This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 88.99 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 38,059 (1.1%) Reads that were too short: 5,603 (0.2%) Reads written (passing filters): 32,456 (0.9%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 8,746,162 bp (0.8%) === Adapter 1 === Sequence: GTGATC; Type: regular 5'; Length: 6; Trimmed: 38059 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 126 846.0 0 126 7 716 846.0 0 716 8 8324 846.0 0 8324 9 15928 846.0 0 15928 10 71 846.0 0 71 11 8 846.0 0 8 12 6 846.0 0 6 13 10 846.0 0 10 14 7 846.0 0 7 15 8 846.0 0 8 16 7 846.0 0 7 17 13 846.0 0 13 18 9 846.0 0 9 19 4 846.0 0 4 20 9 846.0 0 9 21 8 846.0 0 8 22 12 846.0 0 12 23 14 846.0 0 14 24 9 846.0 0 9 25 10 846.0 0 10 26 6 846.0 0 6 27 9 846.0 0 9 28 7 846.0 0 7 29 7 846.0 0 7 30 5 846.0 0 5 31 11 846.0 0 11 32 9 846.0 0 9 33 11 846.0 0 11 34 3 846.0 0 3 35 7 846.0 0 7 36 8 846.0 0 8 37 10 846.0 0 10 38 22 846.0 0 22 39 14 846.0 0 14 40 7 846.0 0 7 41 6 846.0 0 6 42 6 846.0 0 6 43 7 846.0 0 7 44 10 846.0 0 10 45 13 846.0 0 13 46 5 846.0 0 5 47 6 846.0 0 6 48 8 846.0 0 8 49 6 846.0 0 6 50 10 846.0 0 10 51 10 846.0 0 10 52 7 846.0 0 7 53 5 846.0 0 5 54 7 846.0 0 7 55 8 846.0 0 8 56 12 846.0 0 12 57 16 846.0 0 16 58 194 846.0 0 194 59 86 846.0 0 86 60 139 846.0 0 139 61 17 846.0 0 17 62 14 846.0 0 14 63 27 846.0 0 27 64 11 846.0 0 11 65 14 846.0 0 14 66 11 846.0 0 11 67 27 846.0 0 27 68 8 846.0 0 8 69 11 846.0 0 11 70 11 846.0 0 11 71 10 846.0 0 10 72 4 846.0 0 4 73 20 846.0 0 20 74 20 846.0 0 20 75 40 846.0 0 40 76 1414 846.0 0 1414 77 36 846.0 0 36 78 186 846.0 0 186 79 8 846.0 0 8 80 174 846.0 0 174 81 158 846.0 0 158 82 42 846.0 0 42 83 24 846.0 0 24 84 3 846.0 0 3 85 5 846.0 0 5 86 2 846.0 0 2 87 13 846.0 0 13 88 13 846.0 0 13 89 14 846.0 0 14 90 3 846.0 0 3 91 4 846.0 0 4 92 1 846.0 0 1 93 5 846.0 0 5 94 8 846.0 0 8 95 13 846.0 0 13 96 13 846.0 0 13 97 36 846.0 0 36 98 70 846.0 0 70 99 16 846.0 0 16 100 232 846.0 0 232 101 524 846.0 0 524 102 222 846.0 0 222 103 13 846.0 0 13 104 7 846.0 0 7 105 4 846.0 0 4 106 4 846.0 0 4 107 3 846.0 0 3 108 4 846.0 0 4 109 74 846.0 0 74 110 21 846.0 0 21 111 44 846.0 0 44 112 195 846.0 0 195 113 5 846.0 0 5 114 7 846.0 0 7 115 7 846.0 0 7 116 8 846.0 0 8 117 12 846.0 0 12 118 14 846.0 0 14 119 17 846.0 0 17 120 5 846.0 0 5 121 8 846.0 0 8 122 7 846.0 0 7 123 6 846.0 0 6 124 8 846.0 0 8 125 11 846.0 0 11 126 6 846.0 0 6 127 36 846.0 0 36 128 13 846.0 0 13 129 12 846.0 0 12 130 14 846.0 0 14 131 20 846.0 0 20 132 27 846.0 0 27 133 32 846.0 0 32 134 20 846.0 0 20 135 14 846.0 0 14 136 17 846.0 0 17 137 20 846.0 0 20 138 55 846.0 0 55 139 18 846.0 0 18 140 22 846.0 0 22 141 16 846.0 0 16 142 24 846.0 0 24 143 20 846.0 0 20 144 19 846.0 0 19 145 22 846.0 0 22 146 17 846.0 0 17 147 97 846.0 0 97 148 19 846.0 0 19 149 26 846.0 0 26 150 22 846.0 0 22 151 13 846.0 0 13 152 15 846.0 0 15 153 19 846.0 0 19 154 15 846.0 0 15 155 18 846.0 0 18 156 23 846.0 0 23 157 41 846.0 0 41 158 37 846.0 0 37 159 34 846.0 0 34 160 31 846.0 0 31 161 29 846.0 0 29 162 66 846.0 0 66 163 25 846.0 0 25 164 21 846.0 0 21 165 10 846.0 0 10 166 26 846.0 0 26 167 24 846.0 0 24 168 127 846.0 0 127 169 127 846.0 0 127 170 142 846.0 0 142 171 21 846.0 0 21 172 18 846.0 0 18 173 10 846.0 0 10 174 13 846.0 0 13 175 20 846.0 0 20 176 30 846.0 0 30 177 43 846.0 0 43 178 22 846.0 0 22 179 23 846.0 0 23 180 29 846.0 0 29 181 17 846.0 0 17 182 24 846.0 0 24 183 