This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCTAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 91.59 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 95,436 (2.8%) Reads that were too short: 50,592 (1.5%) Reads written (passing filters): 44,844 (1.3%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 8,082,188 bp (0.8%) === Adapter 1 === Sequence: GGCTAC; Type: regular 5'; Length: 6; Trimmed: 95436 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 6 846.0 0 6 7 52 846.0 0 52 8 38 846.0 0 38 9 8 846.0 0 8 10 12 846.0 0 12 11 16 846.0 0 16 12 25 846.0 0 25 13 134 846.0 0 134 14 330 846.0 0 330 15 158 846.0 0 158 16 35 846.0 0 35 17 12 846.0 0 12 18 15 846.0 0 15 19 15 846.0 0 15 20 6 846.0 0 6 21 5 846.0 0 5 22 7 846.0 0 7 23 10 846.0 0 10 24 4 846.0 0 4 25 7 846.0 0 7 26 5 846.0 0 5 27 10 846.0 0 10 28 7 846.0 0 7 29 52 846.0 0 52 30 10 846.0 0 10 31 32 846.0 0 32 32 12 846.0 0 12 33 15 846.0 0 15 34 12 846.0 0 12 35 72 846.0 0 72 36 33 846.0 0 33 37 53 846.0 0 53 38 1827 846.0 0 1827 39 123 846.0 0 123 40 40 846.0 0 40 41 9 846.0 0 9 42 7 846.0 0 7 43 8 846.0 0 8 44 11 846.0 0 11 45 15 846.0 0 15 46 9 846.0 0 9 47 8 846.0 0 8 48 4 846.0 0 4 49 30 846.0 0 30 50 37 846.0 0 37 51 137 846.0 0 137 52 8 846.0 0 8 53 17 846.0 0 17 54 19 846.0 0 19 55 15 846.0 0 15 56 11 846.0 0 11 57 13 846.0 0 13 58 22 846.0 0 22 59 257 846.0 0 257 60 6 846.0 0 6 61 9 846.0 0 9 62 11 846.0 0 11 63 6 846.0 0 6 64 2 846.0 0 2 65 15 846.0 0 15 66 10 846.0 0 10 67 3 846.0 0 3 68 10 846.0 0 10 69 10 846.0 0 10 70 5 846.0 0 5 71 7 846.0 0 7 72 5 846.0 0 5 73 5 846.0 0 5 74 18 846.0 0 18 75 8 846.0 0 8 76 9 846.0 0 9 77 6 846.0 0 6 78 7 846.0 0 7 79 9 846.0 0 9 80 8 846.0 0 8 81 11 846.0 0 11 82 16 846.0 0 16 83 41 846.0 0 41 84 80 846.0 0 80 85 70 846.0 0 70 86 10 846.0 0 10 87 25 846.0 0 25 88 21 846.0 0 21 89 21 846.0 0 21 90 8 846.0 0 8 91 5 846.0 0 5 92 10 846.0 0 10 93 5 846.0 0 5 94 18 846.0 0 18 95 9 846.0 0 9 96 19 846.0 0 19 97 11 846.0 0 11 98 12 846.0 0 12 99 22 846.0 0 22 100 34 846.0 0 34 101 25 846.0 0 25 102 36 846.0 0 36 103 18 846.0 0 18 104 39 846.0 0 39 105 31 846.0 0 31 106 24 846.0 0 24 107 21 846.0 0 21 108 43 846.0 0 43 109 83 846.0 0 83 110 214 846.0 0 214 111 201 846.0 0 201 112 43 846.0 0 43 113 66 846.0 0 66 114 133 846.0 0 133 115 190 846.0 0 190 116 119 846.0 0 119 117 95 846.0 0 95 118 97 846.0 0 97 119 103 846.0 0 103 120 112 846.0 0 112 121 166 846.0 0 166 122 252 846.0 0 252 123 630 846.0 0 630 124 18632 846.0 0 18632 125 5280 846.0 0 5280 126 7914 846.0 0 7914 127 68 846.0 0 68 128 56 846.0 0 56 129 45 846.0 0 45 130 68 846.0 0 68 131 26 846.0 0 26 132 19 846.0 0 19 133 119 846.0 0 119 134 24 846.0 0 24 135 26 846.0 0 26 136 10 846.0 0 10 137 15 846.0 0 15 138 18 846.0 0 18 139 23 846.0 0 23 140 27 846.0 0 27 141 34 846.0 0 34 142 25 846.0 0 25 143 26 846.0 0 26 144 30 846.0 0 30 145 38 846.0 0 38 146 32 846.0 0 32 147 186 846.0 0 186 148 279 846.0 0 279 149 282 846.0 0 282 150 28 846.0 0 28 151 34 846.0 0 34 152 458 846.0 0 458 153 103 846.0 0 103 154 119 846.0 0 119 155 60 846.0 0 60 156 64 846.0 0 64 157 244 846.0 0 244 158 118 846.0 0 118 159 259 846.0 0 259 160 116 846.0 0 116 161 99 846.0 0 99 162 131 846.0 0 131 163 21 846.0 0 21 164 26 846.0 0 26 165 26 846.0 0 26 166 25 846.0 0 25 167 18 846.0 0 18 168 35 846.0 0 35 169 41 846.0 0 41 