This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATTCTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 91.03 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 349,085 (10.1%) Reads that were too short: 26,194 (0.8%) Reads written (passing filters): 322,891 (9.3%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 53,254,105 bp (5.1%) === Adapter 1 === Sequence: ATTCTA; Type: regular 5'; Length: 6; Trimmed: 349085 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 101 846.0 0 101 7 8119 846.0 0 8119 8 8471 846.0 0 8471 9 17 846.0 0 17 10 9 846.0 0 9 11 10 846.0 0 10 12 35 846.0 0 35 13 60 846.0 0 60 14 15 846.0 0 15 15 10 846.0 0 10 16 7 846.0 0 7 17 8 846.0 0 8 18 5 846.0 0 5 19 6 846.0 0 6 20 18 846.0 0 18 21 7 846.0 0 7 22 7 846.0 0 7 23 7 846.0 0 7 24 8 846.0 0 8 25 10 846.0 0 10 26 13 846.0 0 13 27 18 846.0 0 18 28 12 846.0 0 12 29 8 846.0 0 8 30 3 846.0 0 3 31 14 846.0 0 14 32 5 846.0 0 5 33 5 846.0 0 5 34 24 846.0 0 24 35 38 846.0 0 38 36 2 846.0 0 2 37 4 846.0 0 4 38 9 846.0 0 9 39 3 846.0 0 3 40 7 846.0 0 7 41 5 846.0 0 5 42 9 846.0 0 9 43 23 846.0 0 23 44 16 846.0 0 16 45 4 846.0 0 4 46 6 846.0 0 6 47 6 846.0 0 6 48 9 846.0 0 9 49 3 846.0 0 3 50 5 846.0 0 5 51 7 846.0 0 7 52 3 846.0 0 3 53 9 846.0 0 9 54 11 846.0 0 11 55 10 846.0 0 10 56 10 846.0 0 10 57 3 846.0 0 3 58 12 846.0 0 12 59 5 846.0 0 5 60 12 846.0 0 12 61 11 846.0 0 11 62 8 846.0 0 8 63 12 846.0 0 12 64 11 846.0 0 11 65 8 846.0 0 8 66 11 846.0 0 11 67 17 846.0 0 17 68 13 846.0 0 13 69 6 846.0 0 6 70 13 846.0 0 13 71 22 846.0 0 22 72 16 846.0 0 16 73 16 846.0 0 16 74 9 846.0 0 9 75 18 846.0 0 18 76 2 846.0 0 2 77 2 846.0 0 2 78 11 846.0 0 11 79 5 846.0 0 5 80 37 846.0 0 37 81 42 846.0 0 42 82 62 846.0 0 62 83 93 846.0 0 93 84 135 846.0 0 135 85 117 846.0 0 117 86 48 846.0 0 48 87 33 846.0 0 33 88 28 846.0 0 28 89 108 846.0 0 108 90 207 846.0 0 207 91 242 846.0 0 242 92 217 846.0 0 217 93 550 846.0 0 550 94 112 846.0 0 112 95 225 846.0 0 225 96 225 846.0 0 225 97 191 846.0 0 191 98 220 846.0 0 220 99 186 846.0 0 186 100 225 846.0 0 225 101 348 846.0 0 348 102 908 846.0 0 908 103 886 846.0 0 886 104 500 846.0 0 500 105 136 846.0 0 136 106 138 846.0 0 138 107 78 846.0 0 78 108 121 846.0 0 121 109 451 846.0 0 451 110 422 846.0 0 422 111 460 846.0 0 460 112 273 846.0 0 273 113 283 846.0 0 283 114 279 846.0 0 279 115 400 846.0 0 400 116 199 846.0 0 199 117 143 846.0 0 143 118 132 846.0 0 132 119 88 846.0 0 88 120 114 846.0 0 114 121 107 846.0 0 107 122 339 846.0 0 339 123 437 846.0 0 437 124 398 846.0 0 398 125 694 846.0 0 694 126 223 846.0 0 223 127 328 846.0 0 328 128 283 846.0 0 283 129 339 846.0 0 339 130 352 846.0 0 352 131 304 846.0 0 304 132 387 846.0 0 387 133 258 846.0 0 258 134 241 846.0 0 241 135 274 846.0 0 274 136 430 846.0 0 430 137 833 846.0 0 833 138 835 846.0 0 835 139 1378 846.0 0 1378 140 2320 846.0 0 2320 141 2350 846.0 0 2350 142 51917 846.0 0 51917 143 181349 846.0 0 181349 144 23610 846.0 0 23610 145 406 846.0 0 406 146 285 846.0 0 285 147 506 846.0 0 506 148 408 846.0 0 408 149 346 846.0 0 346 150 220 846.0 0 220 151 205 846.0 0 205 152 148 846.0 0 148 153 195 846.0 0 195 154 132 846.0 0 132 155 723 846.0 0 723 156 435 846.0 0 435 157 441 846.0 0 441 158 334 846.0 0 334 159 227 846.0 0 227 160 351 846.0 0 351 161 304 846.0 0 304 162 3515 846.0 0 3515 163 5772 846.0 0 5772 164 1852 846.0 0 1852 165 197 846.0 0 197 166 135 846.0 0 135 167 440 846.0 0 440 168 663 846.0 0 663 169 373 846.0 0 373 170 198 846.0 0 198 171 137 846.0 0 137 172 130 846.0 0 130 173 158 846.0 0 158 174 96 846.0 0 96 175 202 846.0 0 202 176 479 846.0 0 479 177 482 846.0 0 482 178 395 846.0 0 395 179 181 846.0 0 181 180 141 846.0 0 141 181 144 846.0 0 144 182 132 846.0 0 132 183 