This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATTCTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 91.13 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 353,366 (10.2%) Reads that were too short: 25,174 (0.7%) Reads written (passing filters): 328,192 (9.5%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 54,478,444 bp (5.2%) === Adapter 1 === Sequence: ATTCTA; Type: regular 5'; Length: 6; Trimmed: 353366 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 160 846.0 0 160 7 9397 846.0 0 9397 8 8468 846.0 0 8468 9 24 846.0 0 24 10 10 846.0 0 10 11 22 846.0 0 22 12 30 846.0 0 30 13 72 846.0 0 72 14 12 846.0 0 12 15 9 846.0 0 9 16 9 846.0 0 9 17 12 846.0 0 12 18 12 846.0 0 12 19 8 846.0 0 8 20 17 846.0 0 17 21 10 846.0 0 10 22 3 846.0 0 3 23 8 846.0 0 8 24 9 846.0 0 9 25 11 846.0 0 11 26 9 846.0 0 9 27 22 846.0 0 22 28 19 846.0 0 19 29 6 846.0 0 6 30 9 846.0 0 9 31 11 846.0 0 11 32 25 846.0 0 25 33 7 846.0 0 7 34 23 846.0 0 23 35 42 846.0 0 42 36 13 846.0 0 13 37 5 846.0 0 5 38 163 846.0 0 163 39 15 846.0 0 15 40 8 846.0 0 8 41 3 846.0 0 3 42 9 846.0 0 9 43 19 846.0 0 19 44 9 846.0 0 9 45 3 846.0 0 3 46 7 846.0 0 7 47 11 846.0 0 11 48 12 846.0 0 12 49 12 846.0 0 12 50 6 846.0 0 6 51 15 846.0 0 15 52 2 846.0 0 2 53 6 846.0 0 6 54 12 846.0 0 12 55 14 846.0 0 14 56 16 846.0 0 16 57 10 846.0 0 10 58 11 846.0 0 11 59 19 846.0 0 19 60 12 846.0 0 12 61 5 846.0 0 5 62 13 846.0 0 13 63 10 846.0 0 10 64 15 846.0 0 15 65 9 846.0 0 9 66 18 846.0 0 18 67 10 846.0 0 10 68 10 846.0 0 10 69 11 846.0 0 11 70 16 846.0 0 16 71 34 846.0 0 34 72 64 846.0 0 64 73 35 846.0 0 35 74 32 846.0 0 32 75 85 846.0 0 85 76 68 846.0 0 68 77 97 846.0 0 97 78 107 846.0 0 107 79 50 846.0 0 50 80 189 846.0 0 189 81 155 846.0 0 155 82 147 846.0 0 147 83 210 846.0 0 210 84 147 846.0 0 147 85 160 846.0 0 160 86 90 846.0 0 90 87 93 846.0 0 93 88 94 846.0 0 94 89 365 846.0 0 365 90 472 846.0 0 472 91 432 846.0 0 432 92 356 846.0 0 356 93 494 846.0 0 494 94 110 846.0 0 110 95 189 846.0 0 189 96 185 846.0 0 185 97 250 846.0 0 250 98 319 846.0 0 319 99 231 846.0 0 231 100 244 846.0 0 244 101 504 846.0 0 504 102 785 846.0 0 785 103 739 846.0 0 739 104 394 846.0 0 394 105 165 846.0 0 165 106 149 846.0 0 149 107 99 846.0 0 99 108 131 846.0 0 131 109 420 846.0 0 420 110 442 846.0 0 442 111 523 846.0 0 523 112 351 846.0 0 351 113 402 846.0 0 402 114 385 846.0 0 385 115 441 846.0 0 441 116 221 846.0 0 221 117 142 846.0 0 142 118 103 846.0 0 103 119 100 846.0 0 100 120 117 846.0 0 117 121 123 846.0 0 123 122 396 846.0 0 396 123 589 846.0 0 589 124 377 846.0 0 377 125 618 846.0 0 618 126 233 846.0 0 233 127 375 846.0 0 375 128 347 846.0 0 347 129 412 846.0 0 412 130 360 846.0 0 360 131 284 846.0 0 284 132 346 846.0 0 346 133 241 846.0 0 241 134 255 846.0 0 255 135 376 846.0 0 376 136 503 846.0 0 503 137 937 846.0 0 937 138 925 846.0 0 925 139 1547 846.0 0 1547 140 2555 846.0 0 2555 141 2899 846.0 0 2899 142 52642 846.0 0 52642 143 182178 846.0 0 182178 144 23371 846.0 0 23371 145 373 846.0 0 373 146 283 846.0 0 283 147 484 846.0 0 484 148 408 846.0 0 408 149 307 846.0 0 307 150 249 846.0 0 249 151 231 846.0 0 231 152 186 846.0 0 186 153 201 846.0 0 201 154 132 846.0 0 132 155 536 846.0 0 536 156 367 846.0 0 367 157 329 846.0 0 329 158 289 846.0 0 289 159 212 846.0 0 212 160 327 846.0 0 327 161 323 846.0 0 323 162 3060 846.0 0 3060 163 6026 846.0 0 6026 164 1452 846.0 0 1452 165 189 846.0 0 189 166 157 846.0 0 157 167 310 846.0 0 310 168 542 846.0 0 542 169 305 846.0 0 305 170 207 846.0 0 207 171 144 846.0 0 144 172 93 846.0 0 93 173 138 846.0 0 138 174 99 846.0 0 99 175 218 846.0 0 218 176 448 846.0 0 448 177 452 846.0 0 452 178 423 846.0 0 423 179 185 846.0 0 185 180 131 846.0 0 131 181 145 846.0 0 145 182 122 846.0 0 122 183 214 846.0 0 214 184 297 846.0 0 297 185 220 846.0 0 220 186 188 