This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 88.91 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 83,893 (2.4%) Reads that were too short: 24,092 (0.7%) Reads written (passing filters): 59,801 (1.7%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 12,943,839 bp (1.2%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 83893 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 840 846.0 0 840 7 310 846.0 0 310 8 15427 846.0 0 15427 9 13297 846.0 0 13297 10 54 846.0 0 54 11 9 846.0 0 9 12 9 846.0 0 9 13 11 846.0 0 11 14 14 846.0 0 14 15 19 846.0 0 19 16 4 846.0 0 4 17 4 846.0 0 4 18 12 846.0 0 12 19 93 846.0 0 93 20 170 846.0 0 170 21 5 846.0 0 5 22 9 846.0 0 9 23 9 846.0 0 9 24 3 846.0 0 3 25 7 846.0 0 7 26 20 846.0 0 20 27 4 846.0 0 4 28 4 846.0 0 4 29 8 846.0 0 8 30 7 846.0 0 7 31 5 846.0 0 5 32 2 846.0 0 2 33 2 846.0 0 2 34 7 846.0 0 7 35 5 846.0 0 5 36 1 846.0 0 1 37 7 846.0 0 7 38 1 846.0 0 1 39 6 846.0 0 6 40 5 846.0 0 5 41 5 846.0 0 5 42 2 846.0 0 2 43 72 846.0 0 72 44 46 846.0 0 46 45 97 846.0 0 97 46 14 846.0 0 14 47 7 846.0 0 7 48 9 846.0 0 9 49 7 846.0 0 7 50 5 846.0 0 5 51 5 846.0 0 5 52 8 846.0 0 8 53 5 846.0 0 5 54 4 846.0 0 4 55 12 846.0 0 12 56 10 846.0 0 10 57 7 846.0 0 7 58 7 846.0 0 7 59 10 846.0 0 10 60 24 846.0 0 24 61 8 846.0 0 8 62 9 846.0 0 9 63 11 846.0 0 11 64 2 846.0 0 2 65 11 846.0 0 11 66 5 846.0 0 5 67 9 846.0 0 9 68 12 846.0 0 12 69 7 846.0 0 7 70 8 846.0 0 8 71 10 846.0 0 10 72 24 846.0 0 24 73 31 846.0 0 31 74 63 846.0 0 63 75 76 846.0 0 76 76 181 846.0 0 181 77 2194 846.0 0 2194 78 593 846.0 0 593 79 629 846.0 0 629 80 17 846.0 0 17 81 14 846.0 0 14 82 32 846.0 0 32 83 39 846.0 0 39 84 109 846.0 0 109 85 62 846.0 0 62 86 31 846.0 0 31 87 12 846.0 0 12 88 18 846.0 0 18 89 28 846.0 0 28 90 32 846.0 0 32 91 82 846.0 0 82 92 89 846.0 0 89 93 78 846.0 0 78 94 69 846.0 0 69 95 123 846.0 0 123 96 49 846.0 0 49 97 35 846.0 0 35 98 40 846.0 0 40 99 23 846.0 0 23 100 10 846.0 0 10 101 12 846.0 0 12 102 22 846.0 0 22 103 6 846.0 0 6 104 2 846.0 0 2 105 5 846.0 0 5 106 19 846.0 0 19 107 18 846.0 0 18 108 19 846.0 0 19 109 14 846.0 0 14 110 14 846.0 0 14 111 14 846.0 0 14 112 31 846.0 0 31 113 38 846.0 0 38 114 79 846.0 0 79 115 95 846.0 0 95 116 63 846.0 0 63 117 40 846.0 0 40 118 30 846.0 0 30 119 26 846.0 0 26 120 23 846.0 0 23 121 29 846.0 0 29 122 43 846.0 0 43 123 54 846.0 0 54 124 44 846.0 0 44 125 42 846.0 0 42 126 63 846.0 0 63 127 42 846.0 0 42 128 68 846.0 0 68 129 108 846.0 0 108 130 32 846.0 0 32 131 35 846.0 0 35 132 21 846.0 0 21 133 22 846.0 0 22 134 61 846.0 0 61 135 178 846.0 0 178 136 244 846.0 0 244 137 119 846.0 0 119 138 55 846.0 0 55 139 166 846.0 0 166 140 97 846.0 0 97 141 83 846.0 0 83 142 292 846.0 0 292 143 67 846.0 0 67 144 27 846.0 0 27 145 28 846.0 0 28 146 45 846.0 0 45 147 29 846.0 0 29 148 80 846.0 0 80 149 46 846.0 0 46 150 59 846.0 0 59 151 29 846.0 0 29 152 28 846.0 0 28 153 44 846.0 0 44 154 47 846.0 0 47 155 57 846.0 0 57 156 46 846.0 0 46 157 38 846.0 0 38 158 167 846.0 0 167 159 31 846.0 0 31 160 25 846.0 0 25 161 32 846.0 0 32 162 58 846.0 0 58 163 62 846.0 0 62 164 90 846.0 0 90 165 59 846.0 0 59 166 67 846.0 0 67 167 58 846.0 0 58 168 51 846.0 0 51 169 39 846.0 0 39 170 42 846.0 0 42 171 55 846.0 0 55 172 117 846.0 0 117 173 270 846.0 0 270 174 289 846.0 0 289 175 692 846.0 0 692 176 82 846.0 0 82 177 67 846.0 0 67 178 89 846.0 0 89 179 80 846.0 0 80 180 321 846.0 0 321 181 932 846.0 0 932 182 262 846.0 0 262 183 59 846.0 0 59 184 80 846.0 0 80 185 58 846.0 0 58 186 62 846.0 0 62 187 91 846.0 0 91 188 157 846.0 0 157 189 213 846.0 0 213 190 351 