This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 88.76 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 149,136 (4.3%) Reads that were too short: 65,553 (1.9%) Reads written (passing filters): 83,583 (2.4%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 22,057,075 bp (2.1%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 149136 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 337 846.0 0 337 7 718 846.0 0 718 8 30241 846.0 0 30241 9 30471 846.0 0 30471 10 57 846.0 0 57 11 16 846.0 0 16 12 8 846.0 0 8 13 12 846.0 0 12 14 18 846.0 0 18 15 15 846.0 0 15 16 17 846.0 0 17 17 16 846.0 0 16 18 21 846.0 0 21 19 50 846.0 0 50 20 21 846.0 0 21 21 38 846.0 0 38 22 44 846.0 0 44 23 21 846.0 0 21 24 16 846.0 0 16 25 22 846.0 0 22 26 24 846.0 0 24 27 20 846.0 0 20 28 22 846.0 0 22 29 40 846.0 0 40 30 16 846.0 0 16 31 13 846.0 0 13 32 28 846.0 0 28 33 65 846.0 0 65 34 23 846.0 0 23 35 20 846.0 0 20 36 214 846.0 0 214 37 19 846.0 0 19 38 36 846.0 0 36 39 14 846.0 0 14 40 14 846.0 0 14 41 9 846.0 0 9 42 16 846.0 0 16 43 13 846.0 0 13 44 9 846.0 0 9 45 12 846.0 0 12 46 10 846.0 0 10 47 20 846.0 0 20 48 14 846.0 0 14 49 16 846.0 0 16 50 20 846.0 0 20 51 44 846.0 0 44 52 16 846.0 0 16 53 15 846.0 0 15 54 44 846.0 0 44 55 23 846.0 0 23 56 45 846.0 0 45 57 147 846.0 0 147 58 35 846.0 0 35 59 50 846.0 0 50 60 25 846.0 0 25 61 40 846.0 0 40 62 23 846.0 0 23 63 16 846.0 0 16 64 32 846.0 0 32 65 22 846.0 0 22 66 11 846.0 0 11 67 28 846.0 0 28 68 13 846.0 0 13 69 77 846.0 0 77 70 111 846.0 0 111 71 49 846.0 0 49 72 29 846.0 0 29 73 39 846.0 0 39 74 40 846.0 0 40 75 72 846.0 0 72 76 70 846.0 0 70 77 26 846.0 0 26 78 50 846.0 0 50 79 45 846.0 0 45 80 72 846.0 0 72 81 79 846.0 0 79 82 59 846.0 0 59 83 83 846.0 0 83 84 51 846.0 0 51 85 95 846.0 0 95 86 88 846.0 0 88 87 132 846.0 0 132 88 232 846.0 0 232 89 4783 846.0 0 4783 90 1370 846.0 0 1370 91 1020 846.0 0 1020 92 39 846.0 0 39 93 77 846.0 0 77 94 82 846.0 0 82 95 67 846.0 0 67 96 170 846.0 0 170 97 200 846.0 0 200 98 250 846.0 0 250 99 141 846.0 0 141 100 127 846.0 0 127 101 169 846.0 0 169 102 110 846.0 0 110 103 93 846.0 0 93 104 32 846.0 0 32 105 27 846.0 0 27 106 16 846.0 0 16 107 20 846.0 0 20 108 37 846.0 0 37 109 32 846.0 0 32 110 29 846.0 0 29 111 55 846.0 0 55 112 45 846.0 0 45 113 31 846.0 0 31 114 46 846.0 0 46 115 47 846.0 0 47 116 19 846.0 0 19 117 29 846.0 0 29 118 39 846.0 0 39 119 39 846.0 0 39 120 46 846.0 0 46 121 47 846.0 0 47 122 35 846.0 0 35 123 31 846.0 0 31 124 43 846.0 0 43 125 39 846.0 0 39 126 69 846.0 0 69 127 53 846.0 0 53 128 48 846.0 0 48 129 51 846.0 0 51 130 45 846.0 0 45 131 56 846.0 0 56 132 73 846.0 0 73 133 205 846.0 0 205 134 70 846.0 0 70 135 63 846.0 0 63 136 47 846.0 0 47 137 64 846.0 0 64 138 50 846.0 0 50 139 118 846.0 0 118 140 91 846.0 0 91 141 68 846.0 0 68 142 38 846.0 0 38 143 41 846.0 0 41 144 50 846.0 0 50 145 83 846.0 0 83 146 96 846.0 0 96 147 100 846.0 0 100 148 455 846.0 0 455 149 282 846.0 0 282 150 362 846.0 0 362 151 52 846.0 0 52 152 28 846.0 0 28 153 56 846.0 0 56 154 67 846.0 0 67 155 48 846.0 0 48 156 73 846.0 0 73 157 99 846.0 0 99 158 76 846.0 0 76 159 78 846.0 0 78 160 62 846.0 0 62 161 75 846.0 0 75 162 61 846.0 0 61 163 51 846.0 0 51 164 64 846.0 0 64 165 63 846.0 0 63 166 63 846.0 0 63 167 50 846.0 0 50 168 516 846.0 0 516 169 198 846.0 0 198 170 401 846.0 0 401 171 33 846.0 0 33 172 30 846.0 0 30 173 63 846.0 0 63 174 69 846.0 0 69 175 75 846.0 0 75 176 943 846.0 0 943 177 1125 846.0 0 1125 178 509 846.0 0 509 179 61 846.0 0 61 180 46 846.0 0 46 181 48 846.0 0 48 182 77 846.0 0 77 183 51 846.0 0 51 184 72 846.0 0 72 185 61 846.0 0 61 186 90 846.0 