This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AACAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 91.84 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 876,447 (25.3%) Reads that were too short: 457,986 (13.2%) Reads written (passing filters): 418,461 (12.1%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 70,187,146 bp (6.7%) === Adapter 1 === Sequence: AACAAC; Type: regular 5'; Length: 6; Trimmed: 876447 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 246 846.0 0 246 7 82 846.0 0 82 8 1202 846.0 0 1202 9 31 846.0 0 31 10 60 846.0 0 60 11 50 846.0 0 50 12 47 846.0 0 47 13 50 846.0 0 50 14 46 846.0 0 46 15 49 846.0 0 49 16 48 846.0 0 48 17 39 846.0 0 39 18 46 846.0 0 46 19 55 846.0 0 55 20 34 846.0 0 34 21 46 846.0 0 46 22 34 846.0 0 34 23 44 846.0 0 44 24 35 846.0 0 35 25 27 846.0 0 27 26 42 846.0 0 42 27 28 846.0 0 28 28 32 846.0 0 32 29 35 846.0 0 35 30 44 846.0 0 44 31 33 846.0 0 33 32 46 846.0 0 46 33 46 846.0 0 46 34 55 846.0 0 55 35 44 846.0 0 44 36 39 846.0 0 39 37 40 846.0 0 40 38 39 846.0 0 39 39 38 846.0 0 38 40 43 846.0 0 43 41 49 846.0 0 49 42 38 846.0 0 38 43 193 846.0 0 193 44 113 846.0 0 113 45 44 846.0 0 44 46 35 846.0 0 35 47 48 846.0 0 48 48 47 846.0 0 47 49 34 846.0 0 34 50 51 846.0 0 51 51 44 846.0 0 44 52 33 846.0 0 33 53 39 846.0 0 39 54 43 846.0 0 43 55 54 846.0 0 54 56 40 846.0 0 40 57 48 846.0 0 48 58 34 846.0 0 34 59 44 846.0 0 44 60 40 846.0 0 40 61 40 846.0 0 40 62 44 846.0 0 44 63 42 846.0 0 42 64 40 846.0 0 40 65 93 846.0 0 93 66 37 846.0 0 37 67 96 846.0 0 96 68 76 846.0 0 76 69 53 846.0 0 53 70 118 846.0 0 118 71 93 846.0 0 93 72 78 846.0 0 78 73 55 846.0 0 55 74 71 846.0 0 71 75 90 846.0 0 90 76 138 846.0 0 138 77 225 846.0 0 225 78 1216 846.0 0 1216 79 4205 846.0 0 4205 80 3983 846.0 0 3983 81 6293 846.0 0 6293 82 6079 846.0 0 6079 83 6517 846.0 0 6517 84 5613 846.0 0 5613 85 3918 846.0 0 3918 86 3930 846.0 0 3930 87 4772 846.0 0 4772 88 2996 846.0 0 2996 89 4969 846.0 0 4969 90 6138 846.0 0 6138 91 7034 846.0 0 7034 92 6491 846.0 0 6491 93 3787 846.0 0 3787 94 4405 846.0 0 4405 95 1842 846.0 0 1842 96 1434 846.0 0 1434 97 2374 846.0 0 2374 98 1451 846.0 0 1451 99 1317 846.0 0 1317 100 3090 846.0 0 3090 101 3117 846.0 0 3117 102 2077 846.0 0 2077 103 3108 846.0 0 3108 104 3894 846.0 0 3894 105 5985 846.0 0 5985 106 2873 846.0 0 2873 107 3206 846.0 0 3206 108 4471 846.0 0 4471 109 3358 846.0 0 3358 110 4133 846.0 0 4133 111 3757 846.0 0 3757 112 3272 846.0 0 3272 113 3016 846.0 0 3016 114 3788 846.0 0 3788 115 3488 846.0 0 3488 116 3202 846.0 0 3202 117 3257 846.0 0 3257 118 3218 