846.0 0 351 191 2825 846.0 0 2825 192 9908 846.0 0 9908 193 2637 846.0 0 2637 194 113 846.0 0 113 195 51 846.0 0 51 196 59 846.0 0 59 197 80 846.0 0 80 198 47 846.0 0 47 199 47 846.0 0 47 200 75 846.0 0 75 201 92 846.0 0 92 202 332 846.0 0 332 203 267 846.0 0 267 204 203 846.0 0 203 205 151 846.0 0 151 206 29 846.0 0 29 207 60 846.0 0 60 208 212 846.0 0 212 209 139 846.0 0 139 210 101 846.0 0 101 211 58 846.0 0 58 212 29 846.0 0 29 213 43 846.0 0 43 214 29 846.0 0 29 215 47 846.0 0 47 216 52 846.0 0 52 217 43 846.0 0 43 218 32 846.0 0 32 219 20 846.0 0 20 220 30 846.0 0 30 221 29 846.0 0 29 222 23 846.0 0 23 223 25 846.0 0 25 224 15 846.0 0 15 225 39 846.0 0 39 226 43 846.0 0 43 227 81 846.0 0 81 228 27 846.0 0 27 229 43 846.0 0 43 230 39 846.0 0 39 231 30 846.0 0 30 232 50 846.0 0 50 233 30 846.0 0 30 234 52 846.0 0 52 235 40 846.0 0 40 236 53 846.0 0 53 237 62 846.0 0 62 238 23 846.0 0 23 239 20 846.0 0 20 240 27 846.0 0 27 241 27 846.0 0 27 242 21 846.0 0 21 243 21 846.0 0 21 244 31 846.0 0 31 245 29 846.0 0 29 246 24 846.0 0 24 247 43 846.0 0 43 248 80 846.0 0 80 249 101 846.0 0 101 250 177 846.0 0 177 251 126 846.0 0 126 252 142 846.0 0 142 253 103 846.0 0 103 254 118 846.0 0 118 255 68 846.0 0 68 256 46 846.0 0 46 257 52 846.0 0 52 258 501 846.0 0 501 259 1357 846.0 0 1357 260 969 846.0 0 969 261 1732 846.0 0 1732 262 2042 846.0 0 2042 263 567 846.0 0 567 264 1133 846.0 0 1133 265 263 846.0 0 263 266 695 846.0 0 695 267 30 846.0 0 30 268 68 846.0 0 68 269 147 846.0 0 147 270 1009 846.0 0 1009 271 114 846.0 0 114 272 110 846.0 0 110 273 48 846.0 0 48 274 66 846.0 0 66 275 212 846.0 0 212 276 260 846.0 0 260 277 222 846.0 0 222 278 41 846.0 0 41 279 55 846.0 0 55 280 78 846.0 0 78 281 169 846.0 0 169 282 142 846.0 0 142 283 463 846.0 0 463 284 1191 846.0 0 1191 285 752 846.0 0 752 286 1782 846.0 0 1782 287 149 846.0 0 149 288 156 846.0 0 156 289 772 846.0 0 772 290 1074 846.0 0 1074 291 167 846.0 0 167 292 48 846.0 0 48 293 75 846.0 0 75 294 82 846.0 0 82 295 505 846.0 0 505 296 365 846.0 0 365 297 441 846.0 0 441 298 47 846.0 0 47 299 23 846.0 0 23 300 19 846.0 0 19 301 114 846.0 0 114 Finished in 2.43 s (41 us/read; 1.48 M reads/minute). === Summary === Total reads processed: 59,801 Reads with adapters: 16,154 (27.0%) Reads that were too short: 40 (0.1%) Reads written (passing filters): 16,114 (26.9%) Total basepairs processed: 12,943,839 bp Total written (filtered): 4,440,093 bp (34.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 16154 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 6 0.0 3 0 0 4 2 16 125 0.0 3 0 112 6 7 17 15203 0.0 3 14700 463 34 6 18 75 0.0 3 7 25 19 24 19 43 0.0 3 1 7 12 23 20 633 0.0 3 605 9 0 19 21 10 0.0 3 0 2 0 8 22 1 0.0 3 0 0 1 30 6 0.0 3 0 0 0 6 44 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 49 3 0.0 3 0 0 0 3 50 1 0.0 3 0 0 0 1 52 4 0.0 3 0 0 0 4 64 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 119 2 0.0 3 0 0 0 2 124 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 221 3 0.0 3 0 0 0 3 271 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 288 2 0.0 3 0 0 0 2 289 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.56 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 16,114 Reads with adapters: 288 (1.8%) Reads written (passing filters): 16,114 (100.0%) Total basepairs processed: 4,440,093 bp Total written (filtered): 4,439,223 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 288 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 69.4% C: 11.5% G: 7.3% T: 11.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 283 251.8 0 283 4 4 62.9 0 4 5 1 15.7 0 1