This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 91.40 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 179,532 (5.2%) Reads that were too short: 80,820 (2.3%) Reads written (passing filters): 98,712 (2.8%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 18,333,504 bp (1.8%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 179532 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 239 846.0 0 239 7 22761 846.0 0 22761 8 103 846.0 0 103 9 8 846.0 0 8 10 28 846.0 0 28 11 22 846.0 0 22 12 24 846.0 0 24 13 226 846.0 0 226 14 131 846.0 0 131 15 117 846.0 0 117 16 22 846.0 0 22 17 12 846.0 0 12 18 6 846.0 0 6 19 6 846.0 0 6 20 8 846.0 0 8 21 6 846.0 0 6 22 8 846.0 0 8 23 14 846.0 0 14 24 9 846.0 0 9 25 31 846.0 0 31 26 56 846.0 0 56 27 49 846.0 0 49 28 8 846.0 0 8 29 14 846.0 0 14 30 9 846.0 0 9 31 10 846.0 0 10 32 13 846.0 0 13 33 12 846.0 0 12 34 13 846.0 0 13 35 21 846.0 0 21 36 8 846.0 0 8 37 8 846.0 0 8 38 105 846.0 0 105 39 9 846.0 0 9 40 5 846.0 0 5 41 11 846.0 0 11 42 10 846.0 0 10 43 7 846.0 0 7 44 8 846.0 0 8 45 18 846.0 0 18 46 13 846.0 0 13 47 14 846.0 0 14 48 15 846.0 0 15 49 13 846.0 0 13 50 25 846.0 0 25 51 7 846.0 0 7 52 6 846.0 0 6 53 7 846.0 0 7 54 15 846.0 0 15 55 7 846.0 0 7 56 17 846.0 0 17 57 23 846.0 0 23 58 9 846.0 0 9 59 13 846.0 0 13 60 261 846.0 0 261 61 43 846.0 0 43 62 42 846.0 0 42 63 9 846.0 0 9 64 13 846.0 0 13 65 22 846.0 0 22 66 19 846.0 0 19 67 11 846.0 0 11 68 16 846.0 0 16 69 9 846.0 0 9 70 15 846.0 0 15 71 19 846.0 0 19 72 11 846.0 0 11 73 33 846.0 0 33 74 48 846.0 0 48 75 117 846.0 0 117 76 192 846.0 0 192 77 113 846.0 0 113 78 112 846.0 0 112 79 129 846.0 0 129 80 535 846.0 0 535 81 298 846.0 0 298 82 669 846.0 0 669 83 481 846.0 0 481 84 297 846.0 0 297 85 303 846.0 0 303 86 236 846.0 0 236 87 395 846.0 0 395 88 607 846.0 0 607 89 925 846.0 0 925 90 570 846.0 0 570 91 302 846.0 0 302 92 136 846.0 0 136 93 442 846.0 0 442 94 381 846.0 0 381 95 333 846.0 0 333 96 455 846.0 0 455 97 297 846.0 0 297 98 353 846.0 0 353 99 474 846.0 0 474 100 319 846.0 0 319 101 209 846.0 0 209 102 493 846.0 0 493 103 726 846.0 0 726 104 601 846.0 0 601 105 309 846.0 0 309 106 306 846.0 0 306 107 272 846.0 0 272 108 111 846.0 0 111 109 117 846.0 0 117 110 193 846.0 0 193 111 521 846.0 0 521 112 676 846.0 0 676 113 397 846.0 0 397 114 452 846.0 0 452 115 282 846.0 0 282 116 274 846.0 0 274 117 206 846.0 0 206 118 454 846.0 0 454 119 593 846.0 0 593 120 709 846.0 0 709 121 481 846.0 0 481 122 347 846.0 0 347 123 557 846.0 0 557 124 451 846.0 0 451 125 352 846.0 0 352 126 403 846.0 0 403 127 545 846.0 0 545 128 384 846.0 0 384 129 411 846.0 0 411 130 382 846.0 0 382 131 343 846.0 0 343 132 386 846.0 0 386 133 506 846.0 0 506 134 577 846.0 0 577 135 470 846.0 0 470 136 388 846.0 0 388 137 392 846.0 0 392 138 500 846.0 0 500 139 803 846.0 0 803 140 999 846.0 0 999 141 699 846.0 0 699 142 365 846.0 0 365 143 353 846.0 0 353 144 556 846.0 0 556 145 682 846.0 0 682 146 504 846.0 0 504 147 534 846.0 0 534 148 535 846.0 0 535 149 349 846.0 0 349 150 340 846.0 0 340 151 461 846.0 0 461 152 551 846.0 0 551 153 583 846.0 0 583 154 447 846.0 0 447 155 505 846.0 0 505 156 450 846.0 0 450 157 548 846.0 0 548 158 568 846.0 0 568 159 641 846.0 0 641 160 1736 846.0 0 1736 161 2196 846.0 0 2196 162 1017 846.0 0 1017 163 528 846.0 0 528 164 457 846.0 0 457 165 479 846.0 0 479 166 374 846.0 0 374 167 390 846.0 0 390 168 569 846.0 0 569 169 661 846.0 0 661 170 415 846.0 0 415 171 307 846.0 0 307 172 414 846.0 0 414 173 555 846.0 0 555 174 499 846.0 0 499 175 434 846.0 0 434 176 495 846.0 0 495 177 608 846.0 0 608 178 445 846.0 0 445 179 534 846.0 0 534 180 802 846.0 0 802 181 691 846.0 0 691 182 1000 846.0 0 1000 183 645 846.0 0 645 184 495 846.0 0 495 185 639 846.0 0 639 186 742 846.0 0 742 187 9327 846.0 0 9327 188 778 846.0 0 778 189 2985 846.0 0 2985 190 518 846.0 0 518 191 440 846.0 0 440 192 398 846.0 0 398 193 506 846.0 0 506 194 309 846.0 0 309 195 390 846.0 0 390 196 622 846.0 0 622 197 766 846.0 0 766 198 442 846.0 0 442 199 347 846.0 0 347 200 405 846.0 0 405 