This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 90.91 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 107,509 (3.1%) Reads that were too short: 43,965 (1.3%) Reads written (passing filters): 63,544 (1.8%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 9,812,508 bp (0.9%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 107509 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 24 846.0 0 24 7 40 846.0 0 40 8 38 846.0 0 38 9 1086 846.0 0 1086 10 30 846.0 0 30 11 19 846.0 0 19 12 21 846.0 0 21 13 20 846.0 0 20 14 46 846.0 0 46 15 28 846.0 0 28 16 15 846.0 0 15 17 19 846.0 0 19 18 17 846.0 0 17 19 19 846.0 0 19 20 31 846.0 0 31 21 21 846.0 0 21 22 15 846.0 0 15 23 26 846.0 0 26 24 18 846.0 0 18 25 19 846.0 0 19 26 61 846.0 0 61 27 43 846.0 0 43 28 24 846.0 0 24 29 23 846.0 0 23 30 30 846.0 0 30 31 19 846.0 0 19 32 21 846.0 0 21 33 25 846.0 0 25 34 24 846.0 0 24 35 18 846.0 0 18 36 38 846.0 0 38 37 39 846.0 0 39 38 72 846.0 0 72 39 56 846.0 0 56 40 36 846.0 0 36 41 45 846.0 0 45 42 45 846.0 0 45 43 26 846.0 0 26 44 10 846.0 0 10 45 25 846.0 0 25 46 27 846.0 0 27 47 22 846.0 0 22 48 13 846.0 0 13 49 18 846.0 0 18 50 31 846.0 0 31 51 14 846.0 0 14 52 18 846.0 0 18 53 136 846.0 0 136 54 153 846.0 0 153 55 47 846.0 0 47 56 24 846.0 0 24 57 140 846.0 0 140 58 20 846.0 0 20 59 27 846.0 0 27 60 25 846.0 0 25 61 14 846.0 0 14 62 32 846.0 0 32 63 26 846.0 0 26 64 22 846.0 0 22 65 20 846.0 0 20 66 166 846.0 0 166 67 101 846.0 0 101 68 20 846.0 0 20 69 24 846.0 0 24 70 48 846.0 0 48 71 17 846.0 0 17 72 36 846.0 0 36 73 42 846.0 0 42 74 30 846.0 0 30 75 27 846.0 0 27 76 40 846.0 0 40 77 46 846.0 0 46 78 47 846.0 0 47 79 47 846.0 0 47 80 51 846.0 0 51 81 58 846.0 0 58 82 115 846.0 0 115 83 93 846.0 0 93 84 103 846.0 0 103 85 101 846.0 0 101 86 101 846.0 0 101 87 137 846.0 0 137 88 144 846.0 0 144 89 158 846.0 0 158 90 111 846.0 0 111 91 310 846.0 0 310 92 122 846.0 0 122 93 365 846.0 0 365 94 267 846.0 0 267 95 161 846.0 0 161 96 68 846.0 0 68 97 66 846.0 0 66 98 88 846.0 0 88 99 99 846.0 0 99 100 64 846.0 0 64 101 154 846.0 0 154 102 211 846.0 0 211 103 274 846.0 0 274 104 253 846.0 0 253 105 292 846.0 0 292 106 121 846.0 0 121 107 240 846.0 0 240 108 94 846.0 0 94 109 137 846.0 0 137 110 83 846.0 0 83 111 443 846.0 0 443 112 353 846.0 0 353 113 467 846.0 0 467 114 423 846.0 0 423 115 209 846.0 0 209 116 119 846.0 0 119 117 123 846.0 0 123 118 109 846.0 0 109 119 127 846.0 0 127 120 187 846.0 0 187 121 152 846.0 0 152 122 124 846.0 0 124 123 207 846.0 0 207 124 212 846.0 0 212 125 1414 846.0 0 1414 126 770 846.0 0 770 127 1309 846.0 0 1309 128 146 846.0 0 146 129 208 846.0 0 208 130 300 846.0 0 300 131 193 846.0 0 193 132 282 846.0 0 282 133 322 846.0 0 322 134 512 846.0 0 512 135 228 846.0 0 228 136 159 846.0 0 159 137 256 846.0 0 256 138 398 846.0 0 398 139 410 846.0 0 410 140 345 846.0 0 345 141 262 846.0 0 262 142 188 846.0 0 188 143 242 846.0 0 242 144 619 846.0 0 619 145 661 846.0 0 661 146 685 846.0 0 685 147 345 846.0 0 345 148 278 846.0 0 278 149 311 846.0 0 311 150 786 846.0 0 786 151 2097 846.0 0 2097 152 600 846.0 0 600 153 412 846.0 0 412 154 525 846.0 0 525 155 4197 846.0 0 4197 156 644 846.0 0 644 157 739 846.0 0 739 158 791 846.0 0 791 159 995 846.0 0 995 160 5138 846.0 0 5138 161 3098 846.0 0 3098 162 2491 846.0 0 2491 163 474 846.0 0 474 164 729 846.0 0 729 165 741 846.0 0 741 166 469 846.0 0 469 167 277 846.0 0 277 168 348 846.0 0 348 169 446 846.0 0 446 170 362 846.0 0 362 171 289 846.0 0 289 172 291 846.0 0 291 173 322 846.0 0 322 174 319 846.0 0 319 175 349 846.0 0 349 176 370 846.0 0 370 177 477 846.0 0 477 178 444 846.0 0 444 179 559 846.0 0 559 180 2372 846.0 0 2372 181 1463 846.0 0 1463 182 1291 846.0 0 1291 183 421 846.0 0 421 184 330 846.0 0 330 185 388 846.0 0 388 186 538 846.0 0 538 187 352 846.0 0 352 188 288 