This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGTAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 93.47 s (27 us/read; 2.22 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 231,537 (6.7%) Reads that were too short: 30,950 (0.9%) Reads written (passing filters): 200,587 (5.8%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 52,153,219 bp (5.0%) === Adapter 1 === Sequence: AGGTAA; Type: regular 5'; Length: 6; Trimmed: 231537 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1110 846.0 0 1110 7 72098 846.0 0 72098 8 866 846.0 0 866 9 68243 846.0 0 68243 10 360 846.0 0 360 11 49 846.0 0 49 12 69 846.0 0 69 13 50 846.0 0 50 14 37 846.0 0 37 15 45 846.0 0 45 16 53 846.0 0 53 17 43 846.0 0 43 18 37 846.0 0 37 19 45 846.0 0 45 20 31 846.0 0 31 21 62 846.0 0 62 22 62 846.0 0 62 23 60 846.0 0 60 24 245 846.0 0 245 25 101 846.0 0 101 26 53 846.0 0 53 27 69 846.0 0 69 28 74 846.0 0 74 29 142 846.0 0 142 30 94 846.0 0 94 31 339 846.0 0 339 32 114 846.0 0 114 33 490 846.0 0 490 34 133 846.0 0 133 35 6197 846.0 0 6197 36 54 846.0 0 54 37 61 846.0 0 61 38 28 846.0 0 28 39 44 846.0 0 44 40 49 846.0 0 49 41 37 846.0 0 37 42 38 846.0 0 38 43 39 846.0 0 39 44 39 846.0 0 39 45 39 846.0 0 39 46 47 846.0 0 47 47 33 846.0 0 33 48 53 846.0 0 53 49 40 846.0 0 40 50 33 846.0 0 33 51 67 846.0 0 67 52 51 846.0 0 51 53 43 846.0 0 43 54 46 846.0 0 46 55 309 846.0 0 309 56 75 846.0 0 75 57 51 846.0 0 51 58 49 846.0 0 49 59 67 846.0 0 67 60 122 846.0 0 122 61 43 846.0 0 43 62 52 846.0 0 52 63 55 846.0 0 55 64 557 846.0 0 557 65 88 846.0 0 88 66 52 846.0 0 52 67 113 846.0 0 113 68 362 846.0 0 362 69 71 846.0 0 71 70 242 846.0 0 242 71 36 846.0 0 36 72 41 846.0 0 41 73 61 846.0 0 61 74 105 846.0 0 105 75 138 846.0 0 138 76 107 846.0 0 107 77 86 846.0 0 86 78 287 846.0 0 287 79 536 846.0 0 536 80 350 846.0 0 350 81 298 846.0 0 298 82 224 846.0 0 224 83 204 846.0 0 204 84 232 846.0 0 232 85 463 846.0 0 463 86 509 846.0 0 509 87 524 846.0 0 524 88 614 846.0 0 614 89 319 846.0 0 319 90 138 846.0 0 138 91 191 846.0 0 191 92 228 846.0 0 228 93 210 846.0 0 210 94 527 846.0 0 527 95 1112 846.0 0 1112 96 808 846.0 0 808 97 297 846.0 0 297 98 345 846.0 0 345 99 407 846.0 0 407 100 376 846.0 0 376 101 504 846.0 0 504 102 322 846.0 0 322 103 211 846.0 0 211 104 163 846.0 0 163 105 83 846.0 0 83 106 33 846.0 0 33 107 51 846.0 0 51 108 68 846.0 0 68 109 95 846.0 0 95 110 137 846.0 0 137 111 168 846.0 0 168 112 170 846.0 0 170 113 188 846.0 0 188 114 162 846.0 0 162 115 157 846.0 0 157 116 703 846.0 0 703 117 527 846.0 0 527 118 368 846.0 0 368 119 233 846.0 0 233 120 286 846.0 0 286 121 541 846.0 0 541 122 301 846.0 0 301 123 263 846.0 0 263 124 211 846.0 0 211 125 325 846.0 0 325 126 365 846.0 0 365 127 368 846.0 0 368 128 214 846.0 0 214 129 206 846.0 0 206 130 408 846.0 0 408 131 358 846.0 0 358 132 522 846.0 