This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 88.02 s (25 us/read; 2.36 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 30,747 (0.9%) Reads that were too short: 8,238 (0.2%) Reads written (passing filters): 22,509 (0.6%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 4,222,475 bp (0.4%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 30747 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 91 846.0 0 91 7 261 846.0 0 261 8 3556 846.0 0 3556 9 67 846.0 0 67 10 25 846.0 0 25 11 44 846.0 0 44 12 34 846.0 0 34 13 37 846.0 0 37 14 46 846.0 0 46 15 37 846.0 0 37 16 29 846.0 0 29 17 26 846.0 0 26 18 30 846.0 0 30 19 27 846.0 0 27 20 29 846.0 0 29 21 21 846.0 0 21 22 20 846.0 0 20 23 29 846.0 0 29 24 31 846.0 0 31 25 22 846.0 0 22 26 26 846.0 0 26 27 31 846.0 0 31 28 26 846.0 0 26 29 40 846.0 0 40 30 22 846.0 0 22 31 34 846.0 0 34 32 12 846.0 0 12 33 26 846.0 0 26 34 22 846.0 0 22 35 35 846.0 0 35 36 36 846.0 0 36 37 62 846.0 0 62 38 40 846.0 0 40 39 31 846.0 0 31 40 45 846.0 0 45 41 34 846.0 0 34 42 32 846.0 0 32 43 31 846.0 0 31 44 32 846.0 0 32 45 27 846.0 0 27 46 39 846.0 0 39 47 30 846.0 0 30 48 23 846.0 0 23 49 41 846.0 0 41 50 33 846.0 0 33 51 29 846.0 0 29 52 63 846.0 0 63 53 68 846.0 0 68 54 53 846.0 0 53 55 37 846.0 0 37 56 29 846.0 0 29 57 22 846.0 0 22 58 40 846.0 0 40 59 33 846.0 0 33 60 40 846.0 0 40 61 43 846.0 0 43 62 35 846.0 0 35 63 54 846.0 0 54 64 107 846.0 0 107 65 26 846.0 0 26 66 33 846.0 0 33 67 41 846.0 0 41 68 46 846.0 0 46 69 28 846.0 0 28 70 27 846.0 0 27 71 43 846.0 0 43 72 124 846.0 0 124 73 732 846.0 0 732 74 527 846.0 0 527 75 475 846.0 0 475 76 23 846.0 0 23 77 16 846.0 0 16 78 26 846.0 0 26 79 37 846.0 0 37 80 28 846.0 0 28 81 28 846.0 0 28 82 37 846.0 0 37 83 30 846.0 0 30 84 70 846.0 0 70 85 84 846.0 0 84 86 129 846.0 0 129 87 103 846.0 0 103 88 213 846.0 0 213 89 64 846.0 0 64 90 38 846.0 0 38 91 44 846.0 0 44 92 30 846.0 0 30 93 28 846.0 0 28 94 43 846.0 0 43 95 49 846.0 0 49 96 20 846.0 0 20 97 33 846.0 0 33 98 33 846.0 0 33 99 21 846.0 0 21 100 33 846.0 0 33 101 34 846.0 0 34 102 35 846.0 0 35 103 21 846.0 0 21 104 28 846.0 0 28 105 44 846.0 0 44 106 23 846.0 0 23 107 23 846.0 0 23 108 39 846.0 0 39 109 32 846.0 0 32 110 45 846.0 0 45 111 28 846.0 0 28 112 21 846.0 0 21 113 32 846.0 0 32 114 16 846.0 0 16 115 49 846.0 0 49 116 30 846.0 0 30 117 31 846.0 0 31 118 41 846.0 0 41 119 18 846.0 0 18 120 33 846.0 0 33 121 53 846.0 0 53 122 32 846.0 0 32 123 38 846.0 0 38 124 37 846.0 0 37 125 35 846.0 0 35 126 51 846.0 0 51 127 35 846.0 0 35 128 42 846.0 0 42 129 74 846.0 0 74 130 71 846.0 0 71 131 31 846.0 0 31 132 32 846.0 0 32 133 36 846.0 0 36 134 52 846.0 0 52 135 82 846.0 0 82 136 127 846.0 0 127 137 77 846.0 0 77 138 174 846.0 0 174 139 43 846.0 0 43 140 55 846.0 0 55 141 65 846.0 0 65 142 56 846.0 0 56 143 46 846.0 0 46 144 46 846.0 0 46 145 54 846.0 0 54 146 161 846.0 0 161 147 210 846.0 0 210 148 303 846.0 0 303 149 85 846.0 0 85 150 68 846.0 0 68 151 37 846.0 0 37 152 43 846.0 0 43 153 71 846.0 0 71 154 84 846.0 0 84 155 150 846.0 0 150 156 110 846.0 0 110 157 168 846.0 0 168 158 495 846.0 0 495 159 245 846.0 0 245 160 537 846.0 0 537 161 91 846.0 0 91 162 192 846.0 0 192 163 94 846.0 0 94 164 48 846.0 0 48 165 73 846.0 0 73 166 155 846.0 0 155 167 176 846.0 0 176 168 104 846.0 0 104 169 400 846.0 0 400 170 140 846.0 0 140 171 108 846.0 0 108 172 110 846.0 0 110 173 127 846.0 0 127 174 1150 846.0 0 1150 175 1912 846.0 0 1912 176 412 846.0 0 412 177 84 846.0 0 84 178 39 846.0 0 39 179 53 846.0 0 53 180 293 