This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 90.76 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 232,750 (6.7%) Reads that were too short: 47,038 (1.4%) Reads written (passing filters): 185,712 (5.4%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 46,321,458 bp (4.4%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 232750 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 758 846.0 0 758 7 9907 846.0 0 9907 8 415 846.0 0 415 9 4685 846.0 0 4685 10 784 846.0 0 784 11 971 846.0 0 971 12 21570 846.0 0 21570 13 32579 846.0 0 32579 14 53486 846.0 0 53486 15 310 846.0 0 310 16 29 846.0 0 29 17 38 846.0 0 38 18 30 846.0 0 30 19 27 846.0 0 27 20 26 846.0 0 26 21 24 846.0 0 24 22 32 846.0 0 32 23 40 846.0 0 40 24 68 846.0 0 68 25 37 846.0 0 37 26 55 846.0 0 55 27 113 846.0 0 113 28 29 846.0 0 29 29 28 846.0 0 28 30 36 846.0 0 36 31 25 846.0 0 25 32 30 846.0 0 30 33 39 846.0 0 39 34 32 846.0 0 32 35 39 846.0 0 39 36 49 846.0 0 49 37 77 846.0 0 77 38 2316 846.0 0 2316 39 45 846.0 0 45 40 28 846.0 0 28 41 21 846.0 0 21 42 21 846.0 0 21 43 64 846.0 0 64 44 64 846.0 0 64 45 29 846.0 0 29 46 21 846.0 0 21 47 14 846.0 0 14 48 16 846.0 0 16 49 17 846.0 0 17 50 22 846.0 0 22 51 21 846.0 0 21 52 21 846.0 0 21 53 27 846.0 0 27 54 23 846.0 0 23 55 21 846.0 0 21 56 30 846.0 0 30 57 18 846.0 0 18 58 28 846.0 0 28 59 19 846.0 0 19 60 23 846.0 0 23 61 23 846.0 0 23 62 24 846.0 0 24 63 26 846.0 0 26 64 27 846.0 0 27 65 37 846.0 0 37 66 24 846.0 0 24 67 36 846.0 0 36 68 38 846.0 0 38 69 22 846.0 0 22 70 30 846.0 0 30 71 48 846.0 0 48 72 70 846.0 0 70 73 199 846.0 0 199 74 108 846.0 0 108 75 98 846.0 0 98 76 98 846.0 0 98 77 125 846.0 0 125 78 124 846.0 0 124 79 119 846.0 0 119 80 65 846.0 0 65 81 86 846.0 0 86 82 104 846.0 0 104 83 141 846.0 0 141 84 133 846.0 0 133 85 105 846.0 0 105 86 168 846.0 0 168 87 190 846.0 0 190 88 233 846.0 0 233 89 273 846.0 0 273 90 331 846.0 0 331 91 306 846.0 0 306 92 262 846.0 0 262 93 261 846.0 0 261 94 483 846.0 0 483 95 406 846.0 0 406 96 471 846.0 0 471 97 378 846.0 0 378 98 159 846.0 0 159 99 119 846.0 0 119 100 105 846.0 0 105 101 109 846.0 0 109 102 111 846.0 0 111 103 121 846.0 0 121 104 3232 846.0 0 3232 105 874 846.0 0 874 106 667 846.0 0 667 107 295 846.0 0 295 108 891 846.0 0 891 109 558 846.0 0 558 110 408 846.0 0 408 111 340 846.0 0 340 112 373 846.0 0 373 113 345 846.0 0 345 114 240 846.0 0 240 115 178 846.0 0 178 116 158 846.0 0 158 117 125 846.0 0 125 118 119 846.0 0 119 119 237 846.0 0 237 120 576 846.0 0 576 121 473 846.0 0 473 122 260 846.0 0 260 123 162 846.0 0 162 124 278 846.0 0 278 125 419 846.0 0 419 126 1103 846.0 0 1103 127 2481 846.0 0 2481 128 2948 846.0 0 2948 129 613 846.0 0 613 130 195 846.0 0 195 131 148 846.0 0 148 132 166 846.0 0 166 133 292 846.0 0 292 134 375 846.0 0 375 135 693 846.0 0 693 136 543 846.0 0 543 137 286 846.0 0 286 138 676 846.0 0 676 139 753 846.0 0 753 140 1058 846.0 0 1058 141 518 846.0 0 518 142 433 846.0 0 433 143 229 846.0 0 229 144 280 846.0 0 280 145 413 846.0 0 413 146 619 846.0 0 619 147 538 846.0 0 538 148 363 846.0 0 363 149 299 846.0 0 299 150 222 846.0 0 222 151 788 846.0 0 788 152 1855 846.0 