This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 87.27 s (25 us/read; 2.38 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 165,064 (4.8%) Reads that were too short: 132,471 (3.8%) Reads written (passing filters): 32,593 (0.9%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 7,311,347 bp (0.7%) === Adapter 1 === Sequence: GTGTAG; Type: regular 5'; Length: 6; Trimmed: 165064 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 103 846.0 0 103 7 436 846.0 0 436 8 10832 846.0 0 10832 9 66 846.0 0 66 10 35 846.0 0 35 11 50 846.0 0 50 12 33 846.0 0 33 13 40 846.0 0 40 14 32 846.0 0 32 15 32 846.0 0 32 16 28 846.0 0 28 17 33 846.0 0 33 18 38 846.0 0 38 19 31 846.0 0 31 20 29 846.0 0 29 21 79 846.0 0 79 22 64 846.0 0 64 23 52 846.0 0 52 24 32 846.0 0 32 25 36 846.0 0 36 26 31 846.0 0 31 27 28 846.0 0 28 28 34 846.0 0 34 29 26 846.0 0 26 30 35 846.0 0 35 31 46 846.0 0 46 32 47 846.0 0 47 33 37 846.0 0 37 34 35 846.0 0 35 35 26 846.0 0 26 36 34 846.0 0 34 37 33 846.0 0 33 38 45 846.0 0 45 39 53 846.0 0 53 40 46 846.0 0 46 41 42 846.0 0 42 42 313 846.0 0 313 43 534 846.0 0 534 44 269 846.0 0 269 45 50 846.0 0 50 46 123 846.0 0 123 47 53 846.0 0 53 48 95 846.0 0 95 49 95 846.0 0 95 50 215 846.0 0 215 51 248 846.0 0 248 52 66 846.0 0 66 53 395 846.0 0 395 54 49 846.0 0 49 55 64 846.0 0 64 56 128 846.0 0 128 57 64 846.0 0 64 58 166 846.0 0 166 59 39 846.0 0 39 60 46 846.0 0 46 61 50 846.0 0 50 62 34 846.0 0 34 63 109 846.0 0 109 64 49 846.0 0 49 65 47 846.0 0 47 66 53 846.0 0 53 67 70 846.0 0 70 68 50 846.0 0 50 69 71 846.0 0 71 70 46 846.0 0 46 71 65 846.0 0 65 72 40 846.0 0 40 73 50 846.0 0 50 74 52 846.0 0 52 75 312 846.0 0 312 76 489 846.0 0 489 77 422 846.0 0 422 78 384 846.0 0 384 79 72 846.0 0 72 80 122 846.0 0 122 81 45 846.0 0 45 82 56 846.0 0 56 83 56 846.0 0 56 84 70 846.0 0 70 85 44 846.0 0 44 86 52 846.0 0 52 87 40 846.0 0 40 88 49 846.0 0 49 89 43 846.0 0 43 90 39 846.0 0 39 91 59 846.0 0 59 92 50 846.0 0 50 93 37 846.0 0 37 94 52 846.0 0 52 95 49 846.0 0 49 96 69 846.0 0 69 97 100 846.0 0 100 98 62 846.0 0 62 99 35 846.0 0 35 100 77 846.0 0 77 101 52 846.0 0 52 102 40 846.0 0 40 103 46 846.0 0 46 104 59 846.0 0 59 105 53 846.0 0 53 106 40 846.0 0 40 107 44 846.0 0 44 108 46 846.0 0 46 109 41 846.0 0 41 110 45 846.0 0 45 111 41 846.0 0 41 112 56 846.0 0 56 113 48 846.0 0 48 114 26 846.0 0 26 115 46 846.0 0 46 116 41 846.0 0 41 117 52 846.0 0 52 118 55 846.0 0 55 119 53 846.0 0 53 120 63 846.0 0 63 121 58 846.0 0 58 122 60 846.0 0 60 123 77 846.0 0 77 124 53 846.0 0 53 125 68 846.0 0 68 126 95 846.0 0 95 127 76 846.0 0 76 128 64 846.0 0 64 129 121 846.0 0 121 130 317 846.0 0 317 131 223 846.0 0 223 132 446 846.0 0 446 133 107 846.0 0 107 134 115 846.0 0 115 135 104 846.0 0 104 136 1728 846.0 0 1728 137 2254 846.0 0 2254 138 1695 846.0 0 1695 139 100 846.0 0 100 140 51 846.0 0 51 141 44 846.0 0 44 142 54 846.0 0 54 143 44 846.0 0 44 144 47 846.0 0 47 145 54 846.0 0 54 146 51 846.0 0 51 147 53 846.0 0 53 148 67 846.0 0 67 149 59 846.0 0 59 150 52 846.0 0 52 151 46 846.0 0 46 152 46 846.0 0 46 153 51 846.0 0 51 154 49 846.0 0 49 155 41 846.0 0 41 156 40 846.0 0 40 157 42 846.0 0 42 158 41 846.0 0 41 159 47 846.0 0 47 160 46 846.0 0 46 161 78 846.0 0 78 162 55 846.0 0 55 163 56 846.0 0 56 164 72 846.0 0 72 165 74 846.0 0 74 166 60 846.0 0 60 167 63 846.0 0 63 168 79 846.0 0 79 169 60 846.0 0 60 170 49 846.0 0 49 171 538 846.0 0 538 172 48 846.0 0 48 173 63 846.0 0 63 174 44 846.0 0 44 175 72 846.0 0 72 176 70 846.0 0 70 177 76 846.0 0 76 178 54 846.0 0 54 179 44 846.0 