This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GACGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 91.60 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 444,951 (12.8%) Reads that were too short: 36,202 (1.0%) Reads written (passing filters): 408,749 (11.8%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 92,019,480 bp (8.8%) === Adapter 1 === Sequence: GACGGC; Type: regular 5'; Length: 6; Trimmed: 444951 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 672 846.0 0 672 7 3484 846.0 0 3484 8 916 846.0 0 916 9 90630 846.0 0 90630 10 289 846.0 0 289 11 31 846.0 0 31 12 34 846.0 0 34 13 45 846.0 0 45 14 44 846.0 0 44 15 53 846.0 0 53 16 51 846.0 0 51 17 38 846.0 0 38 18 52 846.0 0 52 19 40 846.0 0 40 20 66 846.0 0 66 21 45 846.0 0 45 22 45 846.0 0 45 23 35 846.0 0 35 24 47 846.0 0 47 25 44 846.0 0 44 26 37 846.0 0 37 27 58 846.0 0 58 28 47 846.0 0 47 29 32 846.0 0 32 30 38 846.0 0 38 31 38 846.0 0 38 32 41 846.0 0 41 33 42 846.0 0 42 34 37 846.0 0 37 35 64 846.0 0 64 36 57 846.0 0 57 37 34 846.0 0 34 38 36 846.0 0 36 39 43 846.0 0 43 40 42 846.0 0 42 41 53 846.0 0 53 42 65 846.0 0 65 43 62 846.0 0 62 44 47 846.0 0 47 45 61 846.0 0 61 46 55 846.0 0 55 47 77 846.0 0 77 48 74 846.0 0 74 49 51 846.0 0 51 50 81 846.0 0 81 51 67 846.0 0 67 52 49 846.0 0 49 53 78 846.0 0 78 54 46 846.0 0 46 55 53 846.0 0 53 56 46 846.0 0 46 57 53 846.0 0 53 58 58 846.0 0 58 59 91 846.0 0 91 60 223 846.0 0 223 61 351 846.0 0 351 62 297 846.0 0 297 63 97 846.0 0 97 64 70 846.0 0 70 65 175 846.0 0 175 66 84 846.0 0 84 67 140 846.0 0 140 68 231 846.0 0 231 69 407 846.0 0 407 70 717 846.0 0 717 71 2038 846.0 0 2038 72 2569 846.0 0 2569 73 587 846.0 0 587 74 561 846.0 0 561 75 1181 846.0 0 1181 76 1217 846.0 0 1217 77 840 846.0 0 840 78 1025 846.0 0 1025 79 981 846.0 0 981 80 1178 846.0 0 1178 81 1339 846.0 0 1339 82 1922 846.0 0 1922 83 2241 846.0 0 2241 84 4403 846.0 0 4403 85 123905 846.0 0 123905 86 34006 846.0 0 34006 87 83572 846.0 0 83572 88 481 846.0 0 481 89 46 846.0 0 46 90 85 846.0 0 85 91 49 846.0 0 49 92 40 846.0 0 40 93 81 846.0 0 81 94 47 846.0 0 47 95 39 846.0 0 39 96 38 846.0 0 38 97 39 846.0 0 39 98 40 846.0 0 40 99 30 846.0 0 30 100 41 846.0 0 41 101 35 846.0 0 35 102 37 846.0 0 37 103 35 846.0 0 35 104 29 846.0 0 29 105 24 846.0 0 24 106 23 846.0 0 23 107 43 846.0 0 43 108 32 846.0 0 32 109 20 846.0 0 20 110 21 846.0 0 21 111 38 846.0 0 38 112 25 846.0 0 25 113 31 846.0 0 31 114 44 846.0 0 44 115 53 846.0 0 53 116 29 846.0 0 29 117 35 846.0 0 35 118 41 846.0 0 41 119 35 846.0 0 35 120 39 846.0 0 39 121 61 846.0 0 61 122 43 846.0 0 43 123 49 846.0 0 49 124 60 846.0 0 60 125 49 846.0 0 49 126 43 846.0 0 43 127 44 846.0 0 44 128 278 846.0 0 278 129 66 846.0 0 66 130 72 846.0 0 72 131 75 846.0 0 75 132 143 846.0 0 143 133 88 846.0 0 88 134 53 846.0 0 53 135 69 846.0 0 69 136 85 846.0 0 85 137 77 846.0 0 77 138 66 846.0 0 66 139 50 846.0 0 50 140 73 846.0 0 73 141 46 846.0 0 46 142 75 846.0 0 75 143 74 846.0 0 74 144 53 846.0 0 53 145 66 846.0 0 66 146 67 846.0 0 67 147 64 846.0 0 64 148 84 846.0 0 84 149 340 846.0 0 340 150 699 846.0 0 699 151 704 846.0 0 704 152 867 846.0 0 867 153 554 846.0 0 554 154 291 