0 1855 153 864 846.0 0 864 154 577 846.0 0 577 155 405 846.0 0 405 156 338 846.0 0 338 157 264 846.0 0 264 158 680 846.0 0 680 159 1824 846.0 0 1824 160 615 846.0 0 615 161 674 846.0 0 674 162 376 846.0 0 376 163 303 846.0 0 303 164 299 846.0 0 299 165 406 846.0 0 406 166 384 846.0 0 384 167 353 846.0 0 353 168 226 846.0 0 226 169 239 846.0 0 239 170 257 846.0 0 257 171 198 846.0 0 198 172 277 846.0 0 277 173 394 846.0 0 394 174 631 846.0 0 631 175 452 846.0 0 452 176 357 846.0 0 357 177 265 846.0 0 265 178 217 846.0 0 217 179 260 846.0 0 260 180 323 846.0 0 323 181 601 846.0 0 601 182 436 846.0 0 436 183 356 846.0 0 356 184 264 846.0 0 264 185 374 846.0 0 374 186 421 846.0 0 421 187 366 846.0 0 366 188 357 846.0 0 357 189 223 846.0 0 223 190 358 846.0 0 358 191 412 846.0 0 412 192 451 846.0 0 451 193 327 846.0 0 327 194 530 846.0 0 530 195 220 846.0 0 220 196 211 846.0 0 211 197 226 846.0 0 226 198 377 846.0 0 377 199 366 846.0 0 366 200 292 846.0 0 292 201 318 846.0 0 318 202 408 846.0 0 408 203 604 846.0 0 604 204 398 846.0 0 398 205 259 846.0 0 259 206 381 846.0 0 381 207 591 846.0 0 591 208 510 846.0 0 510 209 351 846.0 0 351 210 274 846.0 0 274 211 236 846.0 0 236 212 242 846.0 0 242 213 316 846.0 0 316 214 434 846.0 0 434 215 470 846.0 0 470 216 421 846.0 0 421 217 316 846.0 0 316 218 309 846.0 0 309 219 363 846.0 0 363 220 702 846.0 0 702 221 474 846.0 0 474 222 350 846.0 0 350 223 266 846.0 0 266 224 592 846.0 0 592 225 372 846.0 0 372 226 721 846.0 0 721 227 269 846.0 0 269 228 392 846.0 0 392 229 312 846.0 0 312 230 353 846.0 0 353 231 394 846.0 0 394 232 354 846.0 0 354 233 468 846.0 0 468 234 509 846.0 0 509 235 403 846.0 0 403 236 421 846.0 0 421 237 236 846.0 0 236 238 228 846.0 0 228 239 274 846.0 0 274 240 513 846.0 0 513 241 454 846.0 0 454 242 573 846.0 0 573 243 637 846.0 0 637 244 446 846.0 0 446 245 326 846.0 0 326 246 258 846.0 0 258 247 353 846.0 0 353 248 189 846.0 0 189 249 223 846.0 0 223 250 107 846.0 0 107 251 434 846.0 0 434 252 614 846.0 0 614 253 722 846.0 0 722 254 372 846.0 0 372 255 387 846.0 0 387 256 388 846.0 0 388 257 446 846.0 0 446 258 335 846.0 0 335 259 184 846.0 0 184 260 219 846.0 0 219 261 371 846.0 0 371 262 471 846.0 0 471 263 440 846.0 0 440 264 343 846.0 0 343 265 269 846.0 0 269 266 349 846.0 0 349 267 935 846.0 0 935 268 748 846.0 0 748 269 580 846.0 0 580 270 273 846.0 0 273 271 385 846.0 0 385 272 356 846.0 0 356 273 298 846.0 0 298 274 414 846.0 0 414 275 370 846.0 0 370 276 315 846.0 0 315 277 635 846.0 0 635 278 899 846.0 0 899 279 1640 846.0 0 1640 280 871 846.0 0 871 281 1228 846.0 0 1228 282 1887 846.0 0 1887 283 868 846.0 0 868 284 651 846.0 0 651 285 326 846.0 0 326 286 387 846.0 0 387 287 396 846.0 0 396 288 2922 846.0 0 2922 289 387 846.0 0 387 290 291 846.0 0 291 291 299 846.0 0 299 292 446 846.0 0 446 293 444 846.0 0 444 294 668 846.0 0 668 295 421 846.0 0 421 296 308 846.0 0 308 297 194 846.0 0 194 298 163 846.0 0 163 299 193 846.0 0 193 300 257 846.0 0 257 301 187 846.0 0 187 Finished in 7.63 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 185,712 Reads with adapters: 10,618 (5.7%) Reads that were too short: 485 (0.3%) Reads written (passing filters): 10,133 (5.5%) Total basepairs processed: 46,321,458 bp Total written (filtered): 2,659,235 bp (5.