This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 91.05 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 305,271 (8.8%) Reads that were too short: 140,703 (4.1%) Reads written (passing filters): 164,568 (4.7%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 26,859,485 bp (2.6%) === Adapter 1 === Sequence: ACCATA; Type: regular 5'; Length: 6; Trimmed: 305271 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 98 846.0 0 98 7 5859 846.0 0 5859 8 56 846.0 0 56 9 43 846.0 0 43 10 57 846.0 0 57 11 48 846.0 0 48 12 43 846.0 0 43 13 57 846.0 0 57 14 55 846.0 0 55 15 36 846.0 0 36 16 41 846.0 0 41 17 45 846.0 0 45 18 55 846.0 0 55 19 53 846.0 0 53 20 55 846.0 0 55 21 52 846.0 0 52 22 63 846.0 0 63 23 48 846.0 0 48 24 51 846.0 0 51 25 57 846.0 0 57 26 47 846.0 0 47 27 47 846.0 0 47 28 59 846.0 0 59 29 50 846.0 0 50 30 36 846.0 0 36 31 48 846.0 0 48 32 51 846.0 0 51 33 40 846.0 0 40 34 51 846.0 0 51 35 51 846.0 0 51 36 47 846.0 0 47 37 40 846.0 0 40 38 39 846.0 0 39 39 50 846.0 0 50 40 57 846.0 0 57 41 59 846.0 0 59 42 605 846.0 0 605 43 70 846.0 0 70 44 56 846.0 0 56 45 57 846.0 0 57 46 46 846.0 0 46 47 45 846.0 0 45 48 55 846.0 0 55 49 40 846.0 0 40 50 39 846.0 0 39 51 64 846.0 0 64 52 57 846.0 0 57 53 35 846.0 0 35 54 37 846.0 0 37 55 53 846.0 0 53 56 267 846.0 0 267 57 304 846.0 0 304 58 61 846.0 0 61 59 48 846.0 0 48 60 48 846.0 0 48 61 39 846.0 0 39 62 45 846.0 0 45 63 39 846.0 0 39 64 44 846.0 0 44 65 49 846.0 0 49 66 110 846.0 0 110 67 64 846.0 0 64 68 77 846.0 0 77 69 84 846.0 0 84 70 69 846.0 0 69 71 72 846.0 0 72 72 323 846.0 0 323 73 369 846.0 0 369 74 673 846.0 0 673 75 427 846.0 0 427 76 817 846.0 0 817 77 934 846.0 0 934 78 953 846.0 0 953 79 259 846.0 0 259 80 228 846.0 0 228 81 294 846.0 0 294 82 405 846.0 0 405 83 528 846.0 0 528 84 478 846.0 0 478 85 478 846.0 0 478 86 363 846.0 0 363 87 1498 846.0 0 1498 88 822 846.0 0 822 89 811 846.0 0 811 90 653 846.0 0 653 91 432 846.0 0 432 92 278 846.0 0 278 93 1473 846.0 0 1473 94 1434 846.0 0 1434 95 1100 846.0 0 1100 96 571 846.0 0 571 97 307 846.0 0 307 98 567 846.0 0 567 99 359 846.0 0 359 100 166 846.0 0 166 101 337 846.0 0 337 102 458 846.0 0 458 103 639 846.0 0 639 104 712 846.0 0 712 105 821 846.0 0 821 106 718 846.0 0 718 107 1592 846.0 0 1592 108 1841 846.0 0 1841 109 1580 846.0 0 1580 110 512 846.0 0 512 111 576 846.0 0 576 112 558 846.0 0 558 113 695 846.0 0 695 114 686 846.0 0 686 115 525 846.0 0 525 116 420 846.0 0 420 117 360 846.0 0 360 118 381 846.0 0 381 119 621 846.0 0 621 120 1170 846.0 0 1170 121 1061 846.0 0 1061 122 585 846.0 0 585 123 655 846.0 0 655 124 667 846.0 0 667 125 806 846.0 0 806 126 1110 846.0 0 1110 127 1265 846.0 0 1265 128 1254 846.0 0 1254 129 1317 846.0 0 1317 130 578 846.0 0 578 131 455 846.0 0 455 132 521 846.0 0 521 133 1993 