29 846.0 0 29 184 31 846.0 0 31 185 42 846.0 0 42 186 22 846.0 0 22 187 60 846.0 0 60 188 28 846.0 0 28 189 35 846.0 0 35 190 20 846.0 0 20 191 19 846.0 0 19 192 35 846.0 0 35 193 24 846.0 0 24 194 51 846.0 0 51 195 85 846.0 0 85 196 43 846.0 0 43 197 44 846.0 0 44 198 59 846.0 0 59 199 46 846.0 0 46 200 68 846.0 0 68 201 30 846.0 0 30 202 36 846.0 0 36 203 33 846.0 0 33 204 122 846.0 0 122 205 80 846.0 0 80 206 88 846.0 0 88 207 60 846.0 0 60 208 58 846.0 0 58 209 29 846.0 0 29 210 32 846.0 0 32 211 32 846.0 0 32 212 48 846.0 0 48 213 45 846.0 0 45 214 54 846.0 0 54 215 42 846.0 0 42 216 49 846.0 0 49 217 58 846.0 0 58 218 49 846.0 0 49 219 42 846.0 0 42 220 49 846.0 0 49 221 76 846.0 0 76 222 73 846.0 0 73 223 100 846.0 0 100 224 129 846.0 0 129 225 63 846.0 0 63 226 32 846.0 0 32 227 52 846.0 0 52 228 202 846.0 0 202 229 61 846.0 0 61 230 40 846.0 0 40 231 30 846.0 0 30 232 26 846.0 0 26 233 32 846.0 0 32 234 35 846.0 0 35 235 33 846.0 0 33 236 46 846.0 0 46 237 163 846.0 0 163 238 90 846.0 0 90 239 73 846.0 0 73 240 125 846.0 0 125 241 108 846.0 0 108 242 99 846.0 0 99 243 45 846.0 0 45 244 66 846.0 0 66 245 51 846.0 0 51 246 29 846.0 0 29 247 28 846.0 0 28 248 40 846.0 0 40 249 71 846.0 0 71 250 23 846.0 0 23 251 15 846.0 0 15 252 14 846.0 0 14 253 47 846.0 0 47 254 63 846.0 0 63 255 70 846.0 0 70 256 90 846.0 0 90 257 75 846.0 0 75 258 70 846.0 0 70 259 52 846.0 0 52 260 48 846.0 0 48 261 34 846.0 0 34 262 84 846.0 0 84 263 49 846.0 0 49 264 72 846.0 0 72 265 55 846.0 0 55 266 59 846.0 0 59 267 30 846.0 0 30 268 54 846.0 0 54 269 51 846.0 0 51 270 37 846.0 0 37 271 47 846.0 0 47 272 59 846.0 0 59 273 41 846.0 0 41 274 44 846.0 0 44 275 58 846.0 0 58 276 55 846.0 0 55 277 57 846.0 0 57 278 58 846.0 0 58 279 37 846.0 0 37 280 53 846.0 0 53 281 94 846.0 0 94 282 49 846.0 0 49 283 37 846.0 0 37 284 54 846.0 0 54 285 63 846.0 0 63 286 40 846.0 0 40 287 43 846.0 0 43 288 62 846.0 0 62 289 73 846.0 0 73 290 24 846.0 0 24 291 25 846.0 0 25 292 38 846.0 0 38 293 77 846.0 0 77 294 88 846.0 0 88 295 33 846.0 0 33 296 18 846.0 0 18 297 17 846.0 0 17 298 34 846.0 0 34 299 27 846.0 0 27 300 45 846.0 0 45 301 37 846.0 0 37 Finished in 1.61 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 32,456 Reads with adapters: 16,857 (51.9%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 16,852 (51.9%) Total basepairs processed: 8,746,162 bp Total written (filtered): 4,577,092 bp (52.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 16857 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 47 0.0 3 0 43 1 3 20 16123 0.0 4 15923 193 3 2 2 21 588 0.0 4 539 41 1 6 1 22 7 0.0 4 0 1 6 23 12 0.0 4 4 0 0 8 24 2 0.0 4 0 0 0 1 1 89 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 127 70 0.0 4 0 0 0 0 70 178 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.58 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 16,852 Reads with adapters: 487 (2.9%) Reads written (passing filters): 16,852 (100.0%) Total basepairs processed: 4,577,092 bp Total written (filtered): 4,545,791 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 487 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 21.6% C: 2.3% G: 75.2% T: 1.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 4 2 65.8 0 2 9 58 0.1 0 2 56 10 266 0.0 1 9 257 11 52 0.0 1 0 52 12 1 0.0 1 0 1 13 1 0.0 1 0 1 210 1 0.0 1 0 1 255 1 0.0 1 1 257 96 0.0 1 89 7 258 1 0.0 1 1 261 3 0.0 1 3 262 1 0.0 1 1 263 1 0.0 1 1 270 2 0.0 1 2 271 1 0.0 1 1