This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGCGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 88.62 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 149,362 (4.3%) Reads that were too short: 9,215 (0.3%) Reads written (passing filters): 140,147 (4.0%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 38,958,483 bp (3.7%) === Adapter 1 === Sequence: CGCGAT; Type: regular 5'; Length: 6; Trimmed: 149362 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1260 846.0 0 1260 7 53723 846.0 0 53723 8 807 846.0 0 807 9 68357 846.0 0 68357 10 126 846.0 0 126 11 53 846.0 0 53 12 25 846.0 0 25 13 37 846.0 0 37 14 51 846.0 0 51 15 30 846.0 0 30 16 48 846.0 0 48 17 105 846.0 0 105 18 35 846.0 0 35 19 39 846.0 0 39 20 39 846.0 0 39 21 43 846.0 0 43 22 43 846.0 0 43 23 35 846.0 0 35 24 36 846.0 0 36 25 28 846.0 0 28 26 39 846.0 0 39 27 26 846.0 0 26 28 36 846.0 0 36 29 35 846.0 0 35 30 48 846.0 0 48 31 38 846.0 0 38 32 33 846.0 0 33 33 32 846.0 0 32 34 31 846.0 0 31 35 46 846.0 0 46 36 30 846.0 0 30 37 34 846.0 0 34 38 40 846.0 0 40 39 31 846.0 0 31 40 41 846.0 0 41 41 43 846.0 0 43 42 73 846.0 0 73 43 36 846.0 0 36 44 103 846.0 0 103 45 27 846.0 0 27 46 32 846.0 0 32 47 32 846.0 0 32 48 36 846.0 0 36 49 41 846.0 0 41 50 73 846.0 0 73 51 49 846.0 0 49 52 42 846.0 0 42 53 38 846.0 0 38 54 44 846.0 0 44 55 80 846.0 0 80 56 40 846.0 0 40 57 34 846.0 0 34 58 42 846.0 0 42 59 25 846.0 0 25 60 20 846.0 0 20 61 33 846.0 0 33 62 36 846.0 0 36 63 30 846.0 0 30 64 28 846.0 0 28 65 54 846.0 0 54 66 38 846.0 0 38 67 35 846.0 0 35 68 34 846.0 0 34 69 33 846.0 0 33 70 28 846.0 0 28 71 36 846.0 0 36 72 28 846.0 0 28 73 86 846.0 0 86 74 62 846.0 0 62 75 59 846.0 0 59 76 43 846.0 0 43 77 43 846.0 0 43 78 21 846.0 0 21 79 48 846.0 0 48 80 44 846.0 0 44 81 59 846.0 0 59 82 31 846.0 0 31 83 30 846.0 0 30 84 34 846.0 0 34 85 40 846.0 0 40 86 130 846.0 0 130 87 68 846.0 0 68 88 68 846.0 0 68 89 36 846.0 0 36 90 39 846.0 0 39 91 47 846.0 0 47 92 305 846.0 0 305 93 260 846.0 0 260 94 258 846.0 0 258 95 79 846.0 0 79 96 66 846.0 0 66 97 59 846.0 0 59 98 33 846.0 0 33 99 33 846.0 0 33 100 37 846.0 0 37 101 36 846.0 0 36 102 13 846.0 0 13 103 14 846.0 0 14 104 24 846.0 0 24 105 29 846.0 0 29 106 20 846.0 0 20 107 39 846.0 0 39 108 27 846.0 0 27 109 27 846.0 0 27 110 38 846.0 0 38 111 38 846.0 0 38 112 45 846.0 0 45 113 42 846.0 0 42 114 37 846.0 0 37 115 26 846.0 0 26 116 35 846.0 0 35 117 41 846.0 0 41 118 44 846.0 0 44 119 76 846.0 0 76 120 80 846.0 0 80 121 50 846.0 0 50 122 32 846.0 0 32 123 104 846.0 0 104 124 216 846.0 0 216 125 460 846.0 0 460 126 56 846.0 0 56 127 64 846.0 0 64 128 40 846.0 0 40 129 53 846.0 0 53 130 133 846.0 0 133 131 92 846.0 0 92 132 47 846.0 0 47 133 42 846.0 0 42 134 51 846.0 0 51 135 35 846.0 0 35 136 40 846.0 0 40 137 30 846.0 0 30 138 46 846.0 0 46 139 46 846.0 0 46 140 46 846.0 0 46 141 32 846.0 0 32 142 39 846.0 0 39 143 38 846.0 0 38 144 43 846.0 0 43 145 42 846.0 0 42 146 57 846.0 0 57 147 232 846.0 0 232 148 32 846.0 0 32 149 43 846.0 0 43 150 28 846.0 0 28 151 49 846.0 0 49 152 45 846.0 0 45 153 43 846.0 0 43 154 48 846.0 0 48 155 54 846.0 0 54 156 41 846.0 0 41 157 44 846.0 0 44 158 96 846.0 0 96 159 35 846.0 0 35 160 56 846.0 0 56 161 58 846.0 0 58 162 41 846.0 0 41 163 34 846.0 0 34 164 60 846.0 0 60 165 46 846.0 0 46 166 50 846.0 0 50 167 84 846.0 0 84 168 71 846.0 0 71 169 45 846.0 0 45 170 49 846.0 0 49 171 56 846.0 0 56 172 48 846.0 0 48 173 426 846.0 0 426 174 127 846.0 0 127 175 117 846.0 0 117 176 106 846.0 0 106 177 92 846.0 0 92 178 263 846.0 0 263 179 166 846.0 0 166 180 261 846.0 0 261 181 140 846.0 0 140 182 93 846.0 0 93 183 92 846.0 0 92 184 59 846.0 0 59 185 77 846.0 0 77 186 93 846.0 0 93 187 54 846.0 0 54 188 60 846.0 0 60 189 48 846.0 0 48 