This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 89.09 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 53,809 (1.6%) Reads that were too short: 4,961 (0.1%) Reads written (passing filters): 48,848 (1.4%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 11,807,122 bp (1.1%) === Adapter 1 === Sequence: GTGCCT; Type: regular 5'; Length: 6; Trimmed: 53809 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 356 846.0 0 356 7 1458 846.0 0 1458 8 5284 846.0 0 5284 9 48 846.0 0 48 10 31 846.0 0 31 11 12 846.0 0 12 12 25 846.0 0 25 13 42 846.0 0 42 14 13 846.0 0 13 15 20 846.0 0 20 16 6 846.0 0 6 17 18 846.0 0 18 18 26 846.0 0 26 19 19 846.0 0 19 20 16 846.0 0 16 21 25 846.0 0 25 22 25 846.0 0 25 23 23 846.0 0 23 24 19 846.0 0 19 25 16 846.0 0 16 26 7 846.0 0 7 27 13 846.0 0 13 28 45 846.0 0 45 29 19 846.0 0 19 30 15 846.0 0 15 31 17 846.0 0 17 32 16 846.0 0 16 33 16 846.0 0 16 34 25 846.0 0 25 35 28 846.0 0 28 36 39 846.0 0 39 37 50 846.0 0 50 38 363 846.0 0 363 39 660 846.0 0 660 40 69 846.0 0 69 41 413 846.0 0 413 42 44 846.0 0 44 43 158 846.0 0 158 44 64 846.0 0 64 45 45 846.0 0 45 46 114 846.0 0 114 47 298 846.0 0 298 48 80 846.0 0 80 49 389 846.0 0 389 50 120 846.0 0 120 51 151 846.0 0 151 52 189 846.0 0 189 53 427 846.0 0 427 54 575 846.0 0 575 55 12893 846.0 0 12893 56 6514 846.0 0 6514 57 7669 846.0 0 7669 58 69 846.0 0 69 59 14 846.0 0 14 60 14 846.0 0 14 61 12 846.0 0 12 62 12 846.0 0 12 63 18 846.0 0 18 64 12 846.0 0 12 65 16 846.0 0 16 66 15 846.0 0 15 67 19 846.0 0 19 68 28 846.0 0 28 69 11 846.0 0 11 70 23 846.0 0 23 71 16 846.0 0 16 72 23 846.0 0 23 73 34 846.0 0 34 74 28 846.0 0 28 75 27 846.0 0 27 76 29 846.0 0 29 77 28 846.0 0 28 78 20 846.0 0 20 79 20 846.0 0 20 80 32 846.0 0 32 81 48 846.0 0 48 82 56 846.0 0 56 83 66 846.0 0 66 84 74 846.0 0 74 85 1978 846.0 0 1978 86 787 846.0 0 787 87 1588 846.0 0 1588 88 51 846.0 0 51 89 81 846.0 0 81 90 25 846.0 0 25 91 21 846.0 0 21 92 27 846.0 0 27 93 33 846.0 0 33 94 54 846.0 0 54 95 20 846.0 0 20 96 25 846.0 0 25 97 23 846.0 0 23 98 12 846.0 0 12 99 15 846.0 0 15 100 21 846.0 0 21 101 27 846.0 0 27 102 90 846.0 0 90 103 94 846.0 0 94 104 32 846.0 0 32 105 24 846.0 0 24 106 13 846.0 0 13 107 11 846.0 0 11 108 13 846.0 0 13 109 16 846.0 0 16 110 23 846.0 0 23 111 37 846.0 0 37 112 22 846.0 0 22 113 18 846.0 0 18 114 13 846.0 0 13 115 20 846.0 0 20 116 16 846.0 0 16 117 20 846.0 0 20 118 68 846.0 0 68 119 48 846.0 0 48 120 21 846.0 0 21 121 20 846.0 0 20 122 21 846.0 0 21 123 40 846.0 0 40 124 43 846.0 0 43 125 32 846.0 0 32 126 127 846.0 0 127 127 114 846.0 0 114 128 98 846.0 0 98 129 24 846.0 0 24 130 37 846.0 0 37 131 76 846.0 0 76 132 46 846.0 0 46 133 60 846.0 0 60 134 33 846.0 0 33 135 29 846.0 0 29 136 27 846.0 0 27 137 125 846.0 0 125 138 96 846.0 0 96 139 135 846.0 0 135 140 46 846.0 0 46 141 25 846.0 0 25 142 28 846.0 0 28 143 22 846.0 0 22 144 21 846.0 0 21 145 23 846.0 0 23 146 47 846.0 0 47 147 13 846.0 0 13 148 33 846.0 0 33 149 28 846.0 0 28 150 24 846.0 0 24 151 37 846.0 0 37 152 23 846.0 0 23 153 32 846.0 0 32 154 37 846.0 0 37 155 16 846.0 0 16 156 29 846.0 0 29 157 119 846.0 0 119 158 70 846.0 0 70 159 89 846.0 0 89 160 35 846.0 0 35 161 31 846.0 0 31 162 101 846.0 0 101 163 29 846.0 0 29 164 43 846.0 0 43 165 34 846.0 0 34 166 36 846.0 0 36 167 76 846.0 0 76 168 41 846.0 0 41 169 37 846.0 0 37 170 32 846.0 0 32 171 30 846.0 0 30 172 39 846.0 0 39 173 18 846.0 0 18 174 19 846.0 0 19 175 25 846.0 0 25 176 27 846.0 0 27 177 24 846.0 0 24 178 17 846.0 0 17 179 22 846.0 0 22 180 31 846.0 0 31 181 40 846.0 0 40 182 29 846.0 0 29 183 46 846.0 0 46 184 36 846.0 0 36 185 33 846.0 0 33 186 23 