846.0 0 291 155 390 846.0 0 390 156 142 846.0 0 142 157 83 846.0 0 83 158 78 846.0 0 78 159 74 846.0 0 74 160 111 846.0 0 111 161 182 846.0 0 182 162 312 846.0 0 312 163 109 846.0 0 109 164 154 846.0 0 154 165 208 846.0 0 208 166 137 846.0 0 137 167 132 846.0 0 132 168 223 846.0 0 223 169 228 846.0 0 228 170 223 846.0 0 223 171 274 846.0 0 274 172 373 846.0 0 373 173 1219 846.0 0 1219 174 1097 846.0 0 1097 175 4754 846.0 0 4754 176 8246 846.0 0 8246 177 13608 846.0 0 13608 178 141 846.0 0 141 179 90 846.0 0 90 180 73 846.0 0 73 181 259 846.0 0 259 182 2652 846.0 0 2652 183 113 846.0 0 113 184 58 846.0 0 58 185 59 846.0 0 59 186 80 846.0 0 80 187 52 846.0 0 52 188 34 846.0 0 34 189 70 846.0 0 70 190 64 846.0 0 64 191 56 846.0 0 56 192 34 846.0 0 34 193 53 846.0 0 53 194 37 846.0 0 37 195 57 846.0 0 57 196 78 846.0 0 78 197 64 846.0 0 64 198 48 846.0 0 48 199 38 846.0 0 38 200 32 846.0 0 32 201 66 846.0 0 66 202 72 846.0 0 72 203 64 846.0 0 64 204 72 846.0 0 72 205 76 846.0 0 76 206 83 846.0 0 83 207 77 846.0 0 77 208 95 846.0 0 95 209 128 846.0 0 128 210 168 846.0 0 168 211 270 846.0 0 270 212 474 846.0 0 474 213 17651 846.0 0 17651 214 833 846.0 0 833 215 6751 846.0 0 6751 216 126 846.0 0 126 217 64 846.0 0 64 218 86 846.0 0 86 219 45 846.0 0 45 220 43 846.0 0 43 221 61 846.0 0 61 222 49 846.0 0 49 223 51 846.0 0 51 224 52 846.0 0 52 225 42 846.0 0 42 226 57 846.0 0 57 227 73 846.0 0 73 228 356 846.0 0 356 229 94 846.0 0 94 230 81 846.0 0 81 231 175 846.0 0 175 232 209 846.0 0 209 233 150 846.0 0 150 234 76 846.0 0 76 235 85 846.0 0 85 236 51 846.0 0 51 237 44 846.0 0 44 238 58 846.0 0 58 239 49 846.0 0 49 240 37 846.0 0 37 241 55 846.0 0 55 242 46 846.0 0 46 243 70 846.0 0 70 244 101 846.0 0 101 245 57 846.0 0 57 246 50 846.0 0 50 247 62 846.0 0 62 248 51 846.0 0 51 249 60 846.0 0 60 250 88 846.0 0 88 251 199 846.0 0 199 252 240 846.0 0 240 253 690 846.0 0 690 254 302 846.0 0 302 255 420 846.0 0 420 256 335 846.0 0 335 257 134 846.0 0 134 258 85 846.0 0 85 259 47 846.0 0 47 260 20 846.0 0 20 261 41 846.0 0 41 262 23 846.0 0 23 263 36 846.0 0 36 264 45 846.0 0 45 265 64 846.0 0 64 266 53 846.0 0 53 267 33 846.0 0 33 268 22 846.0 0 22 269 29 846.0 0 29 270 50 846.0 0 50 271 77 846.0 0 77 272 47 846.0 0 47 273 29 846.0 0 29 274 34 846.0 0 34 275 46 846.0 0 46 276 54 846.0 0 54 277 47 846.0 0 47 278 60 846.0 0 60 279 49 846.0 0 49 280 35 846.0 0 35 281 36 846.0 0 36 282 38 846.0 0 38 283 99 846.0 0 99 284 55 846.0 0 55 285 54 846.0 0 54 286 78 846.0 0 78 287 85 846.0 0 85 288 46 846.0 0 46 289 41 846.0 0 41 290 58 846.0 0 58 291 119 846.0 0 119 292 80 846.0 0 80 293 107 846.0 0 107 294 166 846.0 0 166 295 97 846.0 0 97 296 109 846.0 0 109 297 364 846.0 0 364 298 952 846.0 0 952 299 471 846.0 0 471 300 92 846.0 0 92 301 41 846.0 0 41 Finished in 15.77 s (39 us/read; 1.56 M reads/minute). === Summary === Total reads processed: 408,749 Reads with adapters: 97,574 (23.9%) Reads that were too short: 2,081 (0.5%) Reads written (passing filters): 95,493 (23.4%) Total basepairs processed: 92,019,480 bp Total written (filtered): 25,838,271 bp (28.