This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 88.94 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 46,449 (1.3%) Reads that were too short: 9,483 (0.3%) Reads written (passing filters): 36,966 (1.1%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 9,455,928 bp (0.9%) === Adapter 1 === Sequence: GTGATC; Type: regular 5'; Length: 6; Trimmed: 46449 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 134 846.0 0 134 7 560 846.0 0 560 8 8667 846.0 0 8667 9 15040 846.0 0 15040 10 104 846.0 0 104 11 7 846.0 0 7 12 7 846.0 0 7 13 11 846.0 0 11 14 12 846.0 0 12 15 10 846.0 0 10 16 6 846.0 0 6 17 7 846.0 0 7 18 4 846.0 0 4 19 7 846.0 0 7 20 12 846.0 0 12 21 8 846.0 0 8 22 15 846.0 0 15 23 16 846.0 0 16 24 6 846.0 0 6 25 5 846.0 0 5 26 2 846.0 0 2 27 11 846.0 0 11 28 6 846.0 0 6 29 18 846.0 0 18 30 13 846.0 0 13 31 11 846.0 0 11 32 6 846.0 0 6 33 77 846.0 0 77 34 12 846.0 0 12 35 23 846.0 0 23 36 183 846.0 0 183 37 11 846.0 0 11 38 39 846.0 0 39 39 13 846.0 0 13 40 9 846.0 0 9 41 9 846.0 0 9 42 8 846.0 0 8 43 6 846.0 0 6 44 13 846.0 0 13 45 83 846.0 0 83 46 19 846.0 0 19 47 7 846.0 0 7 48 7 846.0 0 7 49 12 846.0 0 12 50 20 846.0 0 20 51 10 846.0 0 10 52 7 846.0 0 7 53 13 846.0 0 13 54 10 846.0 0 10 55 7 846.0 0 7 56 10 846.0 0 10 57 18 846.0 0 18 58 124 846.0 0 124 59 68 846.0 0 68 60 155 846.0 0 155 61 10 846.0 0 10 62 31 846.0 0 31 63 23 846.0 0 23 64 9 846.0 0 9 65 10 846.0 0 10 66 10 846.0 0 10 67 21 846.0 0 21 68 11 846.0 0 11 69 13 846.0 0 13 70 24 846.0 0 24 71 9 846.0 0 9 72 11 846.0 0 11 73 18 846.0 0 18 74 17 846.0 0 17 75 62 846.0 0 62 76 1773 846.0 0 1773 77 46 846.0 0 46 78 245 846.0 0 245 79 20 846.0 0 20 80 327 846.0 0 327 81 415 846.0 0 415 82 83 846.0 0 83 83 27 846.0 0 27 84 19 846.0 0 19 85 19 846.0 0 19 86 15 846.0 0 15 87 65 846.0 0 65 88 28 846.0 0 28 89 65 846.0 0 65 90 43 846.0 0 43 91 39 846.0 0 39 92 15 846.0 0 15 93 39 846.0 0 39 94 42 846.0 0 42 95 66 846.0 0 66 96 46 846.0 0 46 97 62 846.0 0 62 98 98 846.0 0 98 99 46 846.0 0 46 100 321 846.0 0 321 101 804 846.0 0 804 102 275 846.0 0 275 103 25 846.0 0 25 104 7 846.0 0 7 105 33 846.0 0 33 106 19 846.0 0 19 107 9 846.0 0 9 108 18 846.0 0 18 109 71 846.0 0 71 110 30 846.0 0 30 111 56 846.0 0 56 112 257 846.0 0 257 113 84 846.0 0 84 114 20 846.0 0 20 115 21 846.0 0 21 116 29 846.0 0 29 117 33 846.0 0 33 118 31 846.0 0 31 119 33 846.0 0 33 120 22 846.0 0 22 121 24 846.0 0 24 122 28 846.0 0 28 123 35 846.0 0 35 124 29 846.0 0 29 125 26 846.0 0 26 126 25 846.0 0 25 127 53 846.0 0 53 128 25 846.0 0 25 129 27 846.0 0 27 130 43 846.0 0 43 131 46 846.0 0 46 132 61 846.0 0 61 133 59 846.0 0 59 134 54 846.0 0 54 135 29 846.0 0 29 136 38 846.0 0 38 137 37 846.0 0 37 138 76 846.0 0 76 139 39 846.0 0 39 140 31 846.0 0 31 141 50 846.0 0 50 142 48 846.0 0 48 143 49 846.0 0 49 144 48 846.0 0 48 145 40 846.0 0 40 146 30 846.0 0 30 147 148 846.0 0 148 148 41 846.0 0 41 149 64 846.0 0 64 150 55 846.0 0 55 151 55 846.0 0 55 152 25 846.0 0 25 153 35 846.0 0 35 154 40 846.0 0 40 155 66 846.0 0 66 156 64 846.0 0 64 157 64 846.0 0 64 158 79 846.0 0 79 159 62 846.0 0 62 160 85 846.0 0 85 161 62 846.0 0 62 162 121 846.0 0 121 163 62 846.0 0 62 164 55 846.0 0 55 165 42 846.0 0 42 166 41 846.0 0 41 167 62 846.0 0 62 168 162 846.0 0 162 169 139 846.0 0 139 170 179 846.0 0 179 171 41 846.0 0 41 172 39 846.0 0 39 173 34 846.0 0 34 174 42 846.0 0 42 175 50 846.0 0 50 176 60 846.0 0 60 177 96 846.0 0 96 178 72 846.0 0 72 179 56 846.0 0 56 180 49 846.0 0 49 181 50 846.0 0 50 182 60 846.0 0 60 183 66 846.0 0 66 184 