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10618 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 16 49 0.0 3 0 44 4 1 17 8991 0.0 3 8793 165 25 8 18 28 0.0 3 1 21 2 4 19 10 0.0 3 0 0 7 3 20 93 0.0 3 78 1 0 14 22 1 0.0 3 1 27 1 0.0 3 1 31 2 0.0 3 0 0 0 2 32 5 0.0 3 0 0 0 5 33 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 35 3 0.0 3 1 0 0 2 37 2 0.0 3 1 1 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 43 4 0.0 3 0 0 0 4 45 1 0.0 3 0 0 0 1 53 7 0.0 3 0 0 0 7 56 2 0.0 3 0 0 1 1 57 3 0.0 3 0 0 0 3 58 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 67 55 0.0 3 0 0 0 55 69 2 0.0 3 0 0 0 2 70 13 0.0 3 0 0 0 13 71 1 0.0 3 0 0 0 1 72 3 0.0 3 0 0 0 3 73 1 0.0 3 0 0 0 1 75 2 0.0 3 0 0 0 2 76 2 0.0 3 0 0 0 2 77 3 0.0 3 1 0 0 2 78 1 0.0 3 0 0 0 1 79 1 0.0 3 1 81 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 88 2 0.0 3 0 0 0 2 89 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 110 25 0.0 3 0 0 0 25 111 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 124 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 138 17 0.0 3 0 0 0 17 141 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 154 30 0.0 3 0 0 0 30 155 8 0.0 3 0 0 0 8 156 2 0.0 3 0 0 0 2 157 7 0.0 3 0 0 0 7 158 5 0.0 3 0 0 0 5 159 201 0.0 3 0 0 2 199 160 58 0.0 3 0 0 0 58 161 28 0.0 3 0 0 0 28 162 22 0.0 3 0 0 1 21 163 8 0.0 3 0 0 0 8 164 1 0.0 3 0 0 0 1 165 10 0.0 3 0 0 0 10 168 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 2 0.0 3 0 0 0 2 173 1 0.0 3 0 0 0 1 174 6 0.0 3 0 0 0 6 175 2 0.0 3 0 0 0 2 176 1 0.0 3 0 0 0 1 177 2 0.0 3 0 0 0 2 178 7 0.0 3 0 0 0 7 179 255 0.0 3 0 0 0 255 180 75 0.0 3 0 0 0 75 181 15 0.0 3 0 0 0 15 182 58 0.0 3 0 0 0 58 183 9 0.0 3 0 0 0 9 185 5 0.0 3 0 0 0 5 186 1 0.0 3 0 0 0 1 187 3 0.0 3 0 0 0 3 188 1 0.0 3 0 0 0 1 189 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 205 3 0.0 3 0 0 0 3 207 1 0.0 3 0 0 0 1 210 2 0.0 3 0 0 0 2 214 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 230 3 0.0 3 0 0 0 3 232 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 239 2 0.0 3 0 0 0 2 240 2 0.0 3 0 0 0 2 246 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 261 3 0.0 3 0 0 0 3 266 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 0 1 269 2 0.0 3 0 0 0 2 270 1 0.0 3 0 0 0 1 271 178 0.0 3 0 0 2 176 272 35 0.0 3 0 0 1 34 273 3 0.0 3 0 0 0 3 274 75 0.0 3 0 0 4 71 275 29 0.0 3 0 0 0 29 276 5 0.0 3 0 0 0 5 277 4 0.0 3 0 0 0 4 279 4 0.0 3 0 0 0 4 280 17 0.0 3 0 0 0 17 281 36 0.0 3 0 0 1 35 282 2 0.0 3 0 0 0 2 283 2 0.0 3 0 0 0 2 287 2 0.0 3 0 0 0 2 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.36 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 10,133 Reads with adapters: 697 (6.9%) Reads written (passing filters): 10,133 (100.0%) Total basepairs processed: 2,659,235 bp Total written (filtered): 2,657,140 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 697 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 22.8% C: 59.3% G: 10.0% T: 7.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 695 158.3 0 695 5 2 9.9 0 2