This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTGCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 89.62 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 249,546 (7.2%) Reads that were too short: 218,955 (6.3%) Reads written (passing filters): 30,591 (0.9%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 7,190,645 bp (0.7%) === Adapter 1 === Sequence: CGTGCG; Type: regular 5'; Length: 6; Trimmed: 249546 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 140 846.0 0 140 7 7463 846.0 0 7463 8 78 846.0 0 78 9 19 846.0 0 19 10 34 846.0 0 34 11 26 846.0 0 26 12 30 846.0 0 30 13 47 846.0 0 47 14 27 846.0 0 27 15 31 846.0 0 31 16 32 846.0 0 32 17 46 846.0 0 46 18 24 846.0 0 24 19 42 846.0 0 42 20 131 846.0 0 131 21 34 846.0 0 34 22 28 846.0 0 28 23 28 846.0 0 28 24 16 846.0 0 16 25 31 846.0 0 31 26 16 846.0 0 16 27 34 846.0 0 34 28 43 846.0 0 43 29 23 846.0 0 23 30 29 846.0 0 29 31 17 846.0 0 17 32 23 846.0 0 23 33 17 846.0 0 17 34 20 846.0 0 20 35 24 846.0 0 24 36 23 846.0 0 23 37 26 846.0 0 26 38 22 846.0 0 22 39 23 846.0 0 23 40 26 846.0 0 26 41 26 846.0 0 26 42 41 846.0 0 41 43 26 846.0 0 26 44 20 846.0 0 20 45 20 846.0 0 20 46 32 846.0 0 32 47 65 846.0 0 65 48 26 846.0 0 26 49 29 846.0 0 29 50 28 846.0 0 28 51 44 846.0 0 44 52 42 846.0 0 42 53 25 846.0 0 25 54 69 846.0 0 69 55 91 846.0 0 91 56 65 846.0 0 65 57 23 846.0 0 23 58 35 846.0 0 35 59 44 846.0 0 44 60 81 846.0 0 81 61 141 846.0 0 141 62 140 846.0 0 140 63 37 846.0 0 37 64 67 846.0 0 67 65 120 846.0 0 120 66 90 846.0 0 90 67 57 846.0 0 57 68 78 846.0 0 78 69 63 846.0 0 63 70 99 846.0 0 99 71 94 846.0 0 94 72 147 846.0 0 147 73 155 846.0 0 155 74 286 846.0 0 286 75 8053 846.0 0 8053 76 3307 846.0 0 3307 77 3952 846.0 0 3952 78 26 846.0 0 26 79 15 846.0 0 15 80 40 846.0 0 40 81 25 846.0 0 25 82 18 846.0 0 18 83 16 846.0 0 16 84 24 846.0 0 24 85 25 846.0 0 25 86 14 846.0 0 14 87 13 846.0 0 13 88 42 846.0 0 42 89 24 846.0 0 24 90 28 846.0 0 28 91 35 846.0 0 35 92 31 846.0 0 31 93 17 846.0 0 17 94 21 846.0 0 21 95 32 846.0 0 32 96 25 846.0 0 25 97 14 846.0 0 14 98 26 846.0 0 26 99 14 846.0 0 14 100 16 846.0 0 16 101 15 846.0 0 15 102 14 846.0 0 14 103 15 846.0 0 15 104 34 846.0 0 34 105 13 846.0 0 13 106 6 846.0 0 6 107 16 846.0 0 16 108 9 846.0 0 9 109 8 846.0 0 8 110 21 846.0 0 21 111 13 846.0 0 13 112 10 846.0 0 10 113 19 846.0 0 19 114 18 846.0 0 18 115 20 846.0 0 20 116 21 846.0 0 21 117 21 846.0 0 21 118 20 846.0 0 20 119 24 846.0 0 24 120 15 846.0 0 15 121 17 846.0 0 17 122 27 846.0 0 27 123 18 846.0 0 18 124 24 846.0 0 24 125 23 846.0 0 23 126 82 846.0 0 82 127 127 846.0 0 127 128 104 846.0 0 104 129 78 846.0 0 78 130 28 846.0 0 28 131 17 846.0 0 17 132 47 846.0 0 47 133 129 846.0 0 129 134 18 846.0 0 18 135 57 846.0 0 57 136 62 846.0 0 62 137 76 846.0 0 76 138 29 846.0 0 29 139 19 846.0 0 19 140 44 846.0 0 44 141 19 846.0 0 19 142 23 846.0 0 23 143 24 846.0 0 24 144 23 846.0 0 23 145 15 846.0 0 15 146 20 846.0 0 20 147 21 846.0 0 21 148 24 846.0 0 24 149 6 846.0 0 6 150 19 846.0 0 19 151 9 846.0 0 9 152 10 846.0 0 10 153 10 846.0 0 10 154 18 846.0 0 18 155 31 846.0 0 31 156 23 846.0 0 23 157 31 846.0 0 31 158 23 846.0 0 23 159 31 846.0 0 31 160 16 846.0 0 16 161 61 846.0 0 61 162 27 846.0 0 27 163 30 846.0 0 30 164 21 846.0 0 21 165 35 846.0 0 35 166 51 846.0 0 51 167 59 846.0 0 59 168 78 846.0 0 78 169 28 846.0 0 28 170 25 846.0 0 25 171 23 846.0 0 23 172 20 846.0 0 20 173 11 846.0 0 11 174 23 846.0 0 23 175 22 846.0 0 22 176 29 846.0 0 29 177 16 846.0 0 16 178 32 846.0 0 32 179 37 846.0 0 37 180 55 846.0 0 55 181 35 846.0 0 35 182 51 846.0 0 51 183 44 846.0 0 44 184 42 846.0 0 42 185 25 846.0 0 25 186 68 846.0 0 68 187 41 846.0 0 41 188 66 846.0 0 66 189 40 846.0 0 40 190 35 846.0 0 35 191 27 846.0 0 27 192 52 846.0 0 52 193 57 846.0 0 57 194 64 846.0 0 64 195 116 846.0 0 116 196 65 846.0 0 65 197 76 846.0 0 76 198 57 846.0 0 57 199 143 846.0 0 143 