190 46 846.0 0 46 191 49 846.0 0 49 192 142 846.0 0 142 193 178 846.0 0 178 194 569 846.0 0 569 195 93 846.0 0 93 196 57 846.0 0 57 197 49 846.0 0 49 198 501 846.0 0 501 199 1937 846.0 0 1937 200 1006 846.0 0 1006 201 192 846.0 0 192 202 97 846.0 0 97 203 58 846.0 0 58 204 95 846.0 0 95 205 103 846.0 0 103 206 414 846.0 0 414 207 80 846.0 0 80 208 72 846.0 0 72 209 51 846.0 0 51 210 53 846.0 0 53 211 39 846.0 0 39 212 39 846.0 0 39 213 44 846.0 0 44 214 69 846.0 0 69 215 102 846.0 0 102 216 74 846.0 0 74 217 72 846.0 0 72 218 74 846.0 0 74 219 382 846.0 0 382 220 207 846.0 0 207 221 129 846.0 0 129 222 130 846.0 0 130 223 94 846.0 0 94 224 98 846.0 0 98 225 49 846.0 0 49 226 57 846.0 0 57 227 67 846.0 0 67 228 82 846.0 0 82 229 59 846.0 0 59 230 76 846.0 0 76 231 57 846.0 0 57 232 30 846.0 0 30 233 38 846.0 0 38 234 52 846.0 0 52 235 46 846.0 0 46 236 89 846.0 0 89 237 56 846.0 0 56 238 167 846.0 0 167 239 349 846.0 0 349 240 1165 846.0 0 1165 241 550 846.0 0 550 242 213 846.0 0 213 243 53 846.0 0 53 244 44 846.0 0 44 245 50 846.0 0 50 246 106 846.0 0 106 247 104 846.0 0 104 248 124 846.0 0 124 249 89 846.0 0 89 250 46 846.0 0 46 251 29 846.0 0 29 252 36 846.0 0 36 253 42 846.0 0 42 254 47 846.0 0 47 255 58 846.0 0 58 256 81 846.0 0 81 257 114 846.0 0 114 258 107 846.0 0 107 259 35 846.0 0 35 260 28 846.0 0 28 261 35 846.0 0 35 262 48 846.0 0 48 263 34 846.0 0 34 264 33 846.0 0 33 265 46 846.0 0 46 266 48 846.0 0 48 267 82 846.0 0 82 268 85 846.0 0 85 269 57 846.0 0 57 270 39 846.0 0 39 271 38 846.0 0 38 272 39 846.0 0 39 273 25 846.0 0 25 274 59 846.0 0 59 275 72 846.0 0 72 276 42 846.0 0 42 277 34 846.0 0 34 278 49 846.0 0 49 279 65 846.0 0 65 280 90 846.0 0 90 281 64 846.0 0 64 282 33 846.0 0 33 283 41 846.0 0 41 284 34 846.0 0 34 285 43 846.0 0 43 286 210 846.0 0 210 287 99 846.0 0 99 288 119 846.0 0 119 289 55 846.0 0 55 290 77 846.0 0 77 291 67 846.0 0 67 292 76 846.0 0 76 293 81 846.0 0 81 294 54 846.0 0 54 295 36 846.0 0 36 296 23 846.0 0 23 297 26 846.0 0 26 298 14 846.0 0 14 299 27 846.0 0 27 300 33 846.0 0 33 301 12 846.0 0 12 Finished in 6.39 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 140,147 Reads with adapters: 70,242 (50.1%) Reads that were too short: 68 (0.0%) Reads written (passing filters): 70,174 (50.1%) Total basepairs processed: 38,958,483 bp Total written (filtered): 19,299,560 bp (49.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 70242 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 7 0.0 2 0 0 0 7 15 21 0.0 3 0 0 18 3 16 558 0.0 3 0 528 26 4 17 69247 0.0 3 67439 1603 159 46 18 119 0.0 3 3 98 11 7 19 55 0.0 3 0 2 21 32 20 152 0.0 3 93 3 1 55 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 30 1 0.0 3 1 41 1 0.0 3 0 0 0 1 44 3 0.0 3 0 0 0 3 46 1 0.0 3 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 84 4 0.0 3 0 0 0 4 86 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 95 4 0.0 3 0 0 0 4 105 3 0.0 3 0 0 0 3 108 3 0.0 3 0 0 0 3 109 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 160 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 0 2 221 2 0.0 3 0 0 0 2 223 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 5 0.0 3 0 0 0 5 282 11 0.0 3 0 0 1 10 283 10 0.0 3 0 0 0 10 288 6 0.0 3 0 0 0 6 289 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.48 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 70,174 Reads with adapters: 3,874 (5.5%) Reads written (passing filters): 70,174 (100.0%) Total basepairs processed: 19,299,560 bp Total written (filtered): 19,287,862 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 3874 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 55.7% C: 12.5% G: 28.3% T: 3.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3805 1096.5 0 3805 4 63 274.1 0 63 5 5 68.5 0 5 6 1 17.1 0 1