0 90 187 43 846.0 0 43 188 46 846.0 0 46 189 39 846.0 0 39 190 39 846.0 0 39 191 41 846.0 0 41 192 50 846.0 0 50 193 90 846.0 0 90 194 106 846.0 0 106 195 99 846.0 0 99 196 72 846.0 0 72 197 38 846.0 0 38 198 57 846.0 0 57 199 40 846.0 0 40 200 51 846.0 0 51 201 84 846.0 0 84 202 68 846.0 0 68 203 88 846.0 0 88 204 98 846.0 0 98 205 144 846.0 0 144 206 81 846.0 0 81 207 286 846.0 0 286 208 397 846.0 0 397 209 251 846.0 0 251 210 131 846.0 0 131 211 66 846.0 0 66 212 94 846.0 0 94 213 92 846.0 0 92 214 177 846.0 0 177 215 163 846.0 0 163 216 126 846.0 0 126 217 130 846.0 0 130 218 165 846.0 0 165 219 282 846.0 0 282 220 3665 846.0 0 3665 221 3371 846.0 0 3371 222 2010 846.0 0 2010 223 81 846.0 0 81 224 72 846.0 0 72 225 82 846.0 0 82 226 88 846.0 0 88 227 73 846.0 0 73 228 93 846.0 0 93 229 47 846.0 0 47 230 108 846.0 0 108 231 106 846.0 0 106 232 71 846.0 0 71 233 94 846.0 0 94 234 96 846.0 0 96 235 117 846.0 0 117 236 71 846.0 0 71 237 72 846.0 0 72 238 133 846.0 0 133 239 157 846.0 0 157 240 132 846.0 0 132 241 147 846.0 0 147 242 136 846.0 0 136 243 116 846.0 0 116 244 169 846.0 0 169 245 145 846.0 0 145 246 199 846.0 0 199 247 73 846.0 0 73 248 73 846.0 0 73 249 46 846.0 0 46 250 30 846.0 0 30 251 67 846.0 0 67 252 52 846.0 0 52 253 81 846.0 0 81 254 136 846.0 0 136 255 221 846.0 0 221 256 416 846.0 0 416 257 386 846.0 0 386 258 103 846.0 0 103 259 87 846.0 0 87 260 75 846.0 0 75 261 65 846.0 0 65 262 50 846.0 0 50 263 63 846.0 0 63 264 85 846.0 0 85 265 125 846.0 0 125 266 199 846.0 0 199 267 128 846.0 0 128 268 167 846.0 0 167 269 437 846.0 0 437 270 508 846.0 0 508 271 364 846.0 0 364 272 217 846.0 0 217 273 217 846.0 0 217 274 363 846.0 0 363 275 500 846.0 0 500 276 860 846.0 0 860 277 76 846.0 0 76 278 106 846.0 0 106 279 251 846.0 0 251 280 447 846.0 0 447 281 720 846.0 0 720 282 795 846.0 0 795 283 14149 846.0 0 14149 284 15812 846.0 0 15812 285 9450 846.0 0 9450 286 680 846.0 0 680 287 68 846.0 0 68 288 683 846.0 0 683 289 75 846.0 0 75 290 165 846.0 0 165 291 65 846.0 0 65 292 79 846.0 0 79 293 96 846.0 0 96 294 132 846.0 0 132 295 104 846.0 0 104 296 723 846.0 0 723 297 41 846.0 0 41 298 51 846.0 0 51 299 45 846.0 0 45 300 21 846.0 0 21 301 35 846.0 0 35 Finished in 3.77 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 83,583 Reads with adapters: 30,784 (36.8%) Reads that were too short: 63 (0.1%) Reads written (passing filters): 30,721 (36.8%) Total basepairs processed: 22,057,075 bp Total written (filtered): 8,473,639 bp (38.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 30784 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 4 0.0 3 0 0 3 1 16 188 0.0 3 0 171 13 4 17 30330 0.0 3 29597 638 73 22 18 110 0.0 3 11 84 8 7 19 19 0.0 3 0 0 17 2 20 26 0.0 3 0 0 0 26 22 1 0.0 3 0 0 0 1 24 2 0.0 3 2 25 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 43 17 0.0 3 0 0 0 17 46 4 0.0 3 0 0 0 4 48 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 105 3 0.0 3 0 0 1 2 108 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 217 39 0.0 3 0 0 4 35 221 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 4 0.0 3 0 0 0 4 283 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.08 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 30,721 Reads with adapters: 759 (2.5%) Reads written (passing filters): 30,721 (100.0%) Total basepairs processed: 8,473,639 bp Total written (filtered): 8,471,313 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 759 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 58.8% C: 18.7% G: 15.8% T: 6.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 721 480.0 0 721 4 29 120.0 0 29 5 7 30.0 0 7 6 2 7.5 0 2