0 44 180 52 846.0 0 52 181 41 846.0 0 41 182 58 846.0 0 58 183 77 846.0 0 77 184 56 846.0 0 56 185 57 846.0 0 57 186 85 846.0 0 85 187 129 846.0 0 129 188 130 846.0 0 130 189 357 846.0 0 357 190 73 846.0 0 73 191 43 846.0 0 43 192 60 846.0 0 60 193 61 846.0 0 61 194 77 846.0 0 77 195 102 846.0 0 102 196 81 846.0 0 81 197 73 846.0 0 73 198 57 846.0 0 57 199 47 846.0 0 47 200 45 846.0 0 45 201 82 846.0 0 82 202 98 846.0 0 98 203 45 846.0 0 45 204 53 846.0 0 53 205 57 846.0 0 57 206 71 846.0 0 71 207 60 846.0 0 60 208 63 846.0 0 63 209 62 846.0 0 62 210 55 846.0 0 55 211 55 846.0 0 55 212 59 846.0 0 59 213 82 846.0 0 82 214 61 846.0 0 61 215 83 846.0 0 83 216 81 846.0 0 81 217 73 846.0 0 73 218 64 846.0 0 64 219 80 846.0 0 80 220 77 846.0 0 77 221 78 846.0 0 78 222 116 846.0 0 116 223 85 846.0 0 85 224 104 846.0 0 104 225 153 846.0 0 153 226 595 846.0 0 595 227 652 846.0 0 652 228 567 846.0 0 567 229 408 846.0 0 408 230 383 846.0 0 383 231 740 846.0 0 740 232 170 846.0 0 170 233 168 846.0 0 168 234 239 846.0 0 239 235 236 846.0 0 236 236 539 846.0 0 539 237 1092 846.0 0 1092 238 949 846.0 0 949 239 236 846.0 0 236 240 259 846.0 0 259 241 438 846.0 0 438 242 1066 846.0 0 1066 243 638 846.0 0 638 244 397 846.0 0 397 245 458 846.0 0 458 246 606 846.0 0 606 247 777 846.0 0 777 248 1199 846.0 0 1199 249 1035 846.0 0 1035 250 2452 846.0 0 2452 251 91546 846.0 0 91546 252 6019 846.0 0 6019 253 13392 846.0 0 13392 254 215 846.0 0 215 255 84 846.0 0 84 256 78 846.0 0 78 257 94 846.0 0 94 258 105 846.0 0 105 259 72 846.0 0 72 260 60 846.0 0 60 261 59 846.0 0 59 262 84 846.0 0 84 263 50 846.0 0 50 264 77 846.0 0 77 265 58 846.0 0 58 266 61 846.0 0 61 267 86 846.0 0 86 268 94 846.0 0 94 269 71 846.0 0 71 270 44 846.0 0 44 271 31 846.0 0 31 272 49 846.0 0 49 273 43 846.0 0 43 274 72 846.0 0 72 275 62 846.0 0 62 276 56 846.0 0 56 277 79 846.0 0 79 278 55 846.0 0 55 279 44 846.0 0 44 280 76 846.0 0 76 281 58 846.0 0 58 282 57 846.0 0 57 283 68 846.0 0 68 284 73 846.0 0 73 285 108 846.0 0 108 286 86 846.0 0 86 287 73 846.0 0 73 288 90 846.0 0 90 289 52 846.0 0 52 290 54 846.0 0 54 291 54 846.0 0 54 292 68 846.0 0 68 293 81 846.0 0 81 294 88 846.0 0 88 295 65 846.0 0 65 296 61 846.0 0 61 297 77 846.0 0 77 298 56 846.0 0 56 299 99 846.0 0 99 300 49 846.0 0 49 301 24 846.0 0 24 Finished in 1.47 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 32,593 Reads with adapters: 11,327 (34.8%) Reads that were too short: 30 (0.1%) Reads written (passing filters): 11,297 (34.7%) Total basepairs processed: 7,311,347 bp Total written (filtered): 3,083,350 bp (42.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 11327 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 1 1 19 45 0.0 3 0 45 20 11201 0.0 4 11094 101 3 1 2 21 30 0.0 4 3 27 22 1 0.0 4 0 0 1 23 5 0.0 4 0 0 0 3 2 24 2 0.0 4 0 0 0 0 2 60 26 0.0 4 0 0 0 0 26 73 1 0.0 4 0 0 0 0 1 99 4 0.0 4 0 0 0 0 4 100 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.38 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 11,297 Reads with adapters: 384 (3.4%) Reads written (passing filters): 11,297 (100.0%) Total basepairs processed: 3,083,350 bp Total written (filtered): 3,070,453 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 384 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.3% C: 8.9% G: 88.8% T: 1.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 37 44.1 0 37 10 51 0.0 1 3 48 11 260 0.0 1 1 259 151 1 0.0 1 0 1 261 23 0.0 1 21 2 262 1 0.0 1 1 263 2 0.0 1 2 270 3 0.0 1 3 271 5 0.0 1 5 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.