0 522 133 678 846.0 0 678 134 475 846.0 0 475 135 735 846.0 0 735 136 430 846.0 0 430 137 443 846.0 0 443 138 332 846.0 0 332 139 450 846.0 0 450 140 348 846.0 0 348 141 240 846.0 0 240 142 259 846.0 0 259 143 180 846.0 0 180 144 243 846.0 0 243 145 315 846.0 0 315 146 304 846.0 0 304 147 250 846.0 0 250 148 233 846.0 0 233 149 264 846.0 0 264 150 191 846.0 0 191 151 231 846.0 0 231 152 232 846.0 0 232 153 282 846.0 0 282 154 227 846.0 0 227 155 348 846.0 0 348 156 405 846.0 0 405 157 396 846.0 0 396 158 351 846.0 0 351 159 251 846.0 0 251 160 247 846.0 0 247 161 259 846.0 0 259 162 186 846.0 0 186 163 170 846.0 0 170 164 199 846.0 0 199 165 223 846.0 0 223 166 294 846.0 0 294 167 363 846.0 0 363 168 282 846.0 0 282 169 239 846.0 0 239 170 261 846.0 0 261 171 236 846.0 0 236 172 199 846.0 0 199 173 226 846.0 0 226 174 337 846.0 0 337 175 1353 846.0 0 1353 176 376 846.0 0 376 177 385 846.0 0 385 178 293 846.0 0 293 179 342 846.0 0 342 180 1255 846.0 0 1255 181 598 846.0 0 598 182 792 846.0 0 792 183 387 846.0 0 387 184 331 846.0 0 331 185 434 846.0 0 434 186 336 846.0 0 336 187 373 846.0 0 373 188 258 846.0 0 258 189 133 846.0 0 133 190 215 846.0 0 215 191 300 846.0 0 300 192 312 846.0 0 312 193 494 846.0 0 494 194 784 846.0 0 784 195 710 846.0 0 710 196 669 846.0 0 669 197 381 846.0 0 381 198 515 846.0 0 515 199 527 846.0 0 527 200 1533 846.0 0 1533 201 683 846.0 0 683 202 1185 846.0 0 1185 203 1601 846.0 0 1601 204 233 846.0 0 233 205 259 846.0 0 259 206 284 846.0 0 284 207 273 846.0 0 273 208 220 846.0 0 220 209 166 846.0 0 166 210 200 846.0 0 200 211 242 846.0 0 242 212 305 846.0 0 305 213 304 846.0 0 304 214 265 846.0 0 265 215 251 846.0 0 251 216 191 846.0 0 191 217 187 846.0 0 187 218 235 846.0 0 235 219 278 846.0 0 278 220 226 846.0 0 226 221 277 846.0 0 277 222 202 846.0 0 202 223 209 846.0 0 209 224 237 846.0 0 237 225 191 846.0 0 191 226 224 846.0 0 224 227 189 846.0 0 189 228 306 846.0 0 306 229 213 846.0 0 213 230 184 846.0 0 184 231 187 846.0 0 187 232 147 846.0 0 147 233 153 846.0 0 153 234 179 846.0 0 179 235 249 846.0 0 249 236 241 846.0 0 241 237 181 846.0 0 181 238 224 846.0 0 224 239 276 846.0 0 276 240 289 846.0 0 289 241 143 846.0 0 143 242 224 846.0 0 224 243 168 846.0 0 168 244 153 846.0 0 153 245 162 846.0 0 162 246 228 846.0 0 228 247 283 846.0 0 283 248 317 846.0 0 317 249 1134 846.0 0 1134 250 112 846.0 0 112 251 166 846.0 0 166 252 176 846.0 0 176 253 238 846.0 0 238 254 252 846.0 0 252 255 243 846.0 0 243 256 259 846.0 0 259 257 342 846.0 0 342 258 501 846.0 0 501 259 319 846.0 0 319 260 355 846.0 0 355 261 175 846.0 0 175 262 215 846.0 0 215 263 394 846.0 0 394 264 342 846.0 0 342 265 362 846.0 0 362 266 480 846.0 0 480 267 523 846.0 0 523 268 335 846.0 0 335 269 203 846.0 0 203 270 113 846.0 0 113 271 178 846.0 0 178 272 179 846.0 0 179 273 256 846.0 0 256 274 1096 