This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGTGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 90.65 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 71,510 (2.1%) Reads that were too short: 8,475 (0.2%) Reads written (passing filters): 63,035 (1.8%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 14,756,261 bp (1.4%) === Adapter 1 === Sequence: AGTGTT; Type: regular 5'; Length: 6; Trimmed: 71510 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 186 846.0 0 186 7 351 846.0 0 351 8 25321 846.0 0 25321 9 75 846.0 0 75 10 46 846.0 0 46 11 49 846.0 0 49 12 46 846.0 0 46 13 40 846.0 0 40 14 32 846.0 0 32 15 50 846.0 0 50 16 44 846.0 0 44 17 54 846.0 0 54 18 53 846.0 0 53 19 40 846.0 0 40 20 33 846.0 0 33 21 65 846.0 0 65 22 76 846.0 0 76 23 52 846.0 0 52 24 55 846.0 0 55 25 51 846.0 0 51 26 45 846.0 0 45 27 69 846.0 0 69 28 56 846.0 0 56 29 45 846.0 0 45 30 79 846.0 0 79 31 66 846.0 0 66 32 56 846.0 0 56 33 74 846.0 0 74 34 62 846.0 0 62 35 50 846.0 0 50 36 39 846.0 0 39 37 44 846.0 0 44 38 47 846.0 0 47 39 38 846.0 0 38 40 47 846.0 0 47 41 69 846.0 0 69 42 47 846.0 0 47 43 35 846.0 0 35 44 55 846.0 0 55 45 49 846.0 0 49 46 48 846.0 0 48 47 42 846.0 0 42 48 58 846.0 0 58 49 51 846.0 0 51 50 55 846.0 0 55 51 70 846.0 0 70 52 68 846.0 0 68 53 53 846.0 0 53 54 58 846.0 0 58 55 51 846.0 0 51 56 77 846.0 0 77 57 61 846.0 0 61 58 52 846.0 0 52 59 63 846.0 0 63 60 55 846.0 0 55 61 115 846.0 0 115 62 176 846.0 0 176 63 168 846.0 0 168 64 232 846.0 0 232 65 109 846.0 0 109 66 120 846.0 0 120 67 184 846.0 0 184 68 5150 846.0 0 5150 69 876 846.0 0 876 70 2718 846.0 0 2718 71 72 846.0 0 72 72 58 846.0 0 58 73 128 846.0 0 128 74 52 846.0 0 52 75 92 846.0 0 92 76 42 846.0 0 42 77 54 846.0 0 54 78 57 846.0 0 57 79 109 846.0 0 109 80 67 846.0 0 67 81 72 846.0 0 72 82 69 846.0 0 69 83 40 846.0 0 40 84 46 846.0 0 46 85 39 846.0 0 39 86 45 846.0 0 45 87 53 846.0 0 53 88 51 846.0 0 51 89 56 846.0 0 56 90 49 846.0 0 49 91 48 846.0 0 48 92 63 846.0 0 63 93 95 846.0 0 95 94 246 846.0 0 246 95 216 846.0 0 216 96 159 846.0 0 159 97 81 846.0 0 81 98 126 846.0 0 126 99 1390 846.0 0 1390 100 3897 846.0 0 3897 101 2715 846.0 0 2715 102 59 846.0 0 59 103 43 846.0 0 43 104 45 846.0 0 45 105 46 846.0 0 46 106 41 846.0 0 41 107 43 846.0 0 43 108 53 846.0 0 53 109 47 846.0 0 47 110 66 846.0 0 66 111 54 846.0 0 54 112 66 846.0 0 66 113 55 846.0 0 55 114 54 846.0 0 54 115 62 846.0 0 62 116 48 846.0 0 48 117 63 846.0 0 63 118 65 846.0 0 65 119 48 846.0 0 48 120 62 846.0 0 62 121 52 846.0 0 52 122 46 846.0 0 46 123 63 846.0 0 63 124 65 846.0 0 65 125 65 846.0 0 65 126 83 846.0 0 83 127 97 846.0 0 97 128 213 846.0 0 213 129 106 846.0 0 106 130 86 846.0 0 86 131 121 846.0 0 121 132 133 846.0 0 133 133 121 846.0 0 121 134 77 846.0 0 77 135 54 846.0 0 54 136 92 846.0 0 92 137 72 846.0 0 72 138 59 846.0 0 59 139 69 846.0 0 69 140 71 846.0 0 71 141 87 846.0 0 87 142 242 846.0 0 242 143 265 846.0 0 265 144 201 846.0 0 201 145 4104 846.0 0 4104 146 374 846.0 0 374 147 340 846.0 0 340 148 49 846.0 0 49 149 79 846.0 0 79 150 41 846.0 0 41 151 33 846.0 0 33 152 40 846.0 0 40 153 43 846.0 0 43 154 56 846.0 0 56 155 44 846.0 0 44 156 57 846.0 0 57 157 45 846.0 0 45 158 48 846.0 0 48 159 50 846.0 0 50 160 41 846.0 0 41 161 66 846.0 0 66 162 46 846.0 0 46 163 52 846.0 0 52 164 57 846.0 0 57 165 51 846.0 0 51 166 55 846.0 0 55 167 136 846.0 0 136 168 105 846.0 0 105 169 84 846.0 0 84 170 48 846.0 0 48 171 44 846.0 0 44 172 51 846.0 0 51 173 71 846.0 0 71 174 67 846.0 0 67 175 56 846.0 0 56 176 83 846.0 0 83 177 50 846.0 0 50 178 57 846.0 0 57 179 48 846.0 0 48 180 49 846.0 0 49 181 51 846.0 