This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CAGAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 89.78 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 137,320 (4.0%) Reads that were too short: 24,802 (0.7%) Reads written (passing filters): 112,518 (3.2%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 31,007,213 bp (3.0%) === Adapter 1 === Sequence: CAGAGC; Type: regular 5'; Length: 6; Trimmed: 137320 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1107 846.0 0 1107 7 93823 846.0 0 93823 8 99 846.0 0 99 9 47 846.0 0 47 10 6 846.0 0 6 11 424 846.0 0 424 12 14 846.0 0 14 13 4 846.0 0 4 14 3 846.0 0 3 15 4 846.0 0 4 16 5 846.0 0 5 17 1 846.0 0 1 18 7 846.0 0 7 19 7 846.0 0 7 20 5 846.0 0 5 21 6 846.0 0 6 22 5 846.0 0 5 23 7 846.0 0 7 24 5 846.0 0 5 25 5 846.0 0 5 26 5 846.0 0 5 27 38 846.0 0 38 28 21 846.0 0 21 29 55 846.0 0 55 30 4 846.0 0 4 31 6 846.0 0 6 32 5 846.0 0 5 33 5 846.0 0 5 34 3 846.0 0 3 35 4 846.0 0 4 36 8 846.0 0 8 37 6 846.0 0 6 38 100 846.0 0 100 39 3 846.0 0 3 40 4 846.0 0 4 41 2 846.0 0 2 42 5 846.0 0 5 43 12 846.0 0 12 44 10 846.0 0 10 45 8 846.0 0 8 46 9 846.0 0 9 47 7 846.0 0 7 48 16 846.0 0 16 49 17 846.0 0 17 50 9 846.0 0 9 51 23 846.0 0 23 52 24 846.0 0 24 53 10 846.0 0 10 54 30 846.0 0 30 55 40 846.0 0 40 56 895 846.0 0 895 57 19 846.0 0 19 58 171 846.0 0 171 59 39 846.0 0 39 60 16 846.0 0 16 61 21 846.0 0 21 62 62 846.0 0 62 63 29 846.0 0 29 64 196 846.0 0 196 65 12 846.0 0 12 66 46 846.0 0 46 67 114 846.0 0 114 68 162 846.0 0 162 69 8 846.0 0 8 70 7 846.0 0 7 71 15 846.0 0 15 72 13 846.0 0 13 73 24 846.0 0 24 74 24 846.0 0 24 75 25 846.0 0 25 76 24 846.0 0 24 77 34 846.0 0 34 78 836 846.0 0 836 79 195 846.0 0 195 80 715 846.0 0 715 81 601 846.0 0 601 82 140 846.0 0 140 83 338 846.0 0 338 84 55 846.0 0 55 85 15 846.0 0 15 86 8 846.0 0 8 87 12 846.0 0 12 88 8 846.0 0 8 89 25 846.0 0 25 90 20 846.0 0 20 91 21 846.0 0 21 92 39 846.0 0 39 93 26 846.0 0 26 94 46 846.0 0 46 95 42 846.0 0 42 96 42 846.0 0 42 97 25 846.0 0 25 98 44 846.0 0 44 99 39 846.0 0 39 100 43 846.0 0 43 101 47 846.0 0 47 102 20 846.0 0 20 103 23 846.0 0 23 104 6 846.0 0 6 105 12 846.0 0 12 106 18 846.0 0 18 107 16 846.0 0 16 108 38 846.0 0 38 109 48 846.0 0 48 110 147 846.0 0 147 111 30 846.0 0 30 112 29 846.0 0 29 113 26 846.0 0 26 114 60 846.0 0 60 115 99 846.0 0 99 116 359 846.0 0 359 117 292 846.0 0 292 118 142 846.0 0 142 119 43 846.0 0 43 120 57 846.0 0 57 121 79 846.0 0 79 122 92 846.0 0 92 123 94 846.0 0 94 124 53 846.0 0 53 125 48 846.0 0 48 126 67 846.0 0 67 127 49 846.0 0 49 128 69 846.0 0 69 129 91 846.0 0 91 130 88 846.0 0 88 131 85 846.0 0 85 132 53 846.0 0 53 133 75 846.0 0 75 134 59 846.0 0 59 135 92 846.0 0 92 136 95 846.0 0 95 137 112 846.0 0 112 138 129 846.0 0 129 139 80 846.0 0 80 140 95 846.0 0 95 141 63 846.0 0 63 142 195 846.0 0 195 143 104 846.0 0 104 144 79 846.0 0 79 145 87 846.0 0 87 146 82 846.0 0 82 147 77 846.0 0 77 148 92 846.0 0 92 149 66 846.0 0 66 150 85 846.0 0 85 151 97 846.0 0 97 152 98 846.0 0 98 153 154 846.0 0 154 154 80 846.0 0 80 155 152 846.0 0 152 156 116 846.0 0 116 157 149 846.0 0 149 158 137 846.0 0 137 159 92 846.0 0 92 160 172 846.0 0 172 161 84 846.0 0 84 162 122 846.0 0 122 163 113 846.0 0 113 164 127 846.0 0 127 165 134 846.0 0 134 166 134 846.0 0 134 167 186 846.0 0 186 168 136 846.0 0 136 169 113 846.0 0 113 170 133 846.0 0 133 171 141 846.0 0 141 172 133 846.0 0 133 173 105 846.0 0 105 174 72 846.0 0 72 175 107 846.0 0 107 176 121 846.0 0 121 177 125 846.0 0 125 178 124 846.0 0 124 179 139 846.0 0 139 180 123 846.0 0 123 181 163 846.0 0 163 182 228 846.0 0 228 183 291 846.0 0 291 184 122 846.0 0 122 185 190 846.0 0 190 186 156 846.0 0 156 187 239 846.0 0 239 188 158 846.0 0 158 189 117 846.0 0 117 190 154 846.0 0 154 191 157 846.0 