This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AACCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 89.70 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 101,759 (2.9%) Reads that were too short: 43,295 (1.2%) Reads written (passing filters): 58,464 (1.7%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 12,349,847 bp (1.2%) === Adapter 1 === Sequence: AACCGA; Type: regular 5'; Length: 6; Trimmed: 101759 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 206 846.0 0 206 7 195 846.0 0 195 8 679 846.0 0 679 9 22231 846.0 0 22231 10 42 846.0 0 42 11 15 846.0 0 15 12 23 846.0 0 23 13 18 846.0 0 18 14 7 846.0 0 7 15 12 846.0 0 12 16 16 846.0 0 16 17 20 846.0 0 20 18 14 846.0 0 14 19 9 846.0 0 9 20 23 846.0 0 23 21 15 846.0 0 15 22 14 846.0 0 14 23 12 846.0 0 12 24 25 846.0 0 25 25 17 846.0 0 17 26 16 846.0 0 16 27 10 846.0 0 10 28 15 846.0 0 15 29 11 846.0 0 11 30 16 846.0 0 16 31 10 846.0 0 10 32 17 846.0 0 17 33 24 846.0 0 24 34 13 846.0 0 13 35 10 846.0 0 10 36 17 846.0 0 17 37 11 846.0 0 11 38 22 846.0 0 22 39 15 846.0 0 15 40 16 846.0 0 16 41 23 846.0 0 23 42 19 846.0 0 19 43 18 846.0 0 18 44 9 846.0 0 9 45 19 846.0 0 19 46 15 846.0 0 15 47 16 846.0 0 16 48 25 846.0 0 25 49 21 846.0 0 21 50 20 846.0 0 20 51 27 846.0 0 27 52 21 846.0 0 21 53 14 846.0 0 14 54 7 846.0 0 7 55 14 846.0 0 14 56 11 846.0 0 11 57 20 846.0 0 20 58 19 846.0 0 19 59 10 846.0 0 10 60 16 846.0 0 16 61 28 846.0 0 28 62 28 846.0 0 28 63 14 846.0 0 14 64 18 846.0 0 18 65 15 846.0 0 15 66 8 846.0 0 8 67 22 846.0 0 22 68 17 846.0 0 17 69 15 846.0 0 15 70 18 846.0 0 18 71 24 846.0 0 24 72 23 846.0 0 23 73 22 846.0 0 22 74 10 846.0 0 10 75 13 846.0 0 13 76 15 846.0 0 15 77 26 846.0 0 26 78 45 846.0 0 45 79 72 846.0 0 72 80 76 846.0 0 76 81 117 846.0 0 117 82 146 846.0 0 146 83 466 846.0 0 466 84 787 846.0 0 787 85 325 846.0 0 325 86 81 846.0 0 81 87 59 846.0 0 59 88 37 846.0 0 37 89 60 846.0 0 60 90 150 846.0 0 150 91 107 846.0 0 107 92 242 846.0 0 242 93 382 846.0 0 382 94 345 846.0 0 345 95 469 846.0 0 469 96 182 846.0 0 182 97 111 846.0 0 111 98 96 846.0 0 96 99 118 846.0 0 118 100 189 846.0 0 189 101 225 846.0 0 225 102 113 846.0 0 113 103 242 846.0 0 242 104 127 846.0 0 127 105 54 846.0 0 54 106 57 846.0 0 57 107 257 846.0 0 257 108 267 846.0 0 267 109 167 846.0 0 167 110 184 846.0 0 184 111 149 846.0 0 149 112 136 846.0 0 136 113 161 846.0 0 161 114 213 846.0 0 213 115 189 846.0 0 189 116 277 846.0 0 277 117 335 846.0 0 335 118 193 846.0 0 193 119 248 846.0 0 248 120 272 846.0 0 272 121 146 846.0 0 146 122 179 846.0 0 179 123 255 846.0 0 255 124 268 846.0 0 268 125 298 846.0 0 298 126 867 846.0 0 867 127 789 846.0 0 789 128 339 846.0 0 339 129 181 846.0 0 181 130 126 846.0 0 126 131 192 846.0 0 192 132 218 846.0 0 218 133 237 846.0 0 237 134 368 846.0 0 368 135 351 846.0 0 351 136 357 846.0 0 357 137 298 846.0 0 298 138 182 846.0 0 182 139 182 846.0 0 182 140 305 846.0 0 305 141 177 846.0 0 177 142 146 846.0 0 146 143 215 846.0 0 215 144 260 846.0 0 260 145 263 846.0 0 263 146 197 846.0 0 197 147 156 846.0 0 156 148 288 846.0 0 288 149 190 846.0 0 190 150 231 846.0 0 231 151 198 846.0 0 198 152 239 846.0 0 239 153 449 846.0 0 449 154 327 846.0 0 327 155 398 846.0 0 398 156 392 846.0 0 392 157 699 846.0 0 699 158 414 846.0 0 414 159 216 846.0 0 216 160 251 846.0 0 251 161 179 846.0 0 179 162 155 846.0 0 155 163 225 846.0 0 225 164 308 846.0 0 308 165 378 846.0 0 378 166 234 846.0 0 234 167 214 846.0 0 214 168 236 846.0 0 236 169 356 846.0 0 356 170 410 846.0 0 410 171 256 846.0 0 256 172 412 846.0 0 412 173 724 846.0 0 724 174 436 846.0 0 436 175 475 846.0 0 475 176 332 846.0 0 332 177 284 846.0 0 284 178 362 846.0 0 362 179 316 846.0 0 316 180 377 846.0 0 377 181 358 846.0 0 358 182 250 846.0 0 250 183 229 846.0 0 229 184 213 846.0 0 213 185 339 846.0 0 339 186 241 846.0 0 241 187 142 846.0 0 142 188 135 846.0 