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 97574 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 14 0.0 3 0 0 6 8 16 389 0.0 3 0 347 25 17 17 91996 0.0 3 89001 2432 333 230 18 216 0.0 3 15 186 7 8 19 45 0.0 3 0 1 18 26 20 90 0.0 3 2 0 2 86 29 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 35 2 0.0 3 1 0 0 1 37 89 0.0 3 0 0 0 89 38 12 0.0 3 0 0 0 12 41 2 0.0 3 0 0 0 2 42 1 0.0 3 1 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 56 3 0.0 3 0 0 0 3 59 1 0.0 3 0 0 0 1 64 4 0.0 3 0 0 0 4 65 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 0 2 68 2 0.0 3 0 0 0 2 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 81 12 0.0 3 0 0 0 12 82 6 0.0 3 0 0 0 6 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 4 0.0 3 0 0 0 4 86 1377 0.0 3 0 0 9 1368 87 164 0.0 3 0 0 0 164 88 72 0.0 3 0 0 0 72 89 744 0.0 3 0 0 10 734 90 16 0.0 3 0 0 0 16 91 8 0.0 3 0 0 0 8 92 77 0.0 3 0 0 1 76 93 1 0.0 3 0 0 0 1 94 19 0.0 3 0 0 0 19 95 4 0.0 3 0 0 0 4 97 2 0.0 3 0 0 0 2 98 6 0.0 3 0 0 0 6 99 2 0.0 3 0 0 0 2 100 5 0.0 3 0 0 0 5 101 6 0.0 3 0 0 0 6 102 6 0.0 3 0 0 0 6 103 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 106 15 0.0 3 0 0 0 15 107 18 0.0 3 0 0 1 17 108 1 0.0 3 0 0 0 1 109 5 0.0 3 0 0 0 5 110 6 0.0 3 0 0 1 5 111 1 0.0 3 0 0 0 1 112 11 0.0 3 0 0 0 11 113 5 0.0 3 0 0 0 5 114 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 116 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 125 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 130 3 0.0 3 0 0 0 3 131 11 0.0 3 0 0 0 11 132 2 0.0 3 0 1 0 1 134 1 0.0 3 0 0 1 136 1 0.0 3 0 0 0 1 137 18 0.0 3 0 0 0 18 138 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 140 5 0.0 3 0 0 0 5 141 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 0 2 146 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 3 0.0 3 0 0 0 3 157 1 0.0 3 0 0 0 1 158 5 0.0 3 0 0 0 5 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 5 0.0 3 0 0 0 5 166 2 0.0 3 0 0 0 2 178 2 0.0 3 0 0 0 2 181 3 0.0 3 0 0 0 3 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 188 6 0.0 3 0 0 0 6 189 4 0.0 3 0 0 0 4 190 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 197 4 0.0 3 0 0 0 4 198 689 0.0 3 0 0 7 682 199 82 0.0 3 0 0 3 79 200 273 0.0 3 0 0 3 270 201 559 0.0 3 0 0 17 542 202 130 0.0 3 0 0 3 127 203 31 0.0 3 0 0 2 29 204 52 0.0 3 0 0 0 52 205 2 0.0 3 0 0 0 2 206 30 0.0 3 0 0 0 30 207 29 0.0 3 0 0 4 25 208 48 0.0 3 0 0 1 47 209 17 0.0 3 0 0 0 17 210 1 0.0 3 0 0 0 1 211 14 0.0 3 0 0 0 14 212 2 0.0 3 0 0 0 2 213 3 0.0 3 0 0 0 3 214 3 0.0 3 0 0 0 3 215 1 0.0 3 0 0 0 1 216 6 0.0 3 0 0 0 6 217 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 226 3 0.0 3 0 0 0 3 234 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 0 1 275 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.30 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 95,493 Reads with adapters: 4,041 (4.2%) Reads written (passing filters): 95,493 (100.0%) Total basepairs processed: 25,838,271 bp Total written (filtered): 25,826,048 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 4041 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 54.1% C: 10.0% G: 32.0% T: 3.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3972 1492.1 0 3972 4 66 373.0 0 66 5 2 93.3 0 2 33 1 0.0 2 0 0 1