846.0 0 188 187 143 846.0 0 143 188 269 846.0 0 269 189 269 846.0 0 269 190 279 846.0 0 279 191 187 846.0 0 187 192 108 846.0 0 108 193 193 846.0 0 193 194 154 846.0 0 154 195 184 846.0 0 184 196 225 846.0 0 225 197 267 846.0 0 267 198 486 846.0 0 486 199 157 846.0 0 157 200 150 846.0 0 150 201 108 846.0 0 108 202 181 846.0 0 181 203 379 846.0 0 379 204 318 846.0 0 318 205 250 846.0 0 250 206 329 846.0 0 329 207 279 846.0 0 279 208 217 846.0 0 217 209 297 846.0 0 297 210 353 846.0 0 353 211 353 846.0 0 353 212 347 846.0 0 347 213 277 846.0 0 277 214 385 846.0 0 385 215 328 846.0 0 328 216 330 846.0 0 330 217 304 846.0 0 304 218 240 846.0 0 240 219 213 846.0 0 213 220 124 846.0 0 124 221 147 846.0 0 147 222 266 846.0 0 266 223 423 846.0 0 423 224 579 846.0 0 579 225 257 846.0 0 257 226 248 846.0 0 248 227 290 846.0 0 290 228 217 846.0 0 217 229 178 846.0 0 178 230 184 846.0 0 184 231 127 846.0 0 127 232 286 846.0 0 286 233 316 846.0 0 316 234 408 846.0 0 408 235 379 846.0 0 379 236 242 846.0 0 242 237 238 846.0 0 238 238 177 846.0 0 177 239 189 846.0 0 189 240 206 846.0 0 206 241 144 846.0 0 144 242 169 846.0 0 169 243 342 846.0 0 342 244 406 846.0 0 406 245 437 846.0 0 437 246 391 846.0 0 391 247 208 846.0 0 208 248 128 846.0 0 128 249 54 846.0 0 54 250 85 846.0 0 85 251 118 846.0 0 118 252 261 846.0 0 261 253 362 846.0 0 362 254 225 846.0 0 225 255 332 846.0 0 332 256 448 846.0 0 448 257 378 846.0 0 378 258 340 846.0 0 340 259 284 846.0 0 284 260 186 846.0 0 186 261 162 846.0 0 162 262 517 846.0 0 517 263 360 846.0 0 360 264 334 846.0 0 334 265 262 846.0 0 262 266 322 846.0 0 322 267 165 846.0 0 165 268 253 846.0 0 253 269 138 846.0 0 138 270 156 846.0 0 156 271 185 846.0 0 185 272 256 846.0 0 256 273 338 846.0 0 338 274 168 846.0 0 168 275 170 846.0 0 170 276 117 846.0 0 117 277 112 846.0 0 112 278 240 846.0 0 240 279 349 846.0 0 349 280 222 846.0 0 222 281 109 846.0 0 109 282 238 846.0 0 238 283 213 846.0 0 213 284 302 846.0 0 302 285 340 846.0 0 340 286 214 846.0 0 214 287 211 846.0 0 211 288 79 846.0 0 79 289 81 846.0 0 81 290 99 846.0 0 99 291 171 846.0 0 171 292 363 846.0 0 363 293 297 846.0 0 297 294 181 846.0 0 181 295 236 846.0 0 236 296 216 846.0 0 216 297 162 846.0 0 162 298 174 846.0 0 174 299 173 846.0 0 173 300 170 846.0 0 170 301 160 846.0 0 160 Finished in 10.06 s (31 us/read; 1.96 M reads/minute). === Summary === Total reads processed: 328,192 Reads with adapters: 9,402 (2.9%) Reads that were too short: 56 (0.0%) Reads written (passing filters): 9,346 (2.8%) Total basepairs processed: 54,478,444 bp Total written (filtered): 2,586,334 bp (4.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 9402 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 5 0.0 3 0 0 0 5 16 118 0.0 3 0 102 7 9 17 9149 0.0 3 8726 334 69 20 18 33 0.0 3 3 24 3 3 19 20 0.0 3 0 0 11 9 20 7 0.0 3 0 0 0 7 21 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 0 2 40 1 0.0 3 0 0 1 44 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 61 2 0.0 3 0 0 0 2 68 2 0.0 3 0 0 0 2 70 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 80 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 146 12 0.0 3 0 0 0 12 147 9 0.0 3 0 0 0 9 148 3 0.0 3 0 0 0 3 153 3 0.0 3 0 0 0 3 154 4 0.0 3 0 0 0 4 155 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 1 193 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 284 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.34 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 9,346 Reads with adapters: 807 (8.6%) Reads written (passing filters): 9,346 (100.0%) Total basepairs processed: 2,586,334 bp Total written (filtered): 2,583,913 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 807 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 27.6% C: 61.6% G: 5.8% T: 5.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 807 146.0 0 807