846.0 0 1993 134 2842 846.0 0 2842 135 3387 846.0 0 3387 136 4596 846.0 0 4596 137 891 846.0 0 891 138 650 846.0 0 650 139 937 846.0 0 937 140 1265 846.0 0 1265 141 1362 846.0 0 1362 142 851 846.0 0 851 143 527 846.0 0 527 144 767 846.0 0 767 145 1199 846.0 0 1199 146 1363 846.0 0 1363 147 1396 846.0 0 1396 148 1243 846.0 0 1243 149 839 846.0 0 839 150 916 846.0 0 916 151 956 846.0 0 956 152 1516 846.0 0 1516 153 1704 846.0 0 1704 154 1597 846.0 0 1597 155 2019 846.0 0 2019 156 1982 846.0 0 1982 157 10043 846.0 0 10043 158 14641 846.0 0 14641 159 5026 846.0 0 5026 160 1383 846.0 0 1383 161 1310 846.0 0 1310 162 1030 846.0 0 1030 163 730 846.0 0 730 164 1058 846.0 0 1058 165 1586 846.0 0 1586 166 1389 846.0 0 1389 167 977 846.0 0 977 168 674 846.0 0 674 169 756 846.0 0 756 170 716 846.0 0 716 171 871 846.0 0 871 172 844 846.0 0 844 173 1997 846.0 0 1997 174 2293 846.0 0 2293 175 1424 846.0 0 1424 176 811 846.0 0 811 177 544 846.0 0 544 178 534 846.0 0 534 179 616 846.0 0 616 180 1106 846.0 0 1106 181 1628 846.0 0 1628 182 1267 846.0 0 1267 183 844 846.0 0 844 184 813 846.0 0 813 185 1061 846.0 0 1061 186 1845 846.0 0 1845 187 1067 846.0 0 1067 188 764 846.0 0 764 189 693 846.0 0 693 190 1002 846.0 0 1002 191 1510 846.0 0 1510 192 1171 846.0 0 1171 193 1378 846.0 0 1378 194 809 846.0 0 809 195 819 846.0 0 819 196 467 846.0 0 467 197 477 846.0 0 477 198 935 846.0 0 935 199 929 846.0 0 929 200 1126 846.0 0 1126 201 1007 846.0 0 1007 202 918 846.0 0 918 203 1397 846.0 0 1397 204 1030 846.0 0 1030 205 786 846.0 0 786 206 1010 846.0 0 1010 207 1072 846.0 0 1072 208 1052 846.0 0 1052 209 714 846.0 0 714 210 731 846.0 0 731 211 594 846.0 0 594 212 712 846.0 0 712 213 976 846.0 0 976 214 1106 846.0 0 1106 215 942 846.0 0 942 216 1062 846.0 0 1062 217 952 846.0 0 952 218 958 846.0 0 958 219 1303 846.0 0 1303 220 1427 846.0 0 1427 221 1275 846.0 0 1275 222 776 846.0 0 776 223 589 846.0 0 589 224 847 846.0 0 847 225 847 846.0 0 847 226 1015 846.0 0 1015 227 1073 846.0 0 1073 228 1093 846.0 0 1093 229 1017 846.0 0 1017 230 1213 846.0 0 1213 231 1060 846.0 0 1060 232 1131 846.0 0 1131 233 1202 846.0 0 1202 234 1088 846.0 0 1088 235 992 846.0 0 992 236 1136 846.0 0 1136 237 990 846.0 0 990 238 746 846.0 0 746 239 954 846.0 0 954 240 1085 846.0 0 1085 241 963 846.0 0 963 242 972 846.0 0 972 243 942 846.0 0 942 244 952 846.0 0 952 245 811 846.0 0 811 246 995 846.0 0 995 247 1024 846.0 0 1024 248 1083 846.0 0 1083 249 546 846.0 0 546 250 715 846.0 0 715 251 849 846.0 0 849 252 1924 846.0 0 1924 253 2579 846.0 0 2579 254 1162 846.0 0 1162 255 1076 846.0 0 1076 256 1275 846.0 0 1275 257 1410 846.0 0 1410 258 1361 846.0 0 1361 259 952 846.0 0 952 260 870 846.0 0 870 261 867 846.0 0 867 262 828 846.0 0 828 263 814 846.0 0 814 264 794 846.0 0 794 265 666 846.0 0 