846.0 0 288 189 415 846.0 0 415 190 426 846.0 0 426 191 382 846.0 0 382 192 219 846.0 0 219 193 179 846.0 0 179 194 139 846.0 0 139 195 221 846.0 0 221 196 309 846.0 0 309 197 263 846.0 0 263 198 229 846.0 0 229 199 278 846.0 0 278 200 295 846.0 0 295 201 476 846.0 0 476 202 268 846.0 0 268 203 184 846.0 0 184 204 223 846.0 0 223 205 249 846.0 0 249 206 308 846.0 0 308 207 352 846.0 0 352 208 406 846.0 0 406 209 304 846.0 0 304 210 315 846.0 0 315 211 545 846.0 0 545 212 1506 846.0 0 1506 213 1124 846.0 0 1124 214 1089 846.0 0 1089 215 373 846.0 0 373 216 469 846.0 0 469 217 464 846.0 0 464 218 441 846.0 0 441 219 290 846.0 0 290 220 378 846.0 0 378 221 466 846.0 0 466 222 419 846.0 0 419 223 367 846.0 0 367 224 478 846.0 0 478 225 663 846.0 0 663 226 465 846.0 0 465 227 482 846.0 0 482 228 323 846.0 0 323 229 425 846.0 0 425 230 348 846.0 0 348 231 361 846.0 0 361 232 281 846.0 0 281 233 336 846.0 0 336 234 343 846.0 0 343 235 239 846.0 0 239 236 266 846.0 0 266 237 403 846.0 0 403 238 368 846.0 0 368 239 394 846.0 0 394 240 428 846.0 0 428 241 606 846.0 0 606 242 480 846.0 0 480 243 548 846.0 0 548 244 462 846.0 0 462 245 588 846.0 0 588 246 487 846.0 0 487 247 523 846.0 0 523 248 329 846.0 0 329 249 259 846.0 0 259 250 430 846.0 0 430 251 401 846.0 0 401 252 299 846.0 0 299 253 351 846.0 0 351 254 629 846.0 0 629 255 770 846.0 0 770 256 1069 846.0 0 1069 257 951 846.0 0 951 258 409 846.0 0 409 259 249 846.0 0 249 260 311 846.0 0 311 261 321 846.0 0 321 262 336 846.0 0 336 263 303 846.0 0 303 264 364 846.0 0 364 265 358 846.0 0 358 266 941 846.0 0 941 267 646 846.0 0 646 268 566 846.0 0 566 269 416 846.0 0 416 270 470 846.0 0 470 271 420 846.0 0 420 272 421 846.0 0 421 273 705 846.0 0 705 274 549 846.0 0 549 275 561 846.0 0 561 276 616 846.0 0 616 277 870 846.0 0 870 278 318 846.0 0 318 279 249 846.0 0 249 280 481 846.0 0 481 281 538 846.0 0 538 282 935 846.0 0 935 283 616 846.0 0 616 284 523 846.0 0 523 285 360 846.0 0 360 286 196 846.0 0 196 287 401 846.0 0 401 288 567 846.0 0 567 289 515 846.0 0 515 290 242 846.0 0 242 291 263 846.0 0 263 292 160 846.0 0 160 293 204 846.0 0 204 294 163 846.0 0 163 295 153 846.0 0 153 296 153 846.0 0 153 297 159 846.0 0 159 298 190 846.0 0 190 299 116 846.0 0 116 300 167 846.0 0 167 301 139 846.0 0 139 Finished in 1.98 s (31 us/read; 1.93 M reads/minute). === Summary === Total reads processed: 63,544 Reads with adapters: 1,143 (1.8%) Reads that were too short: 48 (0.1%) Reads written (passing filters): 1,095 (1.7%) Total basepairs processed: 9,812,508 bp Total written (filtered): 298,593 bp (3.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1143 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 16 9 0.0 3 0 8 1 17 1053 0.0 3 1015 33 4 1 18 11 0.0 3 2 5 3 1 19 1 0.0 3 0 0 0 1 20 2 0.0 3 0 0 0 2 23 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 35 7 0.0 3 0 0 0 7 37 4 0.0 3 0 0 0 4 38 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 56 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 72 2 0.0 3 0 0 0 2 77 1 0.0 3 0 0 1 80 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 111 2 0.0 3 0 0 0 2 113 5 0.0 3 0 0 1 4 114 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 0 2 154 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 2 0.0 3 0 0 0 2 166 2 0.0 3 0 0 0 2 167 1 0.0 3 0 0 0 1 168 2 0.0 3 0 0 0 2 202 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.04 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 1,095 Reads with adapters: 35 (3.2%) Reads written (passing filters): 1,095 (100.0%) Total basepairs processed: 298,593 bp Total written (filtered): 298,486 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 35 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 45.7% C: 5.7% G: 22.9% T: 25.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 33 17.1 0 33 4 2 4.3 0 2