846.0 0 23 187 128 846.0 0 128 188 135 846.0 0 135 189 75 846.0 0 75 190 84 846.0 0 84 191 66 846.0 0 66 192 27 846.0 0 27 193 24 846.0 0 24 194 27 846.0 0 27 195 28 846.0 0 28 196 18 846.0 0 18 197 18 846.0 0 18 198 21 846.0 0 21 199 40 846.0 0 40 200 75 846.0 0 75 201 25 846.0 0 25 202 20 846.0 0 20 203 17 846.0 0 17 204 24 846.0 0 24 205 39 846.0 0 39 206 56 846.0 0 56 207 56 846.0 0 56 208 35 846.0 0 35 209 26 846.0 0 26 210 19 846.0 0 19 211 21 846.0 0 21 212 33 846.0 0 33 213 64 846.0 0 64 214 39 846.0 0 39 215 46 846.0 0 46 216 42 846.0 0 42 217 41 846.0 0 41 218 55 846.0 0 55 219 274 846.0 0 274 220 1087 846.0 0 1087 221 71 846.0 0 71 222 30 846.0 0 30 223 29 846.0 0 29 224 27 846.0 0 27 225 47 846.0 0 47 226 45 846.0 0 45 227 23 846.0 0 23 228 28 846.0 0 28 229 33 846.0 0 33 230 17 846.0 0 17 231 21 846.0 0 21 232 16 846.0 0 16 233 25 846.0 0 25 234 22 846.0 0 22 235 33 846.0 0 33 236 34 846.0 0 34 237 9 846.0 0 9 238 37 846.0 0 37 239 35 846.0 0 35 240 53 846.0 0 53 241 27 846.0 0 27 242 26 846.0 0 26 243 25 846.0 0 25 244 36 846.0 0 36 245 41 846.0 0 41 246 38 846.0 0 38 247 59 846.0 0 59 248 12 846.0 0 12 249 21 846.0 0 21 250 17 846.0 0 17 251 31 846.0 0 31 252 19 846.0 0 19 253 34 846.0 0 34 254 23 846.0 0 23 255 32 846.0 0 32 256 28 846.0 0 28 257 106 846.0 0 106 258 27 846.0 0 27 259 28 846.0 0 28 260 16 846.0 0 16 261 27 846.0 0 27 262 46 846.0 0 46 263 10 846.0 0 10 264 22 846.0 0 22 265 39 846.0 0 39 266 34 846.0 0 34 267 29 846.0 0 29 268 21 846.0 0 21 269 25 846.0 0 25 270 14 846.0 0 14 271 11 846.0 0 11 272 22 846.0 0 22 273 19 846.0 0 19 274 23 846.0 0 23 275 34 846.0 0 34 276 148 846.0 0 148 277 37 846.0 0 37 278 311 846.0 0 311 279 28 846.0 0 28 280 40 846.0 0 40 281 60 846.0 0 60 282 30 846.0 0 30 283 40 846.0 0 40 284 15 846.0 0 15 285 30 846.0 0 30 286 17 846.0 0 17 287 48 846.0 0 48 288 25 846.0 0 25 289 34 846.0 0 34 290 13 846.0 0 13 291 22 846.0 0 22 292 27 846.0 0 27 293 16 846.0 0 16 294 35 846.0 0 35 295 27 846.0 0 27 296 78 846.0 0 78 297 60 846.0 0 60 298 42 846.0 0 42 299 60 846.0 0 60 300 56 846.0 0 56 301 11 846.0 0 11 Finished in 1.98 s (41 us/read; 1.48 M reads/minute). === Summary === Total reads processed: 48,848 Reads with adapters: 1,474 (3.0%) Reads that were too short: 42 (0.1%) Reads written (passing filters): 1,432 (2.9%) Total basepairs processed: 11,807,122 bp Total written (filtered): 394,670 bp (3.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1474 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 5 0.0 3 0 3 0 2 17 1398 0.0 3 1364 27 5 2 18 9 0.0 3 5 2 2 19 3 0.0 3 0 0 1 2 20 2 0.0 3 2 22 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 34 3 0.0 3 0 0 0 3 59 2 0.0 3 0 0 0 2 60 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 2 0.0 3 0 0 0 2 127 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 3 0.0 3 0 0 0 3 153 1 0.0 3 0 0 0 1 169 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 190 7 0.0 3 0 0 0 7 203 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 217 2 0.0 3 0 0 0 2 223 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 234 2 0.0 3 0 0 0 2 235 2 0.0 3 0 0 0 2 236 2 0.0 3 0 0 0 2 238 2 0.0 3 0 0 0 2 242 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 283 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 1,432 Reads with adapters: 134 (9.4%) Reads written (passing filters): 1,432 (100.0%) Total basepairs processed: 394,670 bp Total written (filtered): 394,266 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 134 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 21.6% C: 76.9% G: 1.5% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 133 22.4 0 133 5 1 1.4 0 1