846.0 0 3218 119 3069 846.0 0 3069 120 3765 846.0 0 3765 121 3974 846.0 0 3974 122 3142 846.0 0 3142 123 3109 846.0 0 3109 124 3632 846.0 0 3632 125 4130 846.0 0 4130 126 4157 846.0 0 4157 127 3326 846.0 0 3326 128 3118 846.0 0 3118 129 2896 846.0 0 2896 130 2732 846.0 0 2732 131 3049 846.0 0 3049 132 3389 846.0 0 3389 133 2884 846.0 0 2884 134 2907 846.0 0 2907 135 2708 846.0 0 2708 136 2744 846.0 0 2744 137 2888 846.0 0 2888 138 2724 846.0 0 2724 139 2460 846.0 0 2460 140 2947 846.0 0 2947 141 3329 846.0 0 3329 142 3207 846.0 0 3207 143 3204 846.0 0 3204 144 3266 846.0 0 3266 145 3806 846.0 0 3806 146 3694 846.0 0 3694 147 3730 846.0 0 3730 148 3372 846.0 0 3372 149 4215 846.0 0 4215 150 4524 846.0 0 4524 151 3878 846.0 0 3878 152 4170 846.0 0 4170 153 4175 846.0 0 4175 154 3697 846.0 0 3697 155 3018 846.0 0 3018 156 3202 846.0 0 3202 157 2858 846.0 0 2858 158 2933 846.0 0 2933 159 2921 846.0 0 2921 160 3015 846.0 0 3015 161 3654 846.0 0 3654 162 3789 846.0 0 3789 163 3413 846.0 0 3413 164 2621 846.0 0 2621 165 2604 846.0 0 2604 166 2554 846.0 0 2554 167 2509 846.0 0 2509 168 2765 846.0 0 2765 169 3377 846.0 0 3377 170 3643 846.0 0 3643 171 3785 846.0 0 3785 172 3157 846.0 0 3157 173 3160 846.0 0 3160 174 3446 846.0 0 3446 175 2650 846.0 0 2650 176 2551 846.0 0 2551 177 3235 846.0 0 3235 178 3377 846.0 0 3377 179 3168 846.0 0 3168 180 2756 846.0 0 2756 181 2914 846.0 0 2914 182 2986 846.0 0 2986 183 2999 846.0 0 2999 184 2192 846.0 0 2192 185 2054 846.0 0 2054 186 2473 846.0 0 2473 187 2942 846.0 0 2942 188 2765 846.0 0 2765 189 2778 846.0 0 2778 190 2967 846.0 0 2967 191 2855 846.0 0 2855 192 2391 846.0 0 2391 193 2083 846.0 0 2083 194 3077 846.0 0 3077 195 2524 846.0 0 2524 196 1722 846.0 0 1722 197 2483 846.0 0 2483 198 2336 846.0 0 2336 199 2697 846.0 0 2697 200 2603 846.0 0 2603 201 2033 846.0 0 2033 202 2286 846.0 0 2286 203 2486 846.0 0 2486 204 2427 846.0 0 2427 205 1891 846.0 0 1891 206 1688 846.0 0 1688 207 1882 846.0 0 1882 208 2090 846.0 0 2090 209 1965 846.0 0 1965 210 1694 846.0 0 1694 211 1993 846.0 0 1993 212 2299 846.0 0 2299 213 2941 846.0 0 2941 214 2951 846.0 0 2951 215 2765 846.0 0 2765 216 2251 846.0 0 2251 217 2429 846.0 0 2429 218 3022 846.0 0 3022 219 2135 846.0 0 2135 220 2505 846.0 0 2505 221 2222 846.0 0 2222 222 1794 846.0 0 1794 223 2354 846.0 0 2354 224 2539 846.0 0 2539 225 2956 846.0 0 2956 226 4110 846.0 0 4110 227 3687 846.0 0 3687 228 3821 846.0 0 3821 229 3265 846.0 0 3265 230 4861 846.0 0 4861 231 3954 846.0 0 3954 232 3001 846.0 0 3001 233 2183 846.0 0 2183 234 2035 846.0 0 2035 235 2030 846.0 0 2030 236 1963 