 846.0 0 514 166 358 846.0 0 358 167 404 846.0 0 404 168 407 846.0 0 407 169 256 846.0 0 256 170 249 846.0 0 249 171 231 846.0 0 231 172 297 846.0 0 297 173 470 846.0 0 470 174 775 846.0 0 775 175 331 846.0 0 331 176 382 846.0 0 382 177 259 846.0 0 259 178 295 846.0 0 295 179 282 846.0 0 282 180 410 846.0 0 410 181 391 846.0 0 391 182 306 846.0 0 306 183 444 846.0 0 444 184 462 846.0 0 462 185 539 846.0 0 539 186 306 846.0 0 306 187 355 846.0 0 355 188 325 846.0 0 325 189 392 846.0 0 392 190 349 846.0 0 349 191 385 846.0 0 385 192 450 846.0 0 450 193 443 846.0 0 443 194 555 846.0 0 555 195 672 846.0 0 672 196 566 846.0 0 566 197 324 846.0 0 324 198 276 846.0 0 276 199 333 846.0 0 333 200 679 846.0 0 679 201 810 846.0 0 810 202 576 846.0 0 576 203 720 846.0 0 720 204 712 846.0 0 712 205 618 846.0 0 618 206 898 846.0 0 898 207 1265 846.0 0 1265 208 899 846.0 0 899 209 921 846.0 0 921 210 811 846.0 0 811 211 824 846.0 0 824 212 1336 846.0 0 1336 213 575 846.0 0 575 214 603 846.0 0 603 215 411 846.0 0 411 216 353 846.0 0 353 217 232 846.0 0 232 218 400 846.0 0 400 219 519 846.0 0 519 220 638 846.0 0 638 221 886 846.0 0 886 222 499 846.0 0 499 223 524 846.0 0 524 224 679 846.0 0 679 225 452 846.0 0 452 226 294 846.0 0 294 227 408 846.0 0 408 228 567 846.0 0 567 229 597 846.0 0 597 230 552 846.0 0 552 231 578 846.0 0 578 232 1378 846.0 0 1378 233 1265 846.0 0 1265 234 1497 846.0 0 1497 235 451 846.0 0 451 236 434 846.0 0 434 237 556 846.0 0 556 238 713 846.0 0 713 239 408 846.0 0 408 240 867 846.0 0 867 241 794 846.0 0 794 242 861 846.0 0 861 243 758 846.0 0 758 244 465 846.0 0 465 245 360 846.0 0 360 246 208 846.0 0 208 247 248 846.0 0 248 248 400 846.0 0 400 249 705 846.0 0 705 250 1330 846.0 0 1330 251 1287 846.0 0 1287 252 1295 846.0 0 1295 253 2643 846.0 0 2643 254 5820 846.0 0 5820 255 70751 846.0 0 70751 256 81044 846.0 0 81044 257 28832 846.0 0 28832 258 1237 846.0 0 1237 259 505 846.0 0 505 260 1027 846.0 0 1027 261 3154 846.0 0 3154 262 1332 846.0 0 1332 263 753 846.0 0 753 264 359 846.0 0 359 265 380 846.0 0 380 266 297 846.0 0 297 267 214 846.0 0 214 268 319 846.0 0 319 269 459 846.0 0 459 270 453 846.0 0 453 271 492 846.0 0 492 272 491 846.0 0 491 273 439 846.0 0 439 274 235 846.0 0 235 275 348 846.0 0 348 276 448 846.0 0 448 277 361 846.0 0 361 278 285 846.0 0 285 279 314 846.0 0 314 280 331 846.0 0 331 281 348 846.0 0 348 282 713 846.0 0 713 283 934 846.0 0 934 284 655 846.0 0 655 285 192 846.0 0 192 286 151 846.0 0 151 287 242 846.0 0 242 288 344 846.0 0 344 289 443 846.0 0 443 290 678 846.0 0 678 291 314 846.0 0 314 292 322 846.0 0 322 293 310 846.0 0 310 294 292 846.0 0 292 295 440 846.0 0 440 296 580 846.0 0 580 297 611 846.0 0 611 298 562 846.0 0 562 299 691 846.0 0 691 300 1189 846.0 0 1189 301 594 846.0 0 594 Finished in 3.39 s (33 us/read; 1.80 M reads/minute). === Summary === Total reads processed: 101,903 Reads with adapters: 84 (0.1%) Reads that were too short: 30 (0.0%) Reads written (passing filters): 54 (0.1%) Total basepairs processed: 17,550,407 bp Total written (filtered): 9,081 bp (0.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 84 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 17 16 0.0 3 14 2 26 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 2 0.0 3 0 0 0 2 51 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 63 3 0.0 3 0 0 0 3 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 86 2 0.0 3 0 0 0 2 87 2 0.0 3 0 0 0 2 89 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 114 2 0.0 3 0 0 0 2 115 5 0.0 3 0 0 0 5 116 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 3 0.0 3 0 0 0 3 145 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (185 us/read; 0.32 M reads/minute). === Summary === Total reads processed: 54 Reads with adapters: 6 (11.1%) Reads written (passing filters): 54 (100.0%) Total basepairs processed: 9,081 bp Total written (filtered): 9,062 bp (99.8%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 6 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 83.3% C: 16.7% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 5 0.8 0 5 4 1 0.2 0 1