0 51 182 66 846.0 0 66 183 62 846.0 0 62 184 48 846.0 0 48 185 76 846.0 0 76 186 54 846.0 0 54 187 50 846.0 0 50 188 64 846.0 0 64 189 54 846.0 0 54 190 74 846.0 0 74 191 166 846.0 0 166 192 166 846.0 0 166 193 398 846.0 0 398 194 389 846.0 0 389 195 156 846.0 0 156 196 85 846.0 0 85 197 171 846.0 0 171 198 386 846.0 0 386 199 587 846.0 0 587 200 687 846.0 0 687 201 177 846.0 0 177 202 74 846.0 0 74 203 91 846.0 0 91 204 72 846.0 0 72 205 94 846.0 0 94 206 106 846.0 0 106 207 122 846.0 0 122 208 68 846.0 0 68 209 58 846.0 0 58 210 66 846.0 0 66 211 158 846.0 0 158 212 125 846.0 0 125 213 117 846.0 0 117 214 84 846.0 0 84 215 71 846.0 0 71 216 168 846.0 0 168 217 63 846.0 0 63 218 62 846.0 0 62 219 177 846.0 0 177 220 105 846.0 0 105 221 119 846.0 0 119 222 100 846.0 0 100 223 78 846.0 0 78 224 86 846.0 0 86 225 75 846.0 0 75 226 74 846.0 0 74 227 62 846.0 0 62 228 65 846.0 0 65 229 53 846.0 0 53 230 69 846.0 0 69 231 85 846.0 0 85 232 227 846.0 0 227 233 252 846.0 0 252 234 277 846.0 0 277 235 96 846.0 0 96 236 107 846.0 0 107 237 110 846.0 0 110 238 97 846.0 0 97 239 98 846.0 0 98 240 126 846.0 0 126 241 90 846.0 0 90 242 113 846.0 0 113 243 90 846.0 0 90 244 67 846.0 0 67 245 47 846.0 0 47 246 67 846.0 0 67 247 80 846.0 0 80 248 46 846.0 0 46 249 57 846.0 0 57 250 84 846.0 0 84 251 58 846.0 0 58 252 80 846.0 0 80 253 116 846.0 0 116 254 87 846.0 0 87 255 86 846.0 0 86 256 94 846.0 0 94 257 99 846.0 0 99 258 83 846.0 0 83 259 66 846.0 0 66 260 71 846.0 0 71 261 80 846.0 0 80 262 75 846.0 0 75 263 103 846.0 0 103 264 64 846.0 0 64 265 81 846.0 0 81 266 53 846.0 0 53 267 66 846.0 0 66 268 77 846.0 0 77 269 76 846.0 0 76 270 46 846.0 0 46 271 46 846.0 0 46 272 68 846.0 0 68 273 78 846.0 0 78 274 66 846.0 0 66 275 72 846.0 0 72 276 56 846.0 0 56 277 42 846.0 0 42 278 57 846.0 0 57 279 61 846.0 0 61 280 54 846.0 0 54 281 62 846.0 0 62 282 77 846.0 0 77 283 82 846.0 0 82 284 119 846.0 0 119 285 49 846.0 0 49 286 56 846.0 0 56 287 41 846.0 0 41 288 52 846.0 0 52 289 47 846.0 0 47 290 37 846.0 0 37 291 75 846.0 0 75 292 90 846.0 0 90 293 78 846.0 0 78 294 32 846.0 0 32 295 48 846.0 0 48 296 51 846.0 0 51 297 59 846.0 0 59 298 67 846.0 0 67 299 51 846.0 0 51 300 165 846.0 0 165 301 98 846.0 0 98 Finished in 2.86 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 63,035 Reads with adapters: 25,653 (40.7%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 25,649 (40.7%) Total basepairs processed: 14,756,261 bp Total written (filtered): 7,001,008 bp (47.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 25653 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 15 0.0 3 0 0 14 1 19 199 0.0 3 0 194 4 0 1 20 25308 0.0 4 25029 275 4 21 90 0.0 4 11 78 1 22 5 0.0 4 1 0 3 0 1 23 13 0.0 4 0 0 0 12 1 24 1 0.0 4 0 0 0 0 1 39 1 0.0 4 1 79 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 111 15 0.0 4 0 0 0 14 1 193 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.88 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 25,649 Reads with adapters: 979 (3.8%) Reads written (passing filters): 25,649 (100.0%) Total basepairs processed: 7,001,008 bp Total written (filtered): 6,980,289 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 979 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.9% C: 5.0% G: 93.4% T: 0.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 5 400.8 0 5 4 1 100.2 0 1 9 1 0.1 0 0 1 10 72 0.0 1 1 71 11 852 0.0 1 1 851 12 1 0.0 1 1 14 1 0.0 1 0 1 151 1 0.0 1 0 1 190 1 0.0 1 1 209 1 0.0 1 0 1 224 33 0.0 1 30 3 225 1 0.0 1 1 257 1 0.0 1 1 263 2 0.0 1 2 268 1 0.0 1 1 270 1 0.0 1 1 271 3 0.0 1 3 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.