846.0 0 293 181 73 846.0 0 73 182 207 846.0 0 207 183 111 846.0 0 111 184 82 846.0 0 82 185 127 846.0 0 127 186 138 846.0 0 138 187 725 846.0 0 725 188 397 846.0 0 397 189 240 846.0 0 240 190 39 846.0 0 39 191 57 846.0 0 57 192 70 846.0 0 70 193 45 846.0 0 45 194 44 846.0 0 44 195 37 846.0 0 37 196 51 846.0 0 51 197 43 846.0 0 43 198 28 846.0 0 28 199 33 846.0 0 33 200 53 846.0 0 53 201 27 846.0 0 27 202 43 846.0 0 43 203 58 846.0 0 58 204 92 846.0 0 92 205 121 846.0 0 121 206 75 846.0 0 75 207 62 846.0 0 62 208 44 846.0 0 44 209 55 846.0 0 55 210 35 846.0 0 35 211 50 846.0 0 50 212 28 846.0 0 28 213 41 846.0 0 41 214 81 846.0 0 81 215 157 846.0 0 157 216 66 846.0 0 66 217 46 846.0 0 46 218 68 846.0 0 68 219 61 846.0 0 61 220 53 846.0 0 53 221 51 846.0 0 51 222 78 846.0 0 78 223 54 846.0 0 54 224 45 846.0 0 45 225 63 846.0 0 63 226 57 846.0 0 57 227 181 846.0 0 181 228 222 846.0 0 222 229 83 846.0 0 83 230 47 846.0 0 47 231 83 846.0 0 83 232 80 846.0 0 80 233 84 846.0 0 84 234 62 846.0 0 62 235 60 846.0 0 60 236 36 846.0 0 36 237 75 846.0 0 75 238 333 846.0 0 333 239 45 846.0 0 45 240 26 846.0 0 26 241 29 846.0 0 29 242 48 846.0 0 48 243 37 846.0 0 37 244 49 846.0 0 49 245 42 846.0 0 42 246 46 846.0 0 46 247 64 846.0 0 64 248 61 846.0 0 61 249 93 846.0 0 93 250 70 846.0 0 70 251 258 846.0 0 258 252 381 846.0 0 381 253 959 846.0 0 959 254 283 846.0 0 283 255 128 846.0 0 128 256 43 846.0 0 43 257 53 846.0 0 53 258 41 846.0 0 41 259 35 846.0 0 35 260 59 846.0 0 59 261 80 846.0 0 80 262 280 846.0 0 280 263 114 846.0 0 114 264 96 846.0 0 96 265 44 846.0 0 44 266 24 846.0 0 24 267 61 846.0 0 61 268 53 846.0 0 53 269 44 846.0 0 44 270 28 846.0 0 28 271 22 846.0 0 22 272 43 846.0 0 43 273 53 846.0 0 53 274 40 846.0 0 40 275 40 846.0 0 40 276 31 846.0 0 31 277 55 846.0 0 55 278 79 846.0 0 79 279 71 846.0 0 71 280 46 846.0 0 46 281 140 846.0 0 140 282 117 846.0 0 117 283 31 846.0 0 31 284 33 846.0 0 33 285 89 846.0 0 89 286 70 846.0 0 70 287 30 846.0 0 30 288 37 846.0 0 37 289 25 846.0 0 25 290 31 846.0 0 31 291 55 846.0 0 55 292 69 846.0 0 69 293 42 846.0 0 42 294 42 846.0 0 42 295 65 846.0 0 65 296 36 846.0 0 36 297 36 846.0 0 36 298 46 846.0 0 46 299 55 846.0 0 55 300 64 846.0 0 64 301 41 846.0 0 41 Finished in 0.83 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 22,509 Reads with adapters: 3,819 (17.0%) Reads that were too short: 44 (0.2%) Reads written (passing filters): 3,775 (16.8%) Total basepairs processed: 4,222,475 bp Total written (filtered): 1,039,673 bp (24.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3819 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 34 0.0 3 0 28 0 6 17 3532 0.0 3 3409 112 9 2 18 13 0.0 3 1 12 19 177 0.0 3 169 2 3 3 20 7 0.0 3 0 0 0 7 29 1 0.0 3 0 0 0 1 30 2 0.0 3 0 0 0 2 33 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 38 18 0.0 3 0 0 0 18 46 1 0.0 3 0 0 0 1 49 3 0.0 3 0 0 0 3 54 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 1 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 106 6 0.0 3 0 0 0 6 112 1 0.0 3 0 0 0 1 114 5 0.0 3 0 0 0 5 115 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.14 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 3,775 Reads with adapters: 66 (1.7%) Reads written (passing filters): 3,775 (100.0%) Total basepairs processed: 1,039,673 bp Total written (filtered): 1,039,475 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 66 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 72.7% C: 10.6% G: 12.1% T: 4.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 66 59.0 0 66