170 30 846.0 0 30 171 34 846.0 0 34 172 63 846.0 0 63 173 61 846.0 0 61 174 69 846.0 0 69 175 25 846.0 0 25 176 65 846.0 0 65 177 851 846.0 0 851 178 283 846.0 0 283 179 303 846.0 0 303 180 99 846.0 0 99 181 14 846.0 0 14 182 19 846.0 0 19 183 24 846.0 0 24 184 10 846.0 0 10 185 20 846.0 0 20 186 14 846.0 0 14 187 21 846.0 0 21 188 25 846.0 0 25 189 22 846.0 0 22 190 62 846.0 0 62 191 14 846.0 0 14 192 17 846.0 0 17 193 22 846.0 0 22 194 51 846.0 0 51 195 18 846.0 0 18 196 24 846.0 0 24 197 12 846.0 0 12 198 12 846.0 0 12 199 13 846.0 0 13 200 10 846.0 0 10 201 17 846.0 0 17 202 14 846.0 0 14 203 6 846.0 0 6 204 14 846.0 0 14 205 11 846.0 0 11 206 13 846.0 0 13 207 37 846.0 0 37 208 32 846.0 0 32 209 23 846.0 0 23 210 11 846.0 0 11 211 17 846.0 0 17 212 26 846.0 0 26 213 24 846.0 0 24 214 35 846.0 0 35 215 21 846.0 0 21 216 25 846.0 0 25 217 18 846.0 0 18 218 15 846.0 0 15 219 28 846.0 0 28 220 29 846.0 0 29 221 32 846.0 0 32 222 16 846.0 0 16 223 18 846.0 0 18 224 16 846.0 0 16 225 19 846.0 0 19 226 10 846.0 0 10 227 19 846.0 0 19 228 29 846.0 0 29 229 23 846.0 0 23 230 27 846.0 0 27 231 54 846.0 0 54 232 25 846.0 0 25 233 252 846.0 0 252 234 92 846.0 0 92 235 159 846.0 0 159 236 23 846.0 0 23 237 19 846.0 0 19 238 18 846.0 0 18 239 33 846.0 0 33 240 33 846.0 0 33 241 37 846.0 0 37 242 19 846.0 0 19 243 18 846.0 0 18 244 29 846.0 0 29 245 24 846.0 0 24 246 23 846.0 0 23 247 35 846.0 0 35 248 24 846.0 0 24 249 35 846.0 0 35 250 19 846.0 0 19 251 15 846.0 0 15 252 18 846.0 0 18 253 27 846.0 0 27 254 36 846.0 0 36 255 44 846.0 0 44 256 37 846.0 0 37 257 200 846.0 0 200 258 206 846.0 0 206 259 1435 846.0 0 1435 260 64 846.0 0 64 261 60 846.0 0 60 262 161 846.0 0 161 263 377 846.0 0 377 264 98 846.0 0 98 265 100 846.0 0 100 266 18 846.0 0 18 267 39 846.0 0 39 268 208 846.0 0 208 269 70 846.0 0 70 270 45 846.0 0 45 271 18 846.0 0 18 272 16 846.0 0 16 273 20 846.0 0 20 274 26 846.0 0 26 275 28 846.0 0 28 276 42 846.0 0 42 277 15 846.0 0 15 278 33 846.0 0 33 279 19 846.0 0 19 280 83 846.0 0 83 281 25 846.0 0 25 282 17 846.0 0 17 283 24 846.0 0 24 284 29 846.0 0 29 285 37 846.0 0 37 286 18 846.0 0 18 287 49 846.0 0 49 288 385 846.0 0 385 289 134 846.0 0 134 290 217 846.0 0 217 291 57 846.0 0 57 292 239 846.0 0 239 293 7976 846.0 0 7976 294 25790 846.0 0 25790 295 9502 846.0 0 9502 296 852 846.0 0 852 297 20 846.0 0 20 298 19 846.0 0 19 299 20 846.0 0 20 300 25 846.0 0 25 301 20 846.0 0 20 Finished in 1.80 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 44,844 Reads with adapters: 39 (0.1%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 34 (0.1%) Total basepairs processed: 8,082,188 bp Total written (filtered): 8,595 bp (0.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 39 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 20 28 0.0 4 28 39 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 63 2 0.0 4 0 0 0 0 2 71 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 128 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (294 us/read; 0.20 M reads/minute). === Summary === Total reads processed: 34 Reads with adapters: 2 (5.9%) Reads written (passing filters): 34 (100.0%) Total basepairs processed: 8,595 bp Total written (filtered): 8,573 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 100.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 11 2 0.0 1 0 2