213 846.0 0 213 184 345 846.0 0 345 185 251 846.0 0 251 186 185 846.0 0 185 187 146 846.0 0 146 188 231 846.0 0 231 189 290 846.0 0 290 190 338 846.0 0 338 191 171 846.0 0 171 192 177 846.0 0 177 193 176 846.0 0 176 194 167 846.0 0 167 195 217 846.0 0 217 196 229 846.0 0 229 197 277 846.0 0 277 198 433 846.0 0 433 199 146 846.0 0 146 200 196 846.0 0 196 201 93 846.0 0 93 202 161 846.0 0 161 203 350 846.0 0 350 204 288 846.0 0 288 205 258 846.0 0 258 206 346 846.0 0 346 207 275 846.0 0 275 208 208 846.0 0 208 209 209 846.0 0 209 210 296 846.0 0 296 211 326 846.0 0 326 212 278 846.0 0 278 213 243 846.0 0 243 214 351 846.0 0 351 215 298 846.0 0 298 216 370 846.0 0 370 217 322 846.0 0 322 218 253 846.0 0 253 219 208 846.0 0 208 220 140 846.0 0 140 221 130 846.0 0 130 222 257 846.0 0 257 223 422 846.0 0 422 224 586 846.0 0 586 225 257 846.0 0 257 226 221 846.0 0 221 227 361 846.0 0 361 228 218 846.0 0 218 229 199 846.0 0 199 230 206 846.0 0 206 231 188 846.0 0 188 232 237 846.0 0 237 233 338 846.0 0 338 234 338 846.0 0 338 235 346 846.0 0 346 236 240 846.0 0 240 237 233 846.0 0 233 238 243 846.0 0 243 239 208 846.0 0 208 240 240 846.0 0 240 241 167 846.0 0 167 242 159 846.0 0 159 243 294 846.0 0 294 244 351 846.0 0 351 245 365 846.0 0 365 246 447 846.0 0 447 247 247 846.0 0 247 248 148 846.0 0 148 249 48 846.0 0 48 250 64 846.0 0 64 251 145 846.0 0 145 252 251 846.0 0 251 253 435 846.0 0 435 254 221 846.0 0 221 255 421 846.0 0 421 256 353 846.0 0 353 257 351 846.0 0 351 258 356 846.0 0 356 259 381 846.0 0 381 260 261 846.0 0 261 261 218 846.0 0 218 262 486 846.0 0 486 263 401 846.0 0 401 264 303 846.0 0 303 265 233 846.0 0 233 266 388 846.0 0 388 267 133 846.0 0 133 268 227 846.0 0 227 269 162 846.0 0 162 270 109 846.0 0 109 271 210 846.0 0 210 272 228 846.0 0 228 273 279 846.0 0 279 274 212 846.0 0 212 275 230 846.0 0 230 276 183 846.0 0 183 277 153 846.0 0 153 278 190 846.0 0 190 279 292 846.0 0 292 280 200 846.0 0 200 281 186 846.0 0 186 282 272 846.0 0 272 283 351 846.0 0 351 284 397 846.0 0 397 285 310 846.0 0 310 286 331 846.0 0 331 287 301 846.0 0 301 288 110 846.0 0 110 289 96 846.0 0 96 290 78 846.0 0 78 291 122 846.0 0 122 292 557 846.0 0 557 293 450 846.0 0 450 294 204 846.0 0 204 295 258 846.0 0 258 296 191 846.0 0 191 297 138 846.0 0 138 298 110 846.0 0 110 299 150 846.0 0 150 300 255 846.0 0 255 301 377 846.0 0 377 Finished in 11.19 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 322,891 Reads with adapters: 8,690 (2.7%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 8,677 (2.7%) Total basepairs processed: 53,254,105 bp Total written (filtered): 2,363,178 bp (4.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8690 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 1 0 1 19 16 0.0 3 0 16 20 8586 0.0 4 8499 85 1 1 21 17 0.0 4 4 13 22 3 0.0 4 1 0 2 23 1 0.0 4 0 0 0 1 24 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 35 1 0.0 4 1 37 2 0.0 4 0 0 0 0 2 38 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 63 2 0.0 4 0 0 0 2 69 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 127 40 0.0 4 0 0 0 0 40 128 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.30 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 8,677 Reads with adapters: 382 (4.4%) Reads written (passing filters): 8,677 (100.0%) Total basepairs processed: 2,363,178 bp Total written (filtered): 2,337,411 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 382 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 22.3% C: 1.8% G: 75.1% T: 0.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 9 1 0.0 0 0 1 10 29 0.0 1 3 26 11 263 0.0 1 5 258 13 1 0.0 1 0 1 97 1 0.0 1 1 151 1 0.0 1 0 1 224 2 0.0 1 2 260 78 0.0 1 74 4 261 2 0.0 1 2 262 2 0.0 1 2 270 2 0.0 1 2