200 38 846.0 0 38 201 80 846.0 0 80 202 135 846.0 0 135 203 81 846.0 0 81 204 78 846.0 0 78 205 81 846.0 0 81 206 42 846.0 0 42 207 32 846.0 0 32 208 43 846.0 0 43 209 45 846.0 0 45 210 71 846.0 0 71 211 212 846.0 0 212 212 56 846.0 0 56 213 114 846.0 0 114 214 88 846.0 0 88 215 78 846.0 0 78 216 47 846.0 0 47 217 338 846.0 0 338 218 94 846.0 0 94 219 118 846.0 0 118 220 121 846.0 0 121 221 199 846.0 0 199 222 84 846.0 0 84 223 224 846.0 0 224 224 313 846.0 0 313 225 510 846.0 0 510 226 93 846.0 0 93 227 75 846.0 0 75 228 113 846.0 0 113 229 69 846.0 0 69 230 83 846.0 0 83 231 195 846.0 0 195 232 317 846.0 0 317 233 739 846.0 0 739 234 870 846.0 0 870 235 1302 846.0 0 1302 236 1639 846.0 0 1639 237 1673 846.0 0 1673 238 16823 846.0 0 16823 239 130291 846.0 0 130291 240 5197 846.0 0 5197 241 254 846.0 0 254 242 301 846.0 0 301 243 244 846.0 0 244 244 298 846.0 0 298 245 393 846.0 0 393 246 493 846.0 0 493 247 1371 846.0 0 1371 248 30311 846.0 0 30311 249 11020 846.0 0 11020 250 4981 846.0 0 4981 251 2937 846.0 0 2937 252 45 846.0 0 45 253 54 846.0 0 54 254 51 846.0 0 51 255 42 846.0 0 42 256 27 846.0 0 27 257 19 846.0 0 19 258 62 846.0 0 62 259 57 846.0 0 57 260 15 846.0 0 15 261 70 846.0 0 70 262 6 846.0 0 6 263 8 846.0 0 8 264 79 846.0 0 79 265 41 846.0 0 41 266 21 846.0 0 21 267 21 846.0 0 21 268 19 846.0 0 19 269 27 846.0 0 27 270 10 846.0 0 10 271 31 846.0 0 31 272 30 846.0 0 30 273 8 846.0 0 8 274 22 846.0 0 22 275 19 846.0 0 19 276 10 846.0 0 10 277 22 846.0 0 22 278 35 846.0 0 35 279 65 846.0 0 65 280 108 846.0 0 108 281 122 846.0 0 122 282 421 846.0 0 421 283 270 846.0 0 270 284 941 846.0 0 941 285 71 846.0 0 71 286 44 846.0 0 44 287 42 846.0 0 42 288 22 846.0 0 22 289 15 846.0 0 15 290 10 846.0 0 10 291 15 846.0 0 15 292 21 846.0 0 21 293 27 846.0 0 27 294 33 846.0 0 33 295 124 846.0 0 124 296 114 846.0 0 114 297 83 846.0 0 83 298 38 846.0 0 38 299 131 846.0 0 131 300 31 846.0 0 31 301 70 846.0 0 70 Finished in 1.30 s (42 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 30,591 Reads with adapters: 7,805 (25.5%) Reads that were too short: 57 (0.2%) Reads written (passing filters): 7,748 (25.3%) Total basepairs processed: 7,190,645 bp Total written (filtered): 2,103,701 bp (29.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7805 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 16 53 0.0 3 0 50 2 1 17 7377 0.0 3 7223 138 15 1 18 20 0.0 3 2 17 0 1 19 7 0.0 3 1 0 2 4 20 10 0.0 3 0 0 0 10 26 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 70 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 75 26 0.0 3 0 0 0 26 80 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 91 15 0.0 3 0 0 0 15 92 36 0.0 3 0 0 0 36 93 3 0.0 3 0 0 0 3 94 3 0.0 3 0 0 0 3 95 1 0.0 3 0 0 0 1 96 23 0.0 3 0 0 0 23 98 37 0.0 3 0 0 0 37 99 4 0.0 3 0 0 0 4 100 5 0.0 3 0 0 0 5 101 5 0.0 3 0 0 0 5 108 2 0.0 3 0 0 0 2 109 13 0.0 3 0 0 0 13 112 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 116 60 0.0 3 0 0 0 60 117 2 0.0 3 0 0 0 2 118 6 0.0 3 0 0 0 6 119 21 0.0 3 0 0 0 21 120 2 0.0 3 0 0 0 2 124 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 160 3 0.0 3 0 0 0 3 161 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 180 2 0.0 3 0 0 0 2 181 2 0.0 3 0 0 0 2 182 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 210 3 0.0 3 0 0 0 3 211 5 0.0 3 0 0 2 3 215 1 0.0 3 0 0 0 1 216 3 0.0 3 0 0 0 3 217 1 0.0 3 0 0 0 1 218 12 0.0 3 0 0 0 12 219 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.28 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 7,748 Reads with adapters: 587 (7.6%) Reads written (passing filters): 7,748 (100.0%) Total basepairs processed: 2,103,701 bp Total written (filtered): 2,101,936 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 587 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.8% C: 69.2% G: 7.8% T: 2.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 584 121.1 0 584 4 2 30.3 0 2 5 1 7.6 0 1