This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATCTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 90.30 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 404,706 (11.7%) Reads that were too short: 10,762 (0.3%) Reads written (passing filters): 393,944 (11.4%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 76,404,451 bp (7.3%) === Adapter 1 === Sequence: TATCTG; Type: regular 5'; Length: 6; Trimmed: 404706 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 195 846.0 0 195 7 204 846.0 0 204 8 334 846.0 0 334 9 29887 846.0 0 29887 10 78 846.0 0 78 11 45 846.0 0 45 12 30 846.0 0 30 13 30 846.0 0 30 14 54 846.0 0 54 15 41 846.0 0 41 16 34 846.0 0 34 17 43 846.0 0 43 18 53 846.0 0 53 19 49 846.0 0 49 20 34 846.0 0 34 21 29 846.0 0 29 22 48 846.0 0 48 23 33 846.0 0 33 24 39 846.0 0 39 25 78 846.0 0 78 26 107 846.0 0 107 27 47 846.0 0 47 28 36 846.0 0 36 29 36 846.0 0 36 30 36 846.0 0 36 31 36 846.0 0 36 32 68 846.0 0 68 33 42 846.0 0 42 34 31 846.0 0 31 35 33 846.0 0 33 36 48 846.0 0 48 37 38 846.0 0 38 38 37 846.0 0 37 39 44 846.0 0 44 40 32 846.0 0 32 41 42 846.0 0 42 42 41 846.0 0 41 43 55 846.0 0 55 44 35 846.0 0 35 45 47 846.0 0 47 46 35 846.0 0 35 47 31 846.0 0 31 48 37 846.0 0 37 49 35 846.0 0 35 50 25 846.0 0 25 51 37 846.0 0 37 52 49 846.0 0 49 53 49 846.0 0 49 54 30 846.0 0 30 55 45 846.0 0 45 56 134 846.0 0 134 57 45 846.0 0 45 58 46 846.0 0 46 59 34 846.0 0 34 60 62 846.0 0 62 61 45 846.0 0 45 62 33 846.0 0 33 63 33 846.0 0 33 64 46 846.0 0 46 65 38 846.0 0 38 66 44 846.0 0 44 67 64 846.0 0 64 68 49 846.0 0 49 69 37 846.0 0 37 70 544 846.0 0 544 71 185 846.0 0 185 72 282 846.0 0 282 73 35 846.0 0 35 74 31 846.0 0 31 75 24 846.0 0 24 76 73 846.0 0 73 77 80 846.0 0 80 78 51 846.0 0 51 79 53 846.0 0 53 80 53 846.0 0 53 81 77 846.0 0 77 82 267 846.0 0 267 83 248 846.0 0 248 84 397 846.0 0 397 85 77 846.0 0 77 86 446 846.0 0 446 87 49 846.0 0 49 88 32 846.0 0 32 89 30 846.0 0 30 90 44 846.0 0 44 91 60 846.0 0 60 92 88 846.0 0 88 93 150 846.0 0 150 94 114 846.0 0 114 95 272 846.0 0 272 96 594 846.0 0 594 97 147 846.0 0 147 98 201 846.0 0 201 99 108 846.0 0 108 100 200 846.0 0 200 101 266 846.0 0 266 102 202 846.0 0 202 103 197 846.0 0 197 104 97 846.0 0 97 105 166 846.0 0 166 106 224 846.0 0 224 107 373 846.0 0 373 108 841 846.0 0 841 109 1002 846.0 0 1002 110 1640 846.0 0 1640 111 2531 846.0 0 2531 112 3684 846.0 0 3684 113 145116 846.0 0 145116 114 128868 846.0 0 128868 115 32994 846.0 0 32994 116 763 846.0 0 763 117 308 846.0 0 308 118 297 846.0 0 297 119 66 846.0 0 66 120 95 846.0 0 95 121 215 846.0 0 215 122 374 846.0 0 374 123 89 846.0 0 89 124 145 846.0 0 145 125 193 846.0 0 193 126 130 846.0 0 130 127 137 846.0 0 137 128 198 846.0 0 198 129 220 846.0 0 220 130 162 846.0 0 162 131 150 846.0 0 150 132 228 846.0 0 228 133 247 846.0 0 247 134 269 846.0 0 269 135 1502 846.0 0 1502 136 7647 846.0 0 7647 137 17766 846.0 0 17766 138 178 846.0 0 178 139 101 846.0 0 101 140 179 846.0 0 179 141 147 846.0 0 147 142 56 846.0 0 56 143 79 846.0 0 79 144 85 846.0 0 85 145 80 846.0 0 80 146 107 846.0 0 107 147 150 846.0 0 150 148 80 846.0 0 80 149 59 846.0 0 59 150 62 846.0 0 62 151 58 846.0 0 58 152 88 846.0 0 88 153 88 846.0 0 88 154 80 846.0 0 80 155 108 846.0 0 108 156 82 846.0 0 82 157 90 846.0 0 90 158 260 846.0 0 260 159 292 846.0 0 292 160 238 846.0 0 238 161 59 846.0 0 59 162 54 846.0 0 54 163 63 846.0 0 63 164 109 846.0 0 109 165 73 846.0 0 73 166 70 846.0 0 70 167 96 846.0 0 96 168 107 846.0 0 107 169 86 846.0 0 86 170 69 846.0 0 69 171 46 846.0 0 46 172 56 846.0 0 56 173 70 846.0 0 70 174 44 846.0 0 44 175 51 846.0 0 51 176 73 846.0 0 73 177 97 846.0 0 97 178 119 846.0 0 119 179 106 846.0 0 106 180 131 846.0 0 131 181 177 846.0 0 177 182 433 846.0 0 433 183 67 846.0 0 67 184 72 846.0 0 72 185 86 846.0 0 86 186 51 846.0 0 