This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 92.50 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 569,285 (16.4%) Reads that were too short: 211,924 (6.1%) Reads written (passing filters): 357,361 (10.3%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 62,149,400 bp (6.0%) === Adapter 1 === Sequence: AAGACA; Type: regular 5'; Length: 6; Trimmed: 569285 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 205 846.0 0 205 7 289 846.0 0 289 8 364 846.0 0 364 9 22379 846.0 0 22379 10 164 846.0 0 164 11 35 846.0 0 35 12 18 846.0 0 18 13 12 846.0 0 12 14 20 846.0 0 20 15 11 846.0 0 11 16 22 846.0 0 22 17 13 846.0 0 13 18 8 846.0 0 8 19 6 846.0 0 6 20 13 846.0 0 13 21 12 846.0 0 12 22 15 846.0 0 15 23 16 846.0 0 16 24 24 846.0 0 24 25 21 846.0 0 21 26 20 846.0 0 20 27 39 846.0 0 39 28 23 846.0 0 23 29 35 846.0 0 35 30 27 846.0 0 27 31 36 846.0 0 36 32 53 846.0 0 53 33 102 846.0 0 102 34 64 846.0 0 64 35 88 846.0 0 88 36 53 846.0 0 53 37 71 846.0 0 71 38 88 846.0 0 88 39 6187 846.0 0 6187 40 67 846.0 0 67 41 25 846.0 0 25 42 17 846.0 0 17 43 13 846.0 0 13 44 11 846.0 0 11 45 10 846.0 0 10 46 11 846.0 0 11 47 25 846.0 0 25 48 16 846.0 0 16 49 17 846.0 0 17 50 15 846.0 0 15 51 14 846.0 0 14 52 15 846.0 0 15 53 14 846.0 0 14 54 39 846.0 0 39 55 18 846.0 0 18 56 21 846.0 0 21 57 7 846.0 0 7 58 21 846.0 0 21 59 75 846.0 0 75 60 21 846.0 0 21 61 26 846.0 0 26 62 39 846.0 0 39 63 733 846.0 0 733 64 146 846.0 0 146 65 49 846.0 0 49 66 30 846.0 0 30 67 31 846.0 0 31 68 249 846.0 0 249 69 49 846.0 0 49 70 197 846.0 0 197 71 1103 846.0 0 1103 72 1388 846.0 0 1388 73 3322 846.0 0 3322 74 3396 846.0 0 3396 75 2009 846.0 0 2009 76 1247 846.0 0 1247 77 1557 846.0 0 1557 78 2911 846.0 0 2911 79 2899 846.0 0 2899 80 2917 846.0 0 2917 81 3511 846.0 0 3511 82 2682 846.0 0 2682 83 3202 846.0 0 3202 84 3474 846.0 0 3474 85 4189 846.0 0 4189 86 3112 846.0 0 3112 87 4576 846.0 0 4576 88 3606 846.0 0 3606 89 2065 846.0 0 2065 90 1532 846.0 0 1532 91 3270 846.0 0 3270 92 3023 846.0 0 3023 93 1653 846.0 0 1653 94 2474 846.0 0 2474 95 1643 846.0 0 1643 96 1292 846.0 0 1292 97 962 846.0 0 962 98 966 846.0 0 966 99 915 846.0 0 915 100 2543 846.0 0 2543 101 2754 846.0 0 2754 102 1831 846.0 0 1831 103 2493 846.0 0 2493 104 1958 846.0 0 1958 105 1366 846.0 0 1366 106 804 846.0 0 804 107 909 846.0 0 909 108 1722 846.0 0 1722 109 2409 846.0 0 2409 110 2780 846.0 0 2780 111 2267 846.0 0 2267 112 2611 846.0 0 2611 113 2019 846.0 0 2019 114 1909 846.0 0 1909 115 1669 846.0 0 1669 116 2765 846.0 0 2765 117 2938 846.0 0 2938 118 3107 846.0 0 3107 119 2952 846.0 0 2952 120 3090 846.0 0 3090 121 2628 846.0 0 2628 122 2875 846.0 0 2875 123 2475 846.0 0 2475 124 2291 846.0 0 2291 125 2419 846.0 0 2419 126 2074 846.0 0 2074 127 1732 846.0 0 1732 128 1795 846.0 0 1795 129 2345 846.0 0 2345 130 2085 846.0 0 2085 131 3063 846.0 0 3063 132 2300 846.0 0 2300 133 1989 846.0 0 1989 134 2108 846.0 0 2108 135 2087 846.0 0 2087 136 1978 846.0 0 1978 137 2288 846.0 0 2288 138 2581 846.0 0 2581 139 2462 846.0 0 2462 140 2049 846.0 0 2049 141 2324 846.0 0 2324 142 2826 846.0 0 2826 143 2646 846.0 0 2646 144 2310 846.0 0 2310 145 2850 846.0 0 2850 146 2617 846.0 0 2617 147 2501 846.0 0 2501 148 2319 846.0 0 2319 149 1933 846.0 0 1933 150 2379 846.0 0 2379 151 2097 846.0 