846.0 0 1963 237 1896 846.0 0 1896 238 1557 846.0 0 1557 239 2162 846.0 0 2162 240 2080 846.0 0 2080 241 1892 846.0 0 1892 242 1728 846.0 0 1728 243 2229 846.0 0 2229 244 2096 846.0 0 2096 245 1616 846.0 0 1616 246 1684 846.0 0 1684 247 2394 846.0 0 2394 248 2635 846.0 0 2635 249 2922 846.0 0 2922 250 2687 846.0 0 2687 251 2708 846.0 0 2708 252 3590 846.0 0 3590 253 3153 846.0 0 3153 254 2026 846.0 0 2026 255 1619 846.0 0 1619 256 1641 846.0 0 1641 257 1804 846.0 0 1804 258 1841 846.0 0 1841 259 2407 846.0 0 2407 260 2363 846.0 0 2363 261 2849 846.0 0 2849 262 3996 846.0 0 3996 263 5859 846.0 0 5859 264 23614 846.0 0 23614 265 188573 846.0 0 188573 266 11386 846.0 0 11386 267 2425 846.0 0 2425 268 3533 846.0 0 3533 269 2338 846.0 0 2338 270 2120 846.0 0 2120 271 2388 846.0 0 2388 272 2795 846.0 0 2795 273 2182 846.0 0 2182 274 1925 846.0 0 1925 275 2417 846.0 0 2417 276 2463 846.0 0 2463 277 1930 846.0 0 1930 278 2105 846.0 0 2105 279 1994 846.0 0 1994 280 2681 846.0 0 2681 281 2190 846.0 0 2190 282 1368 846.0 0 1368 283 2300 846.0 0 2300 284 2471 846.0 0 2471 285 1692 846.0 0 1692 286 1455 846.0 0 1455 287 2012 846.0 0 2012 288 4288 846.0 0 4288 289 1981 846.0 0 1981 290 2499 846.0 0 2499 291 2894 846.0 0 2894 292 2235 846.0 0 2235 293 1685 846.0 0 1685 294 1907 846.0 0 1907 295 1694 846.0 0 1694 296 2067 846.0 0 2067 297 2806 846.0 0 2806 298 2460 846.0 0 2460 299 2292 846.0 0 2292 300 2220 846.0 0 2220 301 692 846.0 0 692 Finished in 13.93 s (33 us/read; 1.80 M reads/minute). === Summary === Total reads processed: 418,461 Reads with adapters: 1,579 (0.4%) Reads that were too short: 178 (0.0%) Reads written (passing filters): 1,401 (0.3%) Total basepairs processed: 70,187,146 bp Total written (filtered): 374,138 bp (0.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1579 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 1 2 18 3 0.0 3 0 0 1 0 2 19 11 0.0 3 0 11 20 1125 0.0 4 1111 12 0 0 2 21 5 0.0 4 1 3 0 0 1 22 2 0.0 4 2 23 188 0.0 4 180 5 1 2 24 1 0.0 4 0 0 0 0 1 26 2 0.0 4 0 0 0 0 2 27 1 0.0 4 0 0 0 0 1 28 1 0.0 4 1 29 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 0 0 1 31 2 0.0 4 0 0 0 0 2 32 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 34 3 0.0 4 0 0 0 0 3 35 2 0.0 4 0 0 0 0 2 36 4 0.0 4 0 0 0 1 3 37 3 0.0 4 0 0 0 0 3 40 2 0.0 4 0 0 0 0 2 41 2 0.0 4 0 0 0 0 2 42 1 0.0 4 0 0 0 0 1 43 2 0.0 4 0 0 0 0 2 44 2 0.0 4 0 0 0 0 2 45 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 47 3 0.0 4 0 0 0 0 3 49 1 0.0 4 0 0 0 0 1 52 4 0.0 4 0 0 0 2 2 53 1 0.0 4 0 0 0 0 1 54 3 0.0 4 0 0 0 0 3 55 5 0.0 4 0 0 0 2 3 56 1 0.0 4 0 0 0 