51 187 36 846.0 0 36 188 71 846.0 0 71 189 81 846.0 0 81 190 99 846.0 0 99 191 72 846.0 0 72 192 67 846.0 0 67 193 52 846.0 0 52 194 45 846.0 0 45 195 49 846.0 0 49 196 70 846.0 0 70 197 85 846.0 0 85 198 47 846.0 0 47 199 42 846.0 0 42 200 96 846.0 0 96 201 94 846.0 0 94 202 126 846.0 0 126 203 98 846.0 0 98 204 93 846.0 0 93 205 122 846.0 0 122 206 141 846.0 0 141 207 83 846.0 0 83 208 109 846.0 0 109 209 72 846.0 0 72 210 79 846.0 0 79 211 99 846.0 0 99 212 117 846.0 0 117 213 77 846.0 0 77 214 111 846.0 0 111 215 110 846.0 0 110 216 109 846.0 0 109 217 77 846.0 0 77 218 69 846.0 0 69 219 84 846.0 0 84 220 104 846.0 0 104 221 102 846.0 0 102 222 58 846.0 0 58 223 154 846.0 0 154 224 617 846.0 0 617 225 353 846.0 0 353 226 242 846.0 0 242 227 104 846.0 0 104 228 63 846.0 0 63 229 96 846.0 0 96 230 62 846.0 0 62 231 58 846.0 0 58 232 60 846.0 0 60 233 125 846.0 0 125 234 174 846.0 0 174 235 180 846.0 0 180 236 125 846.0 0 125 237 95 846.0 0 95 238 61 846.0 0 61 239 75 846.0 0 75 240 68 846.0 0 68 241 92 846.0 0 92 242 98 846.0 0 98 243 166 846.0 0 166 244 119 846.0 0 119 245 181 846.0 0 181 246 92 846.0 0 92 247 47 846.0 0 47 248 22 846.0 0 22 249 19 846.0 0 19 250 51 846.0 0 51 251 85 846.0 0 85 252 134 846.0 0 134 253 92 846.0 0 92 254 142 846.0 0 142 255 157 846.0 0 157 256 118 846.0 0 118 257 84 846.0 0 84 258 74 846.0 0 74 259 93 846.0 0 93 260 98 846.0 0 98 261 115 846.0 0 115 262 91 846.0 0 91 263 88 846.0 0 88 264 116 846.0 0 116 265 115 846.0 0 115 266 85 846.0 0 85 267 38 846.0 0 38 268 51 846.0 0 51 269 69 846.0 0 69 270 112 846.0 0 112 271 167 846.0 0 167 272 440 846.0 0 440 273 184 846.0 0 184 274 101 846.0 0 101 275 116 846.0 0 116 276 71 846.0 0 71 277 104 846.0 0 104 278 101 846.0 0 101 279 75 846.0 0 75 280 74 846.0 0 74 281 104 846.0 0 104 282 138 846.0 0 138 283 151 846.0 0 151 284 122 846.0 0 122 285 81 846.0 0 81 286 71 846.0 0 71 287 40 846.0 0 40 288 45 846.0 0 45 289 65 846.0 0 65 290 87 846.0 0 87 291 152 846.0 0 152 292 54 846.0 0 54 293 55 846.0 0 55 294 46 846.0 0 46 295 70 846.0 0 70 296 70 846.0 0 70 297 72 846.0 0 72 298 109 846.0 0 109 299 69 846.0 0 69 300 119 846.0 0 119 301 13 846.0 0 13 Finished in 15.22 s (39 us/read; 1.55 M reads/minute). === Summary === Total reads processed: 393,944 Reads with adapters: 30,489 (7.7%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 30,479 (7.7%) Total basepairs processed: 76,404,451 bp Total written (filtered): 8,290,476 bp (10.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 30489 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 3 0.0 3 0 0 3 19 80 0.0 3 0 77 1 0 2 20 30283 0.0 4 29909 350 19 2 3 21 77 0.0 4 15 61 1 22 9 0.0 4 0 0 7 2 23 13 0.0 4 1 0 0 10 2 24 5 0.0 4 0 1 0 0 4 27 1 0.0 4 1 31 1 0.0 4 1 37 1 0.0 4 0 0 0 0 1 39 1 0.0 4 1 57 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 83 2 0.0 4 0 0 0 0 2 84 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 0 1 183 2 0.0 4 0 0 0 0 2 184 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.03 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 30,479 Reads with adapters: 7,599 (24.9%) Reads written (passing filters): 30,479 (100.0%) Total basepairs processed: 8,290,476 bp Total written (filtered): 6,450,290 bp (77.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7599 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.6% C: 0.3% G: 90.9% T: 4.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 7 476.2 0 7 4 4 119.1 0 4 6 1 7.4 0 1 9 80 0.1 0 6 74 10 413 0.0 1 18 395 11 65 0.0 1 0 65 12 1 0.0 1 0 1 150 2 0.0 1 0 2 228 1 0.0 1 1 256 2 0.0 1 2 257 321 0.0 1 304 17 258 4 0.0 1 4 259 4 0.0 1 4 260 17 0.0 1 17 261 6215 0.0 1 5995 220 262 83 0.0 1 76 7 263 34 0.0 1 32 2 264 31 0.0 1 31 266 301 0.0 1 294 7 267 4 0.0 1 4 268 2 0.0 1 2 269 6 0.0 1 6 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.