This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GACGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 89.36 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 407,900 (11.8%) Reads that were too short: 35,954 (1.0%) Reads written (passing filters): 371,946 (10.7%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 83,153,470 bp (8.0%) === Adapter 1 === Sequence: GACGGC; Type: regular 5'; Length: 6; Trimmed: 407900 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 506 846.0 0 506 7 3026 846.0 0 3026 8 632 846.0 0 632 9 79406 846.0 0 79406 10 150 846.0 0 150 11 46 846.0 0 46 12 42 846.0 0 42 13 39 846.0 0 39 14 39 846.0 0 39 15 42 846.0 0 42 16 53 846.0 0 53 17 59 846.0 0 59 18 61 846.0 0 61 19 59 846.0 0 59 20 66 846.0 0 66 21 61 846.0 0 61 22 53 846.0 0 53 23 51 846.0 0 51 24 48 846.0 0 48 25 42 846.0 0 42 26 50 846.0 0 50 27 52 846.0 0 52 28 59 846.0 0 59 29 56 846.0 0 56 30 56 846.0 0 56 31 48 846.0 0 48 32 49 846.0 0 49 33 67 846.0 0 67 34 41 846.0 0 41 35 92 846.0 0 92 36 61 846.0 0 61 37 52 846.0 0 52 38 68 846.0 0 68 39 34 846.0 0 34 40 68 846.0 0 68 41 87 846.0 0 87 42 80 846.0 0 80 43 77 846.0 0 77 44 56 846.0 0 56 45 65 846.0 0 65 46 69 846.0 0 69 47 59 846.0 0 59 48 57 846.0 0 57 49 42 846.0 0 42 50 77 846.0 0 77 51 89 846.0 0 89 52 76 846.0 0 76 53 94 846.0 0 94 54 78 846.0 0 78 55 145 846.0 0 145 56 74 846.0 0 74 57 60 846.0 0 60 58 72 846.0 0 72 59 148 846.0 0 148 60 122 846.0 0 122 61 350 846.0 0 350 62 238 846.0 0 238 63 100 846.0 0 100 64 67 846.0 0 67 65 205 846.0 0 205 66 89 846.0 0 89 67 157 846.0 0 157 68 226 846.0 0 226 69 466 846.0 0 466 70 743 846.0 0 743 71 1824 846.0 0 1824 72 2156 846.0 0 2156 73 605 846.0 0 605 74 529 846.0 0 529 75 1104 846.0 0 1104 76 1362 846.0 0 1362 77 789 846.0 0 789 78 931 846.0 0 931 79 834 846.0 0 834 80 864 846.0 0 864 81 1076 846.0 0 1076 82 1523 846.0 0 1523 83 1711 846.0 0 1711 84 3355 846.0 0 3355 85 111892 846.0 0 111892 86 31265 846.0 0 31265 87 76251 846.0 0 76251 88 328 846.0 0 328 89 47 846.0 0 47 90 71 846.0 0 71 91 63 846.0 0 63 92 54 846.0 0 54 93 55 846.0 0 55 94 62 846.0 0 62 95 48 846.0 0 48 96 56 846.0 0 56 97 52 846.0 0 52 98 68 846.0 0 68 99 54 846.0 0 54 100 65 846.0 0 65 101 56 846.0 0 56 102 52 846.0 0 52 103 43 846.0 0 43 104 29 846.0 0 29 105 31 846.0 0 31 106 36 846.0 0 36 107 44 846.0 0 44 108 47 846.0 0 47 109 40 846.0 0 40 110 27 846.0 0 27 111 49 846.0 0 49 112 37 846.0 0 37 113 52 846.0 0 52 114 54 846.0 0 54 115 65 846.0 0 65 116 45 846.0 0 45 117 46 846.0 0 46 118 67 846.0 0 67 119 43 846.0 0 43 120 48 846.0 0 48 121 38 846.0 0 38 122 56 846.0 0 56 123 49 846.0 0 49 124 65 846.0 0 65 125 43 846.0 0 43 126 52 846.0 0 52 127 65 846.0 0 65 128 213 846.0 0 213 129 62 846.0 0 62 130 68 846.0 0 68 131 57 846.0 0 57 132 94 846.0 0 94 133 78 846.0 0 78 134 50 846.0 0 50 135 70 846.0 0 70 136 89 846.0 0 89 137 76 846.0 0 76 138 76 846.0 0 76 139 66 846.0 0 66 140 60 846.0 0 60 141 60 846.0 0 60 142 74 846.0 0 74 143 91 846.0 0 91 144 70 846.0 0 70 145 63 846.0 0 63 146 57 846.0 0 57 147 75 846.0 0 75 148 78 846.0 0 78 149 346 846.0 0 346 150 779 846.0 0 779 151 821 846.0 0 821 152 846 846.0 0 846 153 460 846.0 0 460 154 262 846.0 0 262 155 388 846.0 0 388 156 114 846.0 0 114 157 110 846.0 0 110 158 76 846.0 0 76 159 74 846.0 0 74 160 116 846.0 0 116 161 181 846.0 0 181 162 368 846.0 0 368 163 109 846.0 0 109 164 139 846.0 0 139 165 232 846.0 0 232 166 132 846.0 0 132 167 126 846.0 0 126 168 266 846.0 0 266 169 210 846.0 0 210 170 214 846.0 0 214 171 267 846.0 0 267 172 354 846.0 0 354 173 1159 846.0 0 1159 174 1177 846.0 0 1177 175 4898 846.0 0 4898 176 9166 846.0 0 9166 177 15011 846.0 0 