0 157 192 213 846.0 0 213 193 124 846.0 0 124 194 111 846.0 0 111 195 145 846.0 0 145 196 162 846.0 0 162 197 212 846.0 0 212 198 164 846.0 0 164 199 91 846.0 0 91 200 117 846.0 0 117 201 74 846.0 0 74 202 114 846.0 0 114 203 113 846.0 0 113 204 203 846.0 0 203 205 235 846.0 0 235 206 146 846.0 0 146 207 99 846.0 0 99 208 141 846.0 0 141 209 150 846.0 0 150 210 163 846.0 0 163 211 210 846.0 0 210 212 163 846.0 0 163 213 186 846.0 0 186 214 214 846.0 0 214 215 137 846.0 0 137 216 250 846.0 0 250 217 261 846.0 0 261 218 363 846.0 0 363 219 274 846.0 0 274 220 261 846.0 0 261 221 242 846.0 0 242 222 209 846.0 0 209 223 197 846.0 0 197 224 171 846.0 0 171 225 874 846.0 0 874 226 457 846.0 0 457 227 796 846.0 0 796 228 316 846.0 0 316 229 199 846.0 0 199 230 190 846.0 0 190 231 109 846.0 0 109 232 148 846.0 0 148 233 224 846.0 0 224 234 168 846.0 0 168 235 178 846.0 0 178 236 225 846.0 0 225 237 380 846.0 0 380 238 444 846.0 0 444 239 245 846.0 0 245 240 171 846.0 0 171 241 158 846.0 0 158 242 120 846.0 0 120 243 166 846.0 0 166 244 158 846.0 0 158 245 249 846.0 0 249 246 197 846.0 0 197 247 151 846.0 0 151 248 122 846.0 0 122 249 155 846.0 0 155 250 268 846.0 0 268 251 122 846.0 0 122 252 146 846.0 0 146 253 122 846.0 0 122 254 203 846.0 0 203 255 191 846.0 0 191 256 180 846.0 0 180 257 146 846.0 0 146 258 121 846.0 0 121 259 145 846.0 0 145 260 538 846.0 0 538 261 421 846.0 0 421 262 937 846.0 0 937 263 435 846.0 0 435 264 472 846.0 0 472 265 511 846.0 0 511 266 231 846.0 0 231 267 225 846.0 0 225 268 162 846.0 0 162 269 206 846.0 0 206 270 286 846.0 0 286 271 182 846.0 0 182 272 249 846.0 0 249 273 198 846.0 0 198 274 172 846.0 0 172 275 151 846.0 0 151 276 185 846.0 0 185 277 242 846.0 0 242 278 218 846.0 0 218 279 151 846.0 0 151 280 154 846.0 0 154 281 276 846.0 0 276 282 191 846.0 0 191 283 241 846.0 0 241 284 131 846.0 0 131 285 382 846.0 0 382 286 564 846.0 0 564 287 335 846.0 0 335 288 315 846.0 0 315 289 266 846.0 0 266 290 524 846.0 0 524 291 323 846.0 0 323 292 183 846.0 0 183 293 123 846.0 0 123 294 190 846.0 0 190 295 171 846.0 0 171 296 369 846.0 0 369 297 321 846.0 0 321 298 336 846.0 0 336 299 245 846.0 0 245 300 178 846.0 0 178 301 66 846.0 0 66 Finished in 5.06 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 112,518 Reads with adapters: 94,971 (84.4%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 94,965 (84.4%) Total basepairs processed: 31,007,213 bp Total written (filtered): 26,019,699 bp (83.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 94971 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 2 0.0 3 0 0 0 2 18 9 0.0 3 0 0 8 0 1 19 345 0.0 3 0 331 14 20 94170 0.0 4 93107 1020 32 9 2 21 239 0.0 4 35 200 3 0 1 22 28 0.0 4 2 3 22 1 23 154 0.0 4 129 1 1 19 4 24 3 0.0 4 0 0 0 0 3 30 2 0.0 4 1 0 0 0 1 38 1 0.0 4 1 39 1 0.0 4 1 53 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 68 6 0.0 4 0 0 0 1 5 69 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 128 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.23 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 94,965 Reads with adapters: 1,917 (2.0%) Reads written (passing filters): 94,965 (100.0%) Total basepairs processed: 26,019,699 bp Total written (filtered): 25,968,476 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1917 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.8% C: 2.1% G: 94.4% T: 0.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 24 1483.8 0 24 4 3 371.0 0 3 5 3 92.7 0 3 10 6 0.1 1 0 6 11 1486 0.0 1 5 1481 12 60 0.0 1 25 35 13 204 0.0 1 166 38 14 4 0.0 1 1 3 152 20 0.0 1 3 17 205 2 0.0 1 1 1 210 3 0.0 1 0 3 230 1 0.0 1 1 257 13 0.0 1 11 2 261 1 0.0 1 1 264 1 0.0 1 1 267 20 0.0 1 19 1 269 58 0.0 1 56 2 270 2 0.0 1 2 272 6 0.0 1 6 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.