666 266 1466 846.0 0 1466 267 1711 846.0 0 1711 268 2416 846.0 0 2416 269 2438 846.0 0 2438 270 1272 846.0 0 1272 271 1178 846.0 0 1178 272 1315 846.0 0 1315 273 1249 846.0 0 1249 274 916 846.0 0 916 275 1488 846.0 0 1488 276 4038 846.0 0 4038 277 6929 846.0 0 6929 278 2544 846.0 0 2544 279 2220 846.0 0 2220 280 2599 846.0 0 2599 281 2091 846.0 0 2091 282 1073 846.0 0 1073 283 890 846.0 0 890 284 1396 846.0 0 1396 285 1465 846.0 0 1465 286 1077 846.0 0 1077 287 1563 846.0 0 1563 288 1334 846.0 0 1334 289 1389 846.0 0 1389 290 1071 846.0 0 1071 291 995 846.0 0 995 292 1222 846.0 0 1222 293 1779 846.0 0 1779 294 2281 846.0 0 2281 295 3493 846.0 0 3493 296 5130 846.0 0 5130 297 8848 846.0 0 8848 298 746 846.0 0 746 299 803 846.0 0 803 300 1110 846.0 0 1110 301 1867 846.0 0 1867 Finished in 5.09 s (31 us/read; 1.94 M reads/minute). === Summary === Total reads processed: 164,568 Reads with adapters: 2,363 (1.4%) Reads that were too short: 73 (0.0%) Reads written (passing filters): 2,290 (1.4%) Total basepairs processed: 26,859,485 bp Total written (filtered): 625,198 bp (2.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2363 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 12 0.0 3 0 9 2 1 17 2209 0.0 3 1847 329 29 4 18 6 0.0 3 3 1 0 2 19 1 0.0 3 0 0 1 20 5 0.0 3 2 0 0 3 21 2 0.0 3 0 0 0 2 22 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 26 2 0.0 3 0 0 0 2 27 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 30 2 0.0 3 0 0 0 2 32 12 0.0 3 0 0 0 12 33 1 0.0 3 0 0 0 1 35 4 0.0 3 0 0 0 4 36 1 0.0 3 0 0 0 1 37 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 45 2 0.0 3 0 0 0 2 46 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 4 0.0 3 0 0 0 4 58 8 0.0 3 0 0 0 8 59 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 83 3 0.0 3 0 0 0 3 84 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 10 0.0 3 0 0 0 10 93 4 0.0 3 0 0 0 4 94 1 0.0 3 0 0 0 1 97 2 0.0 3 0 0 0 2 105 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 5 0.0 3 0 0 0 5 132 2 0.0 3 0 0 0 2 133 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 138 3 0.0 3 0 0 0 3 141 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 146 3 0.0 3 0 0 0 3 148 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 1 157 1 0.0 3 0 0 0 1 159 3 0.0 3 0 0 0 3 161 3 0.0 3 0 0 0 3 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 169 2 0.0 3 0 0 0 2 176 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 2,290 Reads with adapters: 189 (8.3%) Reads written (passing filters): 2,290 (100.0%) Total basepairs processed: 625,198 bp Total written (filtered): 624,631 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 189 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 28.0% C: 63.0% G: 5.8% T: 3.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 189 35.8 0 189