15011 178 94 846.0 0 94 179 48 846.0 0 48 180 69 846.0 0 69 181 108 846.0 0 108 182 604 846.0 0 604 183 62 846.0 0 62 184 54 846.0 0 54 185 56 846.0 0 56 186 73 846.0 0 73 187 44 846.0 0 44 188 38 846.0 0 38 189 65 846.0 0 65 190 51 846.0 0 51 191 42 846.0 0 42 192 39 846.0 0 39 193 59 846.0 0 59 194 48 846.0 0 48 195 48 846.0 0 48 196 78 846.0 0 78 197 59 846.0 0 59 198 51 846.0 0 51 199 38 846.0 0 38 200 61 846.0 0 61 201 54 846.0 0 54 202 60 846.0 0 60 203 72 846.0 0 72 204 63 846.0 0 63 205 59 846.0 0 59 206 77 846.0 0 77 207 80 846.0 0 80 208 72 846.0 0 72 209 135 846.0 0 135 210 185 846.0 0 185 211 321 846.0 0 321 212 476 846.0 0 476 213 20073 846.0 0 20073 214 1021 846.0 0 1021 215 7622 846.0 0 7622 216 96 846.0 0 96 217 30 846.0 0 30 218 79 846.0 0 79 219 37 846.0 0 37 220 40 846.0 0 40 221 55 846.0 0 55 222 53 846.0 0 53 223 24 846.0 0 24 224 34 846.0 0 34 225 52 846.0 0 52 226 42 846.0 0 42 227 43 846.0 0 43 228 93 846.0 0 93 229 53 846.0 0 53 230 62 846.0 0 62 231 127 846.0 0 127 232 144 846.0 0 144 233 86 846.0 0 86 234 68 846.0 0 68 235 75 846.0 0 75 236 40 846.0 0 40 237 59 846.0 0 59 238 44 846.0 0 44 239 40 846.0 0 40 240 36 846.0 0 36 241 43 846.0 0 43 242 51 846.0 0 51 243 51 846.0 0 51 244 72 846.0 0 72 245 48 846.0 0 48 246 51 846.0 0 51 247 41 846.0 0 41 248 43 846.0 0 43 249 31 846.0 0 31 250 46 846.0 0 46 251 116 846.0 0 116 252 138 846.0 0 138 253 132 846.0 0 132 254 107 846.0 0 107 255 122 846.0 0 122 256 323 846.0 0 323 257 120 846.0 0 120 258 96 846.0 0 96 259 58 846.0 0 58 260 44 846.0 0 44 261 61 846.0 0 61 262 37 846.0 0 37 263 33 846.0 0 33 264 31 846.0 0 31 265 47 846.0 0 47 266 32 846.0 0 32 267 36 846.0 0 36 268 36 846.0 0 36 269 26 846.0 0 26 270 37 846.0 0 37 271 57 846.0 0 57 272 37 846.0 0 37 273 26 846.0 0 26 274 36 846.0 0 36 275 26 846.0 0 26 276 76 846.0 0 76 277 34 846.0 0 34 278 82 846.0 0 82 279 58 846.0 0 58 280 28 846.0 0 28 281 43 846.0 0 43 282 37 846.0 0 37 283 65 846.0 0 65 284 31 846.0 0 31 285 67 846.0 0 67 286 81 846.0 0 81 287 61 846.0 0 61 288 82 846.0 0 82 289 31 846.0 0 31 290 67 846.0 0 67 291 82 846.0 0 82 292 51 846.0 0 51 293 45 846.0 0 45 294 50 846.0 0 50 295 25 846.0 0 25 296 64 846.0 0 64 297 140 846.0 0 140 298 306 846.0 0 306 299 142 846.0 0 142 300 55 846.0 0 55 301 32 846.0 0 32 Finished in 15.90 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 371,946 Reads with adapters: 2,675 (0.7%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 2,659 (0.7%) Total basepairs processed: 83,153,470 bp Total written (filtered): 706,483 bp (0.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2675 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 1 0.0 3 0 0 0 0 1 18 1 0.0 3 0 0 1 19 23 0.0 3 0 21 1 1 20 2410 0.0 4 2377 31 1 1 21 9 0.0 4 0 9 22 11 0.0 4 9 0 1 1 42 1 0.0 4 0 0 0 0 1 49 3 0.0 4 0 0 0 0 3 66 1 0.0 4 0 0 0 1 73 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 196 0.0 4 0 0 0 0 196 134 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 1 179 1 0.0 4 0 0 0 0 1 181 2 0.0 4 0 0 0 0 2 186 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.09 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 2,659 Reads with adapters: 40 (1.5%) Reads written (passing filters): 2,659 (100.0%) Total basepairs processed: 706,483 bp Total written (filtered): 697,182 bp (98.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 40 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 67.5% C: 10.0% G: 20.0% T: 2.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 10 1 0.0 1 0 1 11 2 0.0 1 0 2 12 2 0.0 1 0 2 260 2 0.0 1 1 1 261 19 0.0 1 17 2 262 2 0.0 1 2 263 1 0.0 1 1 265 1 0.0 1 1 268 1 0.0 1 1 271 2 0.0 1 2 272 7 0.0 1 7