201 702 846.0 0 702 202 1301 846.0 0 1301 203 629 846.0 0 629 204 542 846.0 0 542 205 809 846.0 0 809 206 673 846.0 0 673 207 724 846.0 0 724 208 581 846.0 0 581 209 607 846.0 0 607 210 770 846.0 0 770 211 641 846.0 0 641 212 761 846.0 0 761 213 585 846.0 0 585 214 541 846.0 0 541 215 529 846.0 0 529 216 507 846.0 0 507 217 474 846.0 0 474 218 552 846.0 0 552 219 698 846.0 0 698 220 637 846.0 0 637 221 702 846.0 0 702 222 806 846.0 0 806 223 575 846.0 0 575 224 470 846.0 0 470 225 437 846.0 0 437 226 489 846.0 0 489 227 549 846.0 0 549 228 500 846.0 0 500 229 611 846.0 0 611 230 1229 846.0 0 1229 231 855 846.0 0 855 232 849 846.0 0 849 233 620 846.0 0 620 234 689 846.0 0 689 235 471 846.0 0 471 236 298 846.0 0 298 237 421 846.0 0 421 238 664 846.0 0 664 239 814 846.0 0 814 240 873 846.0 0 873 241 934 846.0 0 934 242 788 846.0 0 788 243 559 846.0 0 559 244 498 846.0 0 498 245 565 846.0 0 565 246 564 846.0 0 564 247 400 846.0 0 400 248 342 846.0 0 342 249 297 846.0 0 297 250 597 846.0 0 597 251 995 846.0 0 995 252 729 846.0 0 729 253 753 846.0 0 753 254 492 846.0 0 492 255 533 846.0 0 533 256 676 846.0 0 676 257 520 846.0 0 520 258 501 846.0 0 501 259 354 846.0 0 354 260 482 846.0 0 482 261 550 846.0 0 550 262 685 846.0 0 685 263 524 846.0 0 524 264 615 846.0 0 615 265 495 846.0 0 495 266 704 846.0 0 704 267 500 846.0 0 500 268 410 846.0 0 410 269 512 846.0 0 512 270 847 846.0 0 847 271 685 846.0 0 685 272 622 846.0 0 622 273 803 846.0 0 803 274 969 846.0 0 969 275 3885 846.0 0 3885 276 839 846.0 0 839 277 1607 846.0 0 1607 278 1506 846.0 0 1506 279 855 846.0 0 855 280 646 846.0 0 646 281 537 846.0 0 537 282 976 846.0 0 976 283 1138 846.0 0 1138 284 1409 846.0 0 1409 285 830 846.0 0 830 286 504 846.0 0 504 287 756 846.0 0 756 288 886 846.0 0 886 289 787 846.0 0 787 290 737 846.0 0 737 291 799 846.0 0 799 292 729 846.0 0 729 293 576 846.0 0 576 294 684 846.0 0 684 295 1058 846.0 0 1058 296 879 846.0 0 879 297 716 846.0 0 716 298 822 846.0 0 822 299 1062 846.0 0 1062 300 6589 846.0 0 6589 301 3025 846.0 0 3025 Finished in 3.58 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 98,712 Reads with adapters: 23,008 (23.3%) Reads that were too short: 69 (0.1%) Reads written (passing filters): 22,939 (23.2%) Total basepairs processed: 18,333,504 bp Total written (filtered): 6,347,673 bp (34.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 23008 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 6 0.0 3 0 0 6 16 197 0.0 3 0 172 19 6 17 22566 0.0 3 22019 478 55 14 18 67 0.0 3 6 55 2 4 19 23 0.0 3 1 0 13 9 20 39 0.0 3 0 0 0 39 24 2 0.0 3 0 0 0 2 25 1 0.0 3 0 0 0 1 27 1 0.0 3 1 32 2 0.0 3 0 0 0 2 33 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 37 2 0.0 3 0 0 0 2 38 2 0.0 3 0 0 0 2 40 2 0.0 3 0 0 0 2 46 2 0.0 3 0 0 0 2 49 2 0.0 3 0 0 1 1 50 2 0.0 3 0 0 0 2 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 3 0.0 3 0 0 0 3 64 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 70 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 3 0.0 3 0 0 0 3 77 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 90 7 0.0 3 0 0 0 7 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 96 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 107 2 0.0 3 0 0 0 2 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 123 5 0.0 3 0 0 0 5 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 132 2 0.0 3 0 0 0 2 135 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 141 13 0.0 3 0 0 0 13 158 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 175 4 0.0 3 0 0 0 4 200 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.81 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 22,939 Reads with adapters: 1,029 (4.5%) Reads written (passing filters): 22,939 (100.0%) Total basepairs processed: 6,347,673 bp Total written (filtered): 6,344,576 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1029 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 21.9% C: 63.8% G: 11.1% T: 3.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1022 358.4 0 1022 4 5 89.6 0 5 5 1 22.4 0 1 6 1 5.6 0 1