0 2097 152 2881 846.0 0 2881 153 2644 846.0 0 2644 154 1956 846.0 0 1956 155 2066 846.0 0 2066 156 1851 846.0 0 1851 157 2083 846.0 0 2083 158 2189 846.0 0 2189 159 1891 846.0 0 1891 160 2069 846.0 0 2069 161 1880 846.0 0 1880 162 2064 846.0 0 2064 163 1844 846.0 0 1844 164 1766 846.0 0 1766 165 1503 846.0 0 1503 166 3680 846.0 0 3680 167 6390 846.0 0 6390 168 2798 846.0 0 2798 169 2315 846.0 0 2315 170 2875 846.0 0 2875 171 3391 846.0 0 3391 172 2921 846.0 0 2921 173 1953 846.0 0 1953 174 1832 846.0 0 1832 175 2304 846.0 0 2304 176 1971 846.0 0 1971 177 2312 846.0 0 2312 178 2233 846.0 0 2233 179 2133 846.0 0 2133 180 2049 846.0 0 2049 181 2474 846.0 0 2474 182 7838 846.0 0 7838 183 16898 846.0 0 16898 184 2892 846.0 0 2892 185 2449 846.0 0 2449 186 1786 846.0 0 1786 187 2051 846.0 0 2051 188 2066 846.0 0 2066 189 1968 846.0 0 1968 190 2069 846.0 0 2069 191 1947 846.0 0 1947 192 1795 846.0 0 1795 193 1863 846.0 0 1863 194 1473 846.0 0 1473 195 1952 846.0 0 1952 196 1492 846.0 0 1492 197 2392 846.0 0 2392 198 3166 846.0 0 3166 199 2185 846.0 0 2185 200 2421 846.0 0 2421 201 1568 846.0 0 1568 202 1663 846.0 0 1663 203 2078 846.0 0 2078 204 2073 846.0 0 2073 205 2055 846.0 0 2055 206 1823 846.0 0 1823 207 1661 846.0 0 1661 208 1516 846.0 0 1516 209 1427 846.0 0 1427 210 1509 846.0 0 1509 211 1777 846.0 0 1777 212 1727 846.0 0 1727 213 2694 846.0 0 2694 214 2612 846.0 0 2612 215 2681 846.0 0 2681 216 1696 846.0 0 1696 217 1577 846.0 0 1577 218 2044 846.0 0 2044 219 2339 846.0 0 2339 220 1576 846.0 0 1576 221 1406 846.0 0 1406 222 1511 846.0 0 1511 223 1828 846.0 0 1828 224 1646 846.0 0 1646 225 1448 846.0 0 1448 226 1389 846.0 0 1389 227 1628 846.0 0 1628 228 1875 846.0 0 1875 229 1719 846.0 0 1719 230 1473 846.0 0 1473 231 1451 846.0 0 1451 232 1547 846.0 0 1547 233 1788 846.0 0 1788 234 1746 846.0 0 1746 235 1591 846.0 0 1591 236 1158 846.0 0 1158 237 1585 846.0 0 1585 238 1857 846.0 0 1857 239 2342 846.0 0 2342 240 1961 846.0 0 1961 241 1675 846.0 0 1675 242 2165 846.0 0 2165 243 1709 846.0 0 1709 244 1616 846.0 0 1616 245 1371 846.0 0 1371 246 1896 846.0 0 1896 247 1726 846.0 0 1726 248 1796 846.0 0 1796 249 1692 846.0 0 1692 250 2068 846.0 0 2068 251 2014 846.0 0 2014 252 2532 846.0 0 2532 253 1923 846.0 0 1923 254 1465 846.0 0 1465 255 1192 846.0 0 1192 256 1238 846.0 0 1238 257 1320 846.0 0 1320 258 1265 846.0 0 1265 259 1226 846.0 0 1226 260 1466 846.0 0 1466 261 2157 846.0 0 2157 262 3316 846.0 0 3316 263 3305 846.0 0 3305 264 2464 846.0 0 2464 265 1820 846.0 0 1820 266 1929 846.0 0 1929 267 10656 846.0 0 10656 268 6223 846.0 0 6223 269 5864 846.0 0 5864 270 2430 846.0 0 2430 271 1584 846.0 0 1584 272 2181 846.0 0 2181 273 2145 846.0 0 2145 274 1325 846.0 0 1325 275 1275 846.0 0 1275 276 1293 846.0 0 1293 277 1912 846.0 0 1912 278 2640 846.0 0 2640 279 2126 846.0 0 2126 280 2165 846.0 0 2165 281 2178 846.0 0 2178 282 2827 846.0 0 2827 283 1666 846.0 0 1666 284 1773 846.0 0 1773 285 1578 846.0 0 1578 286 1713 846.0 0 1713 287 1595 846.0 0 1595 288 1345 846.0 0 1345 289 1637 846.0 0 1637 290 2371 846.0 0 2371 291 2224 846.0 0 2224 292 2359 846.0 0 2359 293 1827 846.0 0 1827 294 1519 846.0 0 1519 295 1583 846.0 0 1583 296 1955 846.0 0 1955 297 2341 846.0 0 2341 298 2629 846.0 0 2629 299 7207 846.0 0 7207 300 3217 846.0 0 3217 301 4738 846.0 0 4738 Finished in 11.46 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 357,361 Reads with adapters: 23,429 (6.6%) Reads that were too short: 121 (0.0%) Reads written (passing filters): 23,308 (6.5%) Total basepairs processed: 62,149,400 bp Total written (filtered): 6,382,892 bp (10.