0 135 189 383 846.0 0 383 190 614 846.0 0 614 191 501 846.0 0 501 192 353 846.0 0 353 193 302 846.0 0 302 194 137 846.0 0 137 195 294 846.0 0 294 196 386 846.0 0 386 197 803 846.0 0 803 198 583 846.0 0 583 199 188 846.0 0 188 200 224 846.0 0 224 201 272 846.0 0 272 202 617 846.0 0 617 203 596 846.0 0 596 204 274 846.0 0 274 205 229 846.0 0 229 206 263 846.0 0 263 207 241 846.0 0 241 208 129 846.0 0 129 209 155 846.0 0 155 210 156 846.0 0 156 211 167 846.0 0 167 212 214 846.0 0 214 213 264 846.0 0 264 214 246 846.0 0 246 215 285 846.0 0 285 216 461 846.0 0 461 217 880 846.0 0 880 218 451 846.0 0 451 219 300 846.0 0 300 220 245 846.0 0 245 221 217 846.0 0 217 222 294 846.0 0 294 223 300 846.0 0 300 224 307 846.0 0 307 225 364 846.0 0 364 226 422 846.0 0 422 227 437 846.0 0 437 228 534 846.0 0 534 229 1648 846.0 0 1648 230 2353 846.0 0 2353 231 695 846.0 0 695 232 212 846.0 0 212 233 224 846.0 0 224 234 237 846.0 0 237 235 290 846.0 0 290 236 225 846.0 0 225 237 180 846.0 0 180 238 149 846.0 0 149 239 177 846.0 0 177 240 118 846.0 0 118 241 123 846.0 0 123 242 116 846.0 0 116 243 164 846.0 0 164 244 224 846.0 0 224 245 302 846.0 0 302 246 353 846.0 0 353 247 282 846.0 0 282 248 107 846.0 0 107 249 46 846.0 0 46 250 137 846.0 0 137 251 295 846.0 0 295 252 480 846.0 0 480 253 290 846.0 0 290 254 187 846.0 0 187 255 199 846.0 0 199 256 216 846.0 0 216 257 205 846.0 0 205 258 165 846.0 0 165 259 289 846.0 0 289 260 451 846.0 0 451 261 375 846.0 0 375 262 331 846.0 0 331 263 241 846.0 0 241 264 297 846.0 0 297 265 295 846.0 0 295 266 456 846.0 0 456 267 358 846.0 0 358 268 186 846.0 0 186 269 115 846.0 0 115 270 216 846.0 0 216 271 344 846.0 0 344 272 260 846.0 0 260 273 253 846.0 0 253 274 213 846.0 0 213 275 241 846.0 0 241 276 327 846.0 0 327 277 299 846.0 0 299 278 333 846.0 0 333 279 403 846.0 0 403 280 235 846.0 0 235 281 130 846.0 0 130 282 207 846.0 0 207 283 394 846.0 0 394 284 439 846.0 0 439 285 292 846.0 0 292 286 345 846.0 0 345 287 551 846.0 0 551 288 429 846.0 0 429 289 331 846.0 0 331 290 605 846.0 0 605 291 492 846.0 0 492 292 582 846.0 0 582 293 3249 846.0 0 3249 294 3721 846.0 0 3721 295 4005 846.0 0 4005 296 122 846.0 0 122 297 127 846.0 0 127 298 203 846.0 0 203 299 250 846.0 0 250 300 323 846.0 0 323 301 533 846.0 0 533 Finished in 2.44 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 58,464 Reads with adapters: 23,218 (39.7%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 23,204 (39.7%) Total basepairs processed: 12,349,847 bp Total written (filtered): 6,310,098 bp (51.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 23218 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 2 0.0 3 0 0 0 2 18 15 0.0 3 0 0 13 1 1 19 204 0.0 3 0 202 0 2 20 22870 0.0 4 22585 273 7 2 3 21 62 0.0 4 13 47 0 1 1 22 8 0.0 4 0 0 4 1 3 23 9 0.0 4 0 0 0 8 1 24 3 0.0 4 0 0 0 1 2 25 1 0.0 4 0 0 0 0 1 26 1 0.0 4 1 29 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 1 46 4 0.0 4 0 0 0 3 1 53 2 0.0 4 0 0 0 0 2 57 1 0.0 4 0 0 0 1 59 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 111 19 0.0 4 0 0 0 18 1 114 1 0.0 4 0 0 0 1 115 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.79 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 23,204 Reads with adapters: 727 (3.1%) Reads written (passing filters): 23,204 (100.0%) Total basepairs processed: 6,310,098 bp Total written (filtered): 6,289,545 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 727 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.7% C: 7.4% G: 90.0% T: 1.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 362.6 0 1 4 5 90.6 0 5 5 1 22.7 0 1 6 2 5.7 0 2 8 1 0.4 0 1 9 38 0.1 0 0 38 10 484 0.0 1 16 468 11 135 0.0 1 0 135 13 1 0.0 1 0 1 208 1 0.0 1 0 1 223 40 0.0 1 35 5 224 1 0.0 1 1 225 2 0.0 1 2 261 1 0.0 1 1 262 1 0.0 1 1 263 1 0.0 1 1 269 5 0.0 1 5 270 5 0.0 1 5 271 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.