846.0 0 1096 275 434 846.0 0 434 276 277 846.0 0 277 277 297 846.0 0 297 278 669 846.0 0 669 279 239 846.0 0 239 280 247 846.0 0 247 281 111 846.0 0 111 282 93 846.0 0 93 283 87 846.0 0 87 284 129 846.0 0 129 285 155 846.0 0 155 286 241 846.0 0 241 287 208 846.0 0 208 288 203 846.0 0 203 289 134 846.0 0 134 290 559 846.0 0 559 291 324 846.0 0 324 292 1153 846.0 0 1153 293 367 846.0 0 367 294 427 846.0 0 427 295 272 846.0 0 272 296 244 846.0 0 244 297 411 846.0 0 411 298 312 846.0 0 312 299 465 846.0 0 465 300 453 846.0 0 453 301 550 846.0 0 550 Finished in 8.69 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 200,587 Reads with adapters: 75,423 (37.6%) Reads that were too short: 114 (0.1%) Reads written (passing filters): 75,309 (37.5%) Total basepairs processed: 52,153,219 bp Total written (filtered): 20,705,178 bp (39.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 75423 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 18 0.0 3 0 0 14 4 16 554 0.0 3 0 526 21 7 17 69591 0.0 3 67931 1444 167 49 18 4944 0.0 3 4682 225 27 10 19 40 0.0 3 1 10 15 14 20 56 0.0 3 0 1 3 52 21 29 0.0 3 17 0 0 12 23 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 1 29 1 0.0 3 0 0 0 1 30 2 0.0 3 0 0 0 2 31 1 0.0 3 1 32 1 0.0 3 1 33 1 0.0 3 0 0 0 1 36 8 0.0 3 0 0 2 6 38 1 0.0 3 0 0 0 1 39 4 0.0 3 0 0 0 4 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 57 6 0.0 3 0 0 0 6 58 2 0.0 3 0 0 0 2 60 4 0.0 3 0 0 0 4 61 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 0 2 71 2 0.0 3 0 0 1 1 74 1 0.0 3 1 75 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 93 4 0.0 3 0 0 0 4 96 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 103 14 0.0 3 0 0 0 14 105 5 0.0 3 0 0 0 5 106 10 0.0 3 0 0 0 10 111 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 151 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 159 2 0.0 3 0 0 0 2 160 11 0.0 3 0 0 0 11 161 3 0.0 3 0 0 0 3 163 5 0.0 3 0 0 1 4 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 1 175 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 208 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 218 3 0.0 3 0 0 0 3 225 1 0.0 3 0 0 0 1 270 3 0.0 3 0 0 0 3 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 6 0.0 3 0 0 0 6 281 9 0.0 3 0 0 0 9 282 13 0.0 3 0 0 0 13 283 4 0.0 3 0 0 1 3 286 1 0.0 3 0 0 0 1 288 6 0.0 3 0 0 0 6 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.63 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 75,309 Reads with adapters: 2,125 (2.8%) Reads written (passing filters): 75,309 (100.0%) Total basepairs processed: 20,705,178 bp Total written (filtered): 20,698,773 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2125 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 43.5% C: 7.2% G: 44.2% T: 5.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2097 1176.7 0 2097 4 27 294.2 0 27 6 1 18.4 0 1