0 1 57 2 0.0 4 0 0 0 0 2 60 3 0.0 4 0 0 0 0 3 61 1 0.0 4 0 0 0 0 1 63 2 0.0 4 0 0 0 0 2 64 2 0.0 4 0 0 0 0 2 65 3 0.0 4 0 0 0 0 3 66 1 0.0 4 0 0 0 0 1 67 2 0.0 4 0 0 0 0 2 68 4 0.0 4 0 0 0 0 4 69 1 0.0 4 0 0 0 0 1 70 2 0.0 4 0 0 0 0 2 71 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 74 2 0.0 4 0 0 0 0 2 75 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 1 78 1 0.0 4 0 0 0 0 1 79 2 0.0 4 0 0 0 0 2 80 2 0.0 4 0 0 0 0 2 81 5 0.0 4 0 0 0 0 5 82 1 0.0 4 0 0 0 0 1 83 3 0.0 4 0 0 0 0 3 84 2 0.0 4 0 0 0 0 2 85 2 0.0 4 0 0 0 0 2 86 3 0.0 4 0 0 0 0 3 87 3 0.0 4 0 0 0 0 3 88 1 0.0 4 0 0 0 1 89 3 0.0 4 0 0 0 0 3 91 1 0.0 4 0 0 0 1 93 5 0.0 4 0 0 0 0 5 94 2 0.0 4 0 0 0 0 2 96 2 0.0 4 0 0 0 0 2 97 1 0.0 4 0 0 0 0 1 98 3 0.0 4 0 0 0 0 3 99 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 105 3 0.0 4 0 0 0 0 3 107 2 0.0 4 0 0 0 0 2 108 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 110 4 0.0 4 0 0 0 1 3 111 18 0.0 4 0 0 0 17 1 112 1 0.0 4 0 0 0 0 1 113 2 0.0 4 0 0 0 0 2 114 1 0.0 4 0 0 0 1 115 2 0.0 4 0 0 0 0 2 116 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 118 2 0.0 4 0 0 0 0 2 119 3 0.0 4 0 0 0 0 3 120 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 123 2 0.0 4 0 0 0 0 2 124 2 0.0 4 0 0 0 0 2 125 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 2 0.0 4 0 0 0 0 2 129 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 131 2 0.0 4 0 0 0 0 2 132 3 0.0 4 0 0 0 0 3 133 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 136 2 0.0 4 0 0 0 0 2 139 1 0.0 4 0 0 0 0 1 141 3 0.0 4 0 0 0 0 3 144 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 146 2 0.0 4 0 0 0 0 2 147 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 151 3 0.0 4 0 0 0 0 3 152 1 0.0 4 0 0 0 0 1 154 2 0.0 4 0 0 0 0 2 158 1 0.0 4 0 0 0 0 1 162 3 0.0 4 0 0 0 0 3 163 3 0.0 4 0 0 0 0 3 165 3 0.0 4 0 0 0 0 3 167 1 0.0 4 0 0 0 0 1 169 2 0.0 4 0 0 0 0 2 170 2 0.0 4 0 0 0 0 2 171 1 0.0 4 0 0 0 1 177 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 1 179 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.05 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 1,401 Reads with adapters: 39 (2.8%) Reads written (passing filters): 1,401 (100.0%) Total basepairs processed: 374,138 bp Total written (filtered): 370,890 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 39 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 12.8% C: 12.8% G: 69.2% T: 5.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 10 3 0.0 1 0 3 11 24 0.0 1 0 24 123 1 0.0 1 1 209 1 0.0 1 0 1 254 1 0.0 1 1 258 2 0.0 1 1 1 260 1 0.0 1 1 262 2 0.0 1 2 263 2 0.0 1 2 271 2 0.0 1 2