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 23429 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 4 0.0 3 0 0 4 16 194 0.0 3 0 182 6 6 17 22835 0.0 3 22240 526 57 12 18 45 0.0 3 1 38 0 6 19 13 0.0 3 1 1 3 8 20 37 0.0 3 0 0 0 37 21 27 0.0 3 0 0 1 26 22 1 0.0 3 0 0 0 1 23 3 0.0 3 0 0 0 3 24 20 0.0 3 0 0 1 19 25 1 0.0 3 0 0 0 1 27 3 0.0 3 1 0 0 2 28 2 0.0 3 0 0 0 2 30 2 0.0 3 0 0 0 2 32 2 0.0 3 0 0 0 2 33 3 0.0 3 0 0 0 3 34 3 0.0 3 0 0 0 3 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 39 2 0.0 3 0 0 0 2 40 3 0.0 3 0 0 0 3 41 1 0.0 3 0 0 0 1 42 4 0.0 3 0 0 0 4 43 3 0.0 3 0 0 0 3 45 1 0.0 3 0 0 0 1 46 3 0.0 3 0 0 0 3 47 3 0.0 3 0 0 0 3 48 3 0.0 3 0 0 0 3 49 4 0.0 3 0 0 0 4 50 2 0.0 3 0 0 0 2 51 6 0.0 3 0 0 0 6 52 4 0.0 3 0 0 0 4 53 2 0.0 3 0 0 0 2 54 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 56 2 0.0 3 0 0 0 2 57 3 0.0 3 0 0 0 3 60 4 0.0 3 0 0 0 4 61 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 65 2 0.0 3 0 0 1 1 66 4 0.0 3 0 0 0 4 67 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 70 1 0.0 3 0 0 0 1 71 5 0.0 3 0 0 0 5 72 2 0.0 3 0 0 0 2 73 1 0.0 3 0 0 0 1 74 5 0.0 3 0 0 0 5 75 1 0.0 3 0 0 1 76 2 0.0 3 0 0 0 2 77 4 0.0 3 0 0 0 4 78 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 82 3 0.0 3 0 0 0 3 83 3 0.0 3 0 0 0 3 85 5 0.0 3 0 0 0 5 86 2 0.0 3 0 0 0 2 87 3 0.0 3 0 0 0 3 88 4 0.0 3 0 0 0 4 89 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 92 2 0.0 3 0 0 0 2 93 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 3 0.0 3 0 0 0 3 101 3 0.0 3 0 0 0 3 102 4 0.0 3 0 0 0 4 103 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 105 2 0.0 3 0 0 0 2 106 2 0.0 3 0 0 0 2 107 2 0.0 3 0 0 1 1 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 114 2 0.0 3 0 0 0 2 118 3 0.0 3 0 0 0 3 119 2 0.0 3 0 0 0 2 120 2 0.0 3 0 0 0 2 121 2 0.0 3 0 0 0 2 122 1 0.0 3 0 0 0 1 123 3 0.0 3 0 0 0 3 124 1 0.0 3 0 0 0 1 125 4 0.0 3 0 0 0 4 126 4 0.0 3 0 0 0 4 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 3 0.0 3 0 0 0 3 131 2 0.0 3 0 0 0 2 133 2 0.0 3 0 0 0 2 135 2 0.0 3 0 0 0 2 136 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 4 0.0 3 0 0 0 4 150 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 160 2 0.0 3 0 0 0 2 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 2 0.0 3 0 0 0 2 171 1 0.0 3 0 0 0 1 174 2 0.0 3 0 0 1 1 175 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 217 2 0.0 3 0 0 0 2 223 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 229 2 0.0 3 0 0 0 2 230 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.83 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 23,308 Reads with adapters: 1,373 (5.9%) Reads written (passing filters): 23,308 (100.0%) Total basepairs processed: 6,382,892 bp Total written (filtered): 6,378,753 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1373 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 36.6% C: 5.0% G: 54.3% T: 4.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1353 364.2 0 1353 4 20 91.0 0 20