84 846.0 0 84 185 63 846.0 0 63 186 78 846.0 0 78 187 99 846.0 0 99 188 71 846.0 0 71 189 67 846.0 0 67 190 25 846.0 0 25 191 56 846.0 0 56 192 68 846.0 0 68 193 54 846.0 0 54 194 73 846.0 0 73 195 137 846.0 0 137 196 69 846.0 0 69 197 100 846.0 0 100 198 144 846.0 0 144 199 109 846.0 0 109 200 193 846.0 0 193 201 105 846.0 0 105 202 97 846.0 0 97 203 58 846.0 0 58 204 239 846.0 0 239 205 116 846.0 0 116 206 100 846.0 0 100 207 109 846.0 0 109 208 89 846.0 0 89 209 88 846.0 0 88 210 62 846.0 0 62 211 73 846.0 0 73 212 101 846.0 0 101 213 114 846.0 0 114 214 111 846.0 0 111 215 93 846.0 0 93 216 114 846.0 0 114 217 123 846.0 0 123 218 119 846.0 0 119 219 117 846.0 0 117 220 108 846.0 0 108 221 121 846.0 0 121 222 203 846.0 0 203 223 181 846.0 0 181 224 339 846.0 0 339 225 114 846.0 0 114 226 87 846.0 0 87 227 82 846.0 0 82 228 274 846.0 0 274 229 96 846.0 0 96 230 77 846.0 0 77 231 58 846.0 0 58 232 67 846.0 0 67 233 64 846.0 0 64 234 73 846.0 0 73 235 62 846.0 0 62 236 83 846.0 0 83 237 187 846.0 0 187 238 82 846.0 0 82 239 85 846.0 0 85 240 214 846.0 0 214 241 196 846.0 0 196 242 179 846.0 0 179 243 93 846.0 0 93 244 91 846.0 0 91 245 95 846.0 0 95 246 53 846.0 0 53 247 70 846.0 0 70 248 82 846.0 0 82 249 89 846.0 0 89 250 61 846.0 0 61 251 49 846.0 0 49 252 28 846.0 0 28 253 73 846.0 0 73 254 100 846.0 0 100 255 122 846.0 0 122 256 120 846.0 0 120 257 96 846.0 0 96 258 105 846.0 0 105 259 98 846.0 0 98 260 82 846.0 0 82 261 40 846.0 0 40 262 73 846.0 0 73 263 54 846.0 0 54 264 68 846.0 0 68 265 67 846.0 0 67 266 64 846.0 0 64 267 62 846.0 0 62 268 81 846.0 0 81 269 101 846.0 0 101 270 74 846.0 0 74 271 49 846.0 0 49 272 75 846.0 0 75 273 45 846.0 0 45 274 41 846.0 0 41 275 71 846.0 0 71 276 115 846.0 0 115 277 61 846.0 0 61 278 34 846.0 0 34 279 42 846.0 0 42 280 83 846.0 0 83 281 146 846.0 0 146 282 78 846.0 0 78 283 45 846.0 0 45 284 73 846.0 0 73 285 51 846.0 0 51 286 41 846.0 0 41 287 90 846.0 0 90 288 86 846.0 0 86 289 82 846.0 0 82 290 64 846.0 0 64 291 39 846.0 0 39 292 81 846.0 0 81 293 252 846.0 0 252 294 262 846.0 0 262 295 82 846.0 0 82 296 55 846.0 0 55 297 38 846.0 0 38 298 50 846.0 0 50 299 37 846.0 0 37 300 31 846.0 0 31 301 38 846.0 0 38 Finished in 1.65 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 36,966 Reads with adapters: 8,689 (23.5%) Reads that were too short: 27 (0.1%) Reads written (passing filters): 8,662 (23.4%) Total basepairs processed: 9,455,928 bp Total written (filtered): 2,388,409 bp (25.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 8689 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 4 0.0 3 0 0 3 1 16 56 0.0 3 0 51 3 2 17 8549 0.0 3 8272 252 23 2 18 22 0.0 3 1 18 3 19 10 0.0 3 0 1 7 2 20 5 0.0 3 0 0 0 5 22 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 161 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 175 2 0.0 3 0 0 0 2 186 2 0.0 3 0 0 0 2 188 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 193 1 0.0 3 0 0 0 1 195 3 0.0 3 0 0 0 3 208 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 282 2 0.0 3 0 0 0 2 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.30 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 8,662 Reads with adapters: 58 (0.7%) Reads written (passing filters): 8,662 (100.0%) Total basepairs processed: 2,388,409 bp Total written (filtered): 2,388,231 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 58 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 36.2% C: 31.0% G: 